Effects of raw meat and process procedure on Nε-carboxymethyllysine and Nε-carboxyethyl-lysine formation in meat products

Ligang Yu,Chang Gao,Maomao Zeng,Zhiyong He,Linxiang Wang,Shuang Zhang,Jie Chen 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

This work aimed to investigate the effects of stored raw meat and process procedures onNε-carboxymethyl-lysine (CML) and Nε-carboxyethyl-lysine (CEL) generation in meat products. Meatproducts of raw pork were sterilized and pasteurized at different storage times (0-4 months) and theCML/CEL contents were determined. The results showed that the extent of lipid and protein oxidationof raw pork increased with increasing storage time. A linear correlation was found betweenthiobarbituric acid-reactive substances value/carbonyl content and CML/CEL in sterilized meatproducts, indicating that stored raw pork could promote CML/CEL formation under high temperatureprocessing. Furthermore, mild heating temperatures seemed to favor CML formation, while hightemperature could accelerate CEL generation. Therefore, formation kinetics of CML and CEL might bedifferent for different process procedures. These results suggested that stored raw meat andprocessing temperature could significantly affect CML and CEL generation in meat products.

Transcriptomic view of survival during early seedling growth of the extremophyte <i>Haloxylon ammodendron</i>

Fan, Ligang,Wang, Guannan,Hu, Wei,Pantha, Pramod,Tran, Kieu-Nga,Zhang, Hua,An, Lizhe,Dassanayake, Maheshi,Qiu, Quan-Sheng Elsevier 2018 Vol. No.

<P><B>Abstract</B></P> <P>Seedling establishment in an extreme environment requires an integrated genomic and physiological response to survive multiple abiotic stresses. The extremophyte, <I>Haloxylon ammodendron</I> is a pioneer species capable of colonizing temperate desert sand dunes. We investigated the induced and basal transcriptomes in <I>H. ammodendron</I> under water-deficit stress during early seedling establishment. We find that not only drought-responsive genes, but multiple genes in pathways associated with salt, osmotic, cold, UV, and high-light stresses were induced, suggesting an altered regulatory stress response system. Additionally, <I>H. ammodendron</I> exhibited enhanced biotic stress tolerance by down-regulation of genes that were generally up-regulated during pathogen entry in susceptible plants. By comparing the <I>H. ammodendron</I> basal transcriptome to six closely related transcriptomes in Amaranthaceae, we detected enriched basal level transcripts in <I>H. ammodendron</I> that shows preadaptation to abiotic stress and pathogens. We found transcripts that were generally maintained at low levels and some induced only under abiotic stress in the stress-sensitive model, <I>Arabidopsis thaliana</I> to be highly expressed under basal conditions in the Amaranthaceae transcriptomes including <I>H. ammodendron</I>. <I>H. ammodendron</I> shows coordinated expression of genes that regulate stress tolerance and seedling development resource allocation to support survival against multiple stresses in a sand dune dominated temperate desert environment.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We report the transcriptomic signals of <I>H. ammodendron</I> in response to drought that contribute to plant survival. </LI> <LI> We highlight the transcriptional and biological processes for the survival of <I>H. ammodendron</I> at early developmental stage. </LI> <LI> We find abundant orthologs in extremophytes that are rare in Arabidopsis. </LI> <LI> These orthologs provide novel candidates to discover networks naturally selected as adaptations to environmental stresses. </LI> </UL> </P>

Effects of raw meat and process procedure on N^ε-carboxymethyl-lysine and N^ε-carboxyethyl-lysine formation in meat products

Yu, Ligang,Gao, Chang,Zeng, Maomao,He, Zhiyong,Wang, Linxiang,Zhang, Shuang,Chen, Jie 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

This work aimed to investigate the effects of stored raw meat and process procedures on $N^{\varepsilon}$-carboxymethyl-lysine (CML) and $N^{\varepsilon}$-carboxyethyl-lysine (CEL) generation in meat products. Meat products of raw pork were sterilized and pasteurized at different storage times (0-4 months) and the CML/CEL contents were determined. The results showed that the extent of lipid and protein oxidation of raw pork increased with increasing storage time. A linear correlation was found between thiobarbituric acid-reactive substances value/carbonyl content and CML/CEL in sterilized meat products, indicating that stored raw pork could promote CML/CEL formation under high temperature processing. Furthermore, mild heating temperatures seemed to favor CML formation, while high temperature could accelerate CEL generation. Therefore, formation kinetics of CML and CEL might be different for different process procedures. These results suggested that stored raw meat and processing temperature could significantly affect CML and CEL generation in meat products.

Upright Orientation of 3D shapes via Tensor Rank Minimization

Weiming Wang,Xiuping Liu,Ligang Liu (사)한국CDE학회 2013 한국CAD/CAM학회 국제학술발표 논문집 Vol.2010 No.8

In general, the upright orientation of a model is beneficial for human to recognize this model and is widely used in geometry processing and computer graphics. However, the orientation of the model obtained by existing technologies, such as 3D scanning systems or modeling, may be far away from the right orientation. In order to solve this problem, a robust and efficient upright method is needed. We observe that when the model is aligned with the three axes, the rank of the three-order tensor constructed by this model is the lowest usually. Inspired by this observation, we formulate the alignment of the 3D model with axes as a low-rank tensor optimization problem which is a global and unsupervised method. After the 3D model has been aligned with the three axes, some geometric properties can be used to pick out the best upright orientation from the six candidate supporting bases easily. The three-order tensor is constructed by voxelizing the bounding box of the 3D model, and then filling the voxel element with zero or one based on whether it contains the points of the model or not. The experimental results demonstrate that our method is robust, efficient and effective for all kinds of the models (manifold or non-manifold, man-made or non-artificial, or point cloud).

Upright orientation of 3D shapes via tensor rank minimization

Weiming Wang,Xiuping Liu,Ligang Liu 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.7

In general, the upright orientation of a model is beneficial for human to recognize this model and is widely used in geometry processingand computer graphics. However, the orientation of the model obtained by existing technologies, such as 3D scanning systems ormodeling, may be far away from the right orientation. In order to solve this problem, a robust and efficient upright method is needed. Weobserve that when the model is aligned with the three axes, the rank of the three-order tensor constructed by this model is the lowest usually. Inspired by this observation, we formulate the alignment of the 3D model with axes as a low-rank tensor optimization problemwhich is a global and unsupervised method and the genetic algorithm (GA) is used to solve this optimization problem. After the 3Dmodel has been aligned with the three axes, some geometric properties can be used to pick out the best upright orientation from the sixcandidate supporting bases easily. The three-order tensor is constructed by voxelizing the bounding box of the 3D model, and then fillingthe voxel element with zero or one based on whether it contains the points of the model or not. The experimental results demonstrate thatour method is robust, efficient and effective for all kinds of the models (manifold or non-manifold, man-made or non-artificial, or pointcloud).

A ‘‘micro-explosion” strategy for preparing membranes with high porosity, permeability, and dye/salt separation efficiency

Xinyang Li,Ligang Lin,Zitian Li,Jing Yang,Wensong Ma,Xu Yang,Xiaopeng Li,Chunhong Wang,Qingping Xin,Kongyin Zhao 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.119 No.-

In this study, inspired by ‘‘micro-explosion” strategies, a separation membrane with high porosity andpermeability, and highly efficient separation performance was prepared. With the use of polyvinylidenefluoride (Solvay6015) as membrane material, azodicarbonamide (AC) as an ‘‘explosion center point” tothe casting membrane solution, and NaOH as an ‘‘external stimulus” in the coagulation bath, the twochemicals undergo in-situ foaming reaction to form a loose nanofiltration membrane. FTIR, XPS, andTGA results demonstrated that the decomposition of AC was complete, which produced gases thatincreased the porosity of the membrane. The optimized membrane has a higher flux(101.72 L m2 h1 at 0.3 Mpa), higher negative surface charge, and better mechanical properties underthe premise of separating CR/NaCl. In the separation of pollutants with different molecular weights,the permeation flux of the optimized membrane increased by more than double. This foaming technologywas also applied to another membrane material, ethylene vinyl alcohol, from which we found that themembrane also had higher porosity and better permeability. Together, this paper presents an in-situfoaming method for preparing separation membranes and lays the foundation for solving the trade-offbetween membrane permeability and rejection in dye/salt separation.

Phase II Trial of Epigallocatechin-3-Gallate in Acute Radiation-Induced Esophagitis for Esophagus Cancer

Xiaoling Li,Ligang Xing,Yujun Zhang,Peng Xie,Wanqi Zhu,Xiangjiao Meng,Yinxia Wang,Lingling Kong,Hanxi Zhao,Jinming Yu 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.1

Acute radiation-induced esophagitis (ARIE) is among the most serious form of toxicities associated with definitive radiotherapy or chemoradiotherapy used for treatment of patients with esophageal cancer. Our preliminary phase I and II trials of lung cancer patients who received radiotherapy indicated epigallocatechin-3-gallate (EGCG) as a promising therapeutic option against ARIE. Therefore, we conducted a prospective phase II study to validate the efficacy and safety of EGCG in the treatment of ARIE. The patients who received chemoradiotherapy or definitive radiotherapy for treatment of esophageal cancer in the Shandong Cancer Hospital and Institute in China were enrolled for the present study. EGCG (440 μM) was administered with first onset of ARIE and then at weeks after final radiotherapy. The patients were monitored every week for dysphagia, Radiation Therapy Oncology Group (RTOG) score, and esophagitis-related pain. Moreover, tumor response and the effect on survival following the treatment were also evaluated. Comparison of the RTOG score in the first, second, third, fourth, fifth, and even sixth week after EGCG prescription and the first and second week after radiotherapy with baseline indicates a significant reduction. The tumor response rate was 86.3%. The overall survival rate in 1, 2, and 3 years was found to be 74.5%, 58%, and 40.5%. Oral administration of EGCG solution seems to be feasible for treating ARIE in patients with esophageal cancer who receive radiation therapy. EGCG might be an ARIE-reliever without compromising the efficacy of radiation therapy. A randomized study with a control group is needed for further evaluation.

Coral stone-inspired superwetting membranes with anti-fouling and self-cleaning properties for highly efficient oil–water separation

Wensong Ma,Ligang Lin,Jing Yang,Zitian Liu,Xinyang Li,Meina Xu,Xiaopeng Li,Chunhong Wang,Qingping Xin,Kongyin Zhao 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.120 No.-

Nowadays, the use of separation membranes to deal with oil–water emulsions has gained popularity. However, oil fouling of membrane surfaces during the separation of oil–water emulsion is still a significantchallenge. In this study, inspired by the biological coral stone structure, the gel layer was firmlyattached to the surface of membrane using a simple co-blending and cross-linking strategy. A superwettingmembrane (PVDF/CD-SA) with a coral stone structure was obtained. The PVDF/CD-SA membranehad a high permeate flux that was 4.2 times higher than that of the original membrane and a high separationefficiency of about 99.2 % for the separation of oil–water emulsion. Furthermore, the membranehad outstanding chemical stability. The fluxes of several different oil-in-water emulsions significantlyimproved, and the separation efficiencies were as high as 98 %. Moreover, the separation efficienciesand contact angles of the membrane remained unchanged after numerous cycles of use. The membraneexhibited excellent superhydrophilicity in air (instantaneous water wetting in air) and superoleophobicityunder water (underwater oil contact angle > 156). Most importantly, the oil was able to automaticallydetach from the surface of membrane, resulting in self-cleaning performance. Therefore, this PVDF/CD-SAmembrane eliminated the problem of oil adhesion, exhibiting excellent potential for practical applicationsin oil–water separation.

A second order method for optimal parametrization

Yajuna Li,Guozhao Wang,Ligang Liu (사)한국CDE학회 2007 한국CAD/CAM학회 국제학술발표 논문집 Vol.2007 No.8

Computational and experimental characterization of estrogenic activities of 20(S, R)-protopanaxadiol and 20(S, R)-protopanaxatriol

Tiehua Zhang,Shuning Zhong,Ligang Hou,Yongjun Wang,XiaoJia Xing,Tianzhu Guan,Jie Zhang,Tiezhu Li 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.5

Background: As the main metabolites of ginsenosides, 20(S, R)-protopanaxadiol [PPD(S, R)] and 20(S, R)-protopanaxatriol [PPT(S, R)] are the structural basis response to a series of pharmacological effects of their parent components. Although the estrogenicity of several ginsenosides has been confirmed, however, the underlying mechanisms of their estrogenic effects are still largely unclear. In this work, PPD(S, R) and PPT(S, R) were assessed for their ability to bind and activate human estrogen receptor α(hERα) by a combination of in vitro and in silico analysis. Methods: The recombinant hERα ligand-binding domain (hERa-LBD) was expressed in E. coli strain. The direct binding interactions of ginsenosides with hERα-LBD and their ERα agonistic potency were investigated by fluorescence polarization and reporter gene assays, respectively. Then, molecular dynamics simulations were carried out to simulate the binding modes between ginsenosides and hERα-LBD to reveal the structural basis for their agonist activities toward receptor. Results: Fluorescence polarization assay revealed that PPD(S, R) and PPT(S, R) could bind to hERα-LBD with moderate affinities. In the dual luciferase reporter assay using transiently transfected MCF-7 cells, PPD(S, R) and PPT(S, R) acted as agonists of hERα. Molecular docking results showed that these ginsenosides adopted an agonist conformation in the flexible hydrophobic ligand-binding pocket. The stereostructure of C-20 hydroxyl group and the presence of C-6 hydroxyl group exerted significant influence on the hydrogen bond network and steric hindrance, respectively. Conclusion: This work may provide insight into the chemical and pharmacological screening of novel therapeutic agents from ginsenosides.