http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kim, Suhee,Ahn, Sun Hee,Yang, Hee-Young,Lee, Jin-Sil,Choi, Hyang-Gi,Park, Young-Kyu,Lee, Tae-Hoon Informa UK (TaylorFrancis) 2017 Redox report Vol.22 No.6
<P>Discussion: Pg appears to be redox-sensitive in cancer, and the C457 modification may impair cell migration and proliferation by affecting its interaction with the E-cadherin/catenin axis. Our findings suggest that redox-sensitive cysteines of Pg may be the targets for CRC therapy.</P>
Kim, Suhee,Park, Jeong Won,Wark, Alastair W.,Jhung, Sung Hwa,Lee, Hye Jin American Chemical Society 2017 ANALYTICAL CHEMISTRY - Vol.89 No.22
<P>The multiplexed detection of protein biomarkers in plasma present over a range of clinically relevant concentrations continues to be difficult for surface-based bioaffinity detection platforms such as surface plasmon resonance (SPR). As well as nonspecific adsorption, challenges include quantitative comparison between targets whose concentrations differ by orders of magnitude, regenerating SPR chips after plasma exposure, and the two- or four-channel limitation of many commercial SPR instruments limiting sample throughput. In this article, we explore an approach where two protein biomarkers alpha-1 antitrypsin (AAT) and Tau 381 are detected in tandem within a single SPR channel at micromolar and femtomolar concentrations, respectively. This was achieved by creating a mixed antibody (antiAAT and antiTau) monolayer on the chip surface. After the adsorption of AAT and/or Tau, further specificity was obtained via the adsorption of a DNA aptamer specific to each target. The detection range for each target was controlled via the relative surface density ratio of each antibody type as well as each aptamer concentration. Calibration measurements were performed in both buffer and spiked plasma with the detection of native concentrations of ∼39 fM (Tau) and ∼65 μM (AAT) in a human plasma sample. Finally, tandem measurements of both targets within the same SPR signal channel were demonstrated at these very different concentrations.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2017/ancham.2017.89.issue-22/acs.analchem.7b03837/production/images/medium/ac-2017-03837q_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac7b03837'>ACS Electronic Supporting Info</A></P>
KIM, Young-Ok,HONG, Suhee,NAM, Bo-Hye,LEE, Jeong-Ho,KIM, Kyung-Kil,LEE, Sang-Jun Japan Society for Bioscience, Biotechnology, and A 2005 Bioscience, Biotechnology, and Biochemistry Vol.69 No.7
<P>Hepcidin is a cysteine-rich cationic antimicrobial peptide central to iron metabolism. We report a comparative analysis of the sequences, gene organization and expression of two hepcidin genes from olive flounder <I>Paralichthys olivaceus</I>. Both consist of two introns and three exons that encode a prepropeptide (81 amino acids for hepcidin I and 89 amino acids for hepcidin II). A TATA box and several consensus-binding motifs for transcription factors were found upstream of the transcriptional starting site. Hepcidin II was predominantly expressed in the liver and highly inducible under the effect of lipopolysaccharide (LPS), while a large amount of hepcidin I transcripts was detected in various tissues but did not appear to have a significant effect during LPS-stimulation.</P>
Synthesis of 4<i>H</i>-Cyclopenta[<i>def</i>]phenanthrene from 1-Naphthylacetic Acid
Kim, Jinwoo,Jin, Youngeup,Song, Suhee,Kim, Il,Suh, Hongsuk The Chemical Society of Japan 2009 Chemistry letters Vol.38 No.10
<P>4<I>H</I>-cyclopenta[<I>def</I>]phenanthrene (CPP) was prepared from 1-naphthylacetic acid in six steps with an overall yield of 36%. From easily available ethyl 1-naphthaleneacetate, the Michael addition and Lewis acid catalyzed dicyclization provided the diketone, which was reduced and dehydrated to give CPP.</P>
Kim, Ki Woong,Kim, Soo Min,Choi, Suhee,Kim, Jongwon,Lee, In Su American Chemical Society 2012 ACS NANO Vol.6 No.6
<P>A novel electroless Pt deposition method was exploited by employing the galvanic replacement process occurring between the Mn<SUB>3</SUB>O<SUB>4</SUB> surface and PtCl<SUB>4</SUB><SUP>2–</SUP> complexes. The newly discovered process provides a simple protocol to produce the catalytic nanocomposite, in which a high density of ultrafine Pt nanocrystals is stably immobilized in a homogeneously dispersive state on the surface of Mn<SUB>3</SUB>O<SUB>4</SUB> nanoparticles. When the eletrocatalytic activity was tested for the oxygen reduction reaction, which limits the rate of the overall process in proton-exchange membrane fuel cells, the resulting Pt/Mn<SUB>3</SUB>O<SUB>4</SUB> nanocomposite showed highly enhanced specific activity and durability, compared with those of the commercial Pt/C catalyst.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2012/ancac3.2012.6.issue-6/nn300782m/production/images/medium/nn-2012-00782m_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn300782m'>ACS Electronic Supporting Info</A></P>
Kim, Suhee,Lee, Sanghyuk,Lee, Hye Jin Elsevier 2018 Sensors and actuators. B Chemical Vol.273 No.-
<P><B>Abstract</B></P> <P>A Gold nanorod (NR) enhanced surface sandwich assay utilizing a novel pair of aptamers for the attomolar concentration of norovirus (NoV) capsid protein was developed in conjunction with surface plasmon resonance (SPR). A total of four different DNA aptamer sequences (aptamer I-IV) known to be specific for the NoV protein were examined using SPR for individual binding strength with the NoV protein and for the formation of surface sandwich with the NoV protein, meaning that the chosen aptamer pair possesses different binding epitope towards the NoV protein. One of the aptamer (aptamer II) sequences with the strongest binding constant was covalently tethered onto a chemically modified thin gold chip surface, while the other aptamer I was used for tethering onto the Au NR surface. The surface sandwich complex was formed via the sequential adsorption of NoV capsid protein and Au NR coated aptamer I onto the aptamer II surface. As low as a 70 aM concentration of the NoV protein in buffer solution could be detected, which is 10<SUP>5</SUP> times better than that of using the aptamer-aptamer sandwich platform without any gold NR particles. As a demonstration, the aptamer-NR coated aptamer sandwich assay was applied to analyze NoV capsid protein concentrations spiked in human serum solutions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Highly sensitive and selective surface sandwich bioassays for norovirus (NoV) capsid protein. </LI> <LI> A pair of DNA aptamer bioreceptors for enhancing the selectivity of NoV capsid protein sensing. </LI> <LI> Gold nanorod-DNA aptamer conjugates to improve the sensitivity for NoV capsid protein sensing. </LI> <LI> Direct analysis of NoV capsid protein concentrations in undiluted human serum samples. </LI> <LI> A lowest detectable concentration of 50 aM NoV capsid protein. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Kim, Suhee,Kim, Hyunwoo,Lee, Soo-Hyeon,Cho, Ilhan,Kang, Seongwoo,Bae, Junwoo,Kim, Woosun,Ahn, Soomin,Choi, Jihye,Kim, Sang-Ki,Do, Yoonjung,Yoo, Jae Gyu,Park, Jinho,Yu, DoHyeon 한국조명·전기설비학회 2017 한국조명·전기설비학회 학술대회논문집 Vol. No.
<P>Acute lymphocytic leukemia (ALL) is uncommon lymphoid malignancy in dogs, and its diagnosis is challenging. A 14-year-old spayed female mixed breed dog was transferred to a veterinary medical teaching hospital for an immediate blood transfusion. The dog showed lethargy, pale mucous membranes, and a weak femoral pulse. Complete blood count revealed non-regenerative anemia and severe leukopenia with thrombocytopenia. ALL was tentatively diagnosed based on the predominance of immature lymphoblasts on blood film examination. For confirmation of lymphoid malignancy, PCR for antigen receptor rearrangement (PARR) on a peripheral blood sample and flow cytometry analysis were performed after blood transfusion. Flow cytometry analysis revealed that lymphocyte subsets were of normal composition, but PARR detected a T-cell malignancy. The dog was diagnosed with ALL and survived 1 wk after diagnosis. In conclusion, after blood transfusion, flow cytometry was not a reliable diagnostic method for an ALL dog, whereas PARR could detect lymphoid malignancy. Our results suggest that PARR should be the first-line diagnostic tool to detect canine lymphoid malignancy after a blood transfusion.</P>