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Matsuda, Kiku,Chaudhari, Atul A.,Lee, John Hwa Elsevier 2010 Research in veterinary science Vol.89 No.2
<P><B>Abstract</B></P><P>An intestinal pathogenic <I>Escherichia coli</I> isolate from calf diarrhea, containing the <I>iutA</I>, <I>f17A</I>, <I>afa</I>-<I>8D</I>, and <I>cnf2</I> genes, was able to cause avian colibacillosis after experimental infection in chickens. Intra-tracheal inoculation and spray of this strain caused 10% of mortality and gross lesions, including airsacculitis, pericarditis, and perihepatitis. These results suggest that some bovine pathogenic <I>E. coli</I> can cause extra-intestinal infections in other animal species.</P>
Matsuda, Kiku,Chaudhari, Atul A.,Kim, Sam Woong,Lee, Kyeong Min,Lee, John Hwa EDP Sciences 2010 VETERINARY RESEARCH Vol.41 No.5
<P>To construct a novel live vaccine candidate for fowl typhoid (FT) caused by <I>Salmonella</I> Gallinarum (SG), the <I>lon</I> and <I>cpxR</I> genes that are related to host-pathogen interaction were deleted from a wild type SG using the allelic exchange method. The mutants were grown normally, as was the wild type. The biochemical properties of the mutants remained very similar to those of the wild-type, while JOL914 (Δ<I>lon</I>) and JOL916 (Δ<I>lon</I>Δ<I>cpxR</I>) were mucoid. Extracellular polysaccharide increased 30.6-, 1.3-, and 46.2-fold in JOL914, JOL915 (Δ<I>cpxR</I>), and JOL916, respectively. Dot-blot analysis demonstrated significant increases of FimA expression at 6.77-, 2.33-, and 3.90-fold for JOL914, JOL915, and JOL916, respectively. Internalizations of JOL914, JOL915, and JOL916, in chicken abdominal macrophages, were increased at 4.65-, 0.50-, and 2.72-fold, respectively. Virulences of JOL914, JOL915 and JOL916, analyzed by LD<SUB>50</SUB> using 1-week-old chickens, were attenuated approximately at 10<SUP>1</SUP>-, 10<SUP>1</SUP>-, and > 10<SUP>3</SUP>-fold, respectively. The oral inoculations of 2 × 10<SUP>7</SUP> cfu of the wild type, JOL914, JOL915 and JOL916 caused 55.6, 16.7, 22.2, and 0.0% mortality, respectively. Significantly moderate gross lesions of the liver and spleen were observed in the JOL916 group compared to the other groups. An induced immune response and significant peripheral mononuclear proliferation reaction were observed in the JOL916 group. At the protection against the wild type challenge, JOL916 offered 100% protection. Thus, the results of this study suggest that JOL916 among the mutants studied represented the safest and most effective live vaccine candidate against FT.</P>
임신 개에서의 Brucella abortus Strain RB51 접종시험
허진,박용호,Kiku, Matsuda,백병걸 한국수의공중보건학회 2001 예방수의학회지 Vol.25 No.3
Brucella abortus strain RB51 (SRB51) is an approved brucellosis vaccine in cattle. This study was caried out to know the immunological responses and abortion status in dogs inoculated with SRB51. Six pregnant dogs were subcutaneously inoculated with 1.0×109 CFU of SRB51. The animals were monitored at regular intervals by the standard tube agglutination test (STAT), Rose Bengal test (RBT), 2-mercaptoethanol rapid slide agglutination test (2ME-RSAT) and dot-blot. Using the whole cell antigen of SRB51, the mean antibody titer against SRB51 was below 1:10 at prior to inoculation in dot-blot. The titer gradually increased up to 1:370 by the 3rd week post-inoculation. In STAT and RBT, using the whole cell antigen of B. abortus 1119-3, and 2ME-RSAT, using the whole cell antigen of B. canis M-, the sera against SRB51 did not react with both antigens. SRB51 was not isolated from the blood of dogs subcutaneously inoculated with the bacteria. In addition, subcutaneous inoculation of SRB51 did not lead to abortion in pregnant dogs.
Infection of Brucella abortus Biotype 1 to Pregnant Sprague-Dawley Rat
Baek, Byeong Kirl,Siddiqur, Rahman Mohammad,Kiku, Mastuda,Lee, JooYoung,Hur, Jin,Lim, Chai Woong,Ibulaimu, Kakoma 한국수의공중보건학회 2001 예방수의학회지 Vol.25 No.4
한우에서 분리한 Brucella abortus biotype 1의 1×10^9cfu 균수를 임신 경험이 있는 암쥐와 임신 경험이 없는 처녀 쥐에게 임신 7일, 14일 그리고 18일째 각각 경피 감염시켰다. 인공 감염된 군은 간, 비장 조직에서 AMOS PCR과 조직의 세균 배양 시험으로 균체를 확인하였다. 감염 쥐의 체온은 최고 38℃까지 상승하였으나 대조군에서는 상승하지 않았다. 감염시킨 군에서는 사산, 유산, 조산, 체온의 재 상승 등이 없었으나 비장확대, 자궁염, 림프절의 종대, 태반염과 염증 증상이 육안적으로 관찰되었다. 본 연구를 통하여 Brucella 연구의 모델로서 쥐의 사용 가능성과 브루셀라 보균체로서의 역할 가능성을 있음을 알 수 있었다.
급식패턴에 따른 성장기 개의 신체지수(BCS)에 대한 조사 및 고찰
조성진,마쓰다 기꾸,이성호,박영석,김병수 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10
Feeding Puppy at the first 6 months is substantially important and give effects on general health status during whole life of the animal. Concerning to feeding method, any one of free choice feeding, time limiting feeding, amount limiting feeding is usually selected by owners. We surveyed recent 3 years' 1251 veterinary medical records in a local private veterinary hospital and selected all cases (661) that visited the hospital from 0 to 6 months of age. The followings were investigated: sex, breed, present feeding style, whether Body Condition Score (BCS) was low or not, whether client education on feeding style was done or not. The results showed: (1) small breeds that occupying 66.3% were most often fed time-and-amount limited, and (2) the restricted feeding was significantly related to low BCS. Owner pre-education about feeding should be encouraged especially on small breeds.
Chaudhari, Atul A,Matsuda, Kiku,Lee, John Hwa American Association of Avian Pathologists [etc.] 2013 Avian diseases Vol.57 No.1
<P>An attenuated Salmonella (deltalon, deltacpxR, and deltaasdA16) delivery system containing the genes encoding P-fimbriae (papa and papG), aerobactin receptor (iutA), and CS31A surface antigen (clpG) of avian pathogenic Escherichia coli (APEC) was constructed, and its potential as a vaccine candidate against APEC infection in chickens was evaluated. The birds were divided into three groups designated group A (nonvaccinated control), group B (given a single immunization), and group C (administered prime and boost immunizations). Prime and booster vaccinations with the constructions were administered to 1-day-old and 14-day-old birds, respectively. Immune responses were measured postimmunization, and the birds were challenged via an intra-air sac route with a virulent APEC strain at the second, third, and fourth weeks of age. Group B birds were partially protected against the challenge and showed increased levels of plasma immunoglobulin (Ig)G, mucosal IgA antibodies, and lymphocyte proliferation. Group C birds showed greater protection against the challenge, with significantly stronger immune responses compared with the birds in the other groups. Overall, our data suggest that the Salmonella delivery system with recombinant constructs is capable of inducing robust immune responses and induces effective protection against colibacillosis caused by APEC.</P>