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        Impact of LED light sources on morphogenesis and levels of photosynthetic pigments in Gerbera jamesonii grown in vitro

        Bożena Pawłowska,Marek Żupnik,Bożena Szewczyk-Taranek,Monika Cioć 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.1

        Gerbera jamesonii cv. Dura is a well-known and economically important ornamental crop that is produced using micropropagation. This study aimed to evaluate the effect of various light wavelengths in six different light emitting diode (LED)treatments on biometric attributes and photosynthetic pigment content in gerbera plants grown in vitro. The LED treatmentswere B (100% blue), RB1 (50% red and 50% blue), RB2 (70% red and 30% blue), RBW (40% red, 40% blue, and 20% white),RBfR (49% red, 49% blue, and 2% far red), and R (100% red). Light quality was seen to significantly affect plant growthand development. The highest shoot multiplication rate was observed in plants grown under both RB1 and RB2. Plantletsgrown under R displayed the greatest shoot elongation, and their petioles were three times longer than those grown underB. Both B and R resulted in reduced leaf blade area. Rooting of shoots was observed in plants grown under all light treatments;however, R stimulated adventitious root formation. The average number of roots produced by plants under all lighttreatments was calculated and found to match that produced under RB2. The RBfR treatment caused a reduction in leaf dryweight compared to that produced under B, which represented the highest leaf dry weight. Control fluorescent lighting, suppliedby Philips TL-D 36W/54 lamps, had a positive effect on root dry weight. Photosynthetic pigment content was higherin the leaves of rooted plants compared to that in plants at the multiplication stage. RB2 resulted in higher concentrations ofchlorophyll a and b and carotenoids, whereas lower accumulation of photosynthetic pigments was observed under R. Theseresults demonstrate that light wavelength manipulation through LEDs can be strategically used for the rapid and large-scalepropagation of gerbera. The outcomes of this study offer potential to improve micropropagation efficiency and reduce thecosts of in vitro plant production.

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