Molecular Phylogenetic Diversity and Spatial Distribution of Bacterial Communities in Cooling Stage during Swine Manure Composting

Yan Guo,JInliang Zhang,Yongfeng Yan,Jian Wu,Nengwu Zhu,Changyan Deng 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.6

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and subsequent sub-cloning and sequencing were used in this study to analyze the molecular phylogenetic diversity and spatial distribution of bacterial communities in different spatial locations during the cooling stage of composted swine manure. Total microbial DNA was extracted, and bacterial near full-length 16S rRNA genes were subsequently amplified, cloned, RFLP-screened, and sequenced. A total of 420 positive clones were classified by RFLP and near-full-length 16S rDNA sequences. Approximately 48 operational taxonomic units (OTUs) were found among 139 positive clones from the superstratum sample; 26 among 149 were from the middle-level sample and 35 among 132 were from the substrate sample. Thermobifida fusca was common in the superstratum layer of the pile. Some Bacillus spp. were remarkable in the middle-level layer, and Clostridium sp. was dominant in the substrate layer. Among 109 OTUs, 99 displayed homology with those in the GenBank database. Ten OTUs were not closely related to any known species. The superstratum sample had the highest microbial diversity, and different and distinct bacterial communities were detected in the three different layers. This study demonstrated the spatial characteristics of the microbial community distribution in the cooling stage of swine manure compost.

Molecular Phylogenetic Diversity and Spatial Distribution of Bacterial Communities in Cooling Stage during Swine Manure Composting

Guo, Yan,Zhang, Jinliang,Yan, Yongfeng,Wu, Jian,Zhu, Nengwu,Deng, Changyan Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.6

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and subsequent sub-cloning and sequencing were used in this study to analyze the molecular phylogenetic diversity and spatial distribution of bacterial communities in different spatial locations during the cooling stage of composted swine manure. Total microbial DNA was extracted, and bacterial near full-length 16S rRNA genes were subsequently amplified, cloned, RFLP-screened, and sequenced. A total of 420 positive clones were classified by RFLP and near-full-length 16S rDNA sequences. Approximately 48 operational taxonomic units (OTUs) were found among 139 positive clones from the superstratum sample; 26 among 149 were from the middle-level sample and 35 among 132 were from the substrate sample. Thermobifida fusca was common in the superstratum layer of the pile. Some Bacillus spp. were remarkable in the middle-level layer, and Clostridium sp. was dominant in the substrate layer. Among 109 OTUs, 99 displayed homology with those in the GenBank database. Ten OTUs were not closely related to any known species. The superstratum sample had the highest microbial diversity, and different and distinct bacterial communities were detected in the three different layers. This study demonstrated the spatial characteristics of the microbial community distribution in the cooling stage of swine manure compost.

An Improved Saliency Detection for Different Light Conditions

( Yongfeng Ren ),( Jingbo Zhou ),( Zhijian Wang ),( Yunyang Yan ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.3

In this paper, we propose a novel saliency detection framework based on illumination invariant features to improve the accuracy of the saliency detection under the different light conditions. The proposed algorithm is divided into three steps. First, we extract the illuminant invariant features to reduce the effect of the illumination based on the local sensitive histograms. Second, a preliminary saliency map is obtained in the CIE Lab color space. Last, we use the region growing method to fuse the illuminant invariant features and the preliminary saliency map into a new framework. In addition, we integrate the information of spatial distinctness since the saliency objects are usually compact. The experiments on the benchmark dataset show that the proposed saliency detection framework outperforms the state-of-the-art algorithms in terms of different illuminants in the images.

Is National Culture an Appropriate Unit of Analysis in Cross-Cultural Research?

Yongfeng Zhang(장영봉),Jongwook Kwon(권종욱),Yan Zhou(주암),Peng Wang(왕붕) 연세대학교 경영연구소 2021 연세경영연구 Vol.58 No.3

본 연구는 다문화 연구 분야에서 논란이 되고 있는 구체적인 연구문제를 해결하는 것이 목적이다. 다문화 연구에 있어서 연구의 분석단위가 국가인지 아니면 한 국가내의 지역인지가 논쟁이 되고 있다. 수십 년 동안 다양한 분야의 연구자들이 이러한 이론적 논쟁에 매달려 오고 있다. 본 연구는 Minkov and Hofstede(2012)의 연구 방법과 세계 가치 조사(World Values Survey)의 최신 데이터를 활용하여 비교문화 연구에서 분석의 단위가 국가가 바람직한지 아니면 지역단위가 바람직한지를 검증해 보고자 한다. 이를 위해 슈와르츠 가치관 값을 활용하여 5개 그룹, 19개국 239개 지역의 문화적 가치 차이를 분석한다. 5개 그룹은 아시아, 라틴 아메리카, 동유럽, 아랍 세계, 그리고 영국이 포함된다. 분석결과 국가간 문화차이보다 국가 내 문화적 차이가 큰 경우도 있고(중국·페루·러시아 등) 작은 경우(미국·요르단 등)도 발견되었다. 하지만 전체적으로 국가간 차이보다 지역간 차이가 작아서 비교문화 연구에서 연구의 분석단위는 한 국가 내 지역보다는 국가단위가 바람직한 것으로 나타났다. 또한 시간이 지날수록 국가 클러스터가 모이는 패턴이 달라진다는 사실도 발견했다. 본 연구는 비교문화 연구에서 논쟁이 되고 있는 국가 및 지역 단위의 분석단위 적합성에 대한 이론적 기반을 제공하고 다국적 기업들 간의 시장 전략 수립에 유용할 정보를 제공한다. The purpose of this paper is to address a specific controversial issue in the cross-cultural field: in cross-cultural studies, regarding whether the country or the region should be used as the unit of analysis. Cross-cultural similarities and differences play an important role in many areas of life. Researchers from various fields have been actively dealing with this topic for decades. Based on the research methods of Minkov and Hofstede (2012), and using the latest data from the World Values Survey, based on the Schwartz values, this study analyzes the differences in cultural values among 239 regions of 19 countries taken from five groups: Asia, Latin America, Eastern Europe, the Arab World, and Anglo countries. By analyzing the aggregation of homogeneous and heterogeneous clusters, this paper examines the judgment basis for and use of analytical units in cross-cultural research. The results show that cultural differences within some countries can be larger (e.g, China, Peru, Russia, etc.) or smaller (e.g, United States, Jordan, etc.) than differences among countries. We have also found a pattern of national cluster aggregation will be changed over time. This research provides a reference basis for effectively distinguishing the types of differences in national and regional values and provides theoretical information that will be useful in the formulation of market strategies among multinational companies.

The new focus of energy storage: flexible wearable supercapacitors

Yani Yan,Yi Zhou,Yongfeng Li,Yanzhen Liu 한국탄소학회 2023 Carbon Letters Vol.33 No.6

As the demand for flexible wearable electronic devices increases, the development of light, thin and flexible high-performance energy-storage devices to power them is a research priority. This review highlights the latest research advances in flexible wearable supercapacitors, covering functional classifications such as stretchability, permeability, self-healing and shapememory capabilities, as well as practical studies on energy harvesting capabilities.

Asymptotic Synchronization Control of High-order Nonlinear Multiagent Systems Using Barrier Functions

Jiacheng Song,Yongfeng Ju,Maode Yan,Panpan Yang 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.4

This paper studies the adaptive control problem for unknown time-varying high-order nonlinear multiagent systems (MASs) with directed topology. A novel low-complexity distributed adaptive backstepping controller is developed to achieve the asymptotic synchronization. The designed controller regards the unknown system nonlinearities as “disturbance-like” terms, which are guaranteed to be bounded by using the barrier functions, such that detail models of system nonlinearities are released. Then, the “disturbance-like” terms are compensated adaptively by designing the novel compensator at each step, such that the synchronization error is eliminated to zero eventually for each agent. In addition, unlike the existing backstepping-like synchronization approaches for high-order nonlinear MASs, the “explosion of complexity” issue is avoided without extra low-pass filters. Some simulations are shown to demonstrate the effectiveness and advantages of the developed method.

Inhibition of acetylation of histones 3 and 4 attenuates aortic valve calcification

Jia Gu,Yan Lu,Menqing Deng,Ming Qiu,Yunfan Tian,Yue Ji,Pengyu Zong,Yongfeng Shao,Rui Zheng,Bin Zhou,Xiangqing Kong,Wei Sun 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Aortic valve calcification develops in patients with chronic kidney disease who have calcium and phosphate metabolic disorders and poor prognoses. There is no effective treatment except valve replacement. However, metabolic disorders put patients at high risk for surgery. Increased acetylation of histones 3 and 4 is present in interstitial cells from human calcific aortic valves, but whether it is involved in aortic valve calcification has not been studied. In this study, we found that treating cultured porcine aortic valve interstitial cells with a high-calcium/high-phosphate medium induced calcium deposition, apoptosis, and expression of osteogenic marker genes, producing a phenotype resembling valve calcification in vivo. These phenotypic changes were attenuated by the histone acetyltransferase inhibitor C646. C646 treatment increased the levels of class I histone deacetylase members and decreased the acetylation of histones 3 and 4 induced by the high-calcium/high-phosphate treatment. Conversely, the histone deacetylase inhibitor suberoylanilide hydroxamic acid promoted valve interstitial cell calcification. In a mouse model of aortic valve calcification induced by adenine and vitamin D treatment, the levels of acetylated histones 3 and 4 were increased in the calcified aortic valves. Treatment of the models with C646 attenuated aortic valve calcification by restoring the levels of acetylated histones 3 and 4. These observations suggest that increased acetylation of histones 3 and 4 is part of the pathogenesis of aortic valve calcification associated with calcium and phosphate metabolic disorders. Targeting acetylated histones 3 and 4 may be a potential therapy for inoperable aortic valve calcification in chronic kidney disease patients.

Recycling of Sintered Nd-Fe-B Magnets Doped with PrNd Nanoparticles

Xuefeng Zhang,Fei Liu,Yanli Liu,Qiang Ma,Yongfeng Li,Qian Zhao,Gaofeng Wang,Zhubai Li 한국자기학회 2015 Journal of Magnetics Vol.20 No.2

The waste of sintered Nd-Fe-B magnets was recycled using the method of dopingPrNd nanoparticles. The effect of PrNd nanoparticle doping on the magnetic properties of the regenerated magnets has been studied. As the content of the PrNd nanoparticles increases, the coercivity increases monotonically, whereas both the remanence and the maximum energy products reach the maximum values for 4 wt% PrNd doping. Microstructural observation reveals that the appropriate addition of PrNd nanoparticles improves the magnetic properties and refines the grain. Domain investigation shows that the self-pinning effect of the rare earth (Re)-rich phase is enhanced by PrNd nano-particle doping. Compared to the magnet with 4 wt% PrNd alloy prepared using the dual-alloy method, the regenerated magnet doped with the same number of PrNd nanoparticles exhibits better magnetic properties and a more homogeneous microstructure. Therefore, it is concluded that PrNd nanoparticle doping is an efficient method for recycling the leftover scraps of Nd-Fe-B magnets.

Characterization of high-alumina coal fly ash based silicate material and its adsorption performance on volatile organic compound elimination

Guojun Yuan,Jianbin Zhang,Yongfeng Zhang,Yinan Yan,Xinxin Ju,Junmin Sun 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.3

A highly stable silicate material from high-alumina coal fly ash was prepared and characterized using X-raydiffraction, scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, thermal analysis, Xrayphotoelectron spectroscopy, and elemental analysis. The spectral results show that the silicate material was mainlycomposed of six elements, C, Ca, O, Si, Mg, and Al, in the form of Ca2+, Mg2+, Al3+, SiO32−, and CO32− ions. Some adsorbedwater and/or water of crystallization was also observed. The silicate material showed exceptionally high capabilityto adsorb volatile organic compounds (VOCs). The results of dynamic adsorption behavior show that the silicatematerial presents similar properties with commercial activated carbon and stronger absorption properties than commercialdiatomite for the adsorption of VOCs. The FTIR spectral results show weak hydrogen bonding interactions ofthe silicate material with three VOCs.

Dissecting the meteorological and genetic factors affecting rice grain quality in Northeast China

Chen Mojun,Li Zhao,Huang Jie,Yan Yongfeng,Wu Tao,Bian Mingdi,Zhou Jinsong,Wang Yongjun,Lyv Yanjie,Hu Guanghui,Jin Yong-Mei,Huang Kai,Guo Liping,Jiang Wenzhu,Du Xinglin 한국유전학회 2021 Genes & Genomics Vol.43 No.8

Background The Northeast Plain of China, which is an important region for the production of high grain quality rice in China. However, the grain quality of the rice produced varies across this region, even for the same cultivar. Objective In order to explore the meteorological factors that have the greatest infuence on quality and the transcriptional level diferences between diferent cultivars and diferent locations at grain flling stage. Methods We grew eight rice cultivars in three locations in Northeast China during two growing seasons (2017 and 2018). We recorded meteorological conditions, including air temperature, air temperature range, and photosynthetically active radiation (PAR) during the grain-flling stage of each cultivar, and analyzed the grain quality of those eight cultivars. Results Across all eight cultivars, meteorological factors had a stronger efect on eating quality than genotype, while genotype had a stronger efect on milling quality. Of the three environmental factors assessed, PAR was signifcantly correlated with the most grain quality traits. Using RNA-sequencing analysis, we identifed 573 environment-specifc DEGs (Diferentially Expressed Genes), and 119 genotype-specifc DEGs; 11 DEGs were responsive to genotype×environment interactions. These DEGs were involved in many key metabolic processes. Conclusion Our results indicated that interactions among environmental factors, especially PAR, afected rice quality in Northeast China. Further analyses of the DEGs identifed herein may provide useful information for future breeding programs aiming to develop high grain quality rice varieties suitable for cultivation across Northeast China.