Prevalence of Feline Leukemia Virus Infection in Cats in Bangladesh

Siddiqur Rahman,Salauddin Bhuiyan,Taohidul Islam,Azimun Nahar,Roma Rani Sarker,Emtiaj Alam,Amitavo Chakrabarty,Abu Sayed Sarker,Laila Akhter,채준석 한국임상수의학회 2014 한국임상수의학회지 Vol.31 No.1

Feline leukemia virus (FeLV) is a retrovirus that represents one of the most common and important infectiousdiseases of cats worldwide and it is responsible for more deaths among cats than any other infectious diseases. Prevalence data are necessary to define prophylactic, management and therapeutic measures for stray, feral and ownedcats which are lacking in Bangladesh. The study was carried out to determine the prevalence of FeLV infection inMymensingh district of Bangladesh using RapiGEN® FeLV Ag Test Kit (RapiGEN® Inc., Republic of Korea), a rapidone-step immunochromatographic assay. Blood samples from total 130 cats (23 owned cats and 107 unowned cats)were collected and tested following the manufacturer’s instruction. An overall prevalence of FeLV infection was 1.54%(2/130). Prevalence was found 1.79% (2/112) on Day 0-up to one year aged cats (young) but no positive case wasfound in above 1 year (Adult) aged group. In male and female cats, the prevalence was 1.72% (1/58) and 1.39%(1/72), respectively. In un-owned cats the prevalence was 1.87%. Positive cases to FeLV were found only in clinicallysick cats. No significant relationship was found according to age, sex, ownership status and health status. To the bestof our knowledge this is the first report of the prevalence of FeLV infection in Bangladesh using RapiGEN® FeLVtest kits which is very much effective because it is easy to apply, less expensive and quick screening of such typeof infection.

Forest and agro-ecosystem productivity in Bangladesh: a climate vegetation productivity approach

Md. Siddiqur Rahman,Salena Akter,Mohammed Al-Amin 한국산림과학회 2015 Forest Science And Technology Vol.11 No.3

The aim of the study was to assess spatial and temporal variation in productivity with respect to climate factors in Bangladesh in different forest ecosystems and agro-ecological zones. A climate vegetation, and productivity index (CVPI) for different vegetation types of Bangladesh were measured for years ranging between 1990 and 2010. Data were gathered from 11 meteorological stations sporadically distributed throughout the country. The range of CVPI at different vegetation zones of the country shows values between 1223 and 2800 (this index has no unit). Spatial distribution of values indicates that CVPI is lower in northwestern and southwestern agro-ecological zones of the country, whereas it is higher in eastern zones. This may be due to less rainfall and higher atmospheric temperature in the western part than the eastern part. CVPI in the central part of Bangladesh also decreases while the temporal scenario also varies significantly. There was a peak in the index during the year 1998 when the country faced extreme precipitation followed by devastating floods. Both spatial and temporal variation depicts that vegetation productivity would increase or decrease with respect to climatic parameters such as mean monthly temperature, precipitation, and solar radiation received at the site. For major types of forested woodland, tropical evergreen and semi-evergreen types cover the range of 21502800, moist Sal forest covers 12231896, dry Sal forest covers 12771280, Sundarbans covers 1307, and coastal plantations cover 19462531 CVPI. This value greatly depends on the spatial coordinates of the meteorological stations. From Paterson’s regression, forest timber productivity was calculated which was found to be higher for evergreen, semi-evergreen, and coastal plantation (about 1011 m3 ha ¡1 yr ¡1), where deciduous Sal and Sundarbans has lower productivity (about 89 m3 ha ¡1 yr ¡1) than hill forests. Thus, climatic factors, as well as altitudinal and latitudinal differences, may pose divergence in forest productivity. Hence, climate is the key factor in forest productivity and distribution.

Short Communications : Seroprevalence of brucellosis in cattle in selected area of Bangladesh and comparison between Rose Bengal test and i-ELISA used for the screening of brucellosis

( Md. Siddiqur Rahman ),( Amitavo Chakrabartty ),( Taohidul Islam ),( Roma Rani Sarker ),( Me Alam1,Muhammad Jasim Uddin ),( Laila Akther ),( Hee Jong Song ) 한국가축위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.2

Brucellosis, a bacterial zoonoses caused by the genus Brucella is responsible for abortion and infertility in cow. Brucellosis is causing economic loss in dairy industries and prevalent worldwide including Bangladesh but limited studies are devoted to determine the prevalence and its association with reproductive factors of dairy cows in Bangladesh. Therefore, the present study was conducted to determine the seroprevalence of brucellosis in dairy cattle using screening test Rose Bengal test (RBT) and the positive sera were further confirmed by indirect- ELISA. For this purpose, a total of 400 serum samples from dairy cows with history of abortion and various reproductive disorders were collected from the Kurigram district of Bangladesh for the detection of Brucella antibody. The overall prevalence of brucellosis in dairy cattle was 2.25%. Brucellosis in cases of abortion and repeat breeding was 8.3% and 2.8%, respectively. The results shows higher prevalence of brucellosis in cases of abortion followed by repeat breeding, while there was no seropositive cases from other reproductive disorders. Age-wise seroprevalence was found 3.0% in 2∼3 years age group and 2.0% in 4∼8 years age group. The prevalence of brucellosis in indigenous and cross-bred cattle was 3.6% and 1.7%, respectively. All the animals detected positive to brucellosis by RBT were not found to be positive by i-ELISA. However, the RBT might be a suitable screening test for the diagnosis of Brucella infection in field condition in Bangladesh. These data will help to develop effective disease prevention strategies.

Infection of Brucella abortus Biotype 1 to Pregnant Sprague-Dawley Rat

Baek, Byeong Kirl,Siddiqur, Rahman Mohammad,Kiku, Mastuda,Lee, JooYoung,Hur, Jin,Lim, Chai Woong,Ibulaimu, Kakoma 한국수의공중보건학회 2001 예방수의학회지 Vol.25 No.4

한우에서 분리한 Brucella abortus biotype 1의 1×10^9cfu 균수를 임신 경험이 있는 암쥐와 임신 경험이 없는 처녀 쥐에게 임신 7일, 14일 그리고 18일째 각각 경피 감염시켰다. 인공 감염된 군은 간, 비장 조직에서 AMOS PCR과 조직의 세균 배양 시험으로 균체를 확인하였다. 감염 쥐의 체온은 최고 38℃까지 상승하였으나 대조군에서는 상승하지 않았다. 감염시킨 군에서는 사산, 유산, 조산, 체온의 재 상승 등이 없었으나 비장확대, 자궁염, 림프절의 종대, 태반염과 염증 증상이 육안적으로 관찰되었다. 본 연구를 통하여 Brucella 연구의 모델로서 쥐의 사용 가능성과 브루셀라 보균체로서의 역할 가능성을 있음을 알 수 있었다.

New Sources of Resistance to Cucumber mosaic virus in Capsicum annuum

Md. Siddiqur Rahman,Abdul Mannan Akanda,Ismail Hossain Mian,Md. Khurshed Alam Bhuiyan,Md. Motaher Hossain 한국작물학회 2016 Journal of crop science and biotechnology Vol.19 No.3

Cucumber mosaic virus (CMV) is the most serious virus disease affecting chilli (Capsicum annuum L.) worldwide and the absence of natural resistance makes management of CMV outbreaks difficult. The characterization of improved sources of resistance to CMV in chilli would facilitate the development of commercially acceptable chilli varieties with adequate levels of CMV resistance. A total of 30 chilli genotypes were evaluated for their reaction to CMV in field and artificial inoculated conditions during 2010-2011 and 2011-2012. Large differences were observed among genotypes for disease incidence, severity indexes, and yield losses. Based on observed data, genotype CA23 (Noakhali) was identified as resistant, while CA12 (Comilla-2) was categorized as moderately resistant to CMV both in natural and inoculated conditions. Enzyme-linked immunosorbent assay absorbance values of samples taken from CMV-infected leaves corresponded well with visible viral symptoms for these genotypes. The identified C. annuum CA23 and CA12 genotypes represent previously undescribed and potentially useful sources of CMV resistance.

Artocarpus chaplasha: Establishment and Initial Growth Performance at Elevated Temperature and Saline Stresses

Md. Siddiqur Rahman,M. Al-Amin,Salena Akter 강원대학교 산림과학연구소 2012 Journal of Forest Science Vol.28 No.1

Like any other natural resources, forest flora may experience the extreme threat of elevated temperature and saline water submergence at different stages of their lives i.e. from germination to maturity due to climate change effects. The overall aim of the study was to measure the effect of higher temperatures along with saline water irrigation on survival and initial growth during seedling stage of Artocarpus chapalasha. The experiment was conducted in temperature-humidity-photoperiod regulated plant growth chamber during stipulated period to measure the growth performance of randomly selected seedlings. Within three different elevated temperatures viz. 30oC, 32oC and 34oC, the seedlings were given three different saline conditions such as 0.5 g/L, 1.5 g/L and 2.5 g/L NaCl concentrations. Results found from the experiment was that, seedlings of Artocarpus chaplasha reared at different temperatures and saline water treatments showed stunted growth than reared at existing outdoor temperature (26.31°C) irrigated with regular fresh water. Seedling growth at three different parameters such as height, collar diameter and number of leaves showed that with increasing temperature individuals respond negatively to increasing saline condition. The seedling’s growth occurred at every day in height, collar diameter and leaf. However, growth rate reduced later during the observation. The combined effect of high salinity and higher elevated temperature results in seedling mortality. Therefore, Artocarpus chaplasha may not thrive at higher temperature and salinity intrusion at its early growing period in plantation and natural forest areas.

Characterization of a New Isolate of Chilli ringspot virus in Yunnan, China

Md. Siddiqur Rahman,Xiaoxia Su,Kuanyu Zheng,Xiaofei Cheng,Ting Li,Lihua Zhao,Jiahong Dong,Zhongkai Zhang 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.1

Virus diseases are the major limiting factors of chilli production in China. A virus isolate of chilli ringspot virus-Yunnan (ChiRSV-YN) from chilli (Capsicum annuum L.) showing mild mosaic, leaf crinkling, ringspot, and vein banding symptoms was recently identified in Yunnan, China. The electron microscopy confirmed the virus as a typical potyvirus having flexuous filamentous particles ca. 780 nm in length. Several chilli and tobacco varieties were tested through mechanical inoculation and found to be susceptible to the virus isolate ChiRSV-YN. The viral genome was sequenced through RNA sequencing. The complete genomic RNA of the isolate (GenBank Acc. No. KX258620) consisted of 9,652 nucleotides (nt) excluding the poly (A) tail at the 3ʹ end. The isolate contains a large ORF which encodes a polyprotein of 3,086 amino acids (aa) with Mr. of 349.21 kDa. It had a typical genomic organization of potyviruses. The complete nucleotide and polyprotein sequences of ChiRSV-YN had 87.6 and 93.2% identities with the ChiRSV-HN/14, respectively. The result showed ChiRSV-YN is a new isolate of ChiRSV and it is the first full-length genome characterization of ChiRSV in Yunnan, China. Understanding of the characteristics of this virus isolate might provide the basis for disease diagnosis and control in the future.

Short Communication : Prevalence of brucellosis and its association with reproductive problems in goats in Bangladesh

( Md Siddiqur Rahman ),( Muhammad Jasim Uddin ),( Hee Jong Song ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.3

Short communication : Tube agglutination test is superior than other serological tests for diagnosis of brucellosis in small ruminants

( Md. Siddiqur Rahman ),( Nusrat Jahan ),( Mohammad Arif Hossain ),( M. J. Uddin ),( Niraj Kanti Shil ),( KBM Saiful Islam ),( Md. Shamim Ahasan ),( A. K. M. Anisur Rahman ),( Hee Jong Song ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.4

Brucella spp. are small, non-motile Gram-negative coccobacilli known to cause disease in a number of vertebrate species including humans and brucellosis is one of the world`s major zoonoses, alongside bovine tuberculosis and rabies. There are about 33.55 million goats and 1.16 million sheep in Bangladesh. The sheep and goats can significantly play an important role in the economic well being of the resource-poor farmer in Bangladesh. Sexually matured 362 female small ruminants (300 goats and 62 sheep) were examined. Approximately 3-5ml of blood was collected from the jugular vein of each animal and sera samples were prepared. Samples were then tested for brucellosis by using Rose Bengal test(RBT), plate agglutination test(PAT) and tube agglutination test(TAT). Among 362 small ruminants, irrespective of species(sheep or goat), diagnosed highest in TAT, 2.21%(n=8) and lowest both by RBT & PAT, 1.93%(n=7) and it is concluded that TAT is superior than RBT and PAT.

Indirect enzyme Linked immunosorbent assay for the diagnosis of brucellosis in cattle

( Md. Siddiqur Rahman ),( Md. Fazlul Huque ),( Md. Shamim Ahasan ),( Hee Jong Song ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.2

Brucellosis is a major zoonosis caused by Gram negative facultative intracellular bacterial organisms of the genus Brucella that are pathogenic for a wide variety of animals and human beings. Because of its economic impact on animal health and the risk to the human population,most countries have a brucellosis control program. Brucellosis is also an economically important andprevalent disease in Bangladesh. The accurate and prompt diagnosis is very important in controlling and eradicating of the disease in animals. The present study was undertaken to determine the seroprevalence of brucellosis in cattle in Mymensingh and Patuakhali district of Bangladesh. A total of 120 serum samples were collected from the two districts along with a questionnaire related to the epidemiology of the disease. The sampleswere screened by using slow agglutination test and conformed by indirect enzyme linked immunosorbent assay. The overall seroprevalence of brucellosis in cattle was 5% and it was observed that, a higher prevalence of Brucella was found in female than male, through natural breeding than artificial insemination (AI) and animal above 4 years old are highly susceptible than younger ones. Higher prevalence was found in aborted animals in comparison with non aborted animal. Finally, the study revealed that the female animal has more susceptible to brucellosis and healthy semen should be used for AI.