Human lactoferrin efficiently targeted into caprine beta-lactoglobulin locus with transcription activator-like effector nucleases

Yu-Guo Yuan,Shao-Zheng Song,Meng-Ming Zhu,Zheng-Yi He,Rui Lu,Ting Zhang,Fei Mi,Jin-Yu Wang,Yong Cheng 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.8

Objective: To create genetically modified goat as a biopharming source of recombinant human lacotoferrin (hLF) with transcription activator-like effector nucleases. Methods: TALENs and targeting vector were transferred into cultured fibroblasts to insert hLF cDNA in the goat beta-lactoglobulin (BLG) locus with homology-directed repair. The gene targeted efficiency was checked using sequencing and TE7I assay. The bi-allelic gene targeted colonies were isolated and confirmed with polymerase chain reaction, and used as donor cells for somatic cell nuclear transfer (SCNT). Results: The targeted efficiency for BLG gene was approximately 10%. Among 12 Bi-allelic gene targeted colonies, five were used in first round SCNT and 4 recipients (23%) were confirmed pregnant at 30 d. In second round SCNT, 7 (53%), 4 (31%), and 3 (23%) recipients were confirmed to be pregnant by ultrasound on 30 d, 60 d, and 90 d. Conclusion: This finding signifies the combined use of TALENs and SCNT can generate bi-allelic knock-in fibroblasts that can be cloned in a fetus. Therefore, it might lay the foundation for transgenic hLF goat generation and possible use of their mammary gland as a bioreactor for large-scale production of recombinant hLF.

Study on Multi Objective Optimization Method and It Application in Aviation and Deceleration Device

Yu Guang-bin,Song Ye,Duan Yuan-wang,Zhao Xing-fu,Qu Zhigang,Sun Yong-guo 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.5

Determination of equivalent blasting load considering millisecond delay effect

Song, Zhan-Ping,Li, Shi-Hao,Wang, Jun-Bao,Sun, Zhi-Yuan,Liu, Jing,Chang, Yu-Zhen Techno-Press 2018 Geomechanics & engineering Vol.15 No.2

In the analysis of the effects of rock tunnel blasting vibration on adjacent existing buildings, the model of simplified equivalent load produces higher calculation result of vibration, due to the lack of consideration of the millisecond delay effect. This paper, based on the static force equivalence principle of blasting load, proposes a new determination method of equivalent load of blasting vibration. The proposed method, based on the elastic-static force equivalence principle of stress wave, equals the blasting loads of several single blastholes in the same section of millisecond blasting to the triangle blasting load curve of the exploded equivalent elastic boundary surface. According to the attenuation law of stress wave, the attenuated equivalent triangle blasting load curve of the equivalent elastic boundary is applied on the tunnel excavation contour surface, obtaining the final applied equivalent load. Taking the millisecond delay time of different sections into account, the time-history curve of equivalent load of the whole section applied on the tunnel excavation contour surface can be obtained. Based on Sailing Tunnel with small spacing on Sanmenxia-Xichuan Expressway, an analysis on the blasting vibration response of the later and early stages of the tunnel construction is carried out through numerical simulation using the proposed equivalent load model considering millisecond delay effect and the simplified equivalent triangle load curve model respectively. The analysis of the numerical results comparing with the field monitoring ones shows that the calculation results obtained from the proposed equivalent load model are closer to the measured ones and more feasible.

Correspondence analysis for studying association between geography and cancer

Song, Joon-Jin,Yu, Pingjian,Ren, Yuan,Chung, Ming-Hua The Korean Data and Information Science Society 2009 한국데이터정보과학회지 Vol.20 No.5

Geographical location carries information such as demography, local economy, environment, and life styles, which could be the sources of cancer occurrence. Analyzing geographical location associated with cancer occurrence can be instructive to physicians, patients, and health administrators regarding resource allocation, expenditures, prophylaxis and treatments. In this paper, we explored the correspondence relationship between geographical locations and mortality rates of the cancers using correspondence analysis and illustrated the approach with the mortality rates of the top 10 cancers in the 75 counties in Arkansas from 2001 to 2005. Geographical variations with respect to the mortality rates of cancers are evaluated across Arkansas counties. Based on the contingency table, correspondence analysis model is developed and the simple indices which indicate the degree to which the regions and the cancers affect each other are calculated. Quantitative results are visualized and mapped in two-dimensional graphs.

Experimental Investigation and Finite Element Analysis on Flexural Behavior of PVA Fiber-Reinforced Recycled Concrete Slabs

Yuan Fang,Feng Yu,Jie Song,Yuandi Qian,Qinglin Tao 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.9

This paper presents an experimental study on mechanical behavior of Polyvinyl alcohol (PVA) fiber-recycled reinforced concrete (RC) one-way slabs. 21 specimens including 14 PVA fiber-reinforced recycled concrete slabs and 7 recycled RC slabs are fabricated and tested. The specimens are designed with different variables, such as replacement ratio of recycled coarse aggregate (RCA) γ, reinforcement ratio ρs, content of PVA fibers ρf, and span-to-thickness ratio l/h. The findings demonstrate that yielding of longitudinal reinforcement, breaking or pulling out PVA fibers along crack gaps and crushing of recycled concrete eventually dominate the failures of PVA fiber-reinforced recycled RC one-way slabs. The ultimate carrying capacity enhances as ρf or ρs increases, while decreases as l/h increases. The ultimate carrying capacity decreases with the increment of , while the introduction of PVA fibers can alleviate this weakening effect. The ultimate carrying capacity of RCB and PRCB decreases by 11.96% and 2.15%, respectively when the increases from 0% from 100%. The ultimate deflection first increases and then decreases as ρs increases. Increasing γ, l/h or ρf increases the ultimate deflection. Comparatively, the variation of PVA fiber content has a more prominent effect on the ultimate deflection of PRCB-U. The ultimate deflection of PRCB-U and PRCB increases by 73.03% and 15.15% respectively when the content of PVA fibers increases from 1% to 2%. A verified finite element (FE) model for PVA fiber-reinforced recycled RC one-way slabs under static load is established on account of rational constitutive relationship of materials. Then, the parametric studies are carried out to further reveal stress mechanism and the impacts of five main influence parameters on mechanical behaviors are ulteriorly analyzed. Ultimately, several design recommendations are suggested based on the experimental and FE analysis results. The suggested content of PVA fibers is between 0.5% and 2% and the optimal reinforcement rate ranges from 0.49% to 0.59%.

Effect of Light with Different Wavelengths on Nostoc flagelliforme Cells in Liquid Culture

( Yu Jie Dai ),( Jing Li ),( Shu Mei Wei ),( Nan Chen ),( Yu Peng Xiao ),( Zhi Lei Tan ),( Shi Ru Jia ),( Nan Nan Yuan ),( Ning Tan ),( Yi Jie Song ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.4

The effects of lights with different wavelengths on the growth and the yield of extracellular polysaccharides of Nostoc flagelliforme cells were investigated in a liquid cultivation. N. flagelliforme cells were cultured for 16 days in 500 ml conical flasks containing BG11 culture medium under 27 μmol·m-2·s-1 of light intensity and 25oC on a rotary shaker (140 rpm). The chlorophyll a, phycocyanin, allophycocyanin, and phycoerythrin contents in N. flagelliforme cells under the lights of different wavelengths were also measured. It was found that the cell biomass and the yield of polysaccharide changed with different wavelengths of light. The biomass and the yield of extracellular polysaccharides under the red or violet light were higher than those under other light colors. Chlorophyll a, phycocyanin, and allophycocyanin are the main pigments in N. flagelliforme cells. The results showed that N. flagelliforme, like other cyanobacteria, has the ability of adjusting the contents and relative ratio of its pigments with the light quality. As a conclusion, N. flagelliforme cells favor red and violet lights and perform the complementary chromatic adaptation ability to acclimate to the changes of the light quality in the environment.

Meta-analysis of the MDM2 T309G Polymorphism and Gastric Cancer Risk

Song, Bo,Duan, Zhong-Yu,Zhong, Yun-Hua,Lei, Na,Yang, Yu-Qing,Luo, Kai-Yuan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Background: Mdm2 binds to the amino-terminus of p53 to induce its degradation and a single nucleotide polymorphism in the MDM2 promoter region (T309G) has been reported to increase the risk of several carcinomas, such as gastric cancer. However, the results of published studies to analyze the association between MDM2 T309G and gastric cancer havve often conflicted. Methods: To better illustrate the filiation between MDM2 T309G and gastric cancer, we performed a meta-analysis. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to evaluate the strength of the relationship. The pooled ORs were performed for 4 models, additive, recessive, co-dominant model, and dominant. Results: Nine published case-control studies including 3,225 gastric cancer cases and 4,118 controls were identified. The MDM2 T309G polymorphism was associated with a significantly increased risk of gastric cancer risk when all studies were pooled into the meta-analysis (GG versus TT, OR=1.57; 95%CI=1.57-2.12; p=0.003) and GG versus GT/TT, OR=1.52; 95%CI=1.217-1.90; p<0.001). Furthermore, Egger's test did not show any evidence of publication bias (P = 0.608 for GG versus TT). Conclusion: Our results suggest that the MDM2 T309G polymorphism is indeed associated with a significantly increased risk of gastric cancer.

Enhanced Ultra‑violet Photodetection Based on a Heterojunction Consisted of ZnO Nanowires and Single‑Layer Graphene on Silicon Substrate

Yu Liu,Zengcai Song,Sheng Yuan,Lei Xu,Yanhui Xin,Meixia Duan,Shuxia Yao,Yangrui Yang,Zhenwei Xia 대한금속·재료학회 2020 ELECTRONIC MATERIALS LETTERS Vol.16 No.1

In this study, heterojunction photoelectric devices based ZnO nanowires were fabricated on p-Si substrate with and without single-layer graphene as insert layer. ZnO nanowires and graphene were prepared by hydrothermal method and chemical vapor deposition respectively. The efect of insert layer on the morphology of ZnO nanowires was very weak as can be seen from scanning electron microscope and X-ray difraction. Raman scattering showed that the graphene prepared was a singlelayer structure. The ultraviolet detection performance of photodetectors with single graphene insert layer was much better than that of photodetectors without single graphene insert layer. The ultraviolet irradiation sensitivity of photodetectors with single graphene insert layer was up to 1071 which was improved 7 times than that of photodetectors without single graphene insert layer. Moreover, photodetectors with single graphene insert layer had faster response time (1.02 s) and recovery time (0.34 s).

Nitrogen-doped hollow carbon spheres with highly graphitized mesoporous shell: Role of Fe for oxygen evolution reaction

Song, M.Y.,Yang, D.S.,Singh, K.P.,Yuan, J.,Yu, J.S. Elsevier 2016 Applied Catalysis B Vol.191 No.-

<P>There are many studies portraying iron (Fe) and nitrogen (N)-functionalzed carbon as an electrocatalyst along with possible elucidation of catalytically active sites. Despite continuous controversial debate on the active sites/species, the presence of N is believed to be undeniably needed for the efficient catalysis, whereas the necessity and role of Fe are still debated. To clearly understand the role of Fe in Fe and N-functionalized electrocatalyst, N-doped hollow mesoporous shell carbon (N-HMSC) is prepared as a uniform model electrocatalyst by a simple template nanocasting using Fe phthalocyanine (FePc) as a single precursor for carbon, N, and Fe. It is found that the presence of Fe in N-HMSC leads to the efficient graphitization of N-HMSC structure, which can be beneficial for electrocatalytic oxygen evolution reaction (OER). Interestingly, it is observed that Fe is a must for the preparation of high efficient catalyst, but may not be necessary for OER. (C) 2016 Elsevier B.V. All rights reserved.</P>

From Gene Map to Gene Expression Map: The Second Phase of the Chinese Superhybrid Rice Genome Project (SRGP)

Jun Yu,Song Nian Hu,Jun Wang,Gane K. S. Wong,Wei Lin,Xing Wang Deng,Li Huang Zhu,Meng Liang Cao,Long Ping Yuan,Jian Wang,Huan Ming Yang 한국육종학회 2002 한국육종학회 학술발표회 발표요지 Vol.34 No.2