수중 시체에서 내부 장기 및 대조수에 대한 규조류의 분석

조갑래,서원준,함석훈,이종석,최동호,전병원,김양정,김광훈 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

THEORETICAL AND EXPERIMENTAL STUDY ON FLOW CHARACTERISTICS OF A DIAPHRAGM PUMP FOR UREA-SCR SYSTEMS

Shu Dong Yang,You Cheng Shi,Xi Wei Pan,Yin Shui Liu 한국자동차공학회 2019 International journal of automotive technology Vol.20 No.4

Urea selective catalytic reduction (SCR) is the primary technology used to reduce the nitrogen oxides (NOx) of diesel engine exhaust. To meet the requirements of an SCR system, a novel type of miniature diaphragm pump was designed. Based on the theory of large deflection of annular plates, the equilibrium equations of a diaphragm with a rigid inclusion were established, and the equations were solved by the nondimensional method and the finite difference method. Theoretical and approximated flow model for this pump were proposed. A theoretical relationship between back pressure, rigid inclusion size and volumetric efficiencies were calculated. To verify the validity of theoretical model, a prototype pump was fabricated and tested. Experimental results demonstrated that the flow is proportional to the pump speed. The deviation between theoretical, approximated flow and experimental flow was less than 4 % and 9.4 %, respectively. The difference between theoretical and experimental volumetric efficiency was varied from 2.7 % to 6.1 % when back pressure changed from 0 to 0.9 MPa. The volumetric efficiency was growing with the increasing of the rigid inclusion size. The pressures in the working chamber showed almost the same overall trends between the theoretical results and experimental values. The experimental results show that the proposed theoretical model is effective.

Increased Serotonin Signaling Contributes to the Warburg Effect in Pancreatic Tumor Cells Under Metabolic Stress and Promotes Growth of Pancreatic Tumors in Mice

Jiang, Shu-Heng,Li, Jun,Dong, Fang-Yuan,Yang, Jian-Yu,Liu, De-Jun,Yang, Xiao-Mei,Wang, Ya-Hui,Yang, Min-Wei,Fu, Xue-Liang,Zhang, Xiao-Xin,Li, Qing,Pang, Xiu-Feng,Huo, Yan-Miao,Li, Jiao,Zhang, Jun-Feng Elsevier 2017 Gastroenterology Vol.153 No.1

<P><B>Background & Aims</B></P> <P>Desmoplasia and poor vascularity cause severe metabolic stress in pancreatic ductal adenocarcinomas (PDACs). Serotonin (5-HT) is a neuromodulator with neurotransmitter and neuroendocrine functions that contributes to tumorigenesis. We investigated the role of 5-HT signaling in the growth of pancreatic tumors.</P> <P><B>Methods</B></P> <P>We measured the levels of proteins that regulate 5-HT synthesis, packaging, and degradation in pancreata from Kras<SUP>G12D/+</SUP>/Trp53<SUP>R172H/+</SUP>/Pdx1-Cre (KPC) mice, which develop pancreatic tumors, as well as in PDAC cell lines and a tissue microarray containing 81 human PDAC samples. We also analyzed expression levels of proteins involved in 5-HT synthesis and degradation by immunohistochemical analysis of a tissue microarray containing 311 PDAC specimens, and associated expression levels with patient survival times. 5-HT level in 14 matched PDAC tumor and non-tumor tissues were analyzed by ELISA. PDAC cell lines were incubated with 5-HT and cell survival and apoptosis were measured. We analyzed expression of the 5-HT receptor HTR2B in PDAC cells and effects of receptor agonists and antagonists, as well as HTR2B knockdown with small hairpin RNAs. We determined the effects of 5-HT stimulation on gene expression profiles of BxPC-3 cells. Regulation of glycolysis by 5-HT signaling via HTR2B was assessed by immunofluorescence and immunoprecipitation analyses, as well as by determination of the extracellular acid ratio, glucose consumption, and lactate production. Primary PDACs, with or without exposure to SB204741 (a selective antagonist of HTR2B), were grown as xenograft tumors in mice, and SB204741 was administered to tumor-bearing KPC mice; tumor growth and metabolism were measured by imaging analyses.</P> <P><B>Results</B></P> <P>In immunohistochemical analysis of a tissue microarray of PDAC specimens, increased levels of TPH1 and decreased level of MAOA, which regulate 5-HT synthesis and degradation, correlated with stage and size of PDACs and shorter patient survival time. We found levels of 5-HT to be increased in human PDAC tissues compared with non-tumor pancreatic tissues, and PDAC cell lines compared with non-transformed pancreatic cells. Incubation of PDAC cell lines with 5-HT increased proliferation and prevented apoptosis. Agonists of HTR2B, but not other 5-HT receptors, promoted proliferation and prevented apoptosis of PDAC cells. Knockdown of HTR2B in PDAC cells, or incubation of cells with HTR2B inhibitors, reduced their growth as xenograft tumors in mice. We observed a correlation between 5-HT and glycolytic flux in PDAC cells; levels of metabolic enzymes involved in glycolysis, the phosphate pentose pathway, and hexosamine biosynthesis pathway increased significantly in PDAC cells following 5-HT stimulation. 5-HT stimulation led to formation of the HTR2B–LYN–p85 complex, which increased PI3K–Akt–mTOR signaling and the Warburg effect by increasing protein levels of MYC and HIF1A. Administration of SB204741 to KPC mice slowed growth and metabolism of established pancreatic tumors and prolonged survival of the mice.</P> <P><B>Conclusions</B></P> <P>Human PDACs have increased levels of 5-HT, and PDAC cells increase expression of its receptor, HTR2B. These increases allow for tumor glycolysis under metabolic stress and promote growth of pancreatic tumors and PDAC xenograft tumors in mice.</P>

Effects of edge crack on the vibration characteristics of delaminated beams

Yang Liu,Dong W. Shu 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.53 No.4

Delaminations and cracks are common failures in structures. They may significantly reduce thestiffness of the structure and affect their vibration characteristics. In the present study, an analytical solution is developed to study the effect of an edge crack on the vibration characteristics of delaminated beams. The rotational spring model, the „free mode‟ and „constrained mode‟ assumptions in delamination vibration areadopted. This is the first study on how an edge crack affects the vibration characteristic of delaminated beams and new nondimensional parameters are developed accordingly. The crack may occur inside or outside the delaminated area and both cases are studied. Results show that the effect of delamination length and thickness-wise location on reducing the natural frequencies is aggravated by an increasing crack depth. The location of the crack also influences the effect of delamination, but such influence is different between crack occurring inside and outside the delaminated area. The difference of natural frequencies between „free mode‟ and „constrained mode‟ increases then decreases as the crack moves from one side of the delaminated region to the other side, peaking at the middle. The analytical results of this study can serve as the benchmark for FEM and other numerical solutions.

MicroRNAs as Promising Biomarkers for Tumor-staging: Evaluation of MiR21 MiR155 MiR29a and MiR92a in Predicting Tumor Stage of Rectal Cancer

Yang, Yun,Peng, Wei,Tang, Tian,Xia, Lin,Wang, Xiao-Dong,Duan, Bao-Feng,Shu, Ye Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Objective: In this study, tumor-stage predictive abilities of miR21, miR155, miR29a and miR92a were evaluated in rectal cancer (RC). Methods: Expression of miR21, miR155, miR29a and miR92a was detected and quantitated in tumor tissue and in adjacent normal tissue from 40 patients by TaqMan MicroRNA assay. Results: Significant overexpression of miR21, miR155, miR29a and miR92a was observed in RC tissues. While high expression of miR21, miR155 and miR29a in N1-2 and C-D stages presented a potential correlation with N and Duke stages, partial correlation analysis suggested that only miR155 rather than miR21 and miR29a played a greater influencing role. Receiver operating characteristics (ROC) curve analysis showed that miR155 could discriminate N0 from N1-2 with 85.0% sensitivity and 85.0% specificity, N2 from N0-1 with 90.0% sensitivity and 96.7% specificity, and C-D stage from A-B stage with 81.0% sensitivity and 84.2% specificity. Conclusions: Increase in expression of miR155 might represent a novel predictor for RC N and Dukes staging.

Effects of edge crack on the vibration characteristics of delaminated beams

Liu, Yang,Shu, Dong W. Techno-Press 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.53 No.4

Delaminations and cracks are common failures in structures. They may significantly reduce the stiffness of the structure and affect their vibration characteristics. In the present study, an analytical solution is developed to study the effect of an edge crack on the vibration characteristics of delaminated beams. The rotational spring model, the 'free mode' and 'constrained mode' assumptions in delamination vibration are adopted. This is the first study on how an edge crack affects the vibration characteristic of delaminated beams and new nondimensional parameters are developed accordingly. The crack may occur inside or outside the delaminated area and both cases are studied. Results show that the effect of delamination length and thickness-wise location on reducing the natural frequencies is aggravated by an increasing crack depth. The location of the crack also influences the effect of delamination, but such influence is different between crack occurring inside and outside the delaminated area. The difference of natural frequencies between 'free mode' and 'constrained mode' increases then decreases as the crack moves from one side of the delaminated region to the other side, peaking at the middle. The analytical results of this study can serve as the benchmark for FEM and other numerical solutions.

성범죄 증거물에서 관계남성 유전자형 검출율 향상에 관한 연구

최동호(Dong-Ho Choi),김양정(Yang-Jung Kim),전병원(Byung-Won Chun),신상철(Sang-Churl Shin),김경숙(Kyung-Sook Kim),조갑래(Gap-Rae Jo),함석훈(Suk-Hoon Ham),이종석(Jong-Seok Lee),서원준(Won-Jun Shu),김광훈(Kwang-Hoon Kim) 한국법과학회 2004 한국법과학회 학술대회 Vol.2004 No.1

Glulam Beam-to-Column Connection with Coupled Steel Rods with Screwheads: Concept and Experimental Verifications

Zhan Shu,Bo Ning,Hanlin Dong,Zheng Li,Zengxin Peng,Yiyu Yang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.6

The bolted connections with slotted-in steel plates are basic beam-to-column connections of modern post-and-beam timber building structures. However, due to significantly pinched rotational behaviors, the moment resistance of such connections are usually ignored in design. In this study, the connection-level moment resistance is enabled for modern glulam frame structures with an innovative connection design. A pair of long steel rods with screwheads is designed to generate a force couple at the sides of the connection. The proposed connection could be considered as the key lateral force resisting component, indicating a significant reduction of lateral braces and shear walls. Specimens of the target connection were carefully designed and tested in the lab to obtain their mechanical properties and failure modes. Finite element models were established and calibrated according to the tested data. The moment-rotation curves of the new connection could be easily estimated through a fitted formula. Moreover, the suggestions to improve the proposed connection is also discussed based on the observed damage modes. Results show that the new connection could be treated as rigid moment-resisting connections while the ductility could be further improved.

李白生卒年硏究中的問題分析

楊?生(Yang Xu-Sheng),沈曙東(Shen Shu-Dong) 동아인문학회 2010 동아인문학 Vol.17 No.-

한 작기의 생몰년에 대한 연구는 틀림없이 작기에 대한 연구에서 중요한 내용이다. 특히 고대작가에 대한 연구는 더욱 그러하다. 그렇기 때문에 이백이 생몰년 문제는 그 시대 이백에 대한 연구에서 가장 주목을 받는 문제로 되어 많은 “새로운 주장”들이 나오게 되었다. 두말할 나위 없이 이런 주제에 문장을 더 뛰어나게 하려고 공을 들이는 “백가쟁명”은 이백의 생몰년에 대한 심도 깊은 연구에 유익한 점을 가져다 줄 것임은 이다. 그러나 만약 한 문제에 대한 연구가 특히 생몰년과 같은 유일한 결론이 나는 문제일 경우에는 일치된 결론을 얻을 수 없는 경지에 빠지게 되는데 이것은 학술연구자들이 바라는 바는 아니다. 그리하여 이백의 생몰년에 대한 결론이 사실에 좀 더 부합되게 하기 위해서는 “새로운 주장”에 나타나는 문제에 대하여 다시 한 번 정리해볼 필요가 있다고 생각된다. 생몰년에 대한 확정은 작가(역사인물) 연구에 있어서 중요한 일이다. 하물며 이백의 생몰년은 더욱 그러하다. 우리는 이백의 생몰년 연구에서 나타나는 문제에 대하여 순서대로 정리를 하였다. 실제로 이백의 생몰년의 연구와 확정에 도움이 될 것으로 사료된다.

DNA Repair Gene ERCC1 and XPD Polymorphisms Predict Glioma Susceptibility and Prognosis

Chen, Da-Qing,Yao, Dong-Xiao,Zhao, Hong-Yang,Yang, Shu-Juan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Aims: We conducted a case-control study in a Chinese population to clarify the association between polymorphisms in ERCC1 and XPD and susceptibility and survival of glioma. Methods: A total of 393 cases and 410 controls were selected from March 2007 to December 2011. Genotyping of ERCC1 and XPD was conducted by TaqMan assays using the ABI Prism 7911HT Sequence Detection System. All analyses were performed using the STATA statistical package. Results: Polymorphisms in ERCC1 118C/T, ERCC1 8092C/A and XPD Asp312Asn showed no statistically significant difference between glioma cases and controls. However, individuals with the XPD 751Gln/Gln genotype had an increased risk of developing glioma compared with those with the Lys/Lys genotype (adjusted OR=1.64, 95% CI: 1.06-2.89). The ERCC1 118T/T genotype was associated with significantly higher median survival than the ERCC1 C/C genotype (HR=0.67, 95%CI=0.35-0.96). In addition, individuals with XPD 751Gln/Gln had a lower median survival time than XPD Lys/Lys carriers (HR=0.54, 95%CI=0.37-0.93). Conclusion: In conclusion, we observed that the XPD 751Gln/Gln genotype is associated with glioma susceptibility, and ERCC1 118 T/T and XPD 751Gln/Gln genotypes confer a significantly better prognosis.