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KIM, SHUKHO,LIM, OK YOUNG,KIM, SEONG HAN,KIM, JUN YOUNG,KANG, YEON HO,LEE, BOK KWON 한국미생물 · 생명공학회 2003 Journal of microbiology and biotechnology Vol.13 No.1
In early 2002, over 200 people in the city of Pusan. Korea suffered from paratyphoid fever resulting from Salmonella Paratyphi A infection. Antimicrobial susceptibility tests and Xbal pulsed-field gel electrophoresis (PEGE) were conducted to 54 Salmonella Paratyphi A isolated from humans during the period of 1998 to 2002. Most of the isolated (83%) were only nalidixic acid-resistant and 78% were X 1 PEGE patterns. Also, we measured the MIC of ciprofloxacin and screened gyrA mutation(s) using allele-specific PCR and restriction fragment length polymorphism (AS-PCR-RFLP). The representative 5 isolates in 2002 and 1 isolate in 2000 were 1 ㎍/ml of MIC and had mutation at the 83rd codon in gyrA. These data suggest that the outbreak in the early 2002 might have been due to dissemination of the strain present in 2000. Also, decreased susceptibility to ciprofloxacin was partly due to the mutation at the 83rd codon in gyrA.
실시간 공중보건 감시와 식품매개질환 집단발생 대처를 위한 국가 전염병 조기경보 시스템 구축 - PulseNet Korea
김석호,이상원,김성한,김준영,이혜영,강연호,박미선,이복권 대한감염학회 2006 감염과 화학요법 Vol.38 No.6
Foodborne illnesses and big outbreaks have been increased because of the widespread of lunch distribution at school, mass production of food products, and international food trades. It is important to find the origin of contamination by various pathogens in an early stage of the outbreaks for the disease control and prevention. For the purpose of construction of the early warning system, Korea National Institute of Health (KNIH) inaugurated PulseNet Korea in 2005. The organization of PulseNet Korea consists of KNIH as a center and the participating laboratories including Korea Food & Drug Administration (KFDA), National Veterinary Research & Quarantine Station (NVRQS), and regional Institutes of Health & Environment. PulseNet Korea has focused on training researchers from participating laboratories as well as playing an important role in PulseNet International. In this review, PulseNet Korea construction is introduced as a national early warning system for timely surveillance of foodborne diseases.
A Simple Colorimetric Method for Testing Antimicrobial Susceptibility of Biofilmed Bacteria
Shukho Kim,김미진,Hee Young Kang,설성용,조동택,김정민 한국미생물학회 2010 The journal of microbiology Vol.48 No.5
This study introduces a simple colorimetric method which can measure the antimicrobial susceptibility of bacteria in biofilms using trimethyl tetrazolium chloride (TTC) as an indicator of viable bacteria. The new method was utilized for the evaluation of antibiotic susceptibility of Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus biofilms.
Kim, Shukho,Rahman, Marzia,Seol, Sung Yong,Yoon, Sang Sun,Kim, Jungmin American Society for Microbiology 2012 Applied and environmental microbiology Vol.78 No.17
<B>ABSTRACT</B><P>We isolated a new lyticPseudomonas aeruginosaphage that requires type IV pili for infection. PA1Ø has a broad bactericidal spectrum, covering Gram-positive and Gram-negative bacteria, and can eradicate biofilm cells. PA1Ø may be developed as a therapeutic agent for biofilm-related mixed infections withP. aeruginosaandStaphylococcus aureus.</P>
Kim, Shukho,Chun, Sung-Guen,Lim, Ok-Young,Park, Mi-Sun,Kang, Yeon-Ho,Park, Yong-Ho,Lee, Bok-Kwon The Microbiological Society of Korea 2004 The journal of microbiology Vol.42 No.1
Salmonella enterica serovar Typhimurium DT104 (Salmonella Typhimurium DT104 or DT104) has been emerging as a common pathogen for human in Korea since 1997. In order to compare the genomic relationship and to search for the dominant strains in Korea, we conducted pulsed-field gel electrophoresis (PFGE) and IS200 fingerprinting of 25 epidemiological unrelated isolates from human and animals from Korea and cattle from America. Two Salmonella Typhimurium DT104 isolates from human in Korea and all 8 isolates from American cattle had indistinguishable patterns from the PFGE and IS200 fingerprinting but multidrug-resistant Salmonella Typhimurium isolates, including DT104, from Korean animals had diverse genetic patterns. The data suggest that a dominant DT104 strain might have circulated between Korean and American cattle and that it had a high level of clonality.
Shukho Kim,Sung Guen Chun,Ok Young Lim,Mi Sun Park,Yeon Ho Kang,박용호,이복권 한국미생물학회 2004 The journal of microbiology Vol.42 No.1
Salmonella enterica serovar Typhimurium DT104 (Salmonella Typhimurium DT104 or DT104) has been emerging as a common pathogen for human in Korea since 1997. In order to compare the genomic relationship and to search for the dominant strains in Korea, we conducted pulsed-field gel electrophoresis (PFGE) and IS200 fingerprinting of 25 epidemiological unrelated isolates from human and animals from Korea and cattle from America. Two Salmonella Typhimurium DT104 isolates from human in Korea and all 8 isolates from American cattle had indistinguishable patterns from the PFGE and IS200 fingerprinting but multidrug-resistant Salmonella Typhimurium isolates, including DT104, from Korean animals had diverse genetic patterns. The data suggest that a dominant DT104 strain might have circulated between Korean and American cattle and that it had a high level of clonality.