Heme oxygenase-1 (HO-1)/carbon monoxide (CO) axis suppresses RANKL-induced osteoclastic differentiation by inhibiting redox-sensitive NF-κB activation

( Sun-uk Bak ),( Suji Kim ),( Hae-jun Hwang ),( Jung-a Yun ),( Wan-sung Kim ),( Moo-ho Won ),( Ji-yoon Kim ),( Kwon-soo Ha ),( Young-guen Kwon ),( Young-myeong Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2017 BMB Reports Vol.50 No.2

Heme oxygenase (HO-1) catalyzes heme to carbon monoxide (CO), biliverdin/bilirubin, and iron and is known to prevent the pathogenesis of several human diseases. We assessed the beneficial effect of heme degradation products on osteoclastogenesis induced by receptor activator of NF-κB ligand (RANKL). Treatment of RAW264.7 cells with CORM-2 (a CO donor) and bilirubin, but not with iron, decreased RANKLinduced osteoclastogenesis, with CORM-2 having a more potent anti-osteogenic effect. CORM-2 also inhibited RANKLinduced osteoclastogenesis and osteoclastic resorption activity in marrow-derived macrophages. Treatment with hemin, a HO-1 inducer, strongly inhibited RANKL-induced osteoclastogenesis in wild-type macrophages, but was ineffective in HO-1^+/-cells. CORM-2 reduced RANKL-induced NFATc1 expression by inhibiting IKK-dependent NF-κB activation and reactive oxygen species production. These results suggest that CO potently inhibits RANKL-induced osteoclastogenesis by inhibiting redox-sensitive NF-κB-mediated NFATc1 expression. Our findings indicate that HO-1/CO can act as an antiresorption agent and reduce bone loss by blocking osteoclast differentiation. [BMB Reports 2017; 50(2): 103-108]

소규모 하수처리를 위한 파일럿 규모 이중슬러지 KNR^® (Kwon's nutrient removal) 시스템의 영얌염류 제거성능 평가

안진영,권중천,김윤학,정유훈,김두언,유선호,김병우,An, Jin-Young,Kwon, Joong-Chun,Kim, Yun-Hak,Jeng, Yoo-Hoon,Kim, Doo-Eon,Ryu, Sun-Ho,Kim, Byung-Woo 한국청정기술학회 2006 청정기술 Vol.12 No.2

본 연구에서 소규모 하수고도처리를 위한 이중슬러지(Dual sludge) $KNR^{(R)}$ (Kwon's nutrient removal) 시스템이 개발되었다. $KNR^{(R)}$ 시스템은 부유성장식 탈질미생물과 부착성장식 질산화미생물을 분리시킨 이중슬러지 공정으로 최초침전조, 혐기조, 무산소조, 농축조의 복합기능을 수행하는 UMBR (Upflow multi-layer bioreactor)과 펠렛형 담체가 충진된 호기성 담체조로 구성되어 있다. 소규모 하수처리시 본 개발공정의 안정성과 처리성능을 평가하기 위해 처리용량 $50m^3/d$ 규모의 파일럿 플랜트를 고도처리 공정으로 개선공사 중인 처리용량 $50m^3/d$ 규모의 실제 소규모 마을하수처리장에 적용하였다. UMBR과 담체조의 체류시간은 각각 4.7 h와 7.2h이었으며, 반응조 수온은 $18.1{\sim}28.1^{\circ}C$이었다. 유입 하수량과 유입수의 BOD/N의 변동폭이 컸음에도 불구하고 파일럿 플랜트는 안정된 처리성능을 보였다. 전체 실험기간 중 처리수의 $COD_{cr}$, $COD_{Mn}$, $BOD_5$, TN, TP의 평균 농도는 11.0 mg/L, 8.8 mg/L, 4.2 mg/L, 3.5 mg/L, 9.8 mg/L, 0.87/0.17 mg/L (poly aluminium chloride(PAC) 투입/미투입)이었으며, 제거율을 각각 95.3%, 87.6%, 96.3%, 96.5%, 68.2%, 55.4/90.3% 이었다. 잉여슬러지 발생량은 $A_2O$와 Bardenpho 등과 같은 단일슬러지를 이용하는 고도처리공정과 비교시 약 1.9~3.8배 낮은 $0.026kg-DS/m^3$ and 0.220 kg-DS/kg-BOD로 나타났다. A simple dual sludge process, called as $KNR^{(R)}$ (Kwon's Nutrient Removal) system, was developed for small sewage treatment. It is a hybrid system that consists of an UMBR (Upflow multi-layer bioreactor) as anaerobic and anoxic reactor with suspended denitrifier and a post aerobic biofilm reactor, filled with pellet-like media, with attached nitrifier. To evaluate the stability and performance of this system for small sewage treatment, the pilot-scale $KNR^{(R)}$ plant with a treatment capacity of $50m^3/d$ was practically applied to the actual sewage treatment plant, which was under retrofit construction during pilot plant operation, with a capacity of $50m^3/d$ in a small rural community. The HRTs of a UMBR and a post aerobic biofilm reactor were about 4.7 h and 7.2 h, respectively. The temperature in the reactor varied from $18.1^{\circ}C$ to $28.1^{\circ}C$. The pilot plant showed stable performance even though the pilot plant had been the severe fluctuation of influent flow rate and BOD/N ratio. During a whole period of this study, average concentrations of $COD_{cr}$, $COD_{Mn}$, $BOD_5$, TN, and TP in the final effluent obtained from this system were 11.0 mg/L, 8.8 mg/L, 4.2 mg/L, 3.5 mg/L, 9.8 mg/L, and 0.87/0.17 mg/L (with/without poly aluminium chloride(PAC)), which corresponded to a removal efficiency of 95.3%, 87.6%, 96.3%, 96.5%, 68.2%, and 55.4/90.3%, respectively. Excess sludge production rates were $0.026kg-DS/m^3$-sewage and 0.220 kg-DS/kg-BOD lower 1.9 to 3.8 times than those in activated sludge based system such as $A_2O$ and Bardenpho.

국내 살모넬라 분리주에서 spvR 유전자 분포와 단일 뉴클레오티드 다형 연구

권혁준,박경윤,박주연,박용호,김선중,유한상 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

목적 : 지금까지 병원성 살모넬라를 확인하기 위해 분자량이 큰 플라스미드의 존재 유무를 확인하여 왔으나 살모넬라 혈청형 및 검출 방법에 따라 양성율에 큰 차이를 보이고 있고, 일부 병원성 살모넬라 분리주에서 병원성 플라스미드가 관찰되지 않아 spv 유전자들의 유무에 대한 아른 확인방법이 필요하다. 본 실험은 국내 동물 유래 주요 살모넬라 혈청형에서 spvR 유전자의 분포를 알아보고, 병원성 살모넬라 분자진단을 위한 분자표지로써의 가치를 평가하기 위해 실행하였다. 재료 및 방법 : 국내 가축 유래 S. lyphimurium (ST, 26주), S. enteritidis (SE, 10주), S. pullorum (SP, 40주), S. gallinarum (SG, 53주)에 대해 GenBank에 등록된 ST, SE, SP의 spvR 유전자를 비교하여 ST와 SE 간에 차이를 보이는 single nucleotide polymorphism (SNP, 625번 뉴클레오티드)을 포함하도록 시발자를 합성하여 집락-PCR을 수행하였다. 증폭 산물(194bp)은 자동 염기서열장치를 이용하여 염기서열을 결정하였고, 제한효소인 MseI을 사용하여 PCR-RFLP를 실시하였다. 결과 : 집락-중합효소연쇄반응 결과 SP, SG, SE의 모든 (100%) 분리주에서 특이 증폭산물이 검출되었으나 ST의 경우 19주(73%)에서만 증폭되었다. 특히 병원성 관련 플라스미드가 관찰되지 않았던 SP 4주에서 특이적인 증폭산물이 검출되었다. 염기서열 분석결과 SE, SG, SP는 625번 뉴클레오티드에 아데닌을, ST는 구아닌을 가지고 있어 PCR-RFLP를 이용하여 쉽게 구분할 수 있었다. 결 론 : spvR에 대한 집락-중합효소연쇄 반응법은 병원성 플라스미드 검출법보다 SE, SP, SG 병원성 주를 신속하게 검출하는데 유용하고, 625번 뉴클레오티드의 SNP는 ST와 SE, SG, SP를 구분하는 분자표지로 사용될 수 있을 것으로 기대되며, ST에 비해 SE는 진화적으로 SG와 SP에 가까운 것으로 생각되었다. Background : The Salmonella virulence plasmid (spv) genes (spvR, A, B, C and D) on the large virulence plasmids of pathogenic Salmonella serotypes can replace the virulence of the whole plasmid. Recently, virulence plasmid-negative pathogenic Salmonella isolates were isolated. However, positive rates of spv genes among Korean Salmonella serotypes have been obscure. spv genes are conserved in compared to other virulence genes but there are single nucleotide polymorphisms (SNPs) conserved in only certain serotype. Such SNPs are useful for differentiation and understanding evolution of certain serotypes. Materials and Methods : Salmonella serotypes isolated from live stocks [Salmonella typhimurium (ST, 26), S. enteritidis (SE, 10), S. gallinarum (SG, 40) and S. pullorum (SP, 53)] were used for colony-PCR. A primer set covering single nucleotide polymorphism (SNP) at 625th nucleotide of spvR was designed. The nucleotide sequences of amplicons were determined by cyclic sequencing method and RFLP was performed by using MseI. Results : All isolates of SE, SG and SP, including four plasmid-negative isolates, showed specific amplicons but not all of ST (19/26, 73%) were positive to spvR. Based on the nucleotide sequence of 625th nucleotide and PCR-RFLP, SE, SG and SP [A(625)] and ST [G(625)] could be differentiated. Conclusion : spvR can be used as a molecular marker to detect virulent SE, SG, SP and the SNP may be useful for differentiation of SE, SG, SP and ST. According to the SNP study SE may be evolutionarily closer to SG and SP than ST.

박판스프링 교정시스템 구축에 관한 연구

권혁홍,김선호 대진대학교 1997 大眞論叢 Vol.5 No.-

This paper describes that study which construct a theoretic and experimental algorithm in order to make the automatic correction system of detent spring, and when load for correction pressed at spring, it can be found elastic and plastic deformation quantities by Finite Element Analysis. As a result, it has been found that the simulation datas are in good agreement with experimetal results.

만성부비동염 환자에서 부비동 단순 X-선 사진과 전산화 단층(CT) 사진 소견의 비교

선동일,윤선호,신민호,서병도,양일권 釜算慶南耳鼻咽喉科學會 1993 임상이비인후과 Vol.4 No.1

銅板핀이 附着된 水平圓筒에서의 自然對流 熱傳達

權純錫,金善政,洪南湖 동아대학교 공과대학 부설 한국자원개발연구소 1987 硏究報告 Vol.11 No.1

This study on the natural convection has been studied experimentally for the heat transfer characteristics of the horizontal cylinder with one copper fin in air according to the various lengths of fin and the various Rayleigh numbers. The experimental results are as follows; 1. When Rayleigh number is constant, local Nusselt number from the cylinder surface is decreased by increasing fin length. 2. When Rayleigh number is constant, mean fin Nusselt number has the highest value at ℓ=0.2 and the increasing value at ℓ<0.2 and the decreasing value at ℓ≥ 0.2 as the fin length increases. 3. Local fin Nusselt number is increased apparently near the base and rapidly near the tip, and the value near the tip is decreased as dimensionless fin length increases. 4. When fin length is constant, mean fin, mean cylinder and mean total Nusselt number are increased by increasing Rayleigh number.

정재하시험과 동재하시험에 의한 말뚝지지력 산정의 비교 연구 : 광주광역시 상무지구 현장 사례를 중심으로 하여

권호진,문보선 광주대학교 1996 産業技術硏究 Vol.7 No.-

본 연구에서는 말뚝 지지력 산정을 위한 정재하시험과 동재하시험에 대한 기본 이론과 특성을 살펴 보았고, 또한 문헌연구 및 현장실험을 통하여 두 시험간의 지지력을 비교 분석하여 동재하시험의 신뢰도에 대한 검토를 하였으며, 그 결과는 다음과 같다. (1) 현장실험1에서 동재하시험으로 구한 허용지지력은 59.37t이고, 정재하시험으로 구한 허용지지력은 62.50t 이상이었으며, 이는 기존의 연구와 비슷한 결과이다. (2) 현장실험2에서 정재하시험의 허용지지력은 65.00t 이상이고, 동재하시험에서는 시험항타시 편타에 의한 오차, 측정기기의 오차, 기술자의 특성에 따른 차이 등으로 시험결과의 CAPWAP분석이 불가능하여 지지력 산정에 실패하였다. (3) 동일말뚝에 대하여 동재하시험을 먼저 실시하고 정재하시험을 실시하면 정재하시험의 항복하중이 더 크게 나타날 수 있다. In this paper, the theory and characteristics of the static load test and the dynamic load test of pile were studied. And through the in-situ test in Sangmoo District of Kwangju the bearing capacity by two tests was compared and analyzed. The conclusions are followings. (1) The dynamic load test needs less time and less cost than the static load test. And in the dynamic load test, at any time during driving pile it is possible to measure the performance of pile and driving equipment, so it is possible to control reasonably the driving operation. (2) In case 1 of this study, the allowable bearing capacity of a pile from dynamic load test was 59.37 ton and that from static load test was above 62.50 ton. (3) In case 2 of this study, the allowable bearing capacity of a pile from static load test was above 65.0 ton, but the measurement from dynamic load test was impossible because of the eccentric hammer blow and the measuring equipment error and so on.

제지폐수처리용 생물고분자응집제를 생산하는 Bacillus sp. K-111의 배양특성조사

권기석,손용호,최선택,정석관,송숙희,김동걸 7개 국립대학교 환경연구 논문집 공동발행 위원회 2002 환경연구논문집 Vol.2 No.1

Flocculant-producing microorganisms were screened from obtained strains in the laboratory using a pulp-wastewater treatment as the flocculating agent. K-111 strain that had high flocculating activity among them was selected and identified as Bacillus sp. K-111 16s rDNA sequencing. The favorable medium for the production of flocculant was glucose 1.5%, NH_4NO_3 0.2%, tryptone 0.01%, K_2HPO_4 0.08%, KH_2PO_4 0.06%, CaCO_3 0.03%, MgSO_4·7H_2O 0.005%, MnSO_4 0.005% in 1 liter of D.W. at initial pH 7.0. The optimum culture temperature and pH were 30℃ and pH 7.0, respectively. the flocculating activity was observed most highly after 36 to 48 hr of cultivation at the optimum conditions. the flocculating activity of produced biopolymer on optimum conditions was about 2.5-fold higher than that of screening medium.

양극성장애환자의 정신사회적 스트레스에 관한 연구

권영준,한상우,한선호 순천향대학교 1990 논문집 Vol.13 No.1

The psychosocial stressors experienced in the 6 months prior to the admission were studied in 62(27 male and 35 female) inpatients who mets DSM-Ⅲ criteria for bipolar disorder and were hospitalized in the Neuropsychiatric department of Soonchunhyang University Hospital from January 1987 to August 1989. The results were as follows; 1. Among the 249 cases, 36(58.1%) cases had the psychosocial stressors prior to admission. 2. 40.7% of male cases and 71.4% of female cases had the psychosocial stressors prior to admission. Female cases had significantly higher relation to the psychosocial stressors. 3. By the age variable, there was not significant difference in relation to the psychosocial stressors prior to admission. 4. By the different educational level, there was not significant difference in relation to psychosocial stressors prior to admission. 5. By the occupation variable, there was not significant difference in relation to the psychosocial stressors prior to admission. 6. The frequency of the psychosocial stressors was in the order of occupational problems(36.1%), other interpersonal problems(22.1%), financial problems(11.1%) conjugal problems(11.1%).

정신분열병 환자의 인지기능에 대한 항정신병 약물의 영향에 관한 연구

권영준,한선호 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Objects : This study was performed to evaluate and compare the effects of classical and atypical antipsychotics on cognitive function of schizophrenic patients. Methods : 80 schizophrenic inpatients were selected for this study. They were divided into 4 groups and take one of the medication(haloperidol, risperidone, clozapine, and olanzapine). Their symptomatology, extrapyramidal side effects, and cognitive functions were measured at baseline and 6 weeks after medication by PANSS(Positive and Negative Syndrome Scale), ESRS(Extrapyramidal Symptom Rating Scale), Wechsler's memory scale, and Trail making test. Results : 1) The positive symptom, general psychopathology and total scores improved markedly, and there were no significant differences among 4 groups. The negative symtpom scores were improved better in risperidone, clozapine, and olanzapine groups than haloperidol group. 2) In ESRS, the questionnaire and parkinsonism scores were significantly elevated in all 4 groups, but the scores of haloperidol group were higher than other groups. The change of scores of dystonia and dyskinesia were not significant in risperidone, clozapine, and olanzapine groups, but in haloperidol group the scores were elevated significantly. 3) In the measures of cognitive functions, the logic memory were worsen in haloperidol, risperidone, and olanzapin groups, but not in clozapine group. The performance of forward digit span was preserved in atypical antipsychotics groups but not in haloperidol group. In the backward digit span and visual reproduction, haloperidol, risperidone, and olanzapine groups scored significantly lower than clozapine group. In the Trail making B, the performance time was increased markedly in haloperidol. and risperidone groups, but not in clozapine, olanzapine groups. Conclusion : Atypical antipsychotics effectively decreased negative symptoms, and have less extrapyramidal side effects compared with classical antipsychotics. Atypical neuroleptics, especially clozapine, seemed to preserve cognitive functions.