Effects of raw meat and process procedure on N^ε-carboxymethyl-lysine and N^ε-carboxyethyl-lysine formation in meat products

Yu, Ligang,Gao, Chang,Zeng, Maomao,He, Zhiyong,Wang, Linxiang,Zhang, Shuang,Chen, Jie 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

This work aimed to investigate the effects of stored raw meat and process procedures on $N^{\varepsilon}$-carboxymethyl-lysine (CML) and $N^{\varepsilon}$-carboxyethyl-lysine (CEL) generation in meat products. Meat products of raw pork were sterilized and pasteurized at different storage times (0-4 months) and the CML/CEL contents were determined. The results showed that the extent of lipid and protein oxidation of raw pork increased with increasing storage time. A linear correlation was found between thiobarbituric acid-reactive substances value/carbonyl content and CML/CEL in sterilized meat products, indicating that stored raw pork could promote CML/CEL formation under high temperature processing. Furthermore, mild heating temperatures seemed to favor CML formation, while high temperature could accelerate CEL generation. Therefore, formation kinetics of CML and CEL might be different for different process procedures. These results suggested that stored raw meat and processing temperature could significantly affect CML and CEL generation in meat products.

Mesenchymal stem cell-derived exosome mir-342-3p inhibits metastasis and chemo-resistance of breast cancer through regulating ID4

Yu Shuyao,Zhou Yuhui,Niu Ligang,Qiao Yan,Yan Yu 한국유전학회 2022 Genes & Genomics Vol.44 No.5

Background: The mesenchymal stem cell-derived exosome (MSCs-exo) carrying microRNAs have been proved to regulate tumor biological activities. Clarifying molecular mechanism and identifying predictive microRNAs will be of great value in anti-tumor therapy improvement. Objective: We aimed to investigate the regulatory role of microRNA-342-3p (miR-342-3p) in MSCs-exo on breast cancer. Methods: Breast cancer tissues and cell lines were used to evaluate miR-342-3p expression in patients with or without lymph node/distal organ metastasis. The impact of MSCs-exo expression on tumor cell chemo-resistance and invasion/migration was measured. Dual-luciferase reporter gene assay was applied to identify binding site. Inhibitor of differentiation 4 (ID4) siRNA and miR-342-3p inhibitor transfection was conducted to further explore the miR-342-3p/ID4 axis on chemo-resistance and metastasis of breast cancer cells. Results: Breast cancer cells revealed significantly lower level of miR-342-3p in patients with metastatic diseases. miR-342-3p suppressed invasive and chemo-resistant behavior of breast cancer tumor cells. Binding site between miR-342-3p and ID4 was proved. ID4 could reverse the influence of miR-342-3p on chemo-resistance. The tumor inhibition effect of IDA siRNA in vivo was also identified. Conclusions: This study demonstrated that miR-342-3p acted as potential tumor suppressor by inhibiting metastasis and chemo-resistance of breast cancer cells through targeting ID4. This study might provide potential therapy targets for the treatment of breast cancer.

Effects of raw meat and process procedure on Nε-carboxymethyllysine and Nε-carboxyethyl-lysine formation in meat products

Ligang Yu,Chang Gao,Maomao Zeng,Zhiyong He,Linxiang Wang,Shuang Zhang,Jie Chen 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.4

This work aimed to investigate the effects of stored raw meat and process procedures onNε-carboxymethyl-lysine (CML) and Nε-carboxyethyl-lysine (CEL) generation in meat products. Meatproducts of raw pork were sterilized and pasteurized at different storage times (0-4 months) and theCML/CEL contents were determined. The results showed that the extent of lipid and protein oxidationof raw pork increased with increasing storage time. A linear correlation was found betweenthiobarbituric acid-reactive substances value/carbonyl content and CML/CEL in sterilized meatproducts, indicating that stored raw pork could promote CML/CEL formation under high temperatureprocessing. Furthermore, mild heating temperatures seemed to favor CML formation, while hightemperature could accelerate CEL generation. Therefore, formation kinetics of CML and CEL might bedifferent for different process procedures. These results suggested that stored raw meat andprocessing temperature could significantly affect CML and CEL generation in meat products.

Phase II Trial of Epigallocatechin-3-Gallate in Acute Radiation-Induced Esophagitis for Esophagus Cancer

Xiaoling Li,Ligang Xing,Yujun Zhang,Peng Xie,Wanqi Zhu,Xiangjiao Meng,Yinxia Wang,Lingling Kong,Hanxi Zhao,Jinming Yu 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.1

Acute radiation-induced esophagitis (ARIE) is among the most serious form of toxicities associated with definitive radiotherapy or chemoradiotherapy used for treatment of patients with esophageal cancer. Our preliminary phase I and II trials of lung cancer patients who received radiotherapy indicated epigallocatechin-3-gallate (EGCG) as a promising therapeutic option against ARIE. Therefore, we conducted a prospective phase II study to validate the efficacy and safety of EGCG in the treatment of ARIE. The patients who received chemoradiotherapy or definitive radiotherapy for treatment of esophageal cancer in the Shandong Cancer Hospital and Institute in China were enrolled for the present study. EGCG (440 μM) was administered with first onset of ARIE and then at weeks after final radiotherapy. The patients were monitored every week for dysphagia, Radiation Therapy Oncology Group (RTOG) score, and esophagitis-related pain. Moreover, tumor response and the effect on survival following the treatment were also evaluated. Comparison of the RTOG score in the first, second, third, fourth, fifth, and even sixth week after EGCG prescription and the first and second week after radiotherapy with baseline indicates a significant reduction. The tumor response rate was 86.3%. The overall survival rate in 1, 2, and 3 years was found to be 74.5%, 58%, and 40.5%. Oral administration of EGCG solution seems to be feasible for treating ARIE in patients with esophageal cancer who receive radiation therapy. EGCG might be an ARIE-reliever without compromising the efficacy of radiation therapy. A randomized study with a control group is needed for further evaluation.

Functional Characterization of Arabidopsis thaliana WRKY39 in Heat Stress

Shujia Li,Xiang Zhou,Ligang Chen,Weidong Huang,Diqiu Yu 한국분자세포생물학회 2010 Molecules and cells Vol.29 No.5

Arabidopsis thaliana WRKY39, a transcription factor that is induced by heat stress, is a member of the group II WRKY proteins and responds to both abiotic and biotic stress. Heat-treated seeds and plants of WRKY39 knock-down mutants had increased susceptibility to heat stress, show-ing reduced germination, decreased survival, and elevated electrolyte leakage compared with wild-type plants. In con-trast, WRKY39 over-expressing plants exhibited enhanced thermotolerance compared with wild-type plants. RT-PCR and qRT-PCR analysis of wrky39 mutants and WRKY39 over-expressing plants identified putative genes regulated by WRKY39. Consistent with a role for WRKY39 in heat tolerance, the expression levels of salicylic acid (SA)-regulated PR1 and SA-related MBF1c genes were down-regulated in wrky39 mutants. In contrast, over-expression of WRKY39 increased the expression of PR1 and MBF1c. The WRKY39 transcript was induced in response to treat-ment with SA or methyljasmonate. Analysis of heat stress-induced WRKY39 in defense signaling mutants, including coi1, ein2, and sid2, further indicated that WRKY39 was positively co-regulated by the SA and jasmonate (JA) sig-naling pathways. Together, these findings reveal that heat stress-induced WRKY39 positively regulates the coopera-tion between the SA- and JA-activated signaling pathways that mediate responses to heat stress.

Functional Characterization of Arabidopsis thaliana WRKY39 in Heat Stress

Li, Shujia,Zhou, Xiang,Chen, Ligang,Huang, Weidong,Yu, Diqiu Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.29 No.5

Arabidopsis thaliana WRKY39, a transcription factor that is induced by heat stress, is a member of the group II WRKY proteins and responds to both abiotic and biotic stress. Heat-treated seeds and plants of WRKY39 knock-down mutants had increased susceptibility to heat stress, showing reduced germination, decreased survival, and elevated electrolyte leakage compared with wild-type plants. In contrast, WRKY39 over-expressing plants exhibited enhanced thermotolerance compared with wild-type plants. RT-PCR and qRT-PCR analysis of wrky39 mutants and WRKY39 over-expressing plants identified putative genes regulated by WRKY39. Consistent with a role for WRKY39 in heat tolerance, the expression levels of salicylic acid (SA)-regulated PR1 and SA-related MBF1c genes were down-regulated in wrky39 mutants. In contrast, over-expression of WRKY39 increased the expression of PR1 and MBF1c. The WRKY39 transcript was induced in response to treatment with SA or methyljasmonate. Analysis of heat stress-induced WRKY39 in defense signaling mutants, including coi1, ein2, and sid2, further indicated that WRKY39 was positively co-regulated by the SA and jasmonate (JA) signaling pathways. Together, these findings reveal that heat stress-induced WRKY39 positively regulates the cooperation between the SA- and JA-activated signaling pathways that mediate responses to heat stress.

연구보문 : SSR 마커를 이용한 녹두 수집자원의 유전적 다양성 분석

( Li Gang ),권순욱 ( Soon Wook Kwon ),최유미 ( Yu Mi Choi ),박용진 ( Yong Jin Park ) 한국국제농업개발학회 2011 韓國國際農業開發學會誌 Vol.23 No.2

본 연구는 중앙아메리카의 니카라과를 포함하여 동아시아, 중앙아시아 지역의 6개 국가에서 수집한 66개 녹두 유전자원에 대하여 15개 SSR marker를 이용하여 유전적 다양성 및 집단의 구조 분석을 통하여 자원보존 및 육종 소재로서의 활용을 위한 기초정보를 제공하고자 본 실험을 수행하였다. 1. 66점의 녹두 자원에 대하여 15개 SSR 마커에 의해 관찰된 allele수는 총 52개로 나타났고, 평균 allele 수는 3.5개 였다. 이들의 gene diversity와 PIC 값은 각각 0.12~0.66, 0.11~0.60 였으며, 평균은 0.35와 0.31로 나타났다. 2. 유전적 다양성을 나타내는 genetic diversity는 국가별로 중국 > 아프가니스탄 > 한국 > 우즈베키스탄 > 일본 > 니카라과 순으로 각각 0.37, 0.36, 0.31, 0.28, 0.24, 0.23으로 나타났으며, 지역적으로 분석한 결과 동아시아지역에서 평균적으로 가장 높은 genetic diversity를 나타났으며, 그 다음으로 중앙아시아, 중앙아 메리카 순으로 나타났다. 3. STRUCTURE를 이용하여 집단의 구조를 분석한 결과, 66 자원 중 54 점(81.8%)이 70%의 확률로 4개의 subpopulation으로 분류되었으며, 12 자원(18.2%)은 혼입된 형태로 분류되었다. 4. Subpopulation의 유전다양성은 4개 나라에서 수집된 7개 자원을 포함하는 S4에서 가장 높게(0.25) 나타났으며, 한국 수집자원 10점과 우즈베키스탄에 수집된 1개 자원이 포함한 S3에서 가장 낮게 나타났다. 5. 각각의 subpopulation은 수집국가에 대한 특성은 가지고 있지 않았으며, S3에서는 한국 수집자원이 다수 포함된 형태로 나타났으나, 대부분의 자원들은 불규칙적으로 분포되었다. This study was conducted to assess the genetic diversity and population structure of 66 mungbean accessions collected from Korea, China, Japan, Afghanistan, Uzbekistan and Nicaragua using 15 simple sequence repeat (SSR) markers. In total, 52 alleles were detected among 66 accessions at all loci with an average of 3.5 alleles per locus. All microsatellite loci were found to be polymorphic. The expected heterozygosity (HE) and polymorphism information content (PIC) ranged from 0.12 to 0.65 (mean = 0.36) and from 0.11 to 0.60 (mean = 0.31). The mean genetic diversity in Korea, China, Japan, Uzbekistan, Afghanistan, and Nicaragua were 0.31, 0.37, 0.24, 0.28, 0.36, and 0.23, respectively. The average genetic diversity showed the following country-wide trend, China > Afghanistan > Korea > Uzbekistan > Japan > Nicaragua. In addition, the region-wide analysis showed that accessions from East Asia possessed the highest average genetic diversity, followed by Central Asia, and Central America. The model-based structure analysis revealed the presence of four subpopulations, which was essentially consistent with clustering based on genetic distance. Fifty-four accessions (81.8%) showed a clear relation to each cluster with their inferred value (> 70%), while, the other 12 accessions (18.2%) were categorized as admixtures. The results explored the genetic diversity of East and Central Asia which will be useful for future germplasm preservation, conservation, and crossbreeding programs for mungbean.