Molecular Cloning, Segmental Distribution and Ontogenetic Regulation of Cationic Amino Acid Transporter 2 in Pigs

Zou, Shi-geng,Zhi, Ai-min,Zhou, Xiang-yan,Zuo, Jian-jun,Zhang, Yan,Huang, Zhi-yi,Xu, Ping-Wen,Feng, Ding-yuan Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.5

The goal of this study was to elucidate the expression and segmental distribution of the glomerular cationic amino acid metabolism transporter-2 (CAT-2) and thus to improve our understanding of porcine cationic amino acid transporters and amino acid absorption. Porcine CAT-2 was cloned, sequenced and characterized. The predicted amino acid sequence of porcine CAT-2 shared 86.1% and 92.1% identity with human and mouse CAT-2A, respectively. The tissue distribution patterns and ontogenic changes of CAT-2 mRNAs were determined by real-time Q-PCR. The results showed that porcine CAT-2 was highly expressed in the heart and intestinal tract (duodenum, ileum and jejunum). In addition, the mRNA of CAT-2 was found in liver, lung, kidney, brain and muscle. Within the intestinal tract, CAT-2 mRNA was most abundant in the ileum and rarely expressed in the duodenum. In the duodenum, the levels of CAT-2 mRNA reached their peak on day 7 (p<0.05) while in the jejunum, levels were low on day 1 and 7 and increased rapidly after day 26 before peaking on days 30 and 60 (p<0.05). The levels then dramatically decreased by day 90 (p<0.05). In the ileum, levels achieved their maximum on day 30 and then decreased significantly on day 60 (p<0.05).

Cloning and Spatiotemporal Expression Analysis of Bombyx mori elav, an Embryonic Lethal Abnormal Visual Gene

Geng Xian Wang,Ying Liu,Yang Hu Sima,Sheng Xiang Zhang,Shi Qing Xu 한국잠사학회 2009 International Journal of Industrial Entomology Vol.18 No.2

Embryonic lethal abnormal visual (elav) is a lethal gene in Drosophila inducing the abnormal development and function of nervous system. We cloned a Bmelav gene by bioinformatics and biological experiment, based on sequence of ELAV protein and dbEST of Bombyx mori. The full-length of Bm-elav cDNA is 1498 bp, contains a 906 bp open read frame (ORF) encoding a precursor of 301 amino acid residues with a calculated molecular weight of 34 kDa and pI of 8.99. Bm-ELAV protein precursor contains three RNA recognition motifs (RRM) in 24~91, 110~177 and 222~295 bit amino acid residues respectively, and belongs to RNA-binding protein family. Bm-ELAV shared varying positives, ranging from 56% to 60% (Identities from 41% to 45%), with RRM from other species of Xenopus tropicalis, Apis mellifera, Tribolium castaneum, Branchiostoma belcheri and Drosophila. Gene localization indicated that Bm-elav is a single-copy gene, gene mapping within 12-chromosome from 7916.68 knt to 7918.16 knt region of nscaf2993. Spatiotemporal expressions pattern analysis revealed that Bm-elav expressed higher in most tested tissues and developmental stages in whole generation, such as silk gland, fat body, midgut, hemopoietic organ and ovary, but almost no expression in terminated diapause eggs. This suggested that the expression of Bm-elav in early developmental embryonic stages might induce abnormal development like in Drosophila. Cloning of the Bmelav gene enables us to test its potential role in controlling pests by transferring the gene into field lepidopteran insects in the future.

A Hybrid Link Protection Scheme for Ensuring Network Service Availability in Link-state Routing Networks

Haijun Geng,Han Zhang,Xingang Shi,Zhiliang Wang,Xia Yin,Ju Zhang,Zhiguo Hu,Yong Wu 한국통신학회 2020 Journal of communications and networks Vol.22 No.1

The internet is playing an increasingly crucial role inboth personal and business activities. In addition, with the emergenceof real-time, delay sensitive and mission-critical applications,stringent network availability requirement is put forward for internetservice providers (ISPs). However, commonly deployed intradomainlink-state routing protocols react to link failures by globallyexchanging link state advertisements and recalculating routingtable, inevitably causing significant forwarding discontinuityafter a failure. Therefore, the loop-free criterion (LFC) approachhas been widely deployed by many ISPs for coping with the singlenetwork component failure scenario in large internet backbones. The success of LFC lies in its inherent simplicity, but this comesat the expense of letting certain failure scenarios go unprotected. To achieve full failure coverage with LFC without incurring significantextra overhead, we propose a novel link protection scheme,hybrid link protection (HLP), to achieve failure resilient routing. Compared to previous schemes, HLP ensures high network availabilityin a more efficient way. HLP is implemented in two stages. Stage one provides an efficient LFC based method (MNP-e). Thecomplexity of the algorithm is less than that of Dijkstra’s algorithmand can provide the similar network availability with LFC. Stage two provides backup path protection (BPP) based on MNP-e,where only a minimum number of links need to be protected, usingspecial paths and packet headers, to meet the network availabilityrequirement. We evaluate these algorithms in a wide spread ofrelevant topologies, both real and synthetic, and the results revealthat HLP can achieve high network availability without introducingconspicuous overhead. HLP not only needs around 10% time ofthat of full protection, but also provides full protection capabilitiesthat full protection provide.

Algebra and Algorithms for Multipath QoS Routing in Link State Networks

Haijun Geng,Xingang Shi,Zhiliang Wang,Xia Yin,Shaoping Yin 한국통신학회 2017 Journal of communications and networks Vol.19 No.2

The diversity of quality-of-service (QoS) requirementsof Internet applications motivates various QoS routing algorithmsthat take different QoSmetrics into consideration. Routing algebrahas been proposed as a framework to study the fundamental propertiesof QoS routing algorithms, such as their optimality and loopfreeness. However, for multipath QoS routing, little has been donein these aspects. Existing multipath QoS routing algorithms oftentake a rather conservative approach to guarantee loop-freeness, atthe cost of efficiency. On the other hand, simply adapting existingefficient multipath routing algorithms to support various QoSmetrics cannot guarantee correctness. In face of that, we proposea routing metric algebra for multipath QoS routing in link statenetworks, where a key property of the routing metrics called isotonicity,which plays an important role. To let routers efficientlyand correctly find multiple next-hops for each destination, we alsodevelop two distributed multipath QoS routing algorithms. Thealgorithms are run locally and independently, without exchangingmessages other than the basic link states. They are specifically tailoredfor algebras with strict or non-strict isotonicity, and their correctnessis formally proved.

Amino-modified carbon dots as a functional platform for drug delivery: Load-release mechanism and cytotoxicity

Nana Shi,Kaiyue Sun,Zedi Zhang,Juan Zhao,Lina Geng,Yuhua Lei 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.101 No.-

Carbon dot (CDs) were prepared for carrying DOX (Doxorubicin) or FAC (Ferric ammonium citrate), andthe loading mechanism and release kinetics of CDs-drugs were studied. CDs were prepared using peonypollen and urea, and characterized by TEM, XRD, 13C NMR, XPS, UV, FT-IR, DLS and zeta potential. Theobtained CDs were rich of –NH2 group on the surface, amorphous phase, spherical morphology withthe diameter about 5 nm, and had a blue fluorescence with the quantum yield (QY) of 12%. Iron disorderscan cause cancer or anemia. DOX is used for treating cancer, but has severe side effects. FAC is an ironsupplement agent, but had low bioavailability. CDs carried DOX (or FAC) through –N=C– (or –CONH–)bond, had high loading efficiency for DOX (37.2%) and FAC (54.0%) by optimizing mass ratios of mCDsto mdrug and reacted overnight. Cellular experiments showed that CDs-drugs had lower cytotoxicity thanfree drugs and CDs co-located with the drug. In vitro release indicated that CDs-DOX had pH-targetedproperty and CDs-FAC could be absorbed easily. CDs-drug release belonged to Weibull model, and releasemechanism was determined by the bonding way between CDs and drug. CDs had potential applicationsin delivery drugs with –C=O or –COOH group.

Excessive Daytime Sleepiness and Insomnia Symptoms in Adolescents With Major Depressive Disorder: Prevalence, Clinical Correlates, and the Relationship With Psychiatric Medications Use

Yudong Shi,Wei Li,Changhao Chen,Xiaoping Yuan,Yingying Yang,Song Wang,Zhiwei Liu,Feng Geng,Jiawei Wang,Xiangfen Luo,Xiangwang Wen,Lei Xia,Huanzhong Liu 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.11

Objective Excessive daytime sleepiness (EDS) and insomnia symptoms are common in patients with major depressive disorder (MDD), which might lead to a poor prognosis and an increased risk of depression relapse. The current study aimed to investigate the prevalence, and sociodemographic and clinical correlates of EDS and insomnia symptoms among adolescents with MDD.Methods The sample of this cross-sectional study included 297 adolescents (mean age=15.26 years; range=12–18 years; 218 females) with MDD recruited from three general and four psychiatric hospitals in five cities (Hefei, Bengbu, Fuyang, Suzhou, and Ma’anshan) in Anhui Province, China between January and August, 2021. EDS and insomnia symptoms, and clinical severity of depressive symptoms were assessed using Epworth sleepiness scale, Insomnia Severity Index, and Clinical Global Impression-Severity.Results The prevalence of EDS and insomnia symptoms in adolescents with MDD was 39.7% and 38.0%, respectively. Binary logistic regression analyses showed that EDS symptoms were significantly associated with higher body mass index (odds ratio [OR]=1.097, 95% confidence interval [CI]=1.027–1.172), more severe depressive symptoms (OR=1.313, 95% CI=1.028–1.679), and selective serotonin reuptake inhibitors use (OR=2.078, 95% CI=1.199–3.601). And insomnia symptoms were positively associated with female sex (OR=1.955, 95% CI=1.052–3.633), suicide attempts (OR=1.765, 95% CI=1.037–3.005), more severe depressive symptoms (OR=2.031, 95% CI=1.523–2.709), and negatively associated with antipsychotics use (OR=0.433, 95% CI=0.196–0.952).Conclusion EDS and insomnia symptoms are common among adolescents with MDD. Considering their negative effects on the clinical prognosis, regular screening and clinical managements should be developed for this patient population.

Cloning and Spatiotemporal Expression Analysis of Bombyx mori elav, an Embryonic Lethal Abnormal Visual Gene

Wang, Geng-Xian,Liu, Ying,Sim, Yang-Hu,Zhang, Sheng-Xiang,Xu, Shi-Qing Korean Society of Sericultural Science 2009 International Journal of Industrial Entomology Vol.18 No.2

Embryonic lethal abnormal visual (elav) is a lethal gene in Drosophila inducing the abnormal development and function of nervous system. We cloned a Bm-elav gene by bioinformatics and biological experiment, based on sequence of ELAV protein and dbEST of Bombyx mori. The full-length of Bm-elav cDNA is 1498 bp, contains a 906 bp open read frame (ORF) encoding a precursor of 301 amino acid residues with a calculated molecular weight of 34 kDa and pI of 8.99. Bm-ELAV protein precursor contains three RNA recognition motifs (RRM) in $24{\sim}91$, $110{\sim}177$ and $222{\sim}295$ bit amino acid residues respectively, and belongs to RNA-binding protein family. Bm-ELAV shared varying positives, ranging from 56% to 60% (Identities from 41% to 45%), with RRM from other species of Xenopus tropicalis, Apis mellifera, Tribolium castaneum, Branchiostoma belcheri and Drosophila. Gene localization indicated that Bm-elav is a single-copy gene, gene mapping within 12-chromosome from 7916.68 knt to 7918.16 knt region of nscaf2993. Spatiotemporal expressions pattern analysis revealed that Bm-elav expressed higher in most tested tissues and developmental stages in whole generation, such as silk gland, fat body, midgut, hemopoietic organ and ovary, but almost no expression in terminated diapause eggs. This suggested that the expression of Bm-elav in early developmental embryonic stages might induce abnormal development like in Drosophila. Cloning of the Bm-elav gene enables us to test its potential role in controlling pests by transferring the gene into field lepidopteran insects in the future.

A Unify Unbalance Compensation Strategy for Islanded Microgrid with Unbalanced Condition

Hongtao Shi,Fang Zhuo,Zhiqing Geng,Dong zhang 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

Due to the unbalance condition, the voltage of microgrid would be deteriorated. In this paper, a unify unbalance compensation strategy is proposed to suppress the unbalanced voltage and the voltage variation for islanded microgrid with unbalanced condition. Comparing to the traditional compensation strategy, where only negative sequence components of voltage are suppressed, in the proposed scheme, the voltage and frequency variations resulted from the unbalanced condition are also eliminated. A simulation model of microgrid is developed in PSIM and the simulation and experimental results verify the effectiveness of the proposed method.

Improvement on the Laminated Busbar of NPC Three-Level Inverters based on a Supersymmetric Mirror Circulation 3D Cubical Thermal Model

Feng-You He,Shi-Zhou Xu,Cheng-Fei Geng 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.6

Laminated busbars with a low stray inductance are widely used in NPC three-level inverters, even though some of them have poor performances in heat equilibrium and overvoltage suppression. Therefore, a theoretical method is in need to establish an accurate mathematical model of laminated busbars and to calculate the impedance and stray inductance of each commutation loop to improve the heat equilibrium and overvoltage suppression performance. Firstly, an equivalent circuit of a NPC three-level inverter laminated busbar was built with an analysis of the commutation processes. Secondly, on the basis of a 3D (three dimensional) cubical thermal model and mirror circulation theory, a supersymmetric mirror circulation 3D cubical thermal model was built. Based on this, the laminated busbar was decomposed in 3D space to calculate the equivalent resistance and stray inductance in each commutation loop. Finally, the model and analysis results were put into a busbar design, simulation and experiments, whose results demonstrate the accuracy and feasibility of the proposed method.

Research of 3D image processing of VR technology in medicine based on DNN

Gong ZhaoZhe,Li XiaoDong,Shi XiaoYing,Liu Geng,Chen Bin 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.5

According to a survey published in an authoritative journal in January 2020, the globalincidence rate of mental illness is 8.3% for men and 10.6% for women, which indicates thatmental illness has become a globally recognized obstacle. Therefore, specific psychotherapy including mental illness will become an important research topic. It is very effective forpatients with special mental diseases, such as mental illness, to reduce their mental reaction byexposure therapy; the system uses the virtual reality system of medical images processed by learningalgorithm to reproduce the effect of virtual reality exposure method of the high scene of transparent ladder. Compared with the old invasive exposure scene, the results show that theimprovement of both conditions has obvious effect, and the effect of human treatment underthe two conditions is not good. There are obvious differences, which show that virtual reality model will gradually replace the on-the-spot feeling. Finally, with more and more researchers have put forward a variety of other virtual reality image processing models, the research of image processing has gradually become more and more interested.