A Design Method for Cascades Consisting of Circular Arc Blades with Constant Thickness

Bian, Tao,Han, Qianpeng,Bohle, Martin Korean Society for Fluid machinery 2017 International journal of fluid machinery and syste Vol.10 No.1

Many axial fans have circular arc blades with constant thickness. It is still a challenging task to calculate their performance, i.e. to predict how large their pressure rise and pressure losses are. For this task a need for cascade data exists. Therefore, the designer needs a method which works quickly for design purposes. In the present contribution a design method for such cascades consisting of circular arc blades with constant thickness is described. It is based on a singularity method which is combined with a CFD-data-based flow loss model. The flow loss model uses CFD-data to predict the total pressure losses. An interpolation method for the CFD-data are applied and described in detail. Data of measurements are used to validate the CFD-data and parameter variations are conducted. The parameter variations include the variation of the camber angle, pitch chord ratio and the Reynolds number. Additionally, flow patterns of two dimensional cascades consisting of circular arc blades with constant thickness are shown.

A Design Method for Cascades Consisting of Circular Arc Blades with Constant Thickness

Tao Bian,Qianpeng Han,Martin Bohle 한국유체기계학회 2017 International journal of fluid machinery and syste Vol.10 No.1

Many axial fans have circular arc blades with constant thickness. It is still a challenging task to calculate their performance, i.e. to predict how large their pressure rise and pressure losses are. For this task a need for cascade data exists. Therefore, the designer needs a method which works quickly for design purposes. In the present contribution a design method for such cascades consisting of circular arc blades with constant thickness is described. It is based on a singularity method which is combined with a CFD-data-based flow loss model. The flow loss model uses CFD-data to predict the total pressure losses. An interpolation method for the CFD-data are applied and described in detail. Data of measurements are used to validate the CFD-data and parameter variations are conducted. The parameter variations include the variation of the camber angle, pitch chord ratio and the Reynolds number. Additionally, flow patterns of two dimensional cascades consisting of circular arc blades with constant thickness are shown.

Haemaphysalis concinna (Acari: Ixodida): Persistent efficacy of doramectin in rabbits under laboratory conditions

Yao BIAN,Guangyou YANG,Tao WANG,Huijuan YAN,Shuai WANG,Jiagang SUN,Kaijun LI 한국곤충학회 2009 Entomological Research Vol.39 No.2

In the present study, the persistent efficacy of doramectin (DOR) in rabbits infested by Haemaphysalis concinna was observed. DOR (200 µg/kg) was administered once to infested rabbits by subcutaneous injection. A total of 15 rabbits were allocated to three groups, which were observed for efficacy. On days 1–7, 8–14, 15–21 and 22–28 post-inoculation, the percent reduction of tick larvae in DOR treated rabbits was 100.0, 100.0, 86.7 and 61.7%, respectively; the percent reduction of nymphs was 100.0, 90.0, 75.0 and 65.0%, respectively; and the percent reduction of adults was 85.0, 65.0, 55.0 and 55.0%, respectively. In comparison, in untreated rabbits at the same time points (control group), the percent reduction of larvae was 5.0, 3.3, 5.0 and 5.0%, respectively, and there was no reduction of nymphs and adults in the untreated rabbits.

BMB Reports : Poly(ADP-ribose) protects vascular smooth muscle cells from oxidative DNA damage

( Chao Zhang ),( Tao Luo ),( Shijun Cui ),( Yongquan Gu ),( Chunjing Bian ),( Yibin Chen ),( Xiaochun Yu ),( Zhonggao Wang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.6

Vascular smooth muscle cells (VSMCs) undergo death during atherosclerosis, a widespread cardiovascular disease. Recent studies suggest that oxidative damage occurs in VSMCs and induces atherosclerosis. Here, we analyzed oxidative damage repair in VSMCs and found that VSMCs are hypersensitive to oxidative damage. Further analysis showed that oxidative damage repair in VSMCs is suppressed by a low level of poly (ADP-ribosyl)ation (PARylation), a key post-translational modification in oxidative damage repair. The low level of PARylation is not caused by the lack of PARP-1, the major poly(ADP-ribose) polymerase activated by oxidative damage. Instead, the expression of poly(ADP-ribose) glycohydrolase, PARG, the enzyme hydrolyzing poly(ADP-ribose), is significantly higher in VSMCs than that in the control cells. Using PARG inhibitor to suppress PARG activity facilitates oxidative damage-induced PARylation as well as DNA damage repair. Thus, our study demonstrates a novel molecular mechanism for oxidative damage-induced VSMCs death. This study also identifies the use of PARG inhibitors as a potential treatment for atherosclerosis. [BMB Reports 2015; 48(6): 354-359]

Gap junction blockage promotes cadmium-induced apoptosis in BRL 3A derived from Buffalo rat liver cells

Di Hu,Hui Zou,Tao Han,Junze Xie,Nannan Dai,Liling Zhuo,Jianhong Gu,Jianchun Bian,Yan Yuan,Xuezhong Liu,Zongping Liu 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.1

Gap junctions mediate direct communication between cells; however, toxicological cascade triggered by nonessential metals can abrogate cellular signaling mediated by gap junctions. Although cadmium (Cd) is known to induce apoptosis in organs and tissues, the mechanisms that underlie gap junction activity in Cd-induced apoptosis in BRL 3A rat liver cells has yet to be established. In the present study, we showed that Cd treatment decreased the cell index (a measure of cellular electrical impedance) in BRL 3A cells. Mechanistically, we found that Cd exposure decreased expression of connexin 43 (Cx43), increased expression of p-Cx43 and elevated intracellular free Ca2+ concentration, corresponding to a decrease in gap junctional intercellular communication. Gap junction blockage pretreatment with 18b-glycyrrhizic acid (GA) promoted Cd-induced apoptosis, involving changes in expression of Bax, Bcl-2, caspase-3 and the mitochondrial transmembrane electrical potential (Δym). Additionally, GA was found to enhance ERK and p38 activation during Cd-induced activation of mitogen-activated protein kinases, but had no significant effect on JNK activation. Our results indicated the apoptosis-related proteins and the ERK and p38 signaling pathways may participate in gap junction blockage promoting Cd-induced apoptosis in BRL 3A cells.

Znf45l affects primitive hematopoiesis by regulating transforming growth factor-β signaling

Hui Juan Chen,Hua Qin Sun,Da Chang Tao,Ping Yang,Sha Sha Bian,Yun Qiang Liu,Si Zhong Zhang,Yong Xin Ma 생화학분자생물학회 2014 BMB Reports Vol.47 No.1

Dissecting the meteorological and genetic factors affecting rice grain quality in Northeast China

Chen Mojun,Li Zhao,Huang Jie,Yan Yongfeng,Wu Tao,Bian Mingdi,Zhou Jinsong,Wang Yongjun,Lyv Yanjie,Hu Guanghui,Jin Yong-Mei,Huang Kai,Guo Liping,Jiang Wenzhu,Du Xinglin 한국유전학회 2021 Genes & Genomics Vol.43 No.8

Background The Northeast Plain of China, which is an important region for the production of high grain quality rice in China. However, the grain quality of the rice produced varies across this region, even for the same cultivar. Objective In order to explore the meteorological factors that have the greatest infuence on quality and the transcriptional level diferences between diferent cultivars and diferent locations at grain flling stage. Methods We grew eight rice cultivars in three locations in Northeast China during two growing seasons (2017 and 2018). We recorded meteorological conditions, including air temperature, air temperature range, and photosynthetically active radiation (PAR) during the grain-flling stage of each cultivar, and analyzed the grain quality of those eight cultivars. Results Across all eight cultivars, meteorological factors had a stronger efect on eating quality than genotype, while genotype had a stronger efect on milling quality. Of the three environmental factors assessed, PAR was signifcantly correlated with the most grain quality traits. Using RNA-sequencing analysis, we identifed 573 environment-specifc DEGs (Diferentially Expressed Genes), and 119 genotype-specifc DEGs; 11 DEGs were responsive to genotype×environment interactions. These DEGs were involved in many key metabolic processes. Conclusion Our results indicated that interactions among environmental factors, especially PAR, afected rice quality in Northeast China. Further analyses of the DEGs identifed herein may provide useful information for future breeding programs aiming to develop high grain quality rice varieties suitable for cultivation across Northeast China.

De novo transcriptome sequencing and gene expression profiling of sweetpotato leaves during low temperature stress

Pan Jiaquan,Zhang Qian,Liu Shuai,Liu Zhiqiang,Bian Xiaofeng,Yu Tao 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.6

Sweetpotato [Ipomoea batatas (L.) Lam] is one of the most important crops for food, feed, industrial material, and bio-energy resource. However, since it originated in tropical regions, sweetpotato exhibits limited cold tolerance, hindering its cultivation in low-temperature areas. To comprehend the sweetpotato response to chilling stress, de novo transcriptome assembly was conducted on leaves from two sweetpotato cultivars: the cold-tolerant Liaohanshu 21 and the cold-sensitive Shenshu 28, exposed to low-temperature stress for 3 h and 24 h. 787.24 million raw reads and 763.44 million clean reads were obtained, 60.60–66.06% of clean reads successfully mapped to the reference database across all 18 libraries. 2524 unigenes were identified as transcript factors and classified into 55 families in Plant Transcription Factor Database (PlantTFDB) and 4671 unigenes were matched to 24 groups in Plant Resistance Gene Database (PRGdb). In Liaohanshu 21, 3618 genes and 8391 genes exhibited significant differential expression 3 h and 24 h of low-temperature exposure, respectively. In Shenshu 28, 13,394 genes and 11,627 genes displayed substantial differential expression after 3 h and 24 h of low-temperature exposure, respectively. The differentially expressed genes (DEGs) were further analyzed using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genome (KEGG) database. DEGs related to “active oxygen scavenging systems”, “lipid metabolism”, and “plant hormone signal transduction” were potentially associated with sweetpotato’s cold resistance. This research offers insights into the mechanisms and genes involved in cold stress in sweetpotato’s response to cold stress and will contribute valuable information for cold-tolerant breeding of sweetpotato.