백서 뇌에서 전기경련 충격에 의해 발현이 조절되는 새로운 유전자

정선주,박정은,강웅구,구영진,박주배,김용식 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.5

연구목적: 이 연구는 백서 뇌에서 전기경련 충격(Electroconvulsive shock, ECS)에 의해 발현이 조절되는 새로운 유전자를 검색하고, 발달단게 및 뇌 조직에 따른 발현양상을 관찰하기 위하여 시행되었다. 방법: 생후 7일, 21일된 어린 웅성 백서에 전기경련 충격을 가한 후 mRNS differential display-PCR(DD-PCR) 기법을 시행하여 발현의 차이를 나타내는 유전자를 검색한 후 서열분석을 실시하였다. 생후 7일 및 21일된 백서의 뇌와 성년 백서의 대뇌피질, 해마를 이용한 Northern blot 분석을 시행하여 검색된 유전자의 ECS 처치 후 시간경과에 따른 유전자 발현양상을 관찰하였다. 결과: ECS 처치군과 위충격군간에 발현의 차이를 나타낸 유전자 4개를 검색할 수 있었으며, 이중 ECS에 의해 발현이 증가되는 1개의 유전자를 선정하여 서열분석을 실시한 결과 EST 서열만이 보고된 새로운 유전자임이 밝혀졌다. 이 유전자는 생후 7일된 백서의 뇌에서는 ECS 처치 후 180분에 발현양의 증가가 최고치에 도달하였으나, 생후 21일된 백서의 뇌와 성년백서의 대뇌피질 및 해마에서는 360분 경과시까지 지속적으로 발현 양이 증가하였다. 결론: 이같은 결과는 ECS에 의해 생체 내에서 유전자 발현의 조절이 이루어짐을 시사하며, 향후 이러한 유전자의 세포 내 기능에 관한 연구를 시행함으로써, ECS에 의한 생체의 반응과 치료기전에 대한 이해를 넓힐 수 있을 것으로 기대된다. 중심단어:전기경련 충격·DD-PCR·유전자·백서 뇌. Objectives: This study was performed to identify genes regulated by electroconvulsive shock (ECS) and to observe the pattern of expression of genes according to different developmental stages and brain regions. Method: ECS(130V, 0.5 sec) was given to male Sprague-Dawley rats with age of postnatal day 7 and 21(P7, P21 respectively). After screening genes regulated by ECS with mRNA diffe-rential display-PCR(DD-PCR), we selected one clone among them and observed the induction of this gene after ECS by time-dependent Northern blot analysis of rat brain of P7, P21 and adult rat cortex and hippocampus. Results: By DD-PCR method, we have identified four clones whose expression was regulated by ECS. Among them, one(CP 10-2) was proved to be a new gene by sequencing and BLAST search. Its expression was increased after ECS in P7, P21, and adult rat brain. The expression of CP 10-2 reached peak level at 180 minutes after ECS in P7 rat brain, but was further increased until 360 minutes after ECS in P21 and adult rat brain. Conclusion: In this study, a new gene was identified in rat brain which showed up-regulated expression in response to ECS. Cloning and characterization of this new gene would be helpful to elucidate the effect of ECS in rat brain. KEY WORDS:Electroconvulsive shock·Differential display-PCR·Gene·Rat brain.

TRAM Flap Reconstruction

Ung Sik Jin 대한외과학회 2015 대한외과학회 학술대회 초록집 Vol.2015 No.11

Identification of Zygotic and Nucellar Mandarin Seedlings Using Randomly Amplified Polymorphic DNA

Jin-Ung Yun,Hee Beom Yang,Yong-Hwan Jung,Su-Hyun Yun,Kwang Sik Kim,Chan-Shick Kim,Kwan Jeong Song 한국원예학회 2007 Horticulture, Environment, and Biotechnology Vol.48 No.3

The study aimed at evaluating an efficiency of cross breeding in ‘Miyagawa Wase’ and ‘Okitsu Wase’ satsuma mandarin and ‘Shiranuhi’ mandarin through investigating the seed and seedlings formation, the frequency and position of zygotic seedlings, and the influence of pollen parents using randomly amplified polymorphic DNA (RAPD). Seedlings were in vitro cultured with seeds obtained from four crosses of ‘Miyagawa Wase’ (Citrus unshiu) × ‘Ponkan’ (C reticulata), ‘Okitsu Wase’ (C unshiu) × ‘Swingle Citrumelo’ (C. paradisi × Poncirus trifoliate), ‘Shiranuhi’ [(C unshiu × C. sinensis) × C. reticulata] × ‘Ponkan’ (C. reticulata), and ‘Shiranuhi’ [(C. unshiu × C. sinensis) × C. reticulata] × ‘Swingle Citrumelo’ (C. paradisi × P. trifoliate). RAPD analysis was conducted with five primers selected from screening 77 primers based on DNA amplification of parent plants. Seed formation including total seeds and full sized seeds per fruit between ‘Miyagawa Wase’ and ‘Okitsu Wase’ was not different statistically. However, seed formation of ‘Shiranuhi’ mandarin was significantly affected by pollen genotype. Average seedlings number per seed was not different effectively. The frequency and positioning patterns of zygotic seedling were different in each cultivar. The results suggest that seed formation, polyembryony, and frequency and positioning of zygotic seedling in some citrus having polyembryony might be affected by seed and pollen parents.

Poster Session 1 : ECprofiler: candidate gene search system for ECgene and UniGene

( Bum Jin Kim ),( Seung Yoon Nam ),( Ung Sik Yu ),( Sang Soo Kim ),( Seo Kmin Shin ),( Sang Hyuk Lee ) 한국생화학분자생물학회 (구 한국생화학회) 2005 생화학분자생물학회 춘계학술발표논문집 Vol.2005 No.-

CT Guide를 이용한 간농양의 경피적 천자 배액법

박웅채(Ung Chae Park),한진우(Jin Woo Han),이한식(Han Sik Lee),김윤(Yun Kim) 대한외과학회 1984 Annals of Surgical Treatment and Research Vol.27 No.3

운영비용을 고려한 자전거 쉐어링의 최적 재고수준

김진식(Jin-Sik Kim),이철웅(Chul-Ung Lee) 한국컴퓨터정보학회 2015 韓國컴퓨터情報學會論文誌 Vol.20 No.1

본 논문에서는 자전거 쉐어링 사업을 중심으로 창원시 누비자 자전거 쉐어링 서비스의 최적 재고 수준을 유지하며 시스템 운영비용을 최소화하는 방법을 제안한다. 쉐어링 서비스란 일반적으로 시간단위로 차량을 렌트하는 개념으로 렌터카보다 편리한 서비스로 이용자의 수가 많아지고 있는 추세이다. Vehicle sharing service는 다음 세대 차량과 함께 sustainable transportation 의 수단으로써 급부상하고 있다. 지구 온난화의 심화와 함께 이산화탄소 배출량의 감소에 관심이 집중되면서 기존의 차량의 내연 기관을 하이브리드 혹은 전기차로 변화하고 있으며, 기존의 대중교통 시스템을 다각화로 발전하고 있다. 그 중 실현 가능성이 높은 분야인 자전거 쉐어링 분야이며, 이미 지방 자치 단체 중심으로 자전거 쉐어링 사업이 이루어지고 있다. 본 연구에서는 자전거 쉐어링 서비스의 각 터미널간 최적 자전거 보유수준(S-s)을 제안하는 시뮬레이션을 설계하여 운영비용을 최소화시키는 자전거 터미널의 재고수준을 제안하였다. In this paper, it shows optimal inventory level of bicycle sharing service terminal by maintaining the lowest costs. As the interest to environment and exhaustion of resource increases globally, investment to sustainable transportation increases around advanced countries and interest to efficient transportation, managing and consuming of vehicle increases also. Vehicle sharing service is a model of rental car where customer rent cars for short periods of time often by the hour and its users are increasing for the reason that it is more convenient than car rent. In addition, bicycle sharing service is one of the major parts in vehicle sharing program and many of country are already managing it. This paper proposes optimal inventory levels of vehicle sharing service"s terminal by using simulation calculating operation costs of vehicle sharing service.

Pathophysiological Regulation of Vascular Smooth Muscle Cells by Prostaglandin F<SUB>2α</SUB>-dependent Activation of Phospholipase C-β3

Ki Ung Kang(강기웅),Jun Young Oh(오준영),Yun Ha Lee(이윤한),Hye Sun Lee(이혜선),Seo Yeon Jin(진서연),Sun Sik Bae(배순식) 한국생명과학회 2018 생명과학회지 Vol.28 No.12

죽상동맥경화는 대동맥의 만성염증에 의해 주로 발병되는 폐쇄동맥질환이다. 혈관평활근세포의 증식 및 이동은 죽상동맥경화 발병의 주된 병리적 반응이다. 본 연구에서는 죽상동맥경화 발병기전을 유도하는 표적 염증반응물질의 탐색 및 이들에 의한 신호전달 기전을 연구하였다. 혈관평활근세포의 증식 및 이동은 prostaglandin F2α (PGF2α)에 의해 의미 있게 증가하였으나 tumor necrosis factor α (TNFα)에 의해서는 증가하지 않았다. Prostacyclin I₂ (PGI₂)는 혈관평활근세포의 증식은 촉진시켰으나 이동은 오히려 억제하였다. prostaglandin D₂ (PGD2) 및 prostaglandin E₂ (PGE₂)는 혈관평활근세포의 증식을 촉진시켰으나 이동에는 영향을 미치지 않았다. PGF2α는 용량 의존적으로 혈관평활근세포의 증식 및 이동을 촉진시켰고 EC50는 약 0.1 μM로 관찰되었다. 혈관평활근세포에서 phospholipase C-β3 (PLC-β3) 아형의 발현은 매우 높았으나 PLC-β1, PLC-β2, 및 PLC-β4의 발현은 관찰되지 않았다. U73122 처리를 통해 PLC의 활성을 억제하면 PGF2α에 의한 혈관평활근세포의 이동이 억제되었다. 또한 PLC-β3의 발현을 억제하면 PGF2α에 의한 혈관평활근세포의 증식 및 이동이 억제되었다. 이러한 결과들을 바탕으로 PGF2α 는 혈관평활근세포의 증식 및 이동에 중요한 역할을 수행하고, 여기에는 PLC-β3가 필수적인 역할을 담당하고 있음을 제안한다. Atherosclerosis is an obstructive vessel disease mainly caused by chronic arterial inflammation to which the proliferation and migration of vascular smooth muscle cells (VSMCs) is the main pathological response. In the present study, the primary responsible inflammatory cytokine and its signaling pathway was investigated. The proliferation and migration of VSMCs was significantly enhanced by the prostaglandin F2α (PGF2α), while neither was affected by tumor necrosis factor α. Prostacyclin I₂ was seen to enhance the proliferation of VSMCs while simultaneously suppressing their migration. Both prostaglandin D₂ and prostaglandin E₂ significantly enhanced the migration of VSMCs, however, proliferation was not affected by either of them. The proliferation and migration of VSMCs stimulated by PGF2α progressed in a dose-dependent manner; the EC50 value of both proliferation and migration was 0.1 μM. VSMCs highly expressed the phospholipase isoform C-β3 (PLC-β3) while others such as PLC-β1, PLC-β2, and PLC-β4 were not expressed. Inhibition of the PLCs by U73122 completely blocked the PGF2α-induced migration of VSMCs, and, in addition, silencing PLC-β3 significantly diminished the PGF2α-induced proliferation and migration of VSMCs. Given these results, we suggest that PGF2α plays a crucial role in the proliferation and migration of VSMCs, and activation of PLC-β3 could be involved in their PGF2α-dependent migration.

성인형호흡장애증후군을 동반한 속립성결핵 1 예 보고

김진호,김한일,강종식,박종하,이준상,정철호,전건웅 대한내과학회 1986 대한내과학회지 Vol.31 No.4

Miliary tuberculosis is an unusual cause of adult respiratory distress syndrome. In this case, We described one patient with miliary tuberculosis who developed adult respiratory distress syndrome. The chest radiography on admission was normal. During hospitalization repeated chest X-rays revealed a miliary pattern followed by diffuse pulmonary infiltration, and severe respiratory distress developed. The disgnosis of tuberculosis was suspected early, appropriate therapy was initiated, and he survived. The experience with this case serves to emphasize that miliary tuberculosis should be considered in patients presenting with adult respiratory di8tress syndrome of unknown causes.

GROWTH PATTERNS OF JUVENILE SEAHORSES Hippocampus mohnikei (Bleeker, 1853), FROM KOREA

Young-Ung Choi,Dae-Won Lee,Kyun-Woo Lee,Yoon-Chil Kim,Soo-Jin Heo,Heung-Sik Park 한국수산과학회 양식분과 2015 한국수산과학회 양식분과 학술대회 Vol.2015 No.5

RF 마그네트론 스퍼터링법에 의한 SCT 박막의 제조 및 특성

김진사(Jin Sa Kim),백봉현(Bong Hyun Paek),김충혁(Chung Hyeok Kim),최운식(Woon Sik Choi),박용필(Yong Pil Park),박건호(Geon Ho Park),이준웅(Joon Ung Lee) 한국전기전자재료학회 1998 한국전기전자재료학회 춘계학술대회논문집 Vol.1998 No.0

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