Characterizations of Ag-catalyzed ZnO nanostructures prepared by vapor–solid mechanism

Su-Hua Yang,Pao-Chih Chen,Sheng-Yu Hong 한국물리학회 2009 Current Applied Physics Vol.9 No.2

This paper presents the synthesis and field emission characteristics of zinc oxide (ZnO) nanostructures grown on Ag-deposited Si substrate by using the vapor–solid (VS) mechanism. The morphology of the ZnO nanostructures was related to source and substrate temperatures. Nanowhiskers, nanotips and well-aligned nanorods were grown at substrate temperatures of 620, 650 and 680℃, respectively. A strong bluish-green photoluminescence (PL) indicated that the nanostructures had many surface defects correlated with oxygen vacancies. Field emission analyses showed that the turn-on fields of ZnO nanowhiskers, nanotips and well-aligned nanorods were 4.5, 5.8 and 6.1 V/㎛, respectively. The high emitter density and high aspect ratio of the well-aligned nanorods resulted in an enhancement in emission current density. Furthermore, the field emission stability of the well-aligned nanorods was better than that of the nanowhiskers and nanotips. The emission current densities of the ZnO nanowhiskers, nanotips and well-aligned nanorods, measured after continuous operation for 6 h, were approximately 2.05, 4.68 and 20.5 μA/㎠, respectively. This paper presents the synthesis and field emission characteristics of zinc oxide (ZnO) nanostructures grown on Ag-deposited Si substrate by using the vapor–solid (VS) mechanism. The morphology of the ZnO nanostructures was related to source and substrate temperatures. Nanowhiskers, nanotips and well-aligned nanorods were grown at substrate temperatures of 620, 650 and 680℃, respectively. A strong bluish-green photoluminescence (PL) indicated that the nanostructures had many surface defects correlated with oxygen vacancies. Field emission analyses showed that the turn-on fields of ZnO nanowhiskers, nanotips and well-aligned nanorods were 4.5, 5.8 and 6.1 V/㎛, respectively. The high emitter density and high aspect ratio of the well-aligned nanorods resulted in an enhancement in emission current density. Furthermore, the field emission stability of the well-aligned nanorods was better than that of the nanowhiskers and nanotips. The emission current densities of the ZnO nanowhiskers, nanotips and well-aligned nanorods, measured after continuous operation for 6 h, were approximately 2.05, 4.68 and 20.5 μA/㎠, respectively.

Development and application of a UPLC method for studying infl uence of phenological stage on chemical composition of Scutellariae Radix

Hua Li,Hongjing Dong,Jianchun Su,Bin Yang 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.9

The influence of phenological stages onchemical composition of Scutellariae Radix (SR), theroot of Scutellaria baicalensis Georgi, was investigated. In order to deal with a large quantity of samples, arapid ultra-performance liquid chromatographic (UPLC)was first developed and validated for the simultaneousquantification of five flavonoids, namely baicalin(baicalein-7-O-b-D-glucuronide, BG), wogonoside(wogonin-7-O-b-D-glucuronide, WG), baicalein (BA),wogonin (WO), oroxylin A (OA) in the samples. Goodlinearity was obtained in the range of 0.742–389 ng(r2[0.9999) and satisfactory recoveries were obtained(101.72–104.56 %) with the RSD value below 5.0 %,for all analytes. Also, extraction conditions were optimizedto obtain maximum extractive contents of thefive flavonoids. Content variations of the five activeingredients in 225 samples from three different originswere investigated in five major phenological periods. Itwas found that the effect of phenology on the contentsof the tested five flavonoids was similar in the threeorigins. The contents of flavone O-glycosides, i.e., BGand WG accumulated to the highest level in leafexpansion period, while flavonoid aglycones, i.e., BA, WO and OA appeared a maximum concentration inflowering period. The UPLC method established in thisstudy was rapid and of good accuracy, repeatability andresolution, and hence can assist in the quality control ofSR.

Intervention Effects of Nedaplatin and Cisplatin on Proliferation and Apoptosis of Human Tumour Cells in Vitro

Su, Xiang-Yu,Yin, Hai-Tao,Li, Su-Yi,Huang, Xin-En,Tan, Hua-Yang,Dai, Hong-Yu,Shi, Fang-Fang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Objective: To study synergistic effects of nedaplatin and cisplatin on three human carcinoma cell lines (esophageal carcinoma cell line Eca-109, ovarian carcinoma Skov-3 and cervical carcinoma Hela). Methods: Inhibition effects were evaluated by MTT assay and cell apoptosis was detected by flow cytometry. In addition, changes of Ki-67, Bax and Bcl-2 at mRNA and protein levels were quantified by RT-PCR and Western blotting. Results: Growth inhibition in each cell lines was dose-dependent after exposure to nedaplatin or cisplatin alone. The interaction of the two drugs was synergistic at higher concentrations according to the median-effect principle. The inhibition rates with nedaplatin, cisplatin and combined treatment were $41.9{\pm}4.1%$, $47.4{\pm}2.9%$, $52.5{\pm}0.9%$(Eca-109), $39.0{\pm}1.26%$, $45.0{\pm}1.45%$, $56.2{\pm}1.44%$ (Skov-3) and $44.8{\pm}2.11%$, $46.9{\pm}0.99%$, $56.6{\pm}1.83%$ (Hela) respectively, with increase in apoptosis. Compared with the nedaplatin or cisplatin alone treatment group, the combinative treatment group's Ki-67 and bcl-2 mRNA (protein) expression was decreased while that of Bax mRNA (protein) was increased. Conclusion: Compared to the effects of nedaplatin or cisplatin alone at high concentrations, combination of nedaplatin and cisplatin at low concentrations proved to be much more effective for inhibition of proliferation and the induction of apoptosis in the Eca-109, Skov-3 and Hela cell lines.

The Crucial Role of the Establishment of Computed Tomography Density Conversion Tables for Treating Brain or Head/Neck Tumors

Yang, Shu-Chin,Lo, Su-Hua,Shie, Li-Tsuen,Lee, Sung-Wei,Ho, Sheng-Yow Korean Society of Medical Physics 2021 의학물리 Vol.32 No.3

Purpose: The relationship between computed tomography (CT) number and electron density (ED) has been investigated in previous studies. However, the role of these measures for guiding cancer treatment remains unclear. Methods: The CT number was plotted against ED for different imaging protocols. The CT number was imported into ED tables for the Pinnacle treatment planning system (TPS) and was used to determine the effect on dose calculations. Conversion tables for radiation dose calculations were generated and subsequently monitored using a dosimeter to determine the effect of different CT scanning protocols and treatment sites. These tables were used to retrospectively recalculate the radiation therapy plans for 41 patients after an incorrect scanning protocol was inadvertently used. The gamma index was further used to assess the dose distribution, percentage dose difference (DD), and distance-to-agreement (DTA). Results: For densities <1.1 g/cm³, the standard deviation of the CT number was ±0.6% and the greatest variation was noted for brain protocol conditions. For densities >1.1 g/cm³, the standard deviation of the CT number was ±21.2% and the greatest variation occurred for the tube voltage and head and neck (H&N) protocol conditions. These findings suggest that the factors most affecting the CT number are the tube voltage and treatment site (brain and H&N). Gamma index analyses for the 41 retrospective clinical cases, as well as brain metastases and H&N tumors, showed gamma passing rates >90% and <90% for the passing criterion of 2%/2 and 1%/1 mm, respectively. Conclusions: The CT protocol should be carefully decided for TPS. The correct protocol should be used for the corresponding TPS based on the treatment site because this especially affects the dose distribution for brain metastases and H&N tumor recognition. Such steps could help reduce systematic errors.

Anti-inflammatory activity of bark of Dioscorea batatas DECNE through the inhibition of iNOS and COX-2 expressions in RAW264.7 cells via NF-κB and ERK1/2 inactivation

( Mei Hua Jin ),( Seok Jong Suh ),( Ju Hye Yang ),( Yue Lu ),( Su Jeong Kim ),( Soon Youl Kwon ),( Tae Hyung Jo ),( Jin Wan Kim ),( Young In Park ),( Ghe Whan Ahn ),( Chong Kil Lee ),( Cheorl Ho Kim ) 영남대학교 약품개발연구소 2011 영남대학교 약품개발연구소 연구업적집 Vol.21 No.-

Identification of a Novel Frameshift Mutation in the ARSE Gene of a Newborn Boy Diagnosed with Chondrodysplasia Punctata 1 (CDPX1)

Pen Hua Su,Jia Yuh Chen,Ju Shan Yu,Suh Jen Chen,Jui Ming Hu,Jia Min Yang 한국유전학회 2007 Genes & Genomics Vol.29 No.3

X-linked chondrodysplasia punctata (CDPX 1) is a congenital disorder characterized by abnormalities in cartilage and bone development. Here, we examined a young, male subject diagnosed with chondrodysplasia punctata presented by typical radiologic findings of calcific stippling at the vertebrae as well as in the sacral area. CDPX 1 has long been thought to be a hereditary disease of generalized skeletal dysplasia. We discovered a de novo frame shift mutation in the arylsulphatase gene, ARSE, which generated an early stop codon. Remarkably, the mother of the newborn subject consumed Chinese herbs, cnidiir rhizome, during the entire pregnancy period. To the best of our knowledge, this is the first report of chondrodysplasia punctata that is correlated with mutations in exon 9 of the ARSE gene. Furthermore, we postulated that consuming specific herbs during prolonged periods while pregnant could be directly or indirectly related to the occurrence of mutation in the ARSE gene and may as a consequence lead to drug embryopathy.

Th2 cells infiltrating high-grade serous ovarian cancer: a feature that may account for the poor prognosis

Hao Su,Yueqi Jin,Changyu Tao,Hua Yang,Ence Yang,Wei-Guang Zhang,Fengzhi Feng 대한부인종양학회 2023 Journal of Gynecologic Oncology Vol.34 No.4

Objective: We aimed to investigate the differences of transcriptome profile between 2 groups of high-grade serous ovarian cancer (HGSOC) patients with distinct outcomes and identify potential biomarkers for recurrence. Methods: RNA sequencing was performed in 2 groups of HGSOC patients with similar demographic characteristics but exhibiting distinct progression-free survival (PFS). Transcriptome data of poor response (PR; PFS ≤6 months) and good response (GR; PFS ≥12 months) group were compared. We employed xCell to evaluate the abundance of 63 cells in tumor microenvironment. The predictive value of recurrence-related tumor infiltration cells was validated in cohort data from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) dataset. The weighted correlation network analysis was performed to identify the genes related to cell infiltration. Results: PR patients exhibited a distinct tumor infiltration immune cells-related transcriptional profile compared to GR patients, such as lower signatures of leukocyte differentiation, activation and chemotaxis. The fraction of T-helper 2 (Th2) cells infiltration was significantly higher in PR group than in GR group. High infiltration of Th2 was significantly associated with unfavorable prognosis in the GEO cohort (area under the curve=0.84 at 6 months recurrence) and TCGA cohort (p=0.008). Genes enriched to extracellular matrix organization and integrin binding were relevant to Th2 infiltration. Conclusion: Patients with HGSOC having shorter PFS exhibited a distinct gene signature that related to tumor-infiltrating immune cells. The level of Th2 infiltration could facilitate patient recurrence risk stratification and may be a promising biomarker for prognosis prediction and immune-related treatment.

Sofosbuvir/velpatasvir plus ribavirin for Child-Pugh B and Child-Pugh C hepatitis C virus-related cirrhosis

( Chen-hua Liu ),( Chi-yi Chen ),( Wei-wen Su ),( Chun-jen Liu ),( Ching-chu Lo ),( Ke-jhang Huang ),( Jyh-jou Chen ),( Kuo-chih Tseng ),( Chi-yang Chang ),( Cheng-yuan Peng ),( Yu-lueng Shih ),( Chia 대한간학회 2021 Clinical and Molecular Hepatology(대한간학회지) Vol.27 No.4

Background/Aims: Real-world studies assessing the effectiveness and safety of sofosbuvir/velpatasvir (SOF/VEL) plus ribavirin (RBV) for Child-Pugh B/C hepatitis C virus (HCV)-related cirrhosis are limited. Methods: We included 107 patients with Child-Pugh B/C HCV-related cirrhosis receiving SOF/VEL plus RBV for 12 weeks in Taiwan. The sustained virologic response rates at off-treatment week 12 (SVR₁₂) for the evaluable population (EP), modified EP, and per-protocol population (PP) were assessed. The safety profiles were reported. Results: The SVR₁₂ rates in the EP, modified EP and PP were 89.7% (95% confidence interval [CI], 82.5-94.2%), 94.1% (95% CI, 87.8-97.3%), and 100% (95% CI, 96.2-100%). Number of patients who failed to achieve SVR₁₂ were attributed to virologic failures. The SVR₁₂ rates were comparable regardless of patient characteristics. One patient discontinued treatment because of adverse events (AEs). Twenty-four patients had serious AEs and six died, but none were related to SOF/VEL or RBV. Among the 96 patients achieving SVR₁₂, 84.4% and 64.6% had improved Child-Pugh and model for endstage liver disease (MELD) scores. Multivariate analysis revealed that a baseline MELD score ≥15 was associated with an improved MELD score of ≥3 (odds ratio, 4.13; 95% CI, 1.16-14.71; P=0.02). Patients with chronic kidney disease (CKD) stage 1 had more significant estimated glomerular filtration rate declines than patients with CKD stage 2 (-0.42 mL/min/1.73 ㎡/month; P=0.01) or stage 3 (-0.56 mL/min/1.73 ㎡/month; P<0.001). Conclusions: SOF/VEL plus RBV for 12 weeks is efficacious and well-tolerated for Child-Pugh B/C HCV-related cirrhosis. (Clin Mol Hepatol 2021;27:575-588)

Aerosol delivery of urocanic acid-modified chitosan/programmed cell death 4 complex regulated apoptosis, cell cycle, and angiogenesis in lungs of K-ras null mice.

Jin, Hua,Kim, Tae Hee,Hwang, Soon-Kyung,Chang, Seung-Hee,Kim, Hyun Woo,Anderson, Hanjo K,Lee, Han-Woong,Lee, Kee-Ho,Colburn, Nancy H,Yang, Hsin-Sheng,Cho, Myung-Haing,Cho, Chong Su American Association for Cancer Research, Inc 2006 Molecular cancer therapeutics Vol.5 No.4

<P>The low efficiency of conventional therapies in achieving long-term survival of patients with lung cancer calls for development of novel treatment options. Although several genes have been investigated for their antitumor activities through gene delivery, problems surrounding the methods used, such as efficiency, specificity, and toxicity, hinder application of such therapies in clinical settings. Aerosol gene delivery as nonviral and noninvasive method for gene therapy may provide an alternative for a safer and more effective treatment for lung cancer. In this study, imidazole ring-containing urocanic acid-modified chitosan (UAC) designed in previous study was used as a gene carrier. The efficiency of UAC carrier in lungs was confirmed, and the potential effects of the programmed cell death protein 4 (PDCD4) tumor suppressor gene on three major pathways (apoptosis, cell cycle, and angiogenesis) were evaluated. Aerosol containing UAC/PDCD4 complexes was delivered into K-ras null lung cancer model mice through the nose-only inhalation system developed by our group. Delivered UAC/PDCD4 complex facilitated apoptosis, inhibited pathways important for cell proliferation, and efficiently suppressed pathways important for tumor angiogenesis. In summary, results obtained by Western blot analysis, immunohistochemistry, and terminal deoxynucleotidyl transferase-mediated nick end labeling assay suggest that our aerosol gene delivery technique is compatible with in vivo gene delivery and can be applied as a noninvasive gene therapy.</P>

Dynamic response analysis for structures with interval parameters

Chen, Su Huan,Lian, Hua Dong,Yang, Xiao Wei Techno-Press 2002 Structural Engineering and Mechanics, An Int'l Jou Vol.13 No.3

In this paper, a new method to solve the dynamic response problem for structures with interval parameters is presented. It is difficult to obtain all possible solutions with sharp bounds even an optimum scheme is adopted when there are many interval structural parameters. With the interval algorithm, the expressions of the interval stiffness matrix, damping matrix and mass matrices are developed. Based on the matrix perturbation theory and interval extension of function, the upper and lower bounds of dynamic response are obtained, while the sharp bounds are guaranteed by the interval operations. A numerical example, dynamic response analysis of a box cantilever beam, is given to illustrate the validity of the present method.