http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Production of anthraquinone rhamnosides in Escherichia coli
Trang Thi Huyen Nguyen,Prakash Parajuli,Ramesh Prasad Pandey,Jae Kyung Sohng 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.01
Rhamnose is naturally occurring deoxy-sugar commonly bound to other sugars in nature. It is a common glycogenic component of glycosides in many plants and microbes. Indigenously available TDP-L-rhamnose from E. coli can be conjugated to natural products using specific glycosyltransferases. Current research focus on the enhancement in production of such potential anthraquinone deoxy-sugar conjugated products through microbial engineering and biotransformation approach. A recombinant E. coli strain was designed by expressing rhamnosyltransferase and anthraquinones were fed exogenously to produce several anthraquionone rhamnose-conjugated derivatives. Among all anthraquinones; Quinizarin, 1,4-dihydroxy-9,10-anthraquinone was efficiently bio-transformed yielding high production. These anthraquinones have important applications in the field of medicine, such as inhibitory effects against human intestinal bacteria. In addition, quinizarin exhibits inhibitory effects in the aflatoxin B1 biotransformation to the corresponding 8, 9-epoxides, which is responsible for the toxic and carcinogenic effects of aflatoxins.
Biosynthesis of Rhamnosylated Anthraquinones in Escherichia coli
Trang Thi Huyen Nguyen,신희정,Ramesh Prasad Pandey,정혜진,류광경,송재경 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.3
Rhamnose is a naturally occurring deoxysugar present as a glycogenic component of plant and microbial natural products. A recombinant mutant Escherichia coli strain was developed by overexpressing genes involved in the TDP-L-rhamnose biosynthesis pathway of different bacterial strains and Saccharothrix espanaensis rhamnosyl transferase to conjugate intrinsic cytosolic TDP-L-rhamnose with anthraquinones supplemented exogenously. Among the five anthraquinones (alizarin, emodin, chrysazin, anthrarufin, and quinizarin) tested, quinizarin was biotransformed into a rhamoside derivative with the highest conversion ratio by whole cells of engineered E. coli. The quinizarin glycoside was identified by various chromatographic and spectroscopic analyses. The anti-proliferative property of the newly synthesized rhamnoside, quinizarin-4-O-α-L-rhamnoside, was assayed in various cancer cells.
Acute Myocardial Infarction in Patients With Single Coronary Artery: A Case Report
Nguyen Thi Huyen,Nguyen Ngoc Trang,Nguyen Khoi Viet,Le Thi Thuy Lien,Hoang Thi Van Hoa,Phung Bao Ngoc,Vu Dang Luu 아시아심장혈관영상의학회 2022 Cardiovascular Imaging Asia Vol.6 No.2
A single coronary artery (SCA) is a rare congenital anomaly. In most cases, it is an incidental finding on coronary angiography and has no clinical significance. However, it can cause angina, myocardial infarction, or even sudden death. Reports of SCA with acute myocardial infarction are very rare in the medical literature. This case study presents a patient with SCA from the right aortic sinus with severe stenosis in the proximal and distal part of the right coronary artery, which was detected using cardiac angiography and cardiac multidetector computed tomography (MDCT) with acute myocardial infarction.
Truong Duong Thuy Thi,Tran Trang Huyen Thi,Nguyen Tam Thanh Thi,Tran Van Hong Thi 한국영양학회 2022 Nutrition Research and Practice Vol.16 No.5
BACKGROUND/OBJECTIVES Although children of ethnic minority groups are experiencing a transition from a predominance of undernutrition to overnutrition, there is little evidence of a dual-malnutrition burden. Therefore, this study examined the double burden of malnutrition among school-aged ethnic minority children living in mountainous areas and its association with their diets. SUBJECTS/METHODS A cross-sectional study was conducted from September 2019 to March 2020 in 3 mountainous areas of Northern Vietnam among 1,556 ethnic minority school-aged children. The prevalence of under-nutrition (stunting and thinness) and over-nutrition (overweight and obesity) were measured using the WHO 2006 child growth standards (height-for-age and BMI-for-age Z-score). Nutritional practices were evaluated by the frequency of food consumption based on a 4-level scale. RESULTS The percentage of children with stunting and thinness were 14.0% and 5.4%, respectively, while the figure for overweight/obesity was 9.4%. The factors positively associated with stunting were living in a family with more than 2 children or being Muong/other ethnicities compared to the Tay ethnicity. Children who consumed fish/shrimps/crabs or milk weekly/daily were less likely to be undernourished compared to those who never consumed these foods. By contrast, children who never consumed foods rich in vitamin A precursors and vitamin A and fruit or consumed daily snacks/junk food were more likely to be overweight/obese. CONCLUSIONS Undernutrition remains a common issue among school-aged children and adolescents of ethnic minority groups, while over-nutrition exists simultaneously. Public health nutrition programs promoting adequate diets and positive lifestyle changes related to nutrition are essential to tackle the double burden of malnutrition among ethnic minority children.
Investigation of Magnetic Anisotropy in Ultra-thin Co Films Grown on 2-D Materials
Pham Trang Huyen Cao,Nguyen Dan Chi,Thi-Nga Do,Soo Min Kim,Chanyong Hwang,Tae Hee Kim 한국자기학회 2021 한국자기학회 학술연구발표회 논문개요집 Vol.31 No.2
2D materials have unique physical and chemical properties which can be used for spintronic device applications, such as the long spin relaxation time of graphene. Additionally, the related heterostructures provide the unprecedented probability of combining the different characteristics via the proximity effect. In this work, ultra-thin Co films were grown on CVD-grown Gr and h-BN layers using the UHV-MBE system. In order to characterize unconventional magnetic properties of 6 ~ 8 nm thick Co films, anisotropy of magnetic tunnel junctions (MTJs) was investigated. The MTJs of x-nm Co (x = 6 and 8)\1.6-nm MgO\12-nm Co were prepared using UHV-MBE system on CVD-grown Gr and h-BN. Perpendicular magnetic anisotropy (PMA) was clearly shown at room temperature (RT) in all the Co bottom electrodes prepared on both graphene and h-BN. Interestingly, for the MTJs grown on h-BN, a very large MR value of more than 700%, was observed with the large PMA, while a few tenth percent of MR values were measured for the Co films grown on Gr layers. Considering theoretical report of an active role of the Co/graphene interface in the magnetism of Co, which allows us to sustain PMA, analysis of interface properties between Co and 2-D materials layers is a prerequisite for understanding their magnetic properties. The development of high-quality ferromagnetic thin films in contact with 2D materials is a state-of-the-art growth technique, whereas only thick polycrystalline or three-dimensional morphologies have been demonstrated so far. We report on the growth of flat, epitaxial ultrathin Co films on h-BN and graphene using UHV-MBE deposition technique.
Tran Thi Huyen,Ha Phuong Trang,Nguyen Thi-Ngan,Bui Dinh-Thanh,Le Pham Tan Quoc,Trinh Ngoc Nam 한국수산과학회 2023 Fisheries and Aquatic Sciences Vol.26 No.3
The thermolabile haemolysin (tlh) of Vibrio parahaemolyticus (Vptlh) from V. parahaemolyticus is a multiple-function enzyme, initially describes as a haemolytic factor activated by lecithin and phospholipase A2 enzymatic activity (Shinoda, 1991; Vazquez-Morado, 2021; Yanagase et al., 1970). Until now, the tlh structure has hypothesized including N-terminal and C-terminal domain, but what domain of the Vptlh structure does the haemolytic activity has not been refined yet. In this study, a 450-bp VpTLH nucleotide sequence of the entire Vptlh gene encoded the C-terminal domain cloned firstly to examine its responsibility in the activity of the Vptlh. The C-terminal domain fused with a 6-His-tag named the His-tag-VpC-terminal domain was expressed successfully in soluble form in the BL21 (DE3) PlysS cell. Remarkably, both expression and purification results confirmed a high agreement in the molecular weight of the His-tag-VpC-terminal domain was 47 kDa. This work showed the His-tag-VpC-terminal domain lysed the erythrocyte membranes in the blood agar and the phosphate buffered saline (0.9%) media without adding the lecithin substrate of the phospholipase enzyme. Haemolysis occurred at all tested diluted concentrations of His-tag-VpC-terminal domain (p < 0.05), providing evidence for the independent haemolytic activity of the His-tag-VpC-terminal domain. The content of 100 μg of the His-tag-VpC-terminal domain brought the highest haemolytic activity of 80% compared to that in the three remaining contents. Significantly, the His-tag-VpC-terminal domain demonstrated not to involve the phospholipase activity in Luria-Bertani agar supplemented with 1% (vol/vol) egg yolk emulsion. All results proved the vital responsibility of the His-tag-VpC-terminal domain in causing the haemolytic activity without the required activation by the phospholipase enzyme. Raw extracts of Phellinus igniarus and Phellinus pipi at 10–1 mg/mL inhibited the haemolytic activity of the His-tag-VpC-terminal domain from 67.7% to 87.42%, respectively. Hence applying the His-tag-VpC-terminal domain as a simple biological material to evaluate quickly potential derivatives against the Vptlh in vivo conditions will accessible and more advantageous than using the whole of the Vptlh.