치수/치근단 질환에 이환된 영구치의 치수 조직 재생과 치근 형성

유연지,백승호,손호현 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.4

최근 치수 질환 또는 치근단 질환을 가진 미성숙 영구치에 대한 보존적 치료의 방법으로 여러가지 근관 내 소독 약제를 이용하에 증상 개선은 물론 치근의 성장 및 치수의 재생이 이루어진 증례들이 보고되고 있다. 그 기전에 대해서는 아직 명확하게 밝혀지지는 않았지만 여러가지 줄기 세포 또는 미분화 간엽 세포들이 관여하는 것으로 생각되며, 실제로 재생된 조직에서는 대부분백악질양또는골양물질의 침착이 관찰되고 있다. 이 새롭고보존적인 치료접근 방법은 다능성 줄기 세포와 다양한조직 공학 기술에 대한 연구와 더불어, 재생적 근관 치료에 더 밝은 비전을 제시하고 있다. Numerous cases about additional growth of roots or pulp tissue regeneration by using various intracanal medicaments in immature permanent teeth with periapical or pulpal disease have been reported. The underlying mechanism has not been clearly delineated. but it has been widely accepted that undifferentiated mesenchymal cells and stem cells are involved. Moreover, the growth and deposition of osteoid or cementoid tissues have been observed in regenerated pulp and roots. This new and non-invasive treatment has brightened the future of endodontics. and enlarged the vision of regenerative root canal treatment with multi-potent stem cells and various tissue engineering techniques.

The Effects of Panax ginseng and P. quinquefolium on Hemodynamics and Body Temperature in Healthy Young Men (3) (초록)

( Jee Hwan Lee ),( Jung Ah Cho ),( Chan Young Ki ),( Yeon Kyoung Son ),( Jeong Hill Park ),( Man Ki Park ),( Yong Nam Han ) 한국응용약물학회 2003 학술대회 Vol.2003 No.2

단순 한선 극세포종 1예

손호찬,이병진,임연순,한지윤 대한피부과학회 2003 大韓皮膚科學會誌 Vol.41 No.1

Hidroacanthoma simplex is a rare skin tumor which most often develops on the lower extremity of an elderly person. Clinically, the lesion appears as a well-demarcated brownish flat or verrucous plaque, resembling seborrheic keratosis or Bowen's disease. Histologically, it is characterized by intraepidermal nests of basaloid cells within an acanthotic epidermis. there is some controversy over the histogenesis of hidroacanthoma simplex, many consider it an epidermal tumor differentiation into intraepidermal eccrine duct cells. We report a case of hidroacanthoma simplex presented a small verrucous papule on the left foot with the results of an immunohistochemical study. (Korean J Dermatol 2003;41(1) 117∼120)

Estimation of Individual Leaf Area, Fresh Weight, and Dry Weight of Hydroponically-grown Ginseng (Panax Ginseng C.A.) Using Leaf Length, Width

Jee Hoon Kim,Min Young Kim,Tae Young Kim,Do Yeon Kim,Jung Eek Son 한국원예학회 2014 한국원예학회 학술발표요지 Vol.2014 No.5

Expression, purification, crystallization, and preliminary crystallographic analysis of the Fab fragment of golimumab, a therapeutic antibody against TNFα

Jee Un Lee,Ji Young Son,Woori Shin,Ki-Young Yoo,Heejin Lim,Ju Yeon Lee,Hyun Tae Lee,Yong-Seok Heo 한국구조생물학회 2017 Biodesign Vol.5 No.2

TNFα is an inflammatory cytokine required for immune responses, playing a crucial role in the pathogenesis of inflammatory autoimmune diseases via interactions with its cognate receptors, TNFR1 and TNFR2. Therapeutic antibodies targeting TNFα, including infliximab, adalimumab, certolizumab pegol, and golimumab, are clinically used for the treatment of the inflammatory diseases such as rheumatoid arthritis, psoriatic arthritis, Crohn’s disease, and inflammatory bowel disease. Here, the Fab fragment of golimumab was overexpressed in the periplasmic region of Escherichia coli and purified by affinity and gel-filtration chromatography. The purified protein was crystallized by hanging-drop vapor diffusion in the presence of 100 mM Tris, pH 8.5, 0.1 M ammonium phosphate, and 12% (w/v) PEG 6,000 at 20°C. The crystal diffracted X-ray to 2.60 Å resolution and belonged to the orthogonal space group P2 1 2 1 2, with unit cell parameters a = 72.74, b = 130.29, and c = 93.35 Å. An asymmetric unit of the crystal contains two molecules of the Fab fragment of golimumab with a V M of 2.14 Å 3 Da -1 and a solvent content of 42.48%.

신경행동평가의 단순 반응시간 검사에 시각 자극횟수 및 방법이 미치는 영향

손지연,김지성,이채용,이종영,김성아,남시현,박순우 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.3

컴퓨터를 이용한 단순반응시간 검사의 적정한 자극횟수를 정하고, 자극방법에 따른 단순반응시간의 차이를 보고자 교육수준과 연령이 동일한 층의 의과대학생들 240명을 대상으로 단순반응시간검사를 수행하였다. 적정자극횟수를 정하기 위해 120명에 대해 96회의 단순반응시간 검사를 수행하였다. 기존검사 및 연구에서 수행된 횟수를 포함하여 12회, 16회, 24회, 36회, 44회, 48회, 60회, 64회, 72회, 84회, 96회에 대해 평균과 표준오차 및 각각의 표준오차를 구하고 각각의 횟수에 대해 상관계수를 구하였다. 모든 횟수에서 남자 98명과 여자 22명간에 평균단순 반응시간은 통계적으로 유의한 차이를 보였으나(P<0.05), 표준편차는 차이를 보이지 않았다. 44회 이상부터는 비교적 안정된 값을 보였으며 남녀별로 비교적 안정된 값을 보인 횟수인 60회, 64회를 중심으로 상관분석 결과를 본 결과, 64회를 가장 적정한 자극횟수로 정하였다. 적정자극횟수로 선정한 64회로써 자극방법을 달리하여 검사한 후 상관분석한 결과에서도 자극횟수 64회의 적정성을 확인하였다. 자극방법에 따른 차이를 보기 위해서 또다른 120명에게 5x5cm의 적색사각형을 자극으로 준 단순반응시간 결과를 직경 2cm의 흰색원을 자극으로 주었던 앞서의 결과와 비교하였다. 적색 및 흰색 자극에 대해 각각 253.46 ±28.55msec, 269.31±26.90msec로서 통계적으로 유의한 차이를 보였다(p<0.01). 남녀별로도 동일한 결과를 보였다. 그러나, 표준편차에 대해서는 두군간, 남녀간 차이를 보이지 않았다. 컴퓨터를 이용한 검사에서 중요한 문제중의 하나인 동기유발방법에 따른 영향을 보고자 매 검사시행결과를 화면에 보여준 군과 보여주지 않은 군의 단순반응시간 검사결과를 비교하였다. 두군의 평균 단순반응시간은 각각 252.74±29.42 msec, 252.20±26.66 msec 로서 유의한 차이를 보이지는 않았으나 매시행 결과를 화면에 보여준 군에서 좀더 빠른 단순반응시간을 나타내었다. 표준편차에 대해서도 유의한 차이를 보이지 않았다. 따라서, 상이한 방법으로 수행된 신경행동검사결과의 비교시에는 자극방법에 따른 영향을 고려하여야 할 것이며 검사자의 동기유발을 위해 노력하여야할 것이다. In order to evaluate the effects of number and method of visual stimuli on simple reaction time and determine the proper number of trials, simple reaction time of the computerized test of NTOS(Neurobehavioral Test for Occupational Screening) was carried out on 240 medical students. The prominent difference was found between male and female on simple reaction time. Regarding the effect of number based on the parameters of measured and the purpose of test itself, 64 trials was considered as proper number. The mean reaction time was different according to the methods of visual stimuli, such as color and size(p<0.05). The larger and more intence visual stimuli were, the faster simple reaction time was. In respect to sex, that difference was similar. But, there was no difference depending upon whether the each result of each trial was showed on the monitor. The subjects, however, showed much interest when the result showed. Further studies, including the various variables on visual stimuli, are required to compare among various studies.

Effects of CoCl2 on Osteogenic Differentiation of Human Mesenchymal Stem Cells

Yeon-Hee Moon,Jung-Wan Son,Jung-Sun Moon,Jee-Hae Kang,Sun-Hun Kim,Min-Seok Kim The Korean Academy of Oral Biology 2013 International Journal of Oral Biology Vol.38 No.3

Objective. To investigate the effects of the hypoxia inducible factor-1 (HIF-1) activation–mimicking agent cobalt chloride (CoCl2) on the osteogenic differentiation of human mesenchymal stem cells (hMSCs) and elucidate the underlying molecular mechanisms. Study design. The dose and exposure periods for CoCl2 in hMSCs were optimized by cell viability assays. After confirmation of CoCl2-induced HIF-1α and vascular endothelial growth factor expression in these cells by RT-PCR, the effects of temporary preconditioning with CoCl2 on hMSC osteogenic differentiation were evaluated by RTPCR analysis of osteogenic gene expression, an alkaline phosphatase (ALP) activity assay and by alizarin red S staining. Results. Variable CoCl2 dosages (up to 500 μM) and exposure times (up to 7 days) on hMSC had little effect on hMSC survival. After CoCl2 treatment of hMSCs at 100 μM for 24 or 48 hours, followed by culture in osteogenic differentiating media, several osteogenic markers such as Runx-2, osteocalcin and osteopontin, bone sialoprotein mRNA expression level were found to be up-regulated. Moreover, ALP activity was increased in these treated cells in which an accelerated osteogenic capacity was also verified by alizarin red S staining. Conclusions. The osteogenic differentiation potential of hMSCs could be preserved and even enhanced by CoCl2 treatment.

영남지역 이모작 논에서의 보릿짚 처리 실태 조사

고지연 ( Jee Yeon Ko ),강항원 ( Hang Won Kang ),이재생 ( Jae Saeng Lee ),손일수 ( Il Soo Son ),김춘송,이동창 ( Dong Chang Lee ),김순철 ( Soon Chul Kim ) 한국토양비료학회 2003 한국토양비료학회 학술발표회 Vol.2003 No.-

OB07 Association between duration of antibiotic use and neonatal bronchopulmonary dysplasia in preterm premature rupture of membranes

( Chi-son Chang ),( Jin Ha Kim ),( Jee Youn Hong ),( Seo-yeon Kim ),( Ji-hee Sung ),( Suk-joo Choi ),( Soo-young Oh ),( Cheong-rae Roh ) 대한산부인과학회 2020 대한산부인과학회 학술대회 Vol.106 No.-

Objective: Although the use of broad-spectrum antibiotics in women with preterm premature rupture of membranes (PPROM) is recommended, the optimal duration of antibiotic use is unclear. Recent studies indicated that prolonged antibiotic exposure in very low birth weight neonates was associated with increased risk of bronchopulmonary dysplasia (BPD). However, the impact of duration of prenatal antibiotics exposure is rarely explored. The objective of this study was to investigate the effect of duration of antibiotic use in PPROM and compare the incidence of BPD. Methods: This retrospective study included 402 singleton pregnancies who were admitted to the high risk care unit of our institution due to PPROM between 20+0 and 33+6 week of gestation. We allocated the study population into three groups according to duration of antibiotic use; 7 days (group 1, n=259), 8-14 days (group 2, n=70), ≥ 15 days (group 3, n=73). The maternal baseline characteristics, delivery, and neonatal outcomes were compared among three groups. BPD was defined as a condition requiring oxygen for at least 28 days after birth, and severe BPD was defined as a condition requiring more than 30% oxygen and/or positive pressure at 36 week postmenstrual age or discharge, whichever comes first. Results: The gestational age at admission was lower in groups that used antibiotics longer (p < 0.001). The gestational age at delivery and birthweight showed no significant difference among three groups. Overall, the incidence of BPD and severe BPD was higher in groups that used antibiotics longer (BPD, 19.4% (48/247) in group 1 vs. 32.4% (22/68) in group 2 vs. 30.0% (21/70) in group 3, p = 0.023; severe BPD, 5.7% (14/247) in group 1 vs. 13.2% (9/68) in group 2 vs. 15.7% (11/70) in group 3, p = 0.004, by linear by linear association). Multivariate analysis also demonstrated that severe BPD showed incremental incidence according to longer duration of antibiotic use after adjustment for cofounders including gestational age at admission and delivery (OR [95% confidence interval], 2.17 [0.76-6.21] in group 2, 5.23 [1.45-18.89] in group 3 compared to group 1 as a reference). Conclusion: Our data demonstrated that longer antibiotic use (≥ 15 days) in PPROM was associated with higher risk for severe BPD.

Trichophyton meontagrophytes에 의한 안면부 잠행 백선 1 예

한지윤,임연순,손호찬,강현영,조윤화 대한피부과학회 2000 대한피부과학회지 Vol.38 No.8

Recently, we have encountered an increasing number of patients who have obtained topical steroids to self-treat various dermatosis. Tinea incognito has been used to describe a dermatophyte infection modified by corticosteroid treatment. Lesions are often atypical appearing and the diagnosis can be delayed or missed. We report a case of tinea incognito with ill-defined erythematous patch and nodules on the right upper eyelid and taken intermittently application with corticosteroid ointment over a period of 1 year. Mycologic studies including KOH mount and fungus culture were positive for hyphae and colonies of Trichophyton mentagrophytes. The lesion was treated with of itraconazole and cured 3 weeks later.