Collaborative fabrication of poly(L-proline)s with well-defined mesopores and hydrophobicity: Synergistic effect of mesoporous confinement and hydrophobic micro-environment on organic transformations

Zhongqiu Liu,Yuanyuan Yu,Shengnan Li,Yaqi Liu,Guoqiang Zhang,Long Han,Yujing Liu,Jinmao You,Anguo Ying 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.104 No.-

The preparation of materials with a well-defined mesoporous structure and hydrophobicity is of greatsignificance for heterogeneous catalyst development, yet there still remains a challenge for fabricatingthe above materials without using templates. Herein, the polymerization of L-proline-functionalizedmonomer with crosslinkers containing variable alkyl-bridged length (n = 2,4,6,8) and vinyl-modifiedFe3O4 nanoparticles to construct mesoporous poly(L-proline)s (MPLPs) has been reported for the firsttime. The resultant MPLPs were analyzed by characterization methods, suggesting that MPLPs with awell-defined mesoporous structure and unique hydrophobicity were successfully constructed by synergisticregulation of vinyl-modified Fe3O4 nanoparticles and crosslinkers with alkyl-bridged length of 6carbon atoms, in which vinyl-modified Fe3O4 nanoparticles as structural reinforcer plays a vital rolefor the mesoporous formation. Moreover, the resultant MPLPs were found to be efficient catalysts forone-pot synthesis of functionalized spiroindolones and olefins. The kinetic study and the water contactangle indicate the mesoporous confinement and hydrophobic micro-environment in organic transformations. Hence, the high activity of MPLPs is ascribed to synergistic effect of mesoporous confinement andhydrophobic micro-environment, and the adsorption experiment results further confirm this synergisticcatalysis. This work promotes a textural engineering approach to the development of multifunctionalmaterials with well-defined mesopores and hydrophobicity.

Ginsenoside Rk1 inhibits HeLa cell proliferation through an endoplasmic reticulum signaling pathway

Qiuyang Li,Hang Sun,Shiwei Liu,Jinxin Tang,Shengnan Liu,Pei Yin,Qianwen Mi,Jingsheng Liu,Lei yu,Yunfeng Bi 고려인삼학회 2023 Journal of Ginseng Research Vol.47 No.5

Background: Changes to work-life balance has increased the incidence of cervical cancer among youngerpeople. A minor ginseng saponin known as ginsenoside Rk1 can inhibit the growth and survival ofhuman cancer cells; however, whether ginsenoside Rk1 inhibits HeLa cell proliferation is unknown. Methods and results: Ginsenoside Rk1 blocked HeLa cells in the G0/G1 phase in a dose-dependentmanner and inhibited cell division and proliferation. Ginsenoside Rk1 markedly also activated theapoptotic signaling pathway via caspase 3, PARP, and caspase 6. In addition, ginsenoside Rk1 increasedLC3B protein expression, indicating the promotion of the autophagy signaling pathway. Protein processingin the endoplasmic reticulum signaling pathway was downregulated in Gene Ontology (GO) andKyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses, consistent with tealtimequantitative PCR and western blotting that showed YOD1, HSPA4L, DNAJC3, and HSP90AA1expression levels were dramatically decreased in HeLa cells treated with ginsenoside Rk1, with YOD1was the most significantly inhibited by ginsenoside Rk1 treatment. Conclusion: These findings indicate that the toxicity of ginsenoside Rk1 in HeLa cells can be explained bythe inhibition of protein synthesis in the endoplasmic reticulum and enhanced apoptosis, with YOD1acting as a potential target for cervical cancer treatment.

The Stress-Responsive and Host-Oriented Role of Nonribosomal Peptide Synthetases in an Entomopathogenic Fungus, Beauveria bassiana

( Hang Liu ),( Linan Xie ),( Jing Wang ),( Qiannan Guo ),( Shengnan Yang ),( Pei Liang ),( Chengshu Wang ),( Min Lin ),( Yuquan Xu ),( Liwen Zhang ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3

Beauveria bassiana infects a number of pest species and is known to produce insecticidal substances, such as the nonribosomal peptides (NRPs) beauvericin and bassianolide. However, most NRPs and their biological roles in B. bassiana remain undiscovered. To identify NRPs that potentially contribute to pathogenesis, the 21 predicted NRP synthetases (NRPSs) or NRPS-like proteins of B. bassiana ARSEF 2860 were primarily ranked into three functional groups: basic metabolism (7 NRPSs), pathogenicity (12 NRPSs), and unknown function (2 NRPSs). Based on the transcript levels during in vivo growth on diamondback moth (Plutella xylostella (Linnaeus)), half of the Group II NRPSs were likely to be involved in infection. Given that the metabolites biosynthesized by these NRPSs remain to be determined, our result underlines the importance of the NRPSome in fungal pathogenesis, and will serve as a guide for future genomic mining projects to discover functionally essential and structurally diverse NRPs in fungal genomes.

Mouse Fyn induces pseudopodium formation in Chinese hamster ovary cells

Lei An,Shengnan Liu,Wei Zhang,Yamei Zhang,Yingxue Huang,Xinde Hu,Shu-lin Chen,Shanting Zhao 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.1

Molecular mechanisms underlying the effects of Fyn on cellmorphology, pseudopodium movement, and cell migrationwere investigated. The Fyn gene was subcloned into pEGFP-N1to produce pEGFP-N1-Fyn. Chinese hamster ovary (CHO)cells were transfected with pEGFP-N1-Fyn. The expression ofFyn mRNA and proteins was monitored by reversetranscription-PCR and Western blotting. Additionally,transfected cells were stained with 4´,6-diamidino-2-phenylindole and a series of time-lapse images was taken. Sequences of the recombinant plasmids pMD18-T-Fyn andpEGFP-N1-Fyn were confirmed by sequence identificationusing National Center for Biotechnology Information in USA,and Fyn expression was detected by RT-PCR and Westernblotting. The morphology of CHO cells transfected with therecombinant vector was significantly altered. Fyn expressioninduced filopodia and lamellipodia formation. Based on theseresults, we concluded that overexpression of mouse Fyn inducesthe formation of filopodia and lamellipodia in CHO cells, andpromotes cell movement.

Preparations, application of polysaccharide–protein nanoparticles and their assembly at the oil–water interface

Hui Yang,Shengnan Wang,Lina Yang,He Liu 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.1

With the development of nanotechnology, nanoparticles have played an important role in pharmaceuticals, foods and materials, in particular, protein/polysaccharide based composite nanoparticles have received attention from researchers for safety and green production. This paper summarized in detail the preparation methods, applications of protein/polysaccharide nanoparticles (PPNPs) in recent years, especially the mechanism of stabilizing the oil–water interface. Currently, the polysaccharides applied are more traditional, such as chitosan, pectin and carboxymethyl cellulose, so there is still a lot of room for the development of raw materials that can be used to prepare PPNPs. Based on this, we also proposed three promising polysaccharides: seaweed polysaccharide, lycium barbarum polysaccharide and lactobacillus exopolysaccharides, describing their characteristics as well as their application prospects, this article can serve as a reference for interested researchers.

Output Feedback Passification for Switched Discrete-time Nonlinear Systems and Adaptive Control System Design

Hongbo Pang,Shengnan Tan,Shuo Liu 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.6

This paper concerns output feedback passification and adaptive output feedback stabilization problems of switched discrete-time nonlinear systems. First, passivity concept is proposed to describe the overall passivity of switched discrete-time nonlinear system without requiring the passivity of active subsystems. Then, the conditions to be passive for such system are provided. Second, the output feedback passification is achieved under a given switching signal, when switched zero dynamics system is passive. Furthermore, adaptive output feedback stabilization problem is solved by using the output feedback passification method. Third, a time-dependent switching signal is designed to guarantee switched zero dynamics system passive. Finally, two examples are presented to verify the effectiveness of the proposed methods.

Quantitative Proteomic Analysis Reveals Impaired Axonal Guidance Signaling in Human Postmortem Brain Tissues of Chronic Traumatic Encephalopathy

Baibin Bi,최한필,현승재,Shengnan Sun,Ning Su,Yuguang Liu,이정희,Neil W Kowall,Ann C McKee,Jing-Hua Yang,류훈 한국뇌신경과학회 2019 Experimental Neurobiology Vol.28 No.3

Chronic traumatic encephalopathy (CTE) is a distinct neurodegenerative disease that associated with repetitive head trauma. CTE is neuropathologically defined by the perivascular accumulation of abnormally phosphorylated tau protein in the depths of the sulci in the cerebral cortices. In advanced CTE, hyperphosphorylated tau protein deposits are found in widespread regions of brain, however the mechanisms of the progressive neurodegeneration in CTE are not fully understood. In order to identify which proteomic signatures are associated with CTE, we prepared RIPA-soluble fractions and performed quantitative proteomic analysis of postmortem brain tissue from individuals neuropathologically diagnosed with CTE. We found that axonal guidance signaling pathwayrelated proteins were most significantly decreased in CTE. Immunohistochemistry and Western blot analysis showed that axonal signaling pathway-related proteins were down regulated in neurons and oligodendrocytes and neuron-specific cytoskeletal proteins such as TUBB3 and CFL1 were reduced in the neuropils and cell body in CTE. Moreover, oligodendrocyte-specific proteins such as MAG and TUBB4 were decreased in the neuropils in both gray matter and white matter in CTE, which correlated with the degree of axonal injury and degeneration. Our findings indicate that deregulation of axonal guidance proteins in neurons and oligodendrocytes is associated with the neuropathology in CTE. Together, altered axonal guidance proteins may be potential pathological markers for CTE.

Highly flame-retarding cotton fabrics with a novel phosphorus/nitrogen intumescent flame retardant

Peihua Zhao,Yaqing Liu,Xinhang Li,Mei Zhang,Shengnan Liu,Wenjun Liang 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.9

A novel phosphorus/nitrogen intumescent flame retardant, namely 6-chloro-4-(diethylamino phosphoratephosphoryl chloride)-2-(sodium 4-aminobenzensulfonate)-1,3,5-triazine (APPCABT), has been synthesized and charac-terized by elemental analysis, FT-IR,1H-NMR and31P{H}-NMR spectroscopies. To investigate the flame-retardingproperties of the treated cotton fabrics, APPCABT was applied to the chemical treatment of the cotton fabrics surface. The flame-retarding performance of the treated samples with APPCABT was evaluated by limiting oxygen index (LOI). The thermal degradation behavior of the treated samples was studied using thermogravimetric analysis (TGA). Thesurface morphology of the treated samples was observed by scanning electron microscopy (SEM). These results showthat APPCABT is both an intumescent flame retardant with high efficiency and an excellent char-forming agent forthe flame-retarding treatment of cotton fabrics.

Sox12 Is a Cancer Stem-Like Cell Marker in Hepatocellular Carcinoma

Zou, Song,Wang, Chen,Liu, Jiansheng,Wang, Qun,Zhang, Dongdong,Zhu, Shengnan,Xu, Shengyuan,Kang, Mafei,He, Shaozhong Korean Society for Molecular and Cellular Biology 2017 Molecules and cells Vol.40 No.11

Recent studies on molecular carcinogenesis suggest that the chemo-resistance of some cancers is largely due to presence of cancer stem cells (CSCs), which affect the chemotherapy outcome for hepatocellular carcinoma (HCC). However, currently no consensus on a CSC phenotype in HCC has been obtained. Here, we examined Sox12 as a novel CSC marker in HCC. Sox12+ versus Sox12- cells were purified from HCC cell lines. The Sox12+ cells were compared with Sox12- HCC cells for tumor sphere formation, chemo-resistance, tumor formation after serial adoptive transplantations in nude mice, and the frequency of developing distal metastasis. We found that compared to Sox12- HCC cells, Sox12+ HCC cells generated significantly more tumor spheres in culture, were more chemo-resistant to cisplatin, were detected in circulation more frequently, and formed distal tumor more frequently. Moreover, Sox12 appeared to functionally contribute to the stemness of HCC cells. Thus, we conclude that Sox12 may be a novel marker for enriching CSCs in HCC.

A Fuzzy Cerebellar Model Articulation Controller Based Visual Servo System for Robot

Wei Sun,Cong Wang,Shengnan Liu,Baoqiang Wu,Minghua Ouyang 제어·로봇·시스템학회 2012 International Journal of Control, Automation, and Vol.10 No.2

This paper presents a novel online learning visual servo controller integrating the FCMAC with proportion controller for the control of position of manipulator end-effector. Since the FCMAC has good learning capability and fast learning speed, and can save much computer memory space by fuzzy processing of input space division and memory unit activation, it is used to develop an adaptive control law by learning the relationship between the image feature errors and manipulator input, and the aim of online learning of the FCMAC is to minimize the output of proportion controller. Further-more, the FCMAC has no need for models of robot manipulator and image feature extraction, so that the capability of proposed controller for tasks under uncertain environment can be improved. Finally, the proposed controller is proved to be effective by the experiment, and compared with BP neural net-work.