Effects of Different Fatty Acids and Levels on the Lipogenesis Capacity and Lipolysis Rate of Broilers In Vitro

Lien, T.F.,Wu, C.P.,Chen, K.L.,Yang, K.H. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.9

This study investigated the lipogenesis capacity of hepatocytes and lipolysis rate of adipocytes of broilers as affected by different fatty acids (trial one) and different linoleic acid (C18:2) levels (trial two). Twenty 6-wk old broilers were used; their hepatocytes and adipocytes were isolated for the in vitro study. In trial one, four treatments were tested. The control group in which no fatty acid was added, and the test groups to which were added $300{\mu}M$ of C16:0, C18:1 and C18:2, respectively. For trial two, different levels (0, $300{\mu}M$ and 1 mM) of C18:2 combined to fatty acid-free bovine serum albumin (BSA) were added to the medium. According to results of trial one, added fatty acids significantly reduced the incorporation by hepatocytes of [U,$^{14}C$]glucose into total lipid (p<0.05); the lipogenesis capacity in C18:2 group was the lowest. Although a similar pattern was found with [l,$^{14}C$]acetate, the groups only slightly differed in terms of lipogenesis capacity (p=0.11). In addition, the C18:2 group had a significantly (p<0.05) greater lipolysis rate than the C16:0 and control groups. Results of trial two indicated that C18:2 significantly (p<0.05) reduced lipogenesis capacity both for [U,$^{14}C$]glucose and [l,$^{14}C$]acetate, and markedly stimulated the lipolysis rate (p<0.05), displaying a dose response. Results presented herein demonstrate that C18:2 can reduce lipogenesis capacity and stimulate the lipolysis rate in broilers.

C₂H <i>N</i> = 1 − 0 and N₂H⁺<i>J</i> = 1 − 0 observations of <i>Planck</i> Galactic cold clumps

Liu, X.-C.,Wu, Y.,Zhang, C.,Liu, T.,Yuan, J.,Qin, S.-L.,Ju, B.-G.,Li, L.-X. EDP Sciences 2019 Astronomy and astrophysics Vol.622 No.-

<P>A survey of C2H <I>N</I> = 1 − 0 and N2H<SUP>+</SUP><I>J</I> = 1 − 0 toward <I>Planck</I> Galactic cold clumps (PGCCs) was performed using the Purple Mountain Observatory’s 13.7 m telescope. C2H and N2H<SUP>+</SUP> were chosen to study the chemical evolutionary states of PGCCs. Among 121 observed molecular cores associated with PGCCs, 71 and 58 are detected with C2H <I>N</I> = 1 − 0 and N2H<SUP>+</SUP><I>J</I> = 1 − 0, respectively. The detected lines of most sources can be fitted with a single component with compatible <I>V</I>LSR and line widths, which confirms that these PGCC cores are very cold (with gas temperatures 9-21 K) and quiescent while still dominanted by turbulence. The ratio between the column densities of C2H and N2H<SUP>+</SUP> (<I>N</I>(C2H)/<I>N</I>(N2H<SUP>+</SUP>)) is found to be a good tracer for the evolutionary states of PGCC cores. Gas-grain chemical model can reproduce the decreasing trend of <I>N</I>(C2H)/<I>N</I>(N2H<SUP>+</SUP>) as a function of time. The cores with the lowest abundances of N2H<SUP>+</SUP> (<I>X</I>[N2H<SUP>+</SUP>] < 10<SUP>−10</SUP>) are the youngest, and have nearly constant abundances of C2H. In evolved cores with <I>X</I>[N2H<SUP>+</SUP>] ~10<SUP>−9</SUP>, abundances of C2H drop quickly as the exhaustion of carbon atoms. Although these PGCC cores are in different evolutionary states, they are all quite young (< 5 × 10<SUP>5</SUP> yr) with <I>N</I>(C2H) > <I>N</I>(N2H<SUP>+</SUP>). Mapping observations are carried out toward 20 PGCC cores. The PGCC cores in Cepheus have lower <I>N</I>(C2H)/<I>N</I>(N2H<SUP>+</SUP>) and larger line widths compared with those in Taurus. This implies that PGCC cores in Taurus are less chemically evolved than those in Cepheus.</P>

Distribution of stress state in the Nankai subduction zone, southwest Japan and a comparison with Japan Trench

Lin, W.,Byrne, T.B.,Kinoshita, M.,McNeill, L.C.,Chang, C.,Lewis, J.C.,Yamamoto, Y.,Saffer, D.M.,Casey Moore, J.,Wu, H.Y.,Tsuji, T.,Yamada, Y.,Conin, M.,Saito, S.,Ito, T.,Tobin, H.J.,Kimura, G.,Kanagaw Elsevier Scientific Publishing Co 2016 Tectonophysics Vol.692 No.2

<P>To better understand the distribution of three dimensional stress states in the Nankai subduction zone, southwest Japan, we review various stress-related investigations carried out in the first and second stage expeditions of the Nankai Trough Seismogenic Zone Experiment (NanTroSEIZE) by the Integrated Ocean Drilling Program (IODP) and compile the stress data. Overall, the maximum principal stress sigma(l) in the shallower levels (<similar to 1 km) is vertical from near the center of forearc basin to near the trench and; the maximum horizontal stress S-Hmax (interpreted to be the intermediate principal stress sigma(2)) is generally parallel to the plate convergence vector. The exception to this generalization occurs along the shelf edge of the Nankai margin where S-Hmax, is along strike rather than parallel to the plate convergence vector. Reorientation of the principal stresses at deeper levels (e.g., >similar to 1 km below seafloor or in underlying accretionary prism) with sigma(1) becoming horizontal is also suggested at all deeper drilling sites. We also make a comparison of the stress state in the hanging wall of the frontal plate-interface between Site C0066 in the Nankai and Site C0019 in the Japan Trench subduction zone drilled after the 2011 Mw 9.0 Tohoku-Oki earthquake. In the Japan Trench, a comparison between stress state before and after the 2011 mega-earthquake shows that the stress changed from compression before the earthquake to extension after the earthquake. As a result of the comparison between the Nankai Trough and Japan Trench, a similar current stress state with trench parallel extension was recognized at both C0006 and C0019 sites. Hypothetically, this may indicate that in Nankai Trough it is still in an early stage of the interseismic cycle of a great earthquake which occurs on the decollement and propagates to the toe (around site C0006). (C) 2015 Elsevier B.V. All rights reserved.</P>

Hexacosanol reduces plasma and hepatic cholesterol by activation of AMP-activated protein kinase and suppression of sterol regulatory element-binding protein-2 in HepG2 and C57BL/6J mice

Lee, J.H.,Jia, Y.,Thach, T.T.,Han, Y.,Kim, B.,Wu, C.,Kim, Y.,Seo, W.D.,Lee, S.J. Pergamon Press 2017 Nutrition research Vol.43 No.-

<P>Policosanols have hypocholesterolemic activity; however, the molecular mechanism of the policosanol effects is currently poorly characterized. We hypothesized that hexacosanol, a policosanol compound derived from barley sprout, may decrease cellular and plasma cholesterol levels; we thus investigated the hypocholesterolemic activity and mechanism of hexacosanol on both hepatocytes and high-fat-induced obese C57BL/6J mice. The reduction of total cholesterol, free cholesterol, and cholesteryl ester concentrations was confirmed in hexacosanol-stimulated hepatocytes (-38%, -33%, and -53%, respectively). Plasma, hepatic cholesterol concentrations, and hepatic steatosis were significantly reduced in high-fat-fed mice orally administered with hexacosanol (0.7 mg/kg body weight a day) for 8 weeks compared with those of vehicle-fed control mice (-15% and -40%, respectively). Hexacosanol in fact bound to the allosteric regulation site of AMP-activated protein kinase (AMPK)-beta subunit and thus activated AMPK that inhibited the activity of 3-hydroxy-3methyl-glutaryl-coenzyme A reductase by inhibitory phosphorylation. In addition, activation of AMPK by hexacosanol induced hepatic autophagy activity, which could further reduce hepatic lipid accumulation. Alternatively, hexacosanol suppressed the nuclear translocation and activation of sterol regulatory element-binding protein-2 (SREBP-2), a key transcription factor in cholesterol biosynthesis. These results collectively suggest that hexacosanol is a major hypocholesterolemic compound in barley sprouts with regulation of AMPK activation and SREBP-2 suppression. These suppress 3-hydroxy-3-methyl-glutarylcoenzyme A reductase at both mRNA expression and protein activity levels. In conclusion, hexacosanol activates AMPK and hepatic autophagy and inhibits SREBP2, resulting in hypocholesterolemic activities and improvement of hepatic steatosis. (C) 2017 Published by Elsevier Inc.</P>

Activation of Akt as a mechanism for tumor immune evasion.

Noh, Kyung Hee,Kang, Tae Heung,Kim, Jin Hee,Pai, Sara I,Lin, Ken Y,Hung, Chien-Fu,Wu, T-C,Kim, Tae Woo Academic Press 2009 Molecular therapy Vol.17 No.3

<P>Immune evasion is an important reason why the immune system cannot control tumor growth. To elucidate the mechanism for tumor immune evasion, we generated an immune-resistant human papillomavirus type 16 (HPV-16) E7-expressing tumor cell line by subjecting a susceptible tumor cell line to multiple rounds of in vivo immune selection with an E7-specific vaccine. Comparison of parental and immune-resistant tumors revealed that Akt is highly activated in the immune-resistant tumors. Retroviral transfer of a constitutively active form of Akt into the parental tumor significantly increased its resistance against E7-specific CD8(+) T-cell mediated apoptosis. The observed resistance against apoptosis was found to be associated with the upregulation of antiapoptotic molecules. We also observed that intratumoral injection of an Akt inhibitor enhanced the therapeutic efficacy of E7-specific vaccine or E7-specific CD8(+) T-cell adoptive transfer against the immune-resistant tumors. Thus, our data indicate that the activation of PI3K/Akt pathway represents a new mechanism of immune escape and has important implications for the development of a novel strategy in cancer immunotherapy against immune-resistant tumor cells.</P>

다이캐스팅한 SiC 입자강화 알루미늄 복합재료의 피로균열 전파특성

김송희,윤여범,이기호,오준도,주희위,이지환 대한금속재료학회(대한금속학회) 1998 대한금속·재료학회지 Vol.36 No.8

This study is to investigate the effect of load ratio on fatigue crack of die-cast SiC particulate aluminum composites. Fundamental fatigue crack propagation tests were performed with sub-sized C-T specimens at a load ratio of 0.1, 0.3 and 0.5 respectively. Crack closure measurements were conducted for explaning the effect of load ratio on fatigue crack growth. The experimental results showed that the fatigue crack growth rate increases with the load ratio, especially at near threshold. The composite reinforced with 20 vol.% SiC particles was better in fatigue crack growth resistance than that with 10 vol.% SiC particles. Through the measurement of Kop and ΔK at various R ratios the concept of effective stress intensity factor range ratio. U was reviewed to evaluate the stress ratio effect on fatigue crack growth. Relationships between U and variables such as ΔK and R were obtained empirically so that we could predict ΔK_(eff) that is of critical importance for the prediction of fatigue crack propagation rate.

Fiber hypertrophy and increased oxidative capacity can occur simultaneously in pig glycolytic skeletal muscle

Scheffler, T. L.,Scheffler, J. M.,Park, S.,Kasten, S. C.,Wu, Y.,McMillan, R. P.,Hulver, M. W.,Frisard, M. I.,Gerrard, D. E. American Physiological Society 2014 American journal of physiology. Cell physiology Vol.306 No.4

<P>An inverse relationship between skeletal muscle fiber cross-sectional area (CSA) and oxidative capacity suggests that muscle fibers hypertrophy at the expense of oxidative capacity. Therefore, our objective was to utilize pigs possessing mutations associated with increased oxidative capacity [AMP-activated protein kinase (AMPKγ<SUB>3</SUB><SUP>R200Q</SUP>)] or fiber hypertrophy [ryanodine receptor 1 (RyR1<SUP>R615C</SUP>)] to determine if these events occur in parallel. Longissimus muscle was collected from wild-type (control), AMPKγ<SUB>3</SUB><SUP>R200Q</SUP>, RyR1<SUP>R615C</SUP>, and AMPKγ<SUB>3</SUB><SUP>R200Q</SUP>-RyR1<SUP>R615C</SUP> pigs. Regardless of AMPK genotype, RyR<SUP>R615C</SUP> increased fiber CSA by 35%. In contrast, AMPKγ<SUB>3</SUB><SUP>R200Q</SUP> pig muscle exhibited greater citrate synthase and β-hydroxyacyl CoA dehydrogenase activity. Isolated mitochondria from AMPKγ<SUB>3</SUB><SUP>R200Q</SUP> muscle had greater maximal, ADP-stimulated oxygen consumption rate. Additionally, AMPKγ<SUB>3</SUB><SUP>R200Q</SUP> muscle contained more (∼50%) of the mitochondrial proteins succinate dehydrogenase and cytochrome <I>c</I> oxidase and more mitochondrial DNA. Surprisingly, RyR1<SUP>R615C</SUP> increased mitochondrial proteins and DNA, but this was not associated with improved oxidative capacity, suggesting that altered energy metabolism in RyR1<SUP>R615C</SUP> muscle influences mitochondrial proliferation and protein turnover. Thus pigs that possess both AMPKγ3<SUP>R200Q</SUP> and RyR<SUP>R615C</SUP> exhibit increased muscle fiber CSA as well as greater oxidative capacity. Together, our findings support the notion that hypertrophy and enhanced oxidative capacity can occur simultaneously in skeletal muscle and suggest that the signaling mechanisms controlling these events are independently regulated.</P>

Low Resistance and Transparent Ag/AZO Ohmic Contact to p-GaN

T. Han,T. Wang,X. W. Gan,H. Wu,Y. Shi,C. Liu 한국물리학회 2014 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.65 No.1

Silver (Ag)/ aluminum-doped zinc oxide (AZO) films were deposited on p-GaN by using electronbeamevaporation. After the annealing process, current -voltage (I-V) measurements were carriedout to determine the characteristic of the contacts. The Ag/AZO films annealed at 600 C werefound to present an ohmic contact behavior. The specific contact resistance was calculated tobe 9.76 × 10−4cm2 and the transmittance was over 80% for visibly light. The atomic forcemicroscope was used to measure the aggregation of Ag grains which may have been the main factorin the formation of the Ag/AZO ohmic contact to p-GaN.

A Meshfree procedure for the microscopic analysis of particle-reinforced rubber compounds

Wu, C.T.,Koishi, M. Techno-Press 2009 Interaction and multiscale mechanics Vol.2 No.2

This paper presents a meshfree procedure using a convex generalized meshfree (GMF) approximation for the large deformation analysis of particle-reinforced rubber compounds on microscopic level. The convex GMF approximation possesses the weak-Kronecker-delta property that guarantees the continuity of displacement across the material interface in the rubber compounds. The convex approximation also ensures the positive mass in the discrete system and is less sensitive to the meshfree nodal support size and integration order effects. In this study, the convex approximation is generated in the GMF method by choosing the positive and monotonic increasing basis function. In order to impose the periodic boundary condition in the unit cell method for the microscopic analysis, a singular kernel is introduced on the periodic boundary nodes in the construction of GMF approximation. The periodic boundary condition is solved by the transformation method in both explicit and implicit analyses. To simulate the interface de-bonding phenomena in the rubber compound, the cohesive interface element method is employed in corporation with meshfree method in this study. Several numerical examples are presented to demonstrate the effectiveness of the proposed numerical procedure in the large deformation analysis.

Parametric Simulations of Skidmark Formation on a Slab in a Reheating Furnace : Effects of Skid-Button Height and Skid Pipe Gap

C. W. Wang,B. Y. Yang,C. Y. Wu,C. J. Ho,T. Y. Ho 대한기계학회 1993 International Symposium on Transport Phenomena in Vol.2 No.1