Role of Nucleation-Promoting Factors in Mouse Early Embryo Development

Wang, Qiao-Chu,Liu, Jun,Wang, Fei,Duan, Xing,Dai, Xiao-Xin,Wang, Teng,Liu, Hong-Lin,Cui, Xiang-Shun,Sun, Shao-Chen,Kim, Nam-Hyung Cambridge University Press 2013 Microscopy and microanalysis Vol.19 No.3

<B>Abstract</B><P>During mitosis nucleation-promoting factors (NPFs) bind to the Arp2/3 complex and activate actin assembly. JMY and WAVE2 are two critical members of the NPFs. Previous studies have demonstrated that NPFs promote multiple processes such as cell migration and cytokinesis. However, the role of NPFs in development of mammalian embryos is still unknown. Results of the present study show that the NPFs JMY and WAVE2 are critical for cytokinesis during development of mouse embryos. Both JMY and WAVE2 are expressed in mouse embryos. After injection of JMY or WAVE2 siRNA, all embryos failed to develop to the morula or blastocyst stages. Moreover, using fluorescence intensity analysis, we found that the expression of actin decreased, and multiple nuclei were observed within a single cell indicating that NPFs-induced actin reduction caused the failure of cell division. In addition, injection of JMY and WAVE2 siRNA also caused ARP2 degradation, indicating that involvement of NPFs in development of mouse embryos is mainly through regulation of ARP2/3-induced actin assembly. Taken together, these data suggested that WAVE2 and JMY are involved in development of mouse embryos, and their regulation may be through a NPFs-Arp2/3-actin pathway.</P>

Apoptosis induced in vivo by new type gosling viral enteritis virus

Shun Chen,Anchun Cheng,Mingshu Wang,Dekang Zhu,Renyong Jia,Qihui Luo,Hengmin Cui,Yi Zhou,Yin Wang,Zhiwen Xu,Zhengli Chen,Xiaoyue Chen,Xiaoyu Wang 대한수의학회 2011 JOURNAL OF VETERINARY SCIENCE Vol.12 No.4

In this study, apoptosis was induced by new type gosling viral enteritis virus (NGVEV) in experimentally infected goslings is reported in detail for the first time. After 3-day-old goslings were orally inoculated with a NGVEV-CN strain suspension, the time course of NGVEV effects on apoptotic morphological changes of the internal tissues was evaluated. These changes were observed by histological analysis with light microscopy and ultrastructural analysis with transmission electron microscopy. DNA fragmentation was assessed with a terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay and DNA ladder analysis. A series of characteristic apoptotic morphological changes including chromatin condensation and margination, cytoplasmic shrinkage, plasma membrane blebbing, and formation of apoptotic bodies were noted. Apoptosis was readily observed in the lymphoid and gastrointestinal organs, and sporadically occurred in other organs after 3 days post-infection (PI). The presence and quantity of TUNEL-positive cells increased with infection time until 9 days PI. DNA extracted from the NGVEV-infected gosling cells displayed characteristic 180~200 bp ladders. Apoptotic cells were ubiquitously distributed, especially among lymphocytes, macrophages, monocytes, and epithelial and intestinal cells. Necrosis was subsequently detected during the late NGVEV-infection phase, which was characterized by cell swelling, plasma membrane collapse, and rapidly lysis. Our results suggested that apoptosis may play an important role in the pathogenesis of NGVE disease.

A DFT study of the geometrical structures and electronic properties of CumMn (m + n = 6, M = Fe, Co, Ni) clusters

Wang Su Juan,Zhou Dan,Hu Yan Fei,Tang Cui Ming,Wang Qiao Zhi,Chen Shun Ling 한국물리학회 2021 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.79 No.3

In this work, we investigated bimetallic CumMn (m + n = 6, M = Fe, Co, Ni) clusters on the basis of the density functional theory at the M06L level. While changing the structure and composition, our team studied the geometrical structures, relative stabilities and electronic magnetic moments of these clusters. We found that the average atomic binding energies vary linearly with the number of doping atoms in the CumFen and the CumNin clusters. The values of electron affinity (EA) and the ionization potential (IP) for the CumNin clusters are closer to those of pure Cu clusters compared with the others. A negative value of the EA is only seen in CumFen clusters. The electronic magnetic moments of these clusters indicate that most of them exhibit a local minimum when doped with three atoms. Moreover, we further analyzed the HOMO–LUMO gaps, the natural population, and the geometrical structures.

Barriers and Problems: How to Develop University Sport in China

( Wang Yong Shun ) 한국체육학회 2015 국제스포츠과학 학술대회 Vol.2015 No.1

Purpose: Some experiences and lessons has been obtained since 1986,when china begin to develop university sports and hold the related games. While there are still some problems need to solve in the development of university sports in China. In the new condition of education and spot system’s reform, we have to answer these questions, how to develop university sport, how to understand the role of university sport what is the purpose of university sport, what are the main factors in the development of college sport. Method: literature review, expert interview and questionnaire survey. Conclusion: first, The important role of university sport is helping improve the development of university, attracting more people participate in sport, constructing the college culture and bringing economical benefits to university. Second, due to the sport management system’s reform, there is a good environment for the development of university sport in china. Third, the inside environment is vital to china’s university sport, the management framwork is so symple and lack of management and laws. Forth, there are some problems in athletes students, they always have problems in study and job finding, so there are should be some changes in recruiting and cultivating these students.

GROUND OBSERVATIONS OF SPRITES AND OTHER TLES IN TAIWAN

WANG YUN-CHING,HSU RUE-RON,SU HAN-TZONG,CHEN ALFRED BING-CHIH,LEE YI-JEN,KUO CHENG-LING,TSAY WEAN-SHUN,CHANG CHAN-KAO,WANG SHI-CHUN,LEE LOU-CHUANG,LIU TIE-YUE The Korean Astronomical Society 2005 Journal of The Korean Astronomical Society Vol.38 No.2

Sprites, elves and blue jets are collectively denominated as the upper atmospheric transient luminous events (TLEs). They are recently discovered optical flashes between active thunderstorms and the ionosphere. In this report, a brief introduction to the most important characteristics of TLEs is given. Since 2001, scientists from the National Cheng Kung University have been performing yearly summer campaigns from various locations in Taiwan. The main achievements of their yearly campaign are presented.

Adaptive State-of-Charge Estimation Method for an Aeronautical Lithium-ion Battery Pack Based on a Reduced Particle-unscented Kalman Filter

Shun-Li Wang,Chun-Mei Yu,Carlos Fernandez,Ming-Jie Chen,Gui-Lin Li,Xiao-Han Liu 전력전자학회 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.4

A reduced particle-unscented Kalman filter estimation method, along with a splice-equivalent circuit model, is proposed for the state-of-charge estimation of an aeronautical lithium-ion battery pack. The linearization treatment is not required in this method and only a few sigma data points are used, which reduce the computational requirement of state-of-charge estimation. This method also improves the estimation covariance properties by introducing the equilibrium parameter state of balance for the aeronautical lithium-ion battery pack. In addition, the estimation performance is validated by the experimental results. The proposed state-of-charge estimation method exhibits a root-mean-square error value of 1.42% and a mean error value of 4.96%. This method is insensitive to the parameter variation of the splice-equivalent circuit model, and thus, it plays an important role in the popularization and application of the aeronautical lithium-ion battery pack.

Development and Design of Ice and Snow Landscape System Based on Network and Information Technology

Wang Shun-hui,Lin Xue-wei 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.1

Ice and Snow Art Landscape is unique urban landscape form in cold region. It is also artistic form reflecting the regional characteristics and cultural connotations as the symbol of the city. It has the important meaning for urban tourism industry and related industries. The construction of Urban Ice and Snow Art Landscape Information Database is an important channel promoting the development of Urban Ice and Snow Art. The research is based on modern network information technology, taking Urban Ice and Snow Art Landscape as research object. It puts forward on idea constructing Urban Ice and Snow Art Landscape Information Database, and design system architecture in the background using the technology of database, front design using DW, including Data Dictionary, E-R model, SQL, PHP and WEB publish. The development and Application of Urban Ice and Snow Art Landscape Information Database, is very necessary for the great-leap-forward development of Urban Ice and Snow Art Landscape.

Teaching Switching Converter Design Using Problem-Based Learning with Simulation of Characterization Modeling

Shun-Chung Wang,Yih-Chien Chen,Juing-Huei Su 전력전자학회 2010 JOURNAL OF POWER ELECTRONICS Vol.10 No.6

In this paper, teaching in a “switching converter (SC) design” course using problem-based learning (PBL) with dynamicbehavior-model simulation, given at Lunghwa University of Science and Technology (LHU), Taiwan, is proposed. The devised methodology encourages students to design and implement the SCs and regulate the controller’s parameters in frequency domain by using ‘sisitool’ (‘bode’) in the MATLAB toolbox. The environment of PBL with converter characterization modeling and simulation reforms the learning outcome greatly and speeds up the teaching-learning process. To qualify and evaluate the learning achievements, a hands-on project cooperated with the continuous assessment approach is performed to modulate the teaching pace and learning direction in good time. Results from surveys conducted in the end of the course provided valuable opinions and suggestions for assessing and improving the learning effect of the proposed course successively. Positive feedbacks from the examinations, homework, questionnaires, and the answers to the lecturer’s quizzes during class indicated that the presented pedagogy supplied more helpfulness to students in comparisons with conventional teaching paradigm, their learning accomplishments were better than expected as well.

Teaching Switching Converter Design Using Problem-Based Learning with Simulation of Characterization Modeling

Wang, Shun-Chung,Chen, Yih-Chien,Su, Juing-Huei The Korean Institute of Power Electronics 2010 JOURNAL OF POWER ELECTRONICS Vol.10 No.6

In this paper, teaching in a "switching converter (SC) design" course using problem-based learning (PBL) with dynamicbehavior- model simulation, given at Lunghwa University of Science and Technology (LHU), Taiwan, is proposed. The devised methodology encourages students to design and implement the SCs and regulate the controller's parameters in frequency domain by using 'sisitool' ('bode') in the MATLAB toolbox. The environment of PBL with converter characterization modeling and simulation reforms the learning outcome greatly and speeds up the teaching-learning process. To qualify and evaluate the learning achievements, a hands-on project cooperated with the continuous assessment approach is performed to modulate the teaching pace and learning direction in good time. Results from surveys conducted in the end of the course provided valuable opinions and suggestions for assessing and improving the learning effect of the proposed course successively. Positive feedbacks from the examinations, homework, questionnaires, and the answers to the lecturer's quizzes during class indicated that the presented pedagogy supplied more helpfulness to students in comparisons with conventional teaching paradigm, their learning accomplishments were better than expected as well.

Design and Implementation of Online Mobile Game based on J2ME

WANG Shun-hui,ZOU Jie 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.7

Online mobile game is a hot industry. It is a comprehensive industry including program design, planning and art design. This paper analyzed JAVA program and mainstream game development environment, then developed a mobile game based on J2ME, including modeling design,architecture design, class.