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유승희 ( Ryu¸ Seunghee ),황용섭 ( Hwang¸ Yongseop ),김주연 ( Kim¸ Jooyun ) 한국공간디자인학회 2021 한국공간디자인학회논문집 Vol.16 No.7
(연구배경 및 목적) 세계의 각 도시들은 대외적으로는 국가경쟁력 강화와 대내적으로는 지역 발전을 위해 적극적으로 도시 브랜딩을 활용하고 있다. 도시 브랜딩은 심볼, 로고 등 그래픽적 아이덴티티 확립에서 도시의 특색을 반영한 공간으로 그 범위가 확장되고 있다. 도시 브랜딩의 목적은 그 도시가 의도한 가치를 소비자들이 긍정적으로 인식하고 도시를 기억하여 재방문하도록 유도하는 데 있다. 이를 위해서는 소비자 입장에서 공간을 기억하는 가치기반의 관점에서 접근할 필요가 있다. 토포필리아는 공간과 장소의 개념을 분리하여 물리적인 환경인 ‘공간’이 공간에 대한 개인의 경험과 정서적 교류를 통해 특정한 ‘장소’로 인지된다는 이론이다. 이에 본 연구는 소비자 인식 가치에 기반한 토포필리아 관점에서 도시 브랜딩의 공간의 특성이 성공적인 도시 브랜딩 공간에서 어떻게 효과적으로 발현되고 영향을 미치는지 알아보고자 한다. (연구방법) 먼저, 토포필리아와 도시 브랜딩의 개념을 기존 문헌과 연구를 통해 재정립하고, 토포필리아와 도시 브랜딩의 특징을 도출하였다. 이를 바탕으로 토포필리아 관점에서 도시 브랜딩의 공간 특성을 도출하였다. 이후 도출한 특성을 중심으로 대상 공간을 선정하여 사례 연구를 진행한다. 대상 공간은 성공적인 도시 브랜딩의 공간 4곳을 선정하여 각 공간에서 도출된 특성이 어떻게 발현되는지 분석하였다. (결과) 본 연구에서는 토포필리아 관점으로서 도시 브랜딩의 공간 특성 중 정체성 지각과 상징적 태도의 역사성이 높게 나타났다. 그리고 상징적 태도의 상징성과 경험적 신뢰의 안전성에서 높게 나타났다. 이는 도시의 특색을 반영하고 잘 보존된 도시 브랜딩의 역사적 문화적 공간은 시각적 자극과 개인의 경험을 통해 특정 장소로 인식 전환에 긍정적인 영향을 주는 요소로 의미가 크다고 유추할 수 있다. (결론) 도시 브랜딩에서 공간은 시각적 자극과 동시에 경험을 통한 정서적 교류의 매개체이다. 기존 공간이 가지고 있는 역사적 환경적 요소를 정체성을 기반으로, 상징적으로 잘 보존하고 현대적으로 경험 가능한 공간으로 전략적으로 활용할 때 소비자들에게 가치 있는 특정 장소로 인식되고 오랫동안 기억될 수 있다. 이는 도시 브랜딩은 역사적 가치 공간을 잘 보존하고, 그 공간을 현대적으로 재해석해 적극적으로 활용하는 활동이 도시 브랜딩의 성공적인 결과로 귀결됨을 알 수 있다. 이를 위해서는 도시의 문화적 역사적 이해를 바탕으로 현대 소비자 가치기반의 니즈를 파악하여 과정을 수행할 때 도시 브랜딩의 공간으로 성공적인 결과를 가져올 수 있음을 유추할 수 있다. (Background and Purpose) Cities around the world are actively using urban branding to strengthen national competitiveness and internally develop the region. Urban branding is moving away from establishing graphic identities such as symbols and logos in spaces that reflect the city's characteristics. The purpose of urban branding is to encourage consumers to positively recognize the intended value and to remember the city and return. This requires a value-based approach that makes spaces memorable from a consumer’s perspective. Topophilia is the theory that "space", a physical environment that separates the concept of space and place, is perceived as a specific "place" through personal experiences and emotional exchanges. From a Topopilia perspective based on consumer awareness values, the study explores how the nature of urban branding effectively manifests and influences the characteristics of urban branding in a successful urban branding space. (Method) First, the concept of topopilia and urban branding was redefined through existing literature and research, and the characteristics of topopilia and urban branding were derived. Based on this, the spatial characteristics of urban branding were derived from a Topopilia perspective. The target space was selected based on the characteristics derived, and a case study was used to analyze how the characteristics derived from each space are expressed. (Results) In this study, the historicity of identity perception and symbolic attitudes was high among the spatial characteristics of urban branding from a Topopilia perspective. It also appeared high in the symbolism of symbolic attitudes and the safety of empirical trust. This reflects the city's characteristics and shows that the historical cultural space of well-preserved urban branding has a significant positive influence on changing perceptions through visual stimulation and personal experience. (Conclusions) In urban branding, space is a medium of visual stimulation and emotional exchange through experience. Based on the identity of the historical environmental elements of the existing space, it can be recognized and remembered as a specific place of value for consumers when strategically utilized as a symbolically well-preserved and modernly experienced space. It should be noted that urban branding preserves historical value spaces well, and actively using modern reinterpretations results in successful urban branding. This allows us to identify the needs of modern consumers’ values based on the cultural and historical understanding of a city; from this, we can infer that the process has a successful outcome.
Sugar Alcohol, Erythritol has Antioxidant Properties on Miniature Pig Sperm Cryopreservation
Seunghee Kim,Haein Kim,Gahee Kim,Wooyoung Park,Daeyoung Jeong,Wonjae Kim,Daeyoung Kim 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10
Cryopreservation of miniature pig sperm is essential because of high demand of organ transplant in mass production. However, miniature pig sperm are vulnerable to oxidative stress more than other mammals. Erythritol is a naturally occurring sugar alcohol with powerful antioxidant property. Thus, the aim of our study is to verify if erythritol could reduce lipid peroxide and enhance viability of frozen thawed miniature pig sperm. Ejaculated semen samples were frozen with cryoprotectant subjected to erythritol treatment (0, 10, 100, 500 mM). After frozen thawed, spematozoa viability were examined using the computer assisted sperm analysis (CASA) system. The product of lipid peroxidation, malondialdehyde (MDA) were quantified using spectrophotometer with DPPH and ABTS assays as ROS scavenger markers. Our result showed that erythritol enhanced sperm viability (p<0.05), reduced lipid peroxides significantly (p<0.05), proving the concentration of 100 mM erythritol to be an effective for lowing oxidative damage. Data from our study suggest that erythritol exhibits significant lipid peroxidation scavenging characteristics which may prevent oxidative damage, enhance viability of frozen thawed sperm and thus could be a effective additive as cryoprotectant.
Kim, Chanhoon,Choi, Sinho,Yoo, Seungmin,Kwon, Dohyoung,Ko, Seunghee,Kim, Ju-Myung,Lee, Sang-Young,Kim, Il-Doo,Park, Soojin RSC Pub 2015 Nanoscale Vol.7 No.26
<P>Conductive agent incorporating Si anodes consisting of directly grown carbon nanotubes on hard carbon encapsulating Si nanoparticles were prepared by a one-pot chemical vapour deposition process. Owing to this fabulous structure, Si-based anodes exhibit excellent cycle retention and rate capability with a high-mass-loading of 3.5 mg cm(-2).</P>
Establishment of bioassay system for RNAi-based control of Frankliniella occidentalis
Seunghee Han,Kyungmun Kim,Juhyeon Kim,Si Hyeock Lee 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.10
Frankliniella occidentalis is a notorious polyphagous crop pest causing tremendous economic loss. It damages flowers and leaves of host plants and also carries severe plant viruses. During last few decades, it has spread to all continents via transport of plant materials. Following extensive use of insecticides to control F. occidentalis, it has developed high level of resistance due to its short life cycle and high reproductive potential. In this study, RNA interference (RNAi)-based bioassay system was developed to find an alternative control measure for insecticide-resistant population of F. occidentalis. A variety of genes involved in various physiological mechanisms were selected for the test of dsRNA potency (tubulin, v-ATPase, amylase, aquaporin etc.). Each bioassay unit made by 3D printing has a leaf disc placed on 150 ㎕ of 50 ng/ul dsRNA solution and 20 thrips. The mortality was checked, and the dsRNA and leaf disc were replaced every 24 h for 72 h. Of the 20 genes tesetd, tubulin, v-ATPase, and aquaporin showed 31, 38, 38 and 45% of corrected mortality at 72 h post-treatment, respectively. This result suggests the potential of these genes as candidate lethal genes for RNAi-based F. occidentalis control system.
Kim, Chulki,Oh, Kiwon,Han, Seunghee,Kim, Kyungkon,Kim, Il Won,Kim, Heesuk American Scientific Publishers 2014 Journal of nanoscience and nanotechnology Vol.14 No.8
<P>The direct spontaneous grafting of 4-nitrophenyl molecules onto n-doped hydrogenated amorphous silicon (a-Si:H) surfaces without external ultraviolet, thermal, or electrochemical energy was invegtigated. Clean n-doped a-Si:H thin films were dipped in a solution of 4-nitrobenzenediazonium salts (PNBD) in acetonitrile. After the modified surfaces were rinsed, they were analyzed qualitatively and quantitatively by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). XPS and AFM results show that the reaction of an n-doped a-Si:H thin film with PNBD self-terminates without polymerization, after 5 h, and the surface number density of 4-nitrophenyl molecules is 4.2 x 10(15)/cm2. These results demonstrate that the spontaneous grafting of nitrophenyl layers onto n-doped a-Si:H thin films is an attractive pathway toward forming interfaces between a-Si:H and organic layers under ambient conditions.</P>