Isolation and identification of infectious bursal disease virus from broiler and layer chickens during the outbreak year 2007 in Bangladesh

Islam, Md. Taohidul,Mohiuddin, Mohammad,Hossain, Muhammad Tofazzal,Rahman, Md. Bahanur,Rahman, Md. Mostafizur,Rahman, Md. Siddiqur,Song, Hee-Jong,Islam, Md. Alimul The Korean Society of Veterinary Service 2012 韓國家畜衛生學會誌 Vol.35 No.1

The objective of the present study was to isolate and identify infectious bursal disease viruses (IBDVs) from broiler and layer chickens of outbreaks of infectious bursal disease (IBD) in three districts of Bangladesh. A total of 70 bursal samples were collected from dead broiler (n=40) and layer (n=30) chickens showing specific lesions of IBD from seven commercial poultry farms of three different districts (Mymensingh, Chittagong and Tangail) of Bangladesh during the year 2007. Five representative bursal samples from each farm were used for the isolation of IBDVs using 9-day-old embryonated eggs of seronegative flock of layer birds and for identification the samples were subjected to agar gel immunodiffusion test (AGIDT), immunohistochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR). Out of 35 bursal samples, IBDVs were successfully isolated from 28 (80%) samples. By AGIDT, 32 (91.4%) samples were found positive for IBDV antigen. Results of AGIDT clearly indicated that IBDVs detected in 29 bursal samples of six affected farms were identical to each other but not to IBDVs present in the remaining three samples of another farm. Indirect immunoperoxidase staining of the bursal sections revealed the presence of IBDV antigen in 32 (91.4%) samples and the IBDV antigen was detected mainly in the cortex of the lymphoid follicles of the bursal tissues. In histopathology, cell depletion, atrophy and necrosis were observed in many bursal follicles with severe edema of interfollicular septa. Of the 35 bursal samples, 34 (97.1%) samples generated 254 bp product by RT-PCR. In conclusion, the results of virus isolation and identification by AGIDT, IHC and the analysis of viral genome by RT-PCR confirmed the outbreaks of acute IBD in commercial poultry of Bangladesh. Moreover, histopathological findings and results of AGIDT gave a clear indication that the isolates from six outbreaks were different from classical strain and it seems to be of very virulent strain. On the other hand, the isolates from the other outbreak were similar to the classical strain.

Isolation and identification of infectious bursal disease virus from broiler and Layer chickens during the outbreak year 2007 in Bangladesh

( Taohidul Islam ),( Mohammad Mohiuddin ),( Muhammad Tofazzal Hossain ),( Bahanur Rahman ),( Mostafizur Rahman ),( Siddiqur Rahman ),( Hee Jong Song ),( Alimul Islam ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.1

The objective of the present study was to isolate and identify infectious bursal disease viruses (IBDVs) from broiler and layer chickens of outbreaks of infectious bursal disease (IBD) in three districts of Bangladesh. A total of 70 bursal samples were collected from dead broiler (n=40) and layer (n=30) chickens showing specific lesions of IBD from seven commercial poultry farms of three different districts (Mymensingh, Chittagong and Tangail) of Bangladesh during the year 2007. Five representative bursal samples from each farm were used for the isolation of IBDVs using 9-day-old embryonated eggs of seronegative flock of layer birds and for identification the samples were subjected to agar gel immunodiffusion test (AGIDT), immunohistochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR). Out of 35 bursal samples, IBDVs were successfully isolated from 28 (80%) samples. By AGIDT, 32 (91.4%) samples were found positive for IBDV antigen. Results of AGIDT clearly indicated that IBDVs detected in 29 bursal samples of six affected farms were identical to each other but not to IBDVs present in the remaining three samples of another farm. Indirect immunoperoxidase staining of the bursal sections revealed the presence of IBDV antigen in 32 (91.4%) samples and the IBDV antigen was detected mainly in the cortex of the lymphoid follicles of the bursal tissues. In histopathology, cell depletion, atrophy and necrosis were observed in many bursal follicles with severe edema of interfollicular septa. Of the 35 bursal samples, 34 (97.1%) samples generated 254 bp product by RT-PCR. In conclusion, the results of virus isolation and identification by AGIDT, IHC and the analysis of viral genome by RT-PCR confirmed the outbreaks of acute IBD in commercial poultry of Bangladesh. Moreover, histopathological findings and results of AGIDT gave a clear indication that the isolates from six outbreaks were different from classical strain and it seems to be of very virulent strain. On the other hand, the isolates from the other outbreak were similar to the classical strain.

Molecular characterization of two Bangladeshi infectious bursal disease virus isolates using the hypervariable sequence of VP2 as a genetic marker

Md Taohidul Islam,Thanh Hoa Le,Md. Mostafizur Rahman,Md. Alimul Islam 대한수의학회 2012 JOURNAL OF VETERINARY SCIENCE Vol.13 No.4

Two Bangladeshi infectious bursal disease virus (IBDV) isolates collected in 2007, termed GB1 and GB3, were subjected to comparative sequencing and phylogenetic analyses. Sequence analysis of a 474-bp hypervariable region in the VP2 gene revealed that among four major amino acid substitutions observed in the strains, two were unique to GB1 and GB3 (Ser217Leu and Ala270Thr) while one substitution was only found in GB1 (Asn299Ser). Among IBDVs from Bangladesh including GB1 and GB3, the rate of identity and homology was around 97∼99%. The amino acid sequences of GB1 and GB3differ from those of previous Bangladeshi IBDV isolates and contain amino acid substitutions Pro222Ala and Asn299Ser (in GB3 only). Phylogenetic analysis revealed that GB1 and GB3 are grouped with other very virulent IBDVs of European and American origin in contrast to two previously isolated Bangladeshi IBDV strains (GenBank accession Nos. AF362776 and AF260317), which belong to the Asian group. It was concluded that GB1 and GB3 belong to a very virulent group of IBDVs. However, amino acid sequences of GB1 and GB3 differ from those of the other Bangladeshi IBDVs by one or two amino acids encoded in the hypervariable region of the VP2 gene.

Prevalence of Feline Leukemia Virus Infection in Cats in Bangladesh

Siddiqur Rahman,Salauddin Bhuiyan,Taohidul Islam,Azimun Nahar,Roma Rani Sarker,Emtiaj Alam,Amitavo Chakrabarty,Abu Sayed Sarker,Laila Akhter,채준석 한국임상수의학회 2014 한국임상수의학회지 Vol.31 No.1

Feline leukemia virus (FeLV) is a retrovirus that represents one of the most common and important infectiousdiseases of cats worldwide and it is responsible for more deaths among cats than any other infectious diseases. Prevalence data are necessary to define prophylactic, management and therapeutic measures for stray, feral and ownedcats which are lacking in Bangladesh. The study was carried out to determine the prevalence of FeLV infection inMymensingh district of Bangladesh using RapiGEN® FeLV Ag Test Kit (RapiGEN® Inc., Republic of Korea), a rapidone-step immunochromatographic assay. Blood samples from total 130 cats (23 owned cats and 107 unowned cats)were collected and tested following the manufacturer’s instruction. An overall prevalence of FeLV infection was 1.54%(2/130). Prevalence was found 1.79% (2/112) on Day 0-up to one year aged cats (young) but no positive case wasfound in above 1 year (Adult) aged group. In male and female cats, the prevalence was 1.72% (1/58) and 1.39%(1/72), respectively. In un-owned cats the prevalence was 1.87%. Positive cases to FeLV were found only in clinicallysick cats. No significant relationship was found according to age, sex, ownership status and health status. To the bestof our knowledge this is the first report of the prevalence of FeLV infection in Bangladesh using RapiGEN® FeLVtest kits which is very much effective because it is easy to apply, less expensive and quick screening of such typeof infection.

Seroprevalence of brucellosis in cattle in selected area of Bangladesh and comparison between Rose Bengal test and i-ELISA used for the screening of brucellosis

Rahman, Md. Siddiqur,Chakrabartty, Amitavo,Islam, Md. Taohidul,Sarker, Roma Rani,Alam, M.E.,Uddin, Muhammad Jasim,Akther, Laila,Song, Hee-Jong The Korean Society of Veterinary Service 2012 韓國家畜衛生學會誌 Vol.35 No.2

Brucellosis, a bacterial zoonoses caused by the genus Brucella is responsible for abortion and infertility in cow. Brucellosis is causing economic loss in dairy industries and prevalent worldwide including Bangladesh but limited studies are devoted to determine the prevalence and its association with reproductive factors of dairy cows in Bangladesh. Therefore, the present study was conducted to determine the seroprevalence of brucellosis in dairy cattle using screening test Rose Bengal test (RBT) and the positive sera were further confirmed by indirect- ELISA. For this purpose, a total of 400 serum samples from dairy cows with history of abortion and various reproductive disorders were collected from the Kurigram district of Bangladesh for the detection of Brucella antibody. The overall prevalence of brucellosis in dairy cattle was 2.25%. Brucellosis in cases of abortion and repeat breeding was 8.3% and 2.8%, respectively. The results shows higher prevalence of brucellosis in cases of abortion followed by repeat breeding, while there was no seropositive cases from other reproductive disorders. Age-wise sero-prevalence was found 3.0% in 2~3 years age group and 2.0% in 4~8 years age group. The prevalence of brucellosis in indigenous and cross-bred cattle was 3.6% and 1.7%, respectively. All the animals detected positive to brucellosis by RBT were not found to be positive by i-ELISA. However, the RBT might be a suitable screening test for the diagnosis of Brucella infection in field condition in Bangladesh. These data will help to develop effective disease prevention strategies.

Short Communications : Seroprevalence of brucellosis in cattle in selected area of Bangladesh and comparison between Rose Bengal test and i-ELISA used for the screening of brucellosis

( Md. Siddiqur Rahman ),( Amitavo Chakrabartty ),( Taohidul Islam ),( Roma Rani Sarker ),( Me Alam1,Muhammad Jasim Uddin ),( Laila Akther ),( Hee Jong Song ) 한국가축위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.2

Brucellosis, a bacterial zoonoses caused by the genus Brucella is responsible for abortion and infertility in cow. Brucellosis is causing economic loss in dairy industries and prevalent worldwide including Bangladesh but limited studies are devoted to determine the prevalence and its association with reproductive factors of dairy cows in Bangladesh. Therefore, the present study was conducted to determine the seroprevalence of brucellosis in dairy cattle using screening test Rose Bengal test (RBT) and the positive sera were further confirmed by indirect- ELISA. For this purpose, a total of 400 serum samples from dairy cows with history of abortion and various reproductive disorders were collected from the Kurigram district of Bangladesh for the detection of Brucella antibody. The overall prevalence of brucellosis in dairy cattle was 2.25%. Brucellosis in cases of abortion and repeat breeding was 8.3% and 2.8%, respectively. The results shows higher prevalence of brucellosis in cases of abortion followed by repeat breeding, while there was no seropositive cases from other reproductive disorders. Age-wise seroprevalence was found 3.0% in 2∼3 years age group and 2.0% in 4∼8 years age group. The prevalence of brucellosis in indigenous and cross-bred cattle was 3.6% and 1.7%, respectively. All the animals detected positive to brucellosis by RBT were not found to be positive by i-ELISA. However, the RBT might be a suitable screening test for the diagnosis of Brucella infection in field condition in Bangladesh. These data will help to develop effective disease prevention strategies.

Seroprevalence of brucellosis in small ruminants in selected area of Bangladash

Uddin, Mohammad Jasim,Rahman, Md Siddiqur,Akter, Sayeda Hasina,Hossain, Mohammad Arif,Islam, Md Taohidul,Islam, Md Ariful,Park, Jin-Ho,Song, Hee-Jong The Korean Society of Veterinary Service 2007 韓國家畜衛生學會誌 Vol.30 No.4

A seroprevalence study of small ruminant brucellosis was conducted in sheep and goat rearing selected areas of Mymensingh district and Dhaka district, Bangladesh, from March, 2005 to May, 2006. Sera from 62 sheep and 300 goats were tested by rose bengal plate test (RBPT), plate agglutination test (PAT), tube agglutination test (TAT) and mercaptoethanol test (MET). Out of the 62 sera tested 3.25% (n = 2) were positive to RBT, PAT and TAT and 4.84% (n = 3) were positive MET. In case of 300 goats, 1.67% (n = 5) were positive to RBT and PAT, 2% (n = 6) were positive to TAT and 2.33% (n = 7) were positive to MET. This investigation is the first of its type to be performed in small ruminants in Bangladesh. Higher prevalence rate (8.0 %) was found in BAU nutrition farm in case of sheep and 10 % in Bangladesh Agricultural University (BAU) Veterinary Clinic in case of goat while lower prevalence (0.0 %) was recorded in Pharmacology project and BAU adjacent villages in case of sheep and (0.0 %) in Dhamrai upazila in case of goats respectively. Brucella antibodies were more prevalent in sheep (8.84 %) than in goat (2.33 %).

Seroprevalence of brucellosis in small ruminants in selected area of Bangladash

( Mohammad Jasim Uddin ),( Md Siddiqur Rahman ),( Sayeda Hasina Akter ),( Mohammad Arif Hossain ),( Md Taohidul Islam ),( Md Ariful Islam ),( Jin Ho Park ),( Hee Jong Song ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.4

A seroprevalence study of small ruminant brucellosis was conducted in sheep and goat rearing selected areas of Mymensingh district and Dhaka district, Bangladesh, from March, 2005 to May, 2006. Sera from 62 sheep and 300 goats were tested by rose bengal plate test(RBPT), plate agglutination test(PAT), tube agglutination test(TAT) and mercaptoethanol test(MET). Out of the 62 sera tested 3.25%(n=2) were positive to RBT, PAT and TAT and 4.84%(n=3) were positive MET. In case of 300 goats, 1.67%(n=5) were positive to RBT and PAT, 2%(n=6) were positive to TAT and 2.33%(n=7) were positive to MET. This investigation is the first of its type to be performed in small ruminants in Bangladesh. Higher prevalence rate(8.0%) was found in BAU nutrition farm in case of sheep and 10% in Bangladesh Agri-cultural University(BAU) Veterinary Clinic in case of goat while lower prevalence (0.0%) was recorded in Pharmacology project and BAU adjacent villages in case of sheep and(0.0%) in Dhamrai upazila in case of goats respectively. Brucella antibodies were more prevalent in sheep (8.84%) than in goat (2.33%).