Efficient Interleukin-21 Production by Optimization of Codon and Signal Peptide in Chinese Hamster Ovarian Cells

( Hee Jun Cho ),( Byung Moo Oh ),( Jong-tae Kim ),( Jeewon Lim ),( Sang Yoon Park ),( Yo Sep Hwang ),( Kyoung Eun Baek ),( Bo-yeon Kim ),( Inpyo Choi ),( Hee Gu Lee ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.2

Interleukin-21 is a common γ-chain cytokine that controls the immune responses of B cells, T cells, and natural killer cells. Targeting IL-21 to strengthen the immune system is promising for the development of vaccines as well as anti-infection and anti-tumor therapies. However, the practical application of IL-21 is limited by the high production cost. In this study, we improved IL-21 production by codon optimization and selection of appropriate signal peptide in CHO-K1 cells. Codon-optimized or non-optimized human IL-21 was stably transfected into CHO-K1 cells. IL-21 expression was 10-fold higher for codon-optimized than non-optimized IL-21. We fused five different signal peptides to codon-optimized mature IL-21 and evaluated their effect on IL-21 production. The best result (a 3-fold increase) was obtained using a signal peptide derived from human azurocidin. Furthermore, codon-optimized IL-21 containing the azurocidin signal peptide promoted IFN-γ secretion and STAT3 phosphorylation in NK-92 cells similar to codon-optimized IL-21 containing original signal peptide. Collectively, these results indicate that codon optimization and azurocidin signal peptides provide an efficient approach for the high-level production of IL-21 as a biopharmaceutical.

한글 텍스트에서의 효율적인 스트링 매칭 알고리즘에 관한 연구

조희열,양태천 慶星大學校 1995 論文集 Vol.16 No.2

In they thesis, we study the efficient string matching algorithms for hangul text. There are many the String Matching Algorithms such as the Knuth-Morris-Pratt's Algorithm, the Boyer-Moore's Algorithm, and Rabin-karp's Algorithm. However, no efficient algorithm is know in hangul text. We propose the efficient string matching algorithm in Hangul text by experiments result.

금연을 위한 5일 프로그램

조희태 昌原大學校 學生生活硏究所 1992 학생생활연구 Vol.5 No.-

노인종합복지관 의료부문 건축계획에 관한 연구 : 서울시 사례를 중심으로 focused on Cases in Seoul

조태희,박재승 한국의료복지시설학회 2005 의료·복지 건축 Vol.11 No.3

Because of the rapid increase of the number of elderly people, there are many problem in modern society. Especially the support of elderly people is becoming the hot issue, which results from a nuclear family and social activity of woman. The Policy of community care of the elderly people in western is caused by a heavy cost and isolation from the society of a facility care policy. Since 198O, Concept of 'home care for elderly people' has been appeared in korea. The Facility causing by these movement is 'Multipurpose senior center'. The purpose of this study is to present the alternative data to improve the quality of medical and rehabilitation part in multipurpose senior center. Data collecting skills such as drawing documents analysis, interviews, observation were used to examine the condition of medical and rehabilitation. And, case study method was used to generate the altenatlve medel which can be used for the spatial composition and the area calculation.

상아질에 대한 저점도 복합레진의 자가접착에 관한 연구

조태희,최경규,박상혁,박상진 大韓齒科保存學會 2003 Restorative Dentistry & Endodontics Vol.28 No.3

The objectiveness of this study was to evaluate whether low-viscosity composite can bond effectively to dentin surface without bonding resin. The low-viscosity composites being 50wt% filler content were made by the inclusion of bonding resin of two self-etching systems(Clearfil SE Bond, Unifil Bond) varied with contents as 0, 10, 20, 30, 40, 50wt%. Exposed dentin surfaces of extracted 3rd molars are used. Dentin bond strengths were measured. The tests were carried out with a micro-shear device placed testing machine at a CHS of 1mm/min after a low-viscosity composite was filled into an iris cut from micro tygon tubind with internal diameter approximately 0.8mm and height of 1.0mm. 1. Flexural strength and modulus was increased with the addition of bonding resin. 2. Micro-shear bond strength to dentin was improved according to content of bonding resin irrespective of applying or not bonding resin in bonding procedure, and that of Clearfil SE Bond groups was higher than Unifil Bond. 3. There were no significant difference whether use of each bonding resin in bonding procedure for S-40, S-50, U-50(p>0.05). 4. In SEM examination, resin was well infiltrated into dentin after primed with self-etching primer only for S-50 and U-50 in spite of the formation of thinner hybrid layer. Low viscosity composite including some functional monomer may be used as dentin bonding resin without an intermediary bonding agent. It makes a simplified bonding procedure and foresees the possibility of self-adhesive restorative material.

필러의 실란처리농도가 복합레진의 특성에 미치는 영향

조태희,박상진 대한치과보존학회 2001 Restorative Dentistry & Endodontics Vol.26 No.1

The purpose of this study was to search the optimal silane concentrations for filler- silanization of seven experimental composites. Silica filler was a 25 micron crushed type. 0.0%, 0.5%, 1.0%, 1.5%, 2.0%, and 3.0% silane(r-methacrylooxypropyltrimethoxysilane) were added into silica-filler with weight percentage(wt%). Mixtures (silica filler/silane)were reacted at 60℃ for 72hours, and crushed into fine particles those were used as fillers for 7 experimental composites. Monomer was a 3:1 mixture of Bis-GMA and TEGDMA containing 0.2% tertiary amine and 0.4% camphoroquinone for light curability. A ratio for mixing the monomer and filler was 75% and 25% respectively. Seven experimental composites was classified with the concentration of silane treated, and the specimen number for each test was 10. Specimens with 6mm diameter and 3mm height dimension for measuring the diametral tensile strength were destroyed with 1mm/min cross-head speed on Instron universal testing machine (No. 4467, USA). Shear bond strength was measured on the specimens bonded to bovine enamel etched with 37% phosphoric acid solution for 1 minute. Fractured surfaces were observed by SEM (Hitachi S-3200, Japan) among that of the highest values measured from each groups. Following results were obtained: 1.Experimental compositeds containing silanized filler showed the significantly higher diametral tensile strength and shear bond strength than the composites containing un-silanized fillers(Group1) (p<0.05). 2.In silanized filler composite resins(Group 2~7), Diametral tensile strength of Group 3 showed the significantly higher than that of Group 2 and Group 6(p<0.05). 3.Shear bond strength was higher in Group 3 than that of Group 7 (p<0.05)in silanized fillers composite resins. 4.Fracture surface was formed in resin matrixes on the specimens from composites containing the fillers treated with 0.5%, 1.0%, and 1.5% silane. These results mean that the optimal silane concentrations are exist for each filler with its size and surface area, and that 1.0% is a optimal value for concentration to coat the 25㎛ filler with silane.

주의력결핍과잉행동장애와 Alpha-1C-Adrenergic 수용체 유전자와의 연관성에 대한 연구

조수철,김재원,김붕년,황준원,박미라,김순애,조대연,유희정,정운선,손정우,박태원 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.1

Objectives : Neurobiological and pharmacological research has suggested that the dysregulation of the central noradrenergic systems might be involved in the pathophysiology of ADHD. The aim of this study was to examine the association of the alpha-1C-adrenergic receptor gene (ADRA1C) with ADHD in Korean subjects. Methods : In a case-control study, we assessed 186 DSM-IV ADHD probands and 150 normal controls. One hundred and eight trios were studied in a family-based association analysis. Psychiatric diagnoses were derived through structured diagnostic interviews. For the clinical evaluation of the ADHD subjects, the Child Behavior Checklist (CBCL), the ADHD Rating Scale-IV (ARS) and the Junior Temperament and Character Inventory (JTCI) were administered. A computerized continuous performance test (CPT) was used to measure the inattention and impulsivity of the ADHD children. Results : There were no significant differences in the genotype or allele frequencies of the ADRA1C PstI polymorphism between the ADHD and control group (p>0.05). The transmission disequilibrium test (TDT) analysis observed no evidence for biased transmission of any of the alleles of the PstI polymorphism. There were no significant differences in the CPT or JTCI profiles between those ADHD subjects with the CC genotype and those with the other (CT+TT) genotypes at the PstI polymorphism. Conclusion : The results of this study do not support the ADRA1C as a major genetic susceptibility factor in ADHD.

임신 중 발견된 대망 종괴 1례

조인숙,김태의,김정식,이해혁,이권해,김희경,남계현 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

A case of omental mass during Pregnancy Omental cyst is a rare lesion and occur primarily during childhood and young adulthood. When encountered, most of them are benign and intraabdominal masses. It shows a large mass but not palpable and often asymptomatic. The precise diagnosis is made by most commonly at surgery or during pathologic examination. So, we report this case with brief review of literatures.

MathCAD를 사용한 X-선 회절상의 계산

조태경,송인명,권영준,이희복 공주대학교 사범대학 과학교육연구소 1994 과학교육연구 Vol.25 No.1

물질의 결정구조를 연구하는 데에는 일반적으로 X-선 회절상의 분석방법을 가장 많이 사용한다. 따라서 대학교육과정에서 고체물리학, 결정학, 재료과학 등 여러 분야에서 X-선 분광법에 대하여 공부한다. 그러나 실제 강의에서 X-선 회절의 이론을 X-선 회절상의 분석 방법과 직접 대응시켜 설명하기란 쉽지 않다. 본 논문은 MathCAD란는 software을 써서 X-선 회절 이론식으로 부터 간단하게 원하는 결정의 X-선 회절상을 계산하는 방법을 제시하고, X-선 결정구조 분석에 관한 학습과 연구에 활용할 수 있는 자료가 되도록 하였다. The most available technique for the study of the crystal structure is the analysis of X-ray diffraction patterns. For this reason lectures on X-ray diffractions have been given at universities in various subjects such as solid state physics, crystallography, material science, etc. However, it is not easy to teach the theory of X-ray diffraction in conjunction with the analysis X-ray diffraction patterns. In this study, we have used MathCAD software to calculate angles, intensities, and patterns of the X-ray diffraction of crystal using formula given in the theory without any complication. The program has shown in the study will be useful for research on crystallography as well as for teaching the subject.

한국인에서 초음파유화흡입술을 이용한 백내장수술시 diclofenac sodium의 축동 방지효과

조희태,안영 동국대학교 경주대학 1997 東國論集 Vol.16 No.1

한국인과 같이 짙은 색을 띤 홍채에 초음파유화흡입술을 이용한 백내장수술시 Diclofenac sodium의 축동방지효과를 알아보고자, 40명의 백내장환자를 각 20명씩 2군(diclofenac치료군, 대조군)으로 나누어 double blind test로 본 연구를 시행하였다. 모든 대상 환자들은 백내장수술시 일상적으로 투약되는 약을 술전에 점안했고 치료군은 0.1% diclofenac sodium을 수술 2시간전부터 30분 간격으로 국소 점안하였다. 초음파유화흡입술로 백내장을 제고하고, 추방인공수정체를 삽입한 후 측정한 수술전 후의 동공 변화는 치료군은 -1.31±0.154㎜, 대조군에서 -2.51±0.232㎜로 diclofenac sodium투여군이 술후 축동이 작게 일어나는 것으로 나타났으며, 두 군 사이에 통계학적 유의성이 있었다(p=0.001). Forty patients undergoing elective phacoemulsification with intraocular lens insertion were divided in to a control group(n=20), treated group(n=20) in a double blind, prospective, randomized clinical study to evaluated the nonsteroidal, anti inflammatory drug, diclofenac sodium chloride as an aid for maintaining pupil dilatation during surgery in darkly pigmented iris like Korean. Both groups received the routine preoperative dilating drops and the treated group also received 0.1% topical diclofenac sodium chloride. After lens remove with phacoemulsifier and posterior chamber intraocular lens insertion, the change in pupil diameter from the preoperative measurement average -1.31±0.154㎜ in treated group and -2.51±0.232㎜ in the control group. The difference between groups was significant(p=0.001), favoring diclofenac sodium for maintaining pupil dilatation