http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Taniguchi, Junichi,Pandian, Ganesh N.,Hidaka, Takuya,Hashiya, Kaori,Bando, Toshikazu,Kim, Kyeong Kyu,Sugiyama, Hiroshi Oxford University Press 2017 Nucleic acids research Vol.45 No.16
<P><B>Abstract</B></P><P>Targeted differentiation of human induced pluripotent stem cells (hiPSCs) using only chemicals would have value-added clinical potential in the regeneration of complex cell types including cardiomyocytes. Despite the availability of several chemical inhibitors targeting proteins involved in signaling pathways, no bioactive synthetic DNA-binding inhibitors, targeting key cell fate-controlling genes such as SOX2, are yet available. Here, we demonstrate a novel DNA-based chemical approach to guide the differentiation of hiPSCs using pyrrole–imidazole polyamides (PIPs), which are sequence-selective DNA-binding synthetic molecules. Harnessing knowledge about key transcriptional changes during the induction of cardiomyocyte, we developed a DNA-binding inhibitor termed <B>PIP-S2</B>, targeting the 5′-CTTTGTT-3′ and demonstrated that inhibition of SOX2–DNA interaction by <B>PIP-S2</B> triggers the mesoderm induction in hiPSCs. Genome-wide gene expression analyses revealed that <B>PIP-S2</B> induced mesoderm by targeted alterations in SOX2-associated gene regulatory networks. Also, employment of <B>PIP-S2</B> along with a Wnt/β-catenin inhibitor successfully generated spontaneously contracting cardiomyocytes, validating our concept that DNA-binding inhibitors could drive the directed differentiation of hiPSCs. Because PIPs can be fine-tuned to target specific DNA sequences, our DNA-based approach could be expanded to target and regulate key transcription factors specifically associated with desired cell types.</P>
Regeneration of Plantlets from petiole callus of wild viola (Viola patrinii DC.)
Miyake,Hiroshi,Taniguchi,Takeshi,Sato,Tadahiko,Oh,Chang Kwon,Maeda,Eizo 東亞大學校附設遺傳工學硏究所 1996 遺傳工學硏究 Vol.- No.3
Plantlets were regenerated from 5-year subcultured compact callus derived from petiole tissues of wild viola (Viola patrinii DC.) but not from 5-year subcultured friable callus. Regeneration occurred most efficiently on medium that contained two-fold diluted basal salts of Murashige and Skoog's (MS) medium, 5×10-6M 1-naphthaleneacetic acid and ?? M kinetin. The effect of dilution of MS basal salts could also be achieved solely by two-fold dilution of the potassium dihydrogen phosphate in the mixture. The present study revealed that dilution of MS basal salts, in particular of potassium dihydrogen phosphate, was important for the regeneration of wild viola. Moreover, although the callus had been subcultured for 5 years, regeneration of plantlets from callus was still possible. In addition, scanning electron microscopy revealed that details of the process of plant regeneration from subcultured callus varied with the age and source of callus and differed from that reported in rice.
Spin Torque Assisted Magnetization Switching in Thermally Activated Region
Tomohiro Taniguchi,Hiroshi Imamura 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.62 No.12
The spin torque assisted magnetization switching in the thermally activated region was theoreticallystudied based on the rate equation. We found the dependence of the switching current on thecurrent pulse duration time to be linear for b = 1 and nonlinear for b = 2. Here, b is the exponentof the current term in the switching rate formula, which has been assumed to be unity while atheory with b = 2 was proposed recently. A comparison of the calculated results with the recentexperiments of Heindl et al. [J. Appl. Phys. 109, 073910 (2011)] indicates b 6= 1.
Nakata, Kazue,Taniguchi, Yoshie,Yoshioka, Noriko,Yoshida, Aya,Inagawa, Hiroyuki,Nakamoto, Takeru,Yoshimura, Hiroshi,Miyake, Shin-Ichiro,Kohchi, Chie,Kuroki, Masahide,Soma, Gen-Ichiro The Korean Nutrition Society 2011 Nutrition Research and Practice Vol. No.
At present, lifestyle-related diseases are one of the most critical health issues worldwide. It has been reported that lipopolysaccharide derived from a Gram-negative bacteria (IP-PA1) symbiotic with wheat exhibited several advantageous biological effects, such as the reduction of plasma glucose levels in NOD mice and low-density lipoprotein (LDL) levels in WHHL rabbits. In this study, the beneficial effects on plasma glucose and lipids of a tea (SI tea) consisting of IP-PA1 and Salacia (which contains an inhibitor of ${\alpha}$-glucosidase) were investigated in the KK-Ay/TaJcl type 2 diabetic model mice and in human subjects with premetabolic syndrome in a double-blind, randomized study. S1 tea significantly decreased plasma glucose levels in KK-Ay/TaJcl mice. A clinical trial of SI tea was performed with 41 subjects between the ages of 40 and 69, who belonged either to a high plasma glucose group (HG: FPG 100-125 mg/dl) or to a hyperlipidemia group (HL: TG ${\geq}$ 150 mg/dl, or LDL ${\geq}$ 120 mg/dl, or HDL <40 mg/dl). These subjects ingested either Salacia without IP-PA1 (the control) or SI tea. Blood samples were collected at 0, 30, and 60 days after initiating SI tea treatment, and were measured for FPG, HbA1c, TG, LDL, and HDL. These results showed that SI tea reduced FPG and HbA1c more rapidly than the control in the HL group, and also significantly improved LDL and HDL levels in the HG group. Thus, SI tea may be helpful in preventing lifestyle-related diseases.