인플루엔자 바이러스의 Neuraminidase 抗原分析

白承福 건국대학교 1976 論文集 Vol.3 No.1

1.The influenza virus strains isolated in Korea in 1971 and 1972 were experimented for their antigenic analysis by Neuraminidase-Inhibition test. 2.A/NWS(H0N2), a variant strain of A/Pa/8/34(H0N1) was used as a N2 reference virus strain and A/0318(H0N2), a recombinant strain of AJNWS(H0N2) and A/Aichi/2/68 (H3N2) was used as a Na reference virus strain. Strain specific antisera were prepared in adult chicken in which H-I titer of 1 : 2048 or more were used as for reference antisera. 3.The obtained results of the tests are summarized as follows : all the methods used in the tests were followed by standard methods of Center far Disease Control in USA. a) Neuraminidase assay tests ; The virus dilution numbers corresponding to O.D. 0.850 of each strains were 1 : 8.5 in NWS(H0N1),1 :10 in 0318(H0N2), 1 :14 in A/Korea/71 and 1 : 7.4 in A/Korea/72. b) Neuraminidase-Inhibition tests ; The titers of 70% neuraminidase-inhibition activity of each antiserum against corresponding antigens were indicated in the following table. ◁표 삽입▷ (원문을 참조하세요) 4.As shown in the above table, it was identified that A/Korea/71 and A/Korea/72, isolated strains were belonged to N2 subtype of Influenza A.

칼슘수용액으로 처리한 상아질과 합착용 글래스아이오노머의 전단결합강도에 관한 연구

백영걸,이성복,박남수 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.1

The objective of this paper was to evaluate the shear bond strength of luting glass ionomer cement with differ calcium based solution treatment on dentin surface. 120 extracted human teeth were classified into 12 group on presence of smear layer on dentin surface and type of treatment solution. Smear layer remove on dentin surfas was done using 6% citric acid for 60 seconds. Five different dentin surface treatment solutions(calcium acetaa calcium carbonate, calcium chloride, calcium hydroxide, and calcium phosphate) were evaluated in this study! After surface modification, metal ring(inner diameter: 3mm, depth:1mm) was placed to expose the same dentin surface area and inner space was filled with luting glass ionomer cement according to the recommended procedure for standard clinical procedure. The shear bond strength of glass ionomer cement was determined after 24 hours. SEM was used for the evaluation of the surface morphologic changes and EDAX analysis was done. for determination of the change of the calcium contents of treated dentin. Following conclusions can be drawn: 1. In the group of the dentin surface with smear layer, the calcium carbonate solution was the most effective for the increase of the calcium content and the shear bond strength of glass ionomer cement to dentin surfaces. 2. In the group of the calcium carbonate treated dentin with smear layer, the shear bond strength was increased two compared to the control group and cohesive failure mode was observed. 3. The shear bond strength of cement was increased significantly by the removal of smear layer using 6% citric acid. However, additional calcium solution treatments were not effective for further bond strength increase 4. The shear bond strength of cement was significantly improved by both of the removal of smear layer and the calcium solution treatment, and the former was more effective for bond strength improvement 5. The smear layer removed/calcium solution treated groups showed dentinal tubule obstruction and crystiattachment in SEM evaluation. However, the shear bond strengths of these groups were not increased compared to the smear layer removed/no dentin treatment group.

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

사이토카인과 Lipopolysaccharide 자극에 의한 RAW 264.7 세포주의 nitric oxide 생성

김영덕,전창덕,이병순,이복수,박석돈,백상기,정헌택 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

Macrophages have been implicated as a major class of effector in the host response to neoplasia. Cells of the monocyte-macrophage lineage are known to exhibit tumoricidal activity following stim-ulation by γ-interferon, tumor necrosis factor, BCG and bacterial products such as lipopoly-saccharide (LPS). While the mechanism involved remain obscure, the generation of reactive nitrogen intermediate (RNI) by activated macrophages is considered a maior participant in mediating the tumorstatic effect. But much of what is known about the induction and release of RNI has been elucidated by using freshly isolated cells from blood and other tissues of experimental animals. In this study, we used a murine macrophage cell line, RAW 264.7, and found that these cells showed above 99% positive of pan macrophage marker by immunohistochemical staining. These cells could produce nitric oxide (NO), when incubated with γ-IFN or poly I:C. Incubation of RAW 264.7 cells with γ-IFN for 48 hous in the presence of LPS agumented NO release in a dose dependent manner. Whereas, treatment of anti-TNE-α antibody or antisense TNF-α oligodeoxynucleotide inhibited the release of NO_2 by γ-IFN plus LPS activated macrophages. The production of NO was also inhibited reversibly or irreversibly by N^GMMA,NAA,arginase or DPI. Thease data suggest that RAW 264.7 cell line may be useful for the in vitro evalulation of biological response modifiers as well as the study of signal pathway of NO release by macrophages.

Gravitational Wave Astrophysics with the Superconducting Low-frequency Gravitational-wave Telescope

Sang-Hyeon Ahn,Yeong-Bok Bae,Gungwon Kang,Chunglee Kim,Whansun Kim,John J. Oh,Sang Hoon Oh,Chan Park,Edwin J. Son,Hyung Mok Lee,Hyungwon Lee,Hyunkyu Lee,Chang-Hwan Lee,Ho Jung Paik 한국천문학회 2017 天文學會報 Vol.42 No.2

日本腦炎백신 製造에 關한 硏究 : Ⅲ.人體接種試驗 Ⅲ. Field trials

白承福,申鎭澈,鄭義範 國立保健硏究院 1970 국립보건연구원보 Vol.7 No.-

다중화 방식을 활용한 UHDTV 전송을 위한 시스템 설계 및 성능 평가

최성복(Sung-Bok Choi),송형규(Hyoung-Kyu Song),김용환(Yong-Hwan),백종호(Jong-Ho Paik),권기원(Ki-Won Kwon) 한국통신학회 2014 한국통신학회 학술대회논문집 Vol.2014 No.6

Production of Anti-Hepatitis B Surface Antigen Monoclonal Antibody in Serum-Free Medium

Chun, Bok Hwan,Jo, Eui Cheol,Kim, Dong Il,Paik, Sung Bok 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.2

간염 바이러스 표면 항원에 대한 단일클론 항체를 생산하는 하이브리도마 세포 2c3.1의 대량배양을 위하여 무혈청 배지를 조성하였다. 2c3.1 세포를 RPM1 1640 배지와 Ham's F12 배지의 여러 부피비에 배양함으로써 세포성장이 가장 좋은 비의 배지를 기본 영양배지로 선택하였다. 세포 성장을 증가시키기 위하여 무혈청 배지 첨가물들을 여러 농도로 변화시켜 기본영양배지에 첨가함으로써 각 첨가물들의 농도를 결정하였다. 무혈정 배지 첨가물은 0.45% 인혈청 알부민, 10㎍/㎖ insulin, 10㎍/㎖ transferrin 등을 사용하고, 0.17㎍/㎖ fat emulsion(Intralipos^R:0.125㎍/㎖ soybean oil, 0.015㎍/㎖ ovolecithin, 0.03㎍/㎖ glycerin), 0.01㎍/㎖ 비타민 E와 0.02㎍/㎖ 비타민 E 아세테이트, 그리고 10uM monoethanolamine 등의 적정 농도를 2c3.1 세포의 무혈청 배양에 사용하였다. 이 무혈정 배지에서 대수 증가적으로 증식하는 하이브리도마 세포는 10% 우태아혈청을 사용한 배지에서 보다 비증식속도와 최대 세포농도에서 다소 낮으나, 단일클론항체는 혈청 사용 배지의 43㎍/㎖보다 다소 높은 50㎍/㎖가 생성됨으로써 이 하이브리도마로부터 단일클론항체를 생산하기 위하여 조성된 무혈정 배지가 10% 우태아혈청 배지를 대신할 수 있음을 보였다. For the large-scale cultivation of murine hybridoma 2c3.1 cells secreting anti-Hepatitis B surface antigen(anti-HBsAg)monoclonal antibody(MAb), we have constructed a serum-free medium. The serum-free medium was supplemented with human serum albumain, insulin, transferrin, fat emulsion(Intralipos^R:soybean oil, ovolecithin, glycerin), vitamin E, vitamin E acetate, and monoethanolamine in a mixture of RPMI 1640 and Ham's F12 medium. The cells in serum-free medium propagated logarithmically without lag period, and the maximum concentration of anti-HBsAg monoclonal antibodies secreted by 2c3.1 cells was higher than that in 10% fetal bovine serum(FBS) medium. The serum-free medium was enough to substitute 10% FBS medium in respect of MAb production.

원발성 종격동 내배엽동종 1 예

김성규,조남훈,한승희,김세규,이원영,황성철,장준,백승,이유복 대한내과학회 1988 대한내과학회지 Vol.35 No.4

The endodermal sinus, or yolk sac tumor is a highly malignant and rare form of embryonal cell carcinoma which originates from the extraembryonic structure, as is choriocarcinoma, and occurs almost exclusively in males during the second and third decade of life, although it has been reported in children. In addition to the ovary and testis, endodermal sinus tumors have been described in extragonadal sites such as the sacrococcygeal region, the region of the pineal gland, the retroperitoneum and anterior mediastinum. We experienced a case of a primary mediastinal endodermal sinus tumor which occurred in a 29-year old male patient and was diagnosed by mediastinotomy. The patient has been treated with two courses of combination chemotherapy (vincristine, actinomycin-D and cyclophosphamide) and his condition has been followed up for 5 months.

Screening of a Specific Point Mutation in Tumor Suppressor p53 Gene of Korean Hepatocellular Carcinoma Tissues

Lee, Bok-Soo,Jun, Chang-Duk,Chun, Young-Whan,Paik, Sang-Gi,Cho, Baik-Whan,Choi, Chan,Chae, Kwon-Mook,Chung, Hun-Taeg 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-

The point mutation at a specific site (the third base of codon 249 of exon 7) in the p53 gene was not found in the 8 hepatocellular carcinoma samples from Korean patients. This result is quite different from the report on Chinese and South African patients that showed the point mutations at the same site with the frequency of 50% in hepatocellular carcinoma sample. Even though this particular point mutation was not found in Korean samples, there might be mutations at other sites of p53 gene, because expression of mutated p53 gene was detected in the nuclei of hepatocellular carcinoma samples by using monoclonal antibodies which are specific for mutant-type p53 proteins. Also the change of DNA content was found in the hepatocellular carcinoma samples.