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Examining Korean English Teachers’ Question-in-Interaction with Young Learners
Wooyoung Park 한국응용언어학회 2014 응용 언어학 Vol.30 No.3
This study investigates how English teachers who are non-native speakers (NNS) produce their questioning practices according to different contexts of the classroom setting. Four female teachers working in Seoul and Gyeonggi Province participated in the data collection. For each teacher, four sessions of English lessons were recorded via audio or video and transcribed respectively by following the Jefferson system. Conversation analysis (CA), specifically sequential analysis, was used as the main method for data analysis in this study. The findings of the present study indicate that both NNS teachers and students alike make full use of sequential turns as resources for their talks, as well as the functions and forms of L2. The implications are significant in that teachers’ questions are not merely used as interrogation, but utilized to foster interactivity among teachers and students. More importantly, knowing the forms and functions of L2 should be juxtaposed with using them in actual interaction. Accordingly, this provides the stakeholders of teacher education curriculum with an emphasis that requires prospective teachers to improve awareness about how to produce teacher talk-ininteraction.
Wooyoung PARK,Ji-Sook HAHN 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Methane has emerged a promising alternative carbon source to produce valuable chemicals. To produce lactic acid from methane, we developed lactate-tolerant methanotroph strain by adaptive evolution of Methylomonas sp. DH-1. The evolved strain survived up to 10 g/L lactate, whereas wild type could not grow even in the presence of 1 g/L of lactate. Whole genome sequencing of the evolved strain revealed a point mutation in the alaRS gene encoding alanyl tRNA synthetase. Introduction of the alaRS mutation into the wild-type strain led to an increase in lactate tolerance, suggesting this point mutation is responsible for the lactate tolerance. AlaRS catalyzes the charging of Ala to tRNAAla, but the enzyme can also mischarge Ser or Gly, which is subsequently removed by an editing activity of AlaRS. A defect in editing activity was shown to be detrimental in bacteria and humans even with very small amount of mutant proteins with Ala to Ser mutation. We identified that the identified point mutation in the catalytic domain of alaRS could reduce Ser mischarging while increasing Ala-charging activity. These results suggest that increased fidelity of alaRS activity may lead to specific changes in proteome, which in turn may increase lactate tolerance of Methylomonas sp. DH-1
Park, Wooyoung 한국실천신학회 2011 신학과 실천 Vol.0 No.29
The article starts with a problem-posing of how Christians understand complex reality of the current society and respond to it. In many cases, many young adult members in the context of Korean churches are asked to just ignore harsh realities of the society with so-called “faith.” In this article, thus, we need to help young adult lay members to analyze and interpret what is going on in the society and critically raise a question of what Christian responsibility may look like in the era of globalized society. To help them to participate in acting toward a more just society, a practical class for Christian social engagements can be proposed, which includes the following sessions of 1) Christian economic life, 2) critical issues of GMOs in our life, 3) power relationships in cyberspace, and 4) making connections among social issues and consciousness. In the consecutive sessions, there are four important focal points emphasized and shared with each other: 1) social and ecological values beyond monetary values, 2) intrinsic values of all beings and current life-manipulations in the globalized economy, 3) relocation of ourselves in the web of interrelations, and 4) possible actions and practices for a more just society. These practical class sessions motivates young adult lay members to responsibly live their Christian life based on what they believe and what they learn. In this project of lay member’s class, we come to know that teaching and learning as a whole for Christian social engagements is a continual process of realizing what we believe for correcting, motivating, and sustaining our interrelations around and among us toward a more just society.
Park, Min Sun,Lee, Jun Ho,Park, Yunji,Yoo, Ran,Park, Seungryol,Jung, Hwaebong,Kim, Wonkyung,Lee, Hyun-Sook,Lee, Wooyoung Elsevier 2019 Sensors and actuators. B, Chemical Vol.299 No.-
<P><B>Abstract</B></P> <P>We report on the doping effect on the sensing properties of ZnO quantum dots (QDs) for the detection of acetylene. We found that In-doped ZnO (IZO) QDs exhibited a better sensing performance to 10 ppm acetylene than undoped ZnO (ZO) QDs and Al-doped ZnO (AZO) QDs. The higher sensing response of IZO QDs can be attributed to a greater number of reactive sites for detecting acetylene, which is likely to originate from the increased number of oxygen vacancies, and the larger optical band gap and surface area of IZO. This is due to a higher valence dopant and a smaller particle size. The sensing properties of IZO QDs to 10 ppm acetylene was also found to be superior to previously reported acetylene sensors that are based on semiconducting metal oxides. Furthermore, we demonstrated that 10 ppm of acetylene can be selectively detected in air within ∼100 s using a recently developed miniaturized gas chromatography (GC) integrated with the IZO QDs sensor. In addition, we found that the device can detect the major fault gases of hydrogen and acetylene separately within ∼100 s. Our study demonstrates that the device can be utilized in the GC-based on-line dissolved gas analysis to detect small amounts of acetylene gas in transformer oil.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We report the doping effect on the sensing properties of ZnO quantum dots for acetylene. </LI> <LI> Doping of ZnO quantum dots modified oxygen vacancies and optical band gaps. </LI> <LI> In-doped ZnO quantum dots exhibited a better sensing performance to 10 ppm acetylene. </LI> <LI> 10 ppm acetylene could be selectively detected using a miniaturized gas chromatography. </LI> <LI> The device can detect the major fault gases of hydrogen and acetylene separately within ∼100 s. </LI> </UL> </P>
Park Gaeun,Jang Wooyoung Eric,Kim Seoyeon,Gonzales Edson Luck,Ji Jungeun,Choi Seunghwan,Kim Yujin,Park Ji Hwan,Mohammad Hazara Begum,Bang Geul,Kang Minkyung,Kim Soobin,Jeon Se Jin,Kim Jin Young,Kim Kw 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Autism spectrum disorder (ASD) is a neurodevelopmental disorder associated with impaired social behavior and communication, repetitive behaviors, and restricted interests. In addition to genetic factors, environmental factors such as prenatal drug exposure contribute to the development of ASD. However, how those prenatal factors induce behavioral deficits in the adult stage is not clear. To elucidate ASD pathogenesis at the molecular level, we performed a high-resolution mass spectrometry-based quantitative proteomic analysis on the prefrontal cortex (PFC) of mice exposed to valproic acid (VPA) in utero, a widely used animal model of ASD. Differentially expressed proteins (DEPs) in VPA-exposed mice showed significant overlap with ASD risk genes, including differentially expressed genes from the postmortem cortex of ASD patients. Functional annotations of the DEPs revealed significant enrichment in the Wnt/β-catenin signaling pathway, which is dysregulated by the upregulation of Rnf146 in VPA-exposed mice. Consistently, overexpressing Rnf146 in the PFC impaired social behaviors and altered the Wnt signaling pathway in adult mice. Furthermore, Rnf146-overexpressing PFC neurons showed increased excitatory synaptic transmission, which may underlie impaired social behavior. These results demonstrate that Rnf146 is critical for social behavior and that dysregulation of Rnf146 underlies social deficits in VPA-exposed mice.
Production of Ebola virus-like particles in <i>Drosophila melanogaster</i> Schneider 2 cells
Park, Eun-Mee,Park, Sun-Whan,Lee, Ye-Ji,Lee, Won-Ja,Choi, Wooyoung Elsevier 2018 Journal of virological methods Vol.261 No.-
<P><B>Abstract</B></P> <P>In this study, we generated recombinant virus-like particles (VLPs) against family <I>Filoviridae</I>, genus <I>Ebolavirus</I>, species <I>Zaire ebolavirus</I>, strain <I>Makona</I> (EBOV) in <I>Drosophila melanogaster</I> Schneider 2 (S2) cells using the EBOV <I>Makona</I>. S2 cells were cotransfected with four viral plasmids encoding EBOV Makona proteins and protein expression was analyzed by immunoblotting. We confirmed that EBOV <I>Makona</I> proteins were successfully expressed in S2 cells. Additionally, we further examined the formation of intracellular and extracellular VLPs by electron microscopy. eVLPs were produced by sucrose gradient ultracentrifugation of S2 cells transfected with EBOV <I>Makona</I> genes, and production of VLPs was confirmed by immunoblot analysis. Collectively, our findings showed that the S2 cell system could be a promising tool for efficient production of eVLPs.</P>
Image-based Force Estimation of Deformable Tissue using Depth Map for Single-Port Surgical Robot
Wooyoung Kim,Sungmin Seung,Hongseok Choi,Sukho Park,Seong Young Ko,Jong-Oh Park 제어로봇시스템학회 2012 제어로봇시스템학회 국제학술대회 논문집 Vol.2012 No.10
This paper explains an image-based force estimation of deformable tissue for a small single-port surgical robot. Since the end-effectors of a single-port surgical robot are thin and slender, it is one of challenging issues to install a force-torque sensor on its tip. Instead of using a force sensor, image-based estimation of interacting forces between deformable tissue and surgical end-effectors are proposed in this paper. For this purpose, a depth map extracted from stereo-endoscopic images is compared with an original shape of the deformable tissue which is obtained from the pre-operative medical images. To mitigate computational burden and to distinguish interaction forces of two end-effectors, subsets of whole nodes near contact points are utilized to calculate the interaction forces. This image-based force estimation will be integrated with the developed single-port neurosurgical robotic system to show its efficiency and performance.