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Bin Li,Sang-Bin Lee,Kozo Watanabe,Dong-HwanKim,Muyoung Heo,Young-Seuk Park,Tae-Soo Chon 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04
Investigating loci compositions by conventional methods is limited in fully addressing complex gene information. We applied self-organizing map (SOM) to characterize Amplified Fragment Length Polymorphism (AFLP) of aquatic insects in six streams in Japan in responding to environmental variables. Locus band presence patterns were clustered by the trained SOM. Presence and absence data of loci were altered and cluster change through recognition was Subsequently expressed to indicate sensitivity to environmental variables. The outlier loci were determined based on the 90th percentile. Subsequently environmental responsiveness was obtained for each outlier in different species. Outlier loci were overall sensitive to pollutants and feeding material. Poly-loci like responsiveness was detected in adapting to environmental constraints. SOM training combined with recognition could be an efficient means of characterizing loci information without knowledge on population genetics a prior.
Van Hien La,Bok-Rye Lee,Qian Zhang,Sang Hyun Park,Md. Tabibul Islam,Tae-HwanKim 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.1
Salicylic acid (SA) has been regarded as the important phytohormone in improving abiotic stress tolerance. However, thephysiological mechanisms on drought stress tolerance remain largely unknown. To investigate the role of SA in drought-stresstolerance in Chinese cabbage ( Brassica rapa subsp. pekinensis ) with regard to cellular redox control and proline metabolism,Chinese cabbages were pretreated or untreated with SA for 7 days, then grown under well-watered or drought-stressed conditionsfor 14 days. The osmotic potential and chlorophyll and carotenoid contents were decreased in the drought-stressedplants; however, the negative eff ects of drought on these parameters was substantially ameliorated in the SA-pretreatedplants. Drought stress resulted in increased O 2− , H 2 O 2 , and malondialdehyde (MDA) contents in the non-SA pretreatedplants relative to the SA-pretreated plants. Superoxide dismutase, catalase, guaiacol peroxidase, and ascorbate peroxidasewere highly activated in drought-stressed plants, whereas these substances were further activated in the SA-pretreated plantsunder drought stress. Drought stress resulted in a signifi cant decrease of the reduced/oxidized glutathione (GSH/GSSG) andreduced/oxidized nicotinamide adenine dinucleotide phosphate (NADPH/NADP) ratios, whereas plants pretreated with SAhad levels similar to those in the well-watered plants. Under drought conditions, SA pretreatment signifi cantly increasedproline content by up-regulating the expression of genes encoding pyrroline-5-carboxylate synthase ( P5CSA and P5CSB ) anddown-regulating the expression of the gene encoding proline dehydrogenase ( PDH ) compared to non-SA pretreated plants. These results indicate that a pretreatment with SA improves drought-stress tolerance by maintaining redox homeostasis andactivating proline biosynthesis.
Si-YunRyu,Bit-NaKang,Sung-whanCho,Hwa-youngSon,Kyu-ShikJeong,Sang-JoonPark,Sung-HoKim,Se-RaKim,Tae-HwanKim,Mi-youngAn,Ho-Jun Kim 대한수의학회 2002 Journal of Veterinary Science Vol.3 No.4
The detrimental effects of environmental pollutantson the health of the individual are generally accepted,although the mechanisms of these effects remain tobe incompletely understood.In the present study,weexamined the effects of B[a]P,2-BP,phenol and TCDDon proinflammatory cytokine gene expression in micespleen cells which were stimulated with anti-CD3.10-9M TCDD increased IFNγ and TNFα gene expression,but suppressed IL-1 gene expression. 10-6M phenolinhibited IL-1, IL-6 and TNFα gene expression, and10-6M of 2-BP downregulated TNFα gene expression.However, 10-6M of B[a]P did not influence on IL-1,IL-6,IFNγ and TNFα gene expression. These findingssuggest that TCDD may impair the immune functionsof mice by enhancing proinflammatory cytokines pro-duction, whereas phenol and 2-BP may impair thefunctions by inhibiting the production of thesecytokines.