Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

조선후기의 사회구조적 변화와 대원군의 정책

송이랑,김경호 東亞大學校 2000 東亞論叢 Vol.37 No.-

The purpose of this paper is to analyze and evaluate changes in social structure in the latter period of Chos?n and Daewongun's policies. During his rule, externally, the World Powers were eager to expand their imperialistic policies. Internally it was time for Chos?n to choose a new direction in history, largely due to the changes in the social structure. From an integrative point of view on structure and act rather than dualistic point, the paper examined the structure of ideology, status, agriculture, and commerce in the later Chos?n and then examined the relations between Chosen and Chung, between Chos?n and Japan, and between Chos?n and Western powers, respectively. Under such circumstance, in 1863, King Kojong ascended the throne Cheoljong and, immediately, Daewongun took the reigns of government. He tried to clean up deep-rooted evils caused by royal-married families(Sedojunchi), and to rule over the nation flexibly enough to accommodate various social changes. Accordingly, he promoted various policies in order to establish a new domestic order by way of overall social reforms. politically, Daewongun solidified his power basis and adopted merit system to allow more opportunities for the talent. His policies may be evaluated by three criteria - the will of ruler, support base, and the atmosphere of the times. Daewongun met two necessary conditions: leader's will on reform and possession of supporter groups. Nonetheless, his reformative policies can be evaluated as an half success because of premature social atmosphere to accommodate his reformative will. Thus, we come to conclusion that for the successive promotion of Daewongun's policies the three conditions must be worked out on mutual balance.

프린지 페스티벌의 효율적 운영방안에 관한 연구

김규현,이송이 경희대학교 디자인연구원 2005 예술· 디자인학연구 Vol.7 No.2

After the invention of a direct circuit in 1958 and since then we've came to live in the 21st Century's Information Industry Age called as the second Industrial Revolution. This direct circuit overlaps with so tiny circuit patterns within small silicon pieces that are undistinguishable seeing through human eyes as a result of bonding together with culture in discovering and inventing important all science (physics, chemistry, mathematics, optical science, electronic Engineering). This phenomenon has been set as human beings start to begin drawing such diagrams unintended' and those beautiful and intense images have been achieved as a result of technological development of Microchip diagrams' possessing exactness. Point and line's peculiar spacing characteristics, repetition, exactness and color are like a few best arts of our time that are enough to give more than artistic impression as the same power of splendid patterns that inspire and induce zest to us. This study discusses with the aesthetic beauties found in the electronic products' unseen precise functions aiming at logically and intuitionally planned in small high-tech chips' diagrams.

성역할, 의복동조성이 유행추구성향과 혁신추구성향에 미치는 영향

김연희;서민정;윤송이;이규혜 한양대학교 2006 韓國 生活 科學 硏究 Vol.26 No.1

Conformity and nonconformity in dress are the two driving forces that make diffusion of new styles. More fundamental personal attributes that affect individuality in dress are masculine or feminine sex roles. Researchers reported that little differences were found between men and women in terms of conformity values. Fashion and clothing products are related to hedonic shopping and major hedonic shopping orientations in clothing shopping behavior of college students were often reported. This study was aimed to empirically test the effect of personality traits of masculinity-femininity on nonconformity and conformity in dress; the effects of nonconformity and conformity on major hedonic shopping orientations. A survey questionnaire was developed for the empirical research. Data from 181 students were used for statistical analysis using structural equation modeling. A conceptual model with masculinity and femininity as two exogenous variables, two shopping orientations as endogenous variables and nonconformity-conformity as two mediating variables were analyzed. Results confirm the significant structural paths from masculinity to nonconformity, from femininity to conformity and from nonconformity to novelty-seeking and conformity to fashion seeking) were statistically significant. The results of this study suggested that The personality traits and shopping orientation were antecedents and consequences of nonconformity-conformity in dress and clearer relationships do exist in the flow.

北韓體制에 있어서 神話

송이랑,김경호 東亞大學校 2002 東亞論叢 Vol.39 No.-

This study is trying to detail things about North Korea through the legend of it. The legend is a kind of belief which is not based on the truth. The political legend is simply a fiction about things political. The system of North Korea has kept nervous since the beginning. As a result, it has focused on keeping the system rather than develops the country. This structural-character caused the legend. Especially the legend in the system of North Korea played the definite role in keeping the system of kim, IL-Sung and electing the successor.

보건교사의 비만관리실태 조사연구

김이순,주현옥,송미경 韓國學校保健學會 2002 韓國學校保健學會誌 Vol.15 No.2

This study was a research study to find out the real picture and practice of student obesity control by primary, middle and high school nurses. The study sampled 196 school nurses working in the Busan City and Northern Gyungsang Province, who agreed on the purpose of the study. When it comes to the study process, at the meeting of the whole school nurse, the researcher explained the purpose of the study and asked them to complete questionnaires and return on the spot. The study is designed to provide basic data for school nurses in field to systematically and efficiently control student obesity by examining perception of school obesity control and the real picture of practice of obesity control as well as its problems. The study used SPSS 10.0 to analyze data. The results are as follows: Annual plans about obesity control appeared significant at the primary school, 89.3%, higher than 77.3% of the middle and high school(p<0.05). Annual project evaluation showed a significant difference by regions, 56.0% of metro cities vs. small cities(p<0.01). The number of measuring weight of obese students was higher at the primary school than at the middle and high school(p<0.05). Also small cities conducted the weight measurement more often than metro cities(p<0.001). Among the health education methods for obesity, counseling appeared statistically significant(p<.01). The health education showed a significant difference in more experienced school nurses. Among the educational media, environment postings were used at the primary school, 24.0%, higher than 5.3% of the middle and high school. It showed a significant difference(p<.001). OHP or epidiascopes were used at the primary school, 36.4%, significantly higher than the middle and high school(p<.001). In addition, computer was used at the primary school, 21.5%, higher than the middle and high school(<.05). Problems of obesity education data included a lack of textbooks and programs and a short of educational media. However it didnt show a significant difference in schools or in regions. Counseling activities for obesity showed a significant difference in schools(p<.01) and in regions(p<.001). When parents with obese students were in counseling, those in their 20s used home correspondence and those in their 30s and 40s used phone call(p<.01). The rate of equipment to measure obesity was higher at the middle and high school than the primary school, showing a significant difference(p<.001). therefore, regular health education curriculums for school nurses should be systematically created to promote student health. Furthermore, it needs to make standard textbooks for health education by grades as well as by levels and to develop various educational programs and educational media.

수종의 불소방출 수복재의 탈회억제 효과 및 불소침투에 관한 연구

김송이,최성철,최영철,김광철,박재홍 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.3

자이오머(Giomer)는 레진강화형글라스아이오노머에 견줄 만큼 불소방출량이 많다고 하나, 우식예방 효과에 관한 연구들이 많지 않아 임상적용에 대한 근거가 부족한 실정이다. 본 연구는 자이오머(BF 군)를 포함하여 레진강화형글라스아이오노머(FF 군), 콤포머(DA 군)와 대조군인 콤포짓(FZ 군)의 우식억제 효과와 주변 치질로의 불소침투 양상을 비교하기 위하여, 공초점레이저주사현미경과 전자탐침미세분석기를 이용하여 각 수복재의 우식예방 효과를 비교하였으며, 다음과 같은 결과를 얻었다. 1. 수복물 주위 이차 인공우식에서 법랑질 외측병변의 깊이는 FZ 군에서 가장 깊었으며, BF 군이 가장 작게 나타났다(p<0.05). 2. 수복물과 법랑질 계면에 나타난 탈회억제층의 두께를 관찰한 결과 FZ 군이 가장 작게 나타났으며, FF, DA, BF 간에는 유의성 있는 차이를 보이지 않았다(p<0.05). 3. 법랑질에 침투된 불소농도는 BF, FF, DA의 순으로 높게 나타났다. 4. 상아질에 침투된 불소농도는 DA, BF, FF의 순으로 높게 나타났으며, 이는 상아질-수복물의 접착 계면에 영향 받는 것으로 보인다. The purpose of this study was to evaluate the effect of light cured fluoride-releasing materials on the inhibition of demineralization. In addition, the pattern of fluoride uptake of adjacent tooth structure was analyzed with EPMA. Eighty intact premolars were restored with Filtek Z250(control group, composite), Fuji Filling LC(RMGI), Dyract AP (compomer) and Beautifil II(giomer). Restored teeth were stored in distilled water for 30 days. Thensixty teeth(n=15) were exposed to demineralizing solution(pH 4.3). Demineralized teeth were bisected and polished. The specimens were observed with confocal laser scanning microscope. The depth of outer lesion and the thickness of inhibition zone were measured. Remained twenty teeth(n=5) were bisected for fluoride uptake analysis. The fluoride analysis were taken at enamel-restoration interface and dentin-restoration interface by electron probe micro-analyzer. The results are as follows: 1. The depth of outer lesion of Fuji Filling LC, Dyract AP, Beautifil II was shallower than that of Filtek Z250 at the margin of restoration(p<0.05). 2. The thickness of caries inhibition zone of Fuji Filling LC, Dyract AP, Beautifil II was greater than that of Filtek Z250 at the margin of restoration(p<0.05). 3. Fuji Filling LC, Dyract AP, Beautifil II groups showed the greater fluoride uptake into enamel and dentine around restoration than Filtek Z250 group. 4. In dentin the difference of fluoride concentration were greater than in enamel, and Dyract AP showed the greatest fluoride concentration in dentin.

하악 영구 견치의 의원성 매복에 대한 증례 보고

김송이,최성철,최영철,박재홍 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.2

상악 영구 견치의 매복에 비해 하악 영구 견치의 매복은 매우 드물게 일어난다. 치아가 매복된 경우 장애물 존재 여부와 매복 위치와 방향,맹출 가능한 공간의 유무,치근의 형성 단계 등의 요소에 따라 매복 치아의 발거 혹은 장애물의 제거 후 주기적 관찰,맹출이 지연된 치아의 외과적 노출,교정적 견인 등의 방법으로 치료하게 된다. 본 증례는 하악골의 골절 부위에 시행된 골간 강선 결찰과 미니 플레이트에 의해 하악 좌측 견치가 매복되어 있었던 경우로 CT 검사 결과 하악 정중부위 플레이트의 스크류가 맹출을 방해하고 있는 것으로 판단되어 전선 마취 하에 플레이트를 제거하였다. 이후 자연적인 맹출을 기대하였으나,맹출력이 부족하여 교정적 견인으로 양호한 결과를 얻었기에 보고하는 바이다. When a delayed eruption of tooth is diagnosed, the causative factor should be detected before an establishment of treatment plan, if possible. Although a panoramic radiograph is enough to evaluate the position of tooth and the stage of tooth development, a 3-D Dental CT would be a powerful tool to reveal a spatial relationships between objects. The reported case showed a delayed eruption of lower left permanent canine and a mini-plate with screws adjacent to the impacted canine. Although the screws adjacent to the root of impacted tooth showed a close proximity, it was not presumed that these screws would interfere the eruption of the tooth. The impacted canine did not show any spontaneous eruption during observation. After the mini-plate and screws were removed, an orthodontic traction using elastic power chain was performed. The position of mini-plates and screws should be carefully designed to avoid damaging the tooth follicles or tooth roots in the jaw. Also the screws should be removed before an orthodontic traction to prevent damaging the root surface of impacted tooth.

부유대역 용융응고법으로 제조된 YBa_2Cu_3O_x 산화물초전도체의 미세구조와 전기적 특성

노태형,송정환,장규철,한이섭,김소정,구본급,김호기 대전산업대학교 반도체기술연구소 2000 半導體技術硏究所報 Vol.2 No.-

The YBa_2Cu_3O_x-20 wt% Y_2BaCuO_5 composition designed in YBCO system was synthesized by solid state reaction method. The optimum condition of hot zone temperature and growth rate in floating-zone melt were 1080 ℃ and 2.5 mm/hour, respectively. In samples growth as these condition, the low angel boundaries in grain direction and small amount of amorphous phase known as decreasing characteristics of superconducting materials between grain boundaries in microstructure are investigated by polarized light microscopy on the whole. The critical current density(Jc) at 77k of sample using powder fabricated with no CeO_2 and doped 5 wt%CeO_2 were 8,000 A/㎠(Jc : 561A) and 12,000 A/㎠(Jc: 826A) at 0 T by direct current transport method, respectively.

대학생들의 자기이미지가 의복 혁신성과 동조성에 미치는 영향

서민정;김연희;윤송이;서문숙;이규혜 한양대학교 2008 韓國 生活 科學 硏究 Vol.28 No.1

The purpose of this study was to examine self images as antecedents of clothing innovativeness and conformity. A survey questionnaire was made for the empirical research. 206 university students living in Seoul metropolitan area participated in the study. The data were analyzed using factor analysis, Chi-square, t-test, ANOVA, and stepwise regression. The self image measures were classified six factors (free, affirmative, self-centered, traditional, sensual, and feminine image). According to regression analysis, fashion innovativeness was affected by free, traditional, sensual image, and conformity was only influenced by traditional image. Respondents were classified into four groups by innovativeness and conformity scores; Low innovativeness/Low conformity group(LI/LC), Low innovativeness/High conformity group(LI/HC), High innovativeness/Low conformity group(HI/LC), and High innovativeness/High conformity group(HI/HC). For LI/LC consumers, innovativeness was affacted by the sensual self image. For LI/HC consumers, and innovativenss was affected by the feminine self image. The conformity of HI/LC were influenced by traditional self image.