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Characterization of Novel Plasmid p1B146 from Corynebacterium tuberculostearicum
( Wieteska Lukasz ),( Eligia M. Szewczyk ),( Janusz Szemraj ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.8
Corynebacterium tuberculostearicum B146, a strain derived from healthy human skin, contains a medium copy plasmid, p1B146. This plasmid was cloned and its complete nucleotide sequence determined. As a result, p1B146 was found to be 4,2 kb in size with a 53% G+C content, plus six open reading frames (ORFs) were distinguished. According to a computer-assisted alignment, two of the ORFs exhibited significant similarities to already-known common plasmid proteins, the first being the RepA gene, responsible for plasmid replication via a rolling-circle mechanism, and the second being an FtsK-like protein, the function of which remains unclear. The presence and quantity of RNA fragments in the putative ORFs were also evaluated.
Bożena Pawłowska,Marek Żupnik,Bożena Szewczyk-Taranek,Monika Cioć 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.1
Gerbera jamesonii cv. Dura is a well-known and economically important ornamental crop that is produced using micropropagation. This study aimed to evaluate the effect of various light wavelengths in six different light emitting diode (LED)treatments on biometric attributes and photosynthetic pigment content in gerbera plants grown in vitro. The LED treatmentswere B (100% blue), RB1 (50% red and 50% blue), RB2 (70% red and 30% blue), RBW (40% red, 40% blue, and 20% white),RBfR (49% red, 49% blue, and 2% far red), and R (100% red). Light quality was seen to significantly affect plant growthand development. The highest shoot multiplication rate was observed in plants grown under both RB1 and RB2. Plantletsgrown under R displayed the greatest shoot elongation, and their petioles were three times longer than those grown underB. Both B and R resulted in reduced leaf blade area. Rooting of shoots was observed in plants grown under all light treatments;however, R stimulated adventitious root formation. The average number of roots produced by plants under all lighttreatments was calculated and found to match that produced under RB2. The RBfR treatment caused a reduction in leaf dryweight compared to that produced under B, which represented the highest leaf dry weight. Control fluorescent lighting, suppliedby Philips TL-D 36W/54 lamps, had a positive effect on root dry weight. Photosynthetic pigment content was higherin the leaves of rooted plants compared to that in plants at the multiplication stage. RB2 resulted in higher concentrations ofchlorophyll a and b and carotenoids, whereas lower accumulation of photosynthetic pigments was observed under R. Theseresults demonstrate that light wavelength manipulation through LEDs can be strategically used for the rapid and large-scalepropagation of gerbera. The outcomes of this study offer potential to improve micropropagation efficiency and reduce thecosts of in vitro plant production.
Towards defi ning a simplifi ed procedure for COTS system-on-chip TID testing
Stefano Di Mascio,Alessandra Menicucci,Gianluca Furano,Tomasz Szewczyk,Luigi Campajola,Francesco Di Capua,Andrea Lucaroni,Marco Ottavi 한국원자력학회 2018 Nuclear Engineering and Technology Vol.50 No.8
The use of System-on-Chip (SoC) solutions in the design of on-board data handling systems is animportant step towards further miniaturization in space. However, the Total Ionizing Dose (TID) andSingle Event Effects (SEE) characterization of these complex devices present new challenges that areeither not fully addressed by current testing guidelines or may result in expensive, cumbersome testconfigurations. In this paper we report the test setups, procedures and results for TID testing of a SoCmicrocontroller both using standard 60Co and low-energy protons beams. This paper specifically pointsout the differences in the test methodology and in the challenges between TID testing with proton beamand with the conventional gamma ray irradiation. New test setup and procedures are proposed whichare capable of emulating typical mission conditions (clock, bias, software, reprogramming, etc.) whilekeeping the test setup as simple as possible at the same time.
The Broad Variability in Dental Age Observed among Childhood Survivors Is Cancer Specific
Patrycja Proc,Joanna Szczepanska,Malgorzata Zubowska,Beata Zalewska-Szewczyk,Wojciech Mlynarski 대한암학회 2021 Cancer Research and Treatment Vol.53 No.1
Purpose The study aimed to assess the differences in dental maturation between childhood cancer survivors and healthy children. Materials and Methods Fifty-nine cancer patients including 16 (27.1%) girls and 43 (72.8%) boys, aged between 4 and 16 years, underwent dental and radiographic examinations. The mean duration of anticancer therapy was 16.8 months (range, 1 to 47 months), and 4.6 years (range, 8 to 123 months) had passed since the termination of disease. The control group consisted of 177 panoramic radiographs of age- and sex-matched healthy individuals. Dental age (DA) was estimated with Demirjian’s scale and delta age, i.e., DA–chronological age (CA), was used to compare groups. Results The DA of cancer survivors was accelerated by almost 1 year compared to their CA (9.9±3.1 vs. 8.9±2.8, p=0.040). The greatest difference was observed among patients with brain tumor: delta (DA–CA) was 2.2±1.1 years. Among all cancer patients, only children with familial adenomatous polyposis (FAP)-associated hepatoblastoma (HP) demonstrated delayed DA, with regard to both other cancer survivors (p=0.011) and healthy patients (p=0.037). All four patients with HP suffered from FAP, and three of them had documented adenomatous polyposis coli (APC) genes mutation. The DA of cancer patients having teeth with short roots was significantly greater than that of the cancer survivors without this anomaly (12.8±3.2 vs. 9.0±2.4, p < 0.001). Conclusion DA in children may be altered by cancer disease.
Hwang, K.‐,H.,Han, C.,Udalski, A.,Sumi, T.,Gould, A.,Jaroszyń,ski, M.,Kubiak, M.,Szymań,ski, M. K.,Pietrzyń,ski, G.,Soszyń,ski, I.,Szewczyk, O.,Ulaczyk, K.,Wyrzykowski, Blackwell Publishing Ltd 2011 MONTHLY NOTICES- ROYAL ASTRONOMICAL SOCIETY Vol.413 No.2
<P><B>ABSTRACT</B></P><P>We report the result of the analysis of the light curve of a caustic‐crossing binary‐lens microlensing event OGLE‐2009‐BLG‐023/MOA‐2009‐BLG‐028. Even though the event was observed solely by survey experiments, we could uniquely determine the mass of the lens and distance to it by simultaneously measuring the Einstein radius and lens parallax. From this, we find that the lens system is composed of M‐type dwarfs with masses (0.50 ± 0.07) and (0.15 ± 0.02) M<SUB>⊙</SUB> located in the Galactic disc with a distance of ∼1.8 kpc toward the Galactic bulge direction. The event demonstrates that physical lens parameters of binary‐lens events can be routinely determined from future high‐cadence lensing surveys and thus microlensing can provide a new way to study Galactic binaries.</P>
OGLE-2005-BLG-071Lb, THE MOST MASSIVE M DWARF PLANETARY COMPANION?
Dong, Subo,Gould, Andrew,Udalski, Andrzej,Anderson, Jay,Christie, G. W.,Gaudi, B. S.,Jaroszyń,ski, M.,Kubiak, M.,Szymań,ski, M. K.,Pietrzyń,ski, G.,Soszyń,ski, I.,Szewczyk, O.,Ulac IOP Publishing 2009 The Astrophysical journal Vol.695 No.2
OGLE-2005-BLG-153: MICROLENSING DISCOVERY AND CHARACTERIZATION OF A VERY LOW MASS BINARY
Hwang, K.-H.,Udalski, A.,Ryu, Y.-H.,Bond, I. A.,Beaulieu, J.-P.,Dominik, M.,Horne, K.,Gould, A.,Gaudi, B. S.,Kubiak, M.,Szymań,ski, M. K.,Pietrzyń,ski, G.,Soszyń,ski, I.,Szewczyk, O.,Ul IOP Publishing 2010 The Astrophysical journal Vol.723 No.1
Han, C.,Hwang, K.-H.,Kim, D.,Udalski, A.,Abe, F.,Monard, L. A. B.,McCormick, J.,Szymań,ski, M. K.,Kubiak, M.,Pietrzyń,ski, G.,Soszyń,ski, I.,Szewczyk, O.,Wyrzykowski, Ł.,Ulaczyk, K.,Bon IOP Publishing 2009 The Astrophysical journal Vol.705 No.2
<P>To improve the planet detection efficiency, current planetary microlensing experiments are focused on high-magnification events searching for planetary signals near the peak of lensing light curves. However, it is known that signals from those induced by binary companions. In this paper, we analyze the light curves of microlensing events OGLE-2007-BLG-137/MOA-2007-BLG-091, OGLE-2007-BLG-355/MOA-2007-BLG-278, and MOA-2007- BLG-199/OGLE-2007-BLG-419, for all of which exhibit short-term perturbations near the peaks of the light curves. From detailed modeling of the light curves, we find that the perturbations of the events are caused by binary companions rather than planets. From a close examination of the light curves combined with the underlying physical geometry of the lens system obtained from modeling, we find that the short timescale caustic-crossing feature occurring at a low or a moderate base magnification with an additional secondary perturbation is a typical feature of binary-lens events and thus can be used for the discrimination between the binary and planetary interpretations.</P>