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- 저자 : Tripathy A. (검색결과 36 건)
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A robust enzymeless glucose sensor based on CuO nanoseed modified electrodes
Ahmad, Rafiq,Tripathy, Nirmalya,Hahn, Yoon-Bong,Umar, Ahmad,Ibrahim, Ahmed A.,Kim, S. H. The Royal Society of Chemistry 2015 Dalton Transactions Vol.44 No.28
<P>Herein, we demonstrate the fabrication of a robust enzymeless glucose sensor based on CuO nanoseeds (CNSs) synthesized at low-temperature. The as-fabricated sensor exhibited excellent electrocatalytic ability in a wide-linear range and was further employed for the glucose concentration determined in freshly drawn mice whole blood and serum samples.</P> <P>Graphic Abstract</P><P>CuO nanoseeds were synthesized <I>via</I> a low-temperature aqueous route, for the fabrication of a robust enzymeless glucose sensor. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5dt01664a'> </P>
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A. Tripathy,S. N. Das,S. Bhuyan,R. N. P. Choudhary 한국전기전자재료학회 2019 Transactions on Electrical and Electronic Material Vol.20 No.3
In this communication, the systematic studies of structural (basic crystal data, microstructure), and electrical (dielectric constant and loss, impedance, modulus, conductivity, etc.) properties of the double perovskite material Bi 2 MnCdO 6 have been reported. The compound has been synthesized by means of a high temperature route (a solid state reaction method). From the X-ray diff raction structural investigation, a stable phase of the orthorhombic crystal system has been recognized. The microstructure obtained from the scanning electron microscope shows uniform, dense and compact grain distribution. Detailed investigation of dielectric as well as electrical parameters of the fabricated compound through a broad frequency range (i.e. 1 kHz–1 MHz) and temperature (i.e., 25 °C to 300 °C) have provided many interesting data and results in order to understand its ferroelectric and electrical relaxation mechanism. It has been experimentally affi rm that this material (Bi 2 MnCdO 6 ) has more advantages like high relative dielectric constant and low tangent loss over Bi 2 MnCoO 6 material.
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The Millimeter Continuum Size-Frequency Relationship in the UZ Tau E Disk
Tripathi, Anjali,Andrews, Sean M.,Birnstiel, Tilman,Chandler, Claire J.,Isella, Andrea,Pé,rez, Laura M.,Harris, R. J.,Ricci, Luca,Wilner, David J.,Carpenter, John M.,Calvet, N.,Corder, S. A.,Del American Astronomical Society 2018 The Astrophysical journal Vol.861 No.1
<P>We present high spatial resolution observations of the continuum emission from the young multiple star system UZ Tau at frequencies from 6 to 340 GHz. To quantify the spatial variation of dust emission in the UZ Tau E circumbinary disk, the observed interferometric visibilities are modeled with a simple parametric prescription for the radial surface brightnesses at each frequency. We find evidence that the spectrum steepens with radius in the disk, manifested as a positive correlation between the observing frequency and the radius that encircles a fixed fraction of the emission (R-eff proportional to nu(0.34 +/- 0.08)). The origins of this size-frequency relation are explored in the context of a theoretical framework for the growth and migration of disk solids. While that framework can reproduce a similar size-frequency relation, it predicts a steeper spectrum than that observed. Moreover, it comes closest to matching the data only on timescales much shorter (<= 1 Myr) than the putative UZ Tau age (similar to 2-3 Myr). These discrepancies are direct consequences of the rapid radial drift rates predicted by models of dust evolution in a smooth gas disk. One way to mitigate that efficiency problem is to invoke small-scale gas pressure modulations that locally concentrate drifting solids. If such particle traps reach high-continuum optical depths at 30-340 GHz with a similar to 30%-60%. filling fraction in the inner disk (r less than or similar to 20 au), they can also explain the observed spatial gradient in the UZ Tau E disk spectrum.</P>
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Misra, A.K.,Karim, S.A.,Verma, D.L.,Mishra, A.S.,Tripathi, M.K. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.12
A study was conducted to compare the feeding value of urea treated and untreated mustard straw (MS) for sheep. Treated MS was prepared by adding urea-N at 1.84% and followed by packing in a pit silo for 21 days. Two groups of six empty Avikaline ewes were fed untreated (UTMS) and treated (TMS) mustard straw along with 200 g concentrate per head daily for 90 days. Untreated MS had 0.41% N and the urea treatment increased its N value to 1.58 %. The cell wall constituents were decreased in the TMS except for cellulose which remained unaffected. Dry matter intake of TMS was consistently higher than that of UTMS. Digestibility of DM, OM and fibre fractions of MS improved by the urea treatment. Ewes in both groups were in positive N balance while % N retention was lower in UTMS (26.30%) than in TMS (52.14%). The TMS fed group on average consumed 30.2 g DM, 2.9 g digestible crude protein and $0.2MJ\;DE\;per\;kg\;BW\;day^{-1}$ and maintained their weight whereas, the UTMS fed ewes lost weight. The VFA concentration in rumen liquor was higher in TMS than in UTMS. Total-N, ammonia-N and TCA-precipitable-N were also higher in TMS fed ewes. Blood glucose concentrations in the two groups were similar at initiation of the study. However the glucose concentration of UTMS fed group was significantly (p<0.01) lower than those fed UTMS at the termination of the study. Urea-N concentration was also higher in TMS fed group after 90 days of feeding period. It is concluded that urea treatment of MS improved N value of MS from 0.41% to 1.58% along with sizable improvement in nutritive value and in conjunction with 200 g concentrate, TMS can serve as maintenance ration for sheep. ($ME_{lakt}/ME_{m}=1.46$).
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Soil pH and biome are both key determinants of soil archaeal community structure
Tripathi, B.M.,Kim, M.,Tateno, R.,Kim, W.,Wang, J.,Lai-Hoe, A.,Ab. Shukor, N.A.,Rahim, R.A.,Go, R.,Adams, J.M. Pergamon Press ; Elsevier Science Ltd 2015 Soil biology & biochemistry Vol.88 No.-
The mechanisms underlying community composition and diversity of soil archaea are poorly understood. We compared both total archaea and ammonia oxidizing archaea (AOA) using 16S rRNA and amoA genes pyrosequencing respectively, in two different biomes: tropics (Malaysia), and temperate (Korea and Japan). Despite differences in characteristics of these biomes, we found that at the broad taxonomic level the dominant archaeal lineages are the same, except in certain instances (16S rRNA gene: group 1.1a Thaumarchaeota; amoA gene: Nitrososphaera and Nitrosotalea lineages). However, at the OTU level, both total archaea and AOA communities showed biome-specific patterns, indicating that at lower taxonomic levels biome differences are also important. In both biomes, total archaeal diversity showed a negative correlation with pH, but a hump-shaped curve for AOA diversity, peaking at ~pH 6.0. Within each biome, pH also emerged as the delimiting factor determining variation in community composition of both total archaea and AOA. Communities from each biome clustered separately, even at analogous pH levels. At the OTU level, certain shared OTUs did occur at approximately the same pH range in both biomes. We found that closely related OTUs of both total archaea and AOA respectively tended to co-occur, suggesting that in evolutionary terms these closely related lineages have conserved very similar ecological requirements. This predictability also strongly suggests that soil archaeal community assembly has strongly deterministic aspect. Overall, our findings emphasize that soil archaeal communities are to large extent predictable and structured by both biome and by soil chemical environment, especially pH.
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Tripathy A.,Bhuyan Satyanarayan,Das S. N.,Choudhary R. N. P. 한국세라믹학회 2023 한국세라믹학회지 Vol.60 No.2
A double perovskite ceramic electronic sample of Bi2MnFeO6 has been formulated and synthesized using an ordinary solid-state sintering route from the metal oxides of bismuth, manganese, and iron. The crystallographic structure, morphological, dielectric, electrical, and current–voltage parameters have been analyzed for the fabricated sample. Room temperature XRD (X-ray diffraction) investigation confirms the monoclinic phase. The microstructure obtained from scanning electron microscope reveals compact grain structures with little or no voids. The impedance as well as its electrical properties have been appraised through the impedance analyzer (LCR meter) for a wide range of temperatures and frequencies (30–230 °C and 1 kHz–1 MHz). The negative temperature coefficient of the material is derived from the impedance analysis. The non-ohmic behavior of the sample was confirmed by the current versus voltage (V–I) characteristic of the sample. Most of the significant physical and chemical properties linked with this compound may be critical in the development of future peer group electrical and electronic devices.
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Tripathi, A.,Choi, S. S.,Sherman, D. H.,Kim, E. S. SPRINGER 2016 Journal of industrial microbiology & biotechnology Vol.43 No.8
<P>Tautomycetin (TMC) is a linear polyketide metabolite produced by Streptomyces sp. CK4412 that has been reported to possess multiple biological functions including T cell-specific immunosuppressive and anticancer activities that occur through a mechanism of differential inhibition of protein phosphatases such as PP1, PP2A, and SHP2. We previously reported the characterization of the entire TMC biosynthetic gene cluster constituted by multifunctional type I polyketide synthase (PKS) assembly and suggested that the linear form of TMC could be generated via free acid chain termination by a narrow TMC thioesterase (TE) pocket. The modular nature of the assembly presents a unique opportunity to alter or interchange the native biosynthetic domains to produce targeted variants of TMC. Herein, we report swapping of the TMC TE domain sequence with the exact counterpart of the macrocyclic polyketide pikromycin (PIK) TE. PIK TE-swapped Streptomyces sp. CK4412 mutant produced not only TMC, but also a cyclized form of TMC, implying that the bioengineering based in vivo custom construct can be exploited to produce engineered macrolactones with new structural functionality.</P>
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Tripathi, V. K.,Kumar, V.,Pandey, A.,Vatsa, P.,Dhasmana, A.,Singh, R. P.,Appikonda, S. H.,Hwang, I.,Lohani, M. Humana Press 2017 Molecular Neurobiology Vol. No.
<P>Expression of various cytochrome P450s (CYPs) in mammalian brain cells is well documented. However, such studies are hampered in neural/glial cells of human origin due to nonavailability of human brain cells. To address this issue, we investigated the expression and inducibility of CYP2C8 and CYP3A4 and their responsiveness against cyclophosphamide (CPA) and organophosphorus pesticide monocrotophos (MCP), a known developmental neurotoxicant in human neural (SH-SY5Y) and glial (U373-MG) cell lines. CPA induced significant expression of CYP2C8 and CYP3A4 in both types of cells in a time-dependent manner. Neural cell line exhibited relatively higher constitutive and inducible expression of CYPs than the glial cell line. MCP exposure alone could not induce the significant expression of CYPs, whereas the cells preexposed to CPA showed a significant response to MCP. Similar to the case of CPA induced expressions, neural cells were found to be more vulnerable than glial cells. Our data indicate differential expressions of CYPs in cultured human neural and glial cell lines. The findings were synchronized with protein ligand docking studies, which showed a significant modulatory capacity of MCP by strong interaction with CYP regulators-CAR and PXR. Similarly, the known CYP inducer CPA has also shown significant high docking scores with the two studied CYP regulators. We also observed a significant induction in reactive oxygen species (ROS), lipid peroxides (LPO), micronucleus (MN), chromosomal aberration (CA), and reduction in reduced glutathione (GSH) and catalase following the exposure of MCP. Moreover, the expressions of apoptotic markers such as caspase-3, caspase-9, Bax, and p53 were significantly upregulated, whereas the levels of antiapoptotic marker, Bcl2, was downregulated after the exposure of MCP in both cell lines. These findings confirm the involvement of ROS-mediated oxidative stress, which subsequently triggers apoptosis pathways in both human neural (SH-SY5Y) and glial (U373-MG) cell lines following the exposure of MCP.</P>
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A. Tripathy,S. N. Das,S. Bhuyan,R. N. P. Choudhary 한국전기전자재료학회 2019 Transactions on Electrical and Electronic Material Vol.20 No.2
In this communication, the systematic studies of structural (basic crystal data, microstructure), and electrical (dielectric constant and loss, impedance, modulus, conductivity, etc.) properties of the double perovskite material Bi 2 MnCdO 6 have been reported. The compound has been synthesized by means of a high temperature route (a solid state reaction method). From the X-ray diff raction structural investigation, a stable phase of the orthorhombic crystal system has been recognized. The microstructure obtained from the scanning electron microscope shows uniform, dense and compact grain distribution. Detailed investigation of dielectric as well as electrical parameters of the fabricated compound through a broad frequency range (i.e. 1 kHz–1 MHz) and temperature (i.e., 25 °C to 300 °C) have provided many interesting data and results in order to understand its ferroelectric and electrical relaxation mechanism. It has been experimentally affi rm that this material (Bi 2 MnCdO 6 ) has more advantages like high relative dielectric constant and low tangent loss over Bi 2 MnCoO 6 material.
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