Diatomaceous earth를 이용한 경제적이고 효율높은 homemade PCR purification column의 제조 및 평가

이동호,이승관,이창규,조경진,최명재,이국성 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.2

In recent years the amount of sequencing has increased tremendously, especially due to the introduction of automated DNA sequencing. PCR is principal method for the synthesis of templates for DNA sequencing. Consequently, the need for PCR cleanup for template preparation has increased. Already, many PCR purification kits are marketed. This DNA purification kits uses most glass membrane or silica gel, So, can do rapidly efficient purification by simple miniprep. But, efficiency of purification kits is different according to manufacturer and is very expensive. Therefore, we compare the homemade Diatomaceous earth column with major manufacturer's spin column methods for DNA purification. As a result, we found that the homemade Diatomaceous earth column method resulted in equal or better yields relative to the commercial purification kits.

체중감량기간이 태권도선수들의 무산소성파워 및 심폐기능에 미치는 영향

이승국,이경배 한국체육대학교 2001 論文集 Vol.24 No.-

The purpose of this study was to determine the effects of period of weight loss on physiological factors in taekwondo players. This study employed 10 taekwondo players who were 17∼19 years old. The subjects were randomly assigned to one of two groups (one is experimental group and the other is control). Each group ended up with 5 subjects. The analysis of this study utilized two way ANOVA design. The results of the study were as follows : 1.There were no difference in anaerobic power and lactic acid betweenr groups 2.Before and after weight loss, cardiovacular fitness was much more deceased than group who was not state of weight loss

송전선로 보호계전기 정정값 진단 시스템

이승재,김국진,최면송,강상희 明知大學校 産業技術硏究所 2000 産業技術硏究所論文集 Vol.19 No.-

In the point of view a protective relay, the selectivity and sensitivity is very important in its performance. The correct setting of the relay parameter is required. The verification of accurate setting for the protective relay is very difficult before a actual fault occur. In this paper, we proposed the diagnosis expert system as a method to verify the correctness of the relay setting. The developed system proved effectiveness through the tests on the real systems.

리듬운동이 아동들의 체력향상에 미치는 영향

이상헌,이성국 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.2

This study used two group in order to recognize a physical strength elevation and a class preference duty of kindergarten children and did a comparative study. selects 60 control groups and 60 experiment groups, and the control group executed only kindergarten class, and an experiment group executes 2 times a week physical education classes according to a physical education program to 40 minutes in kindergarten class time Investigation analyzed a physical change and an elevation of physical strength of kindergarten children, a class preference duty of kindergarten child. First, a child of rhythm sports group is displaying growth in a development of a build than a child of similar times uniformly, displays a fast listed development in a kidney development in rhythm sports group. Second, than a group general physical strength II in rhythm sports group displays effective rise in force of a core and stamina of a core there was not a large difference, but synergy effect broke out in a flexibility development high than a general group. Third, general children prefer physical education (outdoor game), a computer, art and neck in comparison class preference, physical education, art, music, a computer appeared in rhythm sports group by preferring a course with a lot of movements.

리더십기능이 체육학습에 미치는 효과

이상연,육조영,이성국 한국스포츠리서치 2001 한국 스포츠 리서치 Vol.12 No.4

The purpose of this study was to investigate the relationship between teacher's leadership and task environment recognized by learners and the effect of leadership of sub-group in the class upon organizational behavior. It meant to examine the instructional activity and learning activity from the standpoint of leadership theory. The subjects 139 pupils. 1. Teacher's leadership of task orentation decreased uncertainty of expectancy which conditioned task environment. 2. Teacher's leadership of human orientation and task orientation enhanced valence of learning activity and outcome. 3. Significant positive correlation between sub-group's leadership human orientation and learning effectiveness in favorable task environment for learners were found(p<0.1). 4. Significant positive correlation between sub-group sleadership of human orientation (p<.01) and task orientation (p<.05) and recognition of pleasure in sport were found in favorable task environment

삼각측량 시스템을 이용한 물체의 3-D 정보추출 관한 연구

이호영,김국세,문정환,이철승,방극인,박영옥 조선대학교 전자정보통신연구소 2002 電子情報通信硏究所論文誌 Vol.5 No.2

The 3-D shape use to effect of movie, animation, industrial design, medical treatment service, education, engineering etc... But it is not easy to make 3-D shape from the information of 2-D image. There are two methods in restoring 3-D video image through 2-D image; First the method of using a laser; Second, the method of acquiring 3-D image through stereo vision. Instead of doing two methods with many difficulties, I study the method of simple 3-D image in this research paper. We present here a simple and efficient method, called direct calibration, which does not require any equations at all. The direct calibration procedure builds a lookup table(LUT) linking image and 3-D coordinates by a real 3-D triangulation system. The LUT is built by measuring the image coordinates of a grid of known 3-D points, and recording both image and world coordinates for each point; the depth values of all other visible points are obtained by interpolation.

여자 대학생의 에어로빅댄스 참여가 사회 심리학적건강에 미치는 영향

이석인,이성국,김제영 중앙대학교 스포츠과학연구소 2002 體育硏究 Vol.- No.16

This purpose of this study was to investigate the effect of dance sport participation on the mental head leisure satisfaction. For this purpose, through 80 females were selected among the J university. For analysis, the sub-program of SPSS V. 10.0 were used: independent t-test, One-way ANCOVA was use mental health and leisure satisfaction. The con clusion of this study are as follows. 1. The aerobic dance group shows higher self-esteem compared to the control group. 2. The aerobic dance group shows higher life satisfaction compared to the control group. 3. The aerobic dance group shows lower melancholy compared to the control group. 4. The aerobic dance group shows lower anxiety compared to the control group. 5. The aerobic dance group shows higher leisure satisfaction compared to the control group.

여자 태권도 선수의 경기 시 선제득점이 경기결과(승 ·�)에 미치는 영향

이승국,문원재,양대승 韓國體育大學校 1999 論文集 Vol.22 No.-

As the results of 504 taekwondo games in women during 1998∼1999 national representative selection tournaments were analyzed for knowing early earned points in which excellent women taekwondo players were participated by weight devision, competition round, and pattern of attacking kicks in taekwondo. The results were as followed. The average % of victory and defeat in fin weight was 86.1% and among the competition rounds, third round was the 92.9 % of victory and defeat and in case of attack type, first attack was the 94.6 % of victory and defeat and there were no significantly different among rounds but there was significantly different among attack types (p<.05) The average % of victory and defeat in fly weight was 77.6% (short distance attack, 83.3%), that of bantam weight is 93.3% (first round, 96.9%, short distance attack, 100%), that of ferther weight is 82.1% (second round, 94.1%, short distance attack, 100%), and there were no significantly different among rounds and attack types. The average % of victory and defeat in light weight was 74.6% (short distance attack, 83.8%), that of welter weight is 76.2% (first round, 96.9%, short distance attack, 100%), that of middle and heavy weight is 86.0% and 81.0% respectively. (each third round, 100%, short distance attack, 100%), and there were no significantly different among rounds and attack types.

임상병리검사업무의 전산화에 관한 연구

이승국,유영선 대한임상병리사협회 2001 대한임상병리사회지 Vol.21 No.1

아미노산 투여가 운동중 싸이클선수의 젖산 및 심박수에 미치는 영향

이승국,유병렬,김문희,김완조,이선장 韓國體育大學校附屬 體育科學硏究所 1992 韓國體育大學校附屬 體育科學硏究所論文集 Vol.11 No.1

The purpose of the study was to determine whether a 15.6mg.Kg ??' does of glutamate given one hour prior to incremental exercise test would reduce the lactate formation. Five well trained cyclists served as subjects in this experiment which included a placebo and glutamate trial. The subjects initially work on treadmill 200m/min for 3 minutes and here increased the workload by 20m/min every 3minutes until exhaustion. Blood lactate were collected from finger-tip. Subjects in the glutamate trial showed significantly lower lactate formation during exercise then they did in the placebo trial. In both groups no significant difference was found on heart rate. During the recovery lactate and heart, rate were unaltered in either the glutamate or placebo trials. In summary, glutamate ingestion may reduce lactate formation resultly improvement of performance.