김치식이가 흰쥐의 항산화시스템에 미치는 영향

류승희,권명자,송영옥,송영선,문갑순 인제대학교 2003 仁濟論叢 Vol.18 No.1

김치는 배추를 주재료로 하여 마늘, 고추, 생강 등의 양념류를 포함하는 여러 재료들로 만들어지며 이들 재료 중에는 항산화효과를 가진 것으로 알려진 성분들이 많이 함유되어 있어 김치도 항산화효과를 나타낼 것으로 여겨진다. In vitro 실험계 및 세포 모델계에서 김치 재료 및 김치의 항산화효과는 확인된바 있으나 In vivo 시스템에서의 김치의 항산화효과에 관한 자료는 부족하다. 따라서 본 실험에서는 김치 섭취의 생체 내 항산화효과를 규명하기 위하여 흰쥐에게 동결건조한 김치 3, 5, 10% 첨가 식이를 6주간 섭취시키면서 간조직의 산화와 항산화효소계의 활성을 측정하였다. 간의 MDA 함량은 대조군에 비해 김치 투여군에서 낮았고 특히 5% 김치섭취군에서 현저히 낮은 값을 나타내었다. 단백질 산화를 나타내는 카르보닐 함량도 대조군에 비해 김치섭취군에서 낮아 생체 내에서 김치식이가 과산화물 생성 억제효과를 나타내었다. 항산화 효소계의 활성을 측정한 결과 SOD 활성은 대조군에 비해 김치섭취군에서 현저하게 높았고 김치첨가 함량이 증가할수록 SOD 활성이 유의적으로 높아져 10% 김치섭취군에서는 대조군에 비해 70% 이상 높은 활성을 나타내었다. Catalase 활성은 각 군에서 유의적인 차이가 나타나지 않았으나 GSH-px의 활성은 김치 섭취가 높을수록 상대적으로 높은 증가율을 나타내어 SOD 활성과 동일한 경향을 나타내었다. 따라서 김치식이는 생체 내에서 치질 및 단백질 과산화를 현저하게 억제하였고 항산화효소계인 SOD와 GSH-px의 활성을 농도의존적으로 증진시켜 김치식이가 체내의 산화적 스트레스를 효과적으로 억제하는데 기여함을 알 수 있었다. Kimchi is a Kruean traditional fermented food containing various ingredients such as Chinese cabbage, garlic, ginger, red pepper and fish sauce. Some of their components were known as its antioxidative activities and antioxidative effects of kimchi have been studied using in vitro and cell model system. To investigate the antioxidative effect of dietary kimchi, 4 groups of male S.D. rats (n=36) were fed 0, 3%, 5%, and 10% freeze-dried kimchi for 6 weeks and hepatic lipid peroxidation, protein oxidation and the activities of antioxidative enzymes were measured. Hepatic MDA content fed kimchi diet was lower than control diet. Protein carbonyl values were decreased in kimchi diet group, but not significant. SOD activities were significantly increased in kimchi added groups at dose-dependent manner. At 10% kimchi added group, SOD activity increased by 70% compared with control diet group. Catalase activities were not different between experimental groups and control group, whereas GSH-px activities were significantly increased in kimchi added group. As a result kimchi diet inhibited lipid and protein oxidation and induced antioxidative enzymes, SOD and GSH-px in rat hepatic tissue. It suggests that dietary kimchi is expected to prevent the oxidative stress in animal and human.

가역 투자율 측정에 의한 1Cr-1Mo-0.25V 강의 열화도 평가

유권상,김용일,남승훈,유광민,조육,손대락 한국비파괴검사학회 2000 한국비파괴검사학회지 Vol.20 No.5

고온에서 운용 중인 설비의 안전성을 평가하기 위해서는 사용기간동안 열화된 재료의 물성을 측정하여야 한다. 실제 사용되고 있는 화력발전소 터빈의 로터에서 열화도가 다른 여러 종류의 시편을 입수하기 어렵기 때문에, 터빈 로터재로 널리 사용되고 있는 1Cr-1Mo-0.25V 강을 인공열화시켜 시편으로 사용하였다. 열화도의 비파괴적 평가를 위하여 교류 섭동 자기장을 인가하여 가역 투자율을 측정하는 자기적 방법을 사용하였다. 열화도의 증가에 따라 경도와 가역 투자율 피크 사이의 간격은 감소하였는데, 경도와 가역 투자율 피크 간격과의 선형관계를 이용하여 비파괴적으로 터빈 로터강의 열화도를 평가할 수 있는 기초를 마련하였다. The integrity of the turbine totors can be assessed by measuring reversible permeability and Vickers hardness of the aged rotors at service temperature. The measurement system of reversible permeability, which measured by applied alternating perturbing magnetic field, was constructed in order to evaluate matrial degradation, nondestructively. The test specimen was 1Cr-1Mo-0.25V steel used widely for turbine rotor material, and the specimens were prepared by the isothermal heat treatment at 630 ℃. The reversible permeability of the test materials were measured at room temperature. The peak interval of reversible permeability and Vickers hardness decreased with the increase of degradation. The degradation of test material may be determined nondestructively by the lineality of Vickers hardness and the peak interval of reversible permeability.

신라대학교 주변 백양산의 동물상

노치권(외 6명) 신라대학교 자연과학연구소 2012 自然科學論文集 Vol.22 No.-

To study fauna of Silla University located in Mt. Baek-yang, we conducted six surveys from 29 May 2011 to 28 April 2012. We found five phyla 41 orders 197 species of animals inhabiting in this area: four phyla 27 orders 163 species of invertebrates and in vertebrates one order six species of fishes, three orders nine species of amphibians and reptiles, six orders 14 species of aves, and four orders five species of mammals. Of these species, some Korean endemic species were included: a fish of Rhodeus uyekii in a pond near main gate of this university, a frog of Rana coreana in a creek near a dormitory, and a rabbit of Lepus coreanus and a water deer of Hydropotes inermis in a grass region near a main stadium. A kind of hawk, Falco tinnunculus as a natural monument (animal) of no. 323-8, was observed on a building of medical and life science. A blue frog of Hyla japonica as a variation was found near a spring resort. Therefore, this area is considered an ecologically important area having a high animal diversity.

안내염과 대장암이 동반된 재발성 간농양

전승정,김태헌,류민선,오다연,송명은,이신아,류재인,김혜인,문일환,유권 이화여자대학교 의과학연구소 2011 EMJ (Ewha medical journal) Vol.34 No.2

The causes of pyogenic liver abscess has been known as biliary tract disease or intrabadominal infection but the large proportions of the patients has no apparent underlying disorders. Recently colonic mucosal lesions were reported in patients with cryptogenic liver abscess and it has been suggested that colonic mucosal break may play a role in developing liver abscess in otherwise healthy patients. We experienced a patient of severe recurrent liver abscess complicated with endophthalmitis only 3 months after successful treatment of initial cryptogenic liver abscess and a polypoid colon cancer was discovered by chance. It seems prudent to proceed colonoscopic examination in patients with cryptogenic liver abscess especially when it is recurrent.

견관절 재발성 탈구에서 관절경적 견관절 전방 고정술의 결과 : 2년에서 6년간의 추시 관찰 Two to Six-Year Follow-up

김승호,하권익,노규철,류병담,오일빈 대한스포츠의학회 2003 대한스포츠의학회지 Vol.21 No.1

The purposes of this study were to prospectively evaluate the surgical otucome of the arthroscopic repair of the capsulolabral lesion using suture anchors in al large series of patients who were followed-up for mid-term periods. We evaluated 167 patients with traumatic recurrent anterior shoulder instability after an arthroscopic Bankart repair using suture anchors and nonabsorbable sutures. The mean age at the time of the operation was 25 years (15~46 years). There were 149 male and 18 female patients. Preoperatively and at the time of follow-up(mean, 44 months; range, 24~75 months), three objective outcome measurement tools(Rowe Score, University of California at Los Angeles Shoulder rating scale, and American Shoulder and Elbow Surgeons score) and two subjective measurements(pain and function visual analogue scale) sere assessed. The recurrence rate, range-of-motion, and risk factor for postoperative recurrence were evaluated. All shoulder scores improved after surgery(t-test, p<0.05). According to the Rowe scale, One hundred and thirty patients(78%) showed excellent, 29(17%) good, 6(4%) fair, and 2(2%) poor scored. Overall, postoperative recurrence of the instability was 4%(1 dislocation, 2 subluxation, and 4 positive anterior apprehensions). Postoperative recurrence was related with the glenoid defect of greater than 30%(Gehan's test, p<0.0001). In patients with postoperative instability, the recurrent episode was less frequent and shoulder function was related with the activity were increased after the surgery(t-test, p<0.001). One hundred and fifty-two patients(91%) returned to more than 90%of previous activities. The mean loss of external rotation was 2.0±4.0℃. In contrast to the previous reports on the results of the arthroscopic repair, arthroscopic capsulolabral repair using suture anchors provided satisfactory outcomes in terms of the recurrence, activity, and range of motion.

2차원 스토커 소각론의 SNCR 탈질기술개발에 대한 수치해석

신미수,유승민,유재관,장동순,김동찬 충남대학교 환경문제연구소 2000 環境硏究 Vol.18 No.-

In this study was the stoker incinerator system with SNCR(Selective Non-Catalytic Reduction). While this system was favored with the view of the possibility of the reducing waste volume and recovery of waste heat, it also has a disadvantage, that is, the need for the second to treat dust, HCl, CO, etc. So far, even though a great deal of research had been done, the study on heat-flow analysis with reduction model and NO reduction with SNCR had not been investigated enough. The temperature distribution in the system and excess amount of oxygen influence the efficiency of NO reduction, resolution in the production, resolution in the production of NO at 1700K and the temperature range of 1200-1250K when the highest NOx reduction efficiency occurred. Hence, this study was focused to flow mixing pattern and NOx reduction efficiency as the flue gas velocity, NH3 injection velocity, particle size and particle density.

측정방법에 따른 콩 성분의 항산화효과 비교

문갑순,권태완,류승희 한국콩연구회 2003 韓國콩硏究會誌 Vol.20 No.1

콩 성분의 항산화효과를 측정을 위해 DPPH법, FRAP법, TEAC법을 이용하여 이들 방법간의차이를 비교하고, 콩의 항산화효과를 측정하는데 가장 적합한 실험방법을 확립하고자 하였다. 콩의 유효 성분 중 항산화효과와 관련있는 isoflavone 및 phenolic acid의 표준품을 이용하여 항산화효과를 측정하였고, 항산화효과의 비교를 위해 Trolox, ascorbic acid, glutathione의 활성을 측정하였다. DPPH법, FRAP법, TEAC법의 3가지 항산화효과 측정방법에 따른 genistein의 효과가 가장 높았고 DPPH나 FRAP의 경우는 gentisic acid, syringic acid, ferulic acid 등의 phenolic acids의 활성이 높게 나타났고 isoflavones의 효과는 매우 낮게 나타났다. 항산화효과를 측정하기 위해 다양하게 이용되고 있는 방법들간에는 차이가 있음을 알 수 있었으며 TEAC법의 경우 glutathione 및 콩의 주요 성분인 isoflavones과 대부분의 phenolic acids의 활성을 평가할 수 있어 유용한 방법으로 여겨진다. To establish the proper assay for measurement of antioxidative activities in soybean, the main components relating to their antioxidative effects were measured by DPPH (α, α-diphenyl-β-pycryl-hydrazyl radical), FRAP (ferric reducing/antioxidant power), and TEAC (Trolox equivalent antioxidant capacity) assay. The antioxidative activities of commercial isoflavones (genistein, daidzein, and glycitein) and phenolic acids, which were well known as main antioxidative ingredients in soybean, were measured by three methods and compared the activities with Trolox, ascorbic acid, and glutathione as reference antioxidnats. By the FRAP and DPPH assay, gentisic acid, syringic acid and ferulic acid showed the strong antioxidative activities, while isoflavones and glutathione showed very low or undetectable activities . In contrast genistein, p-coumaric acid, and ferulic acid showed remarkable antioxidative activities by the TEAC assay. Therefore. it is proposed that TEAC assay is suitable for detecting antioxidative activity of soybean.

수증기 활성화법으로 제조된 영월 석탄계 활성탄의 특성 연구

이송우,권태훈,나영수,최동훈,류동춘,송승구 한국환경과학회 2000 한국환경과학회지 Vol.9 No.4

Activated carbons were prepared from Youngwall coal by steam activation in this study. The feasibility of the Youngwall coal to commercial activated carbon was examined. The variation of pore structures and the development of porosity in activated carbons were investigated by changing activation conditions in batch type apparatus. The values of BET surface area and adsorption capacity of iodine and methylene blue of the resulting activated carbons were obtained as high as 1,000㎡/g, 900㎎/g, 150㎖/g, respectively. Youngwall activated carbon prepared in this study showed much higher pore volume in pore diameter over l0Å than that of commercial reference activated carbon(Ningxia Taihua ZJ-15C) produced from China anthracite.

忠州市 地域特性을 反暎한 共同住宅 計劃硏究 : 충주지역 거주자의 APT 선호특성을 중심으로

朴碩鉉,朴義權,柳顯紀,尹勝照,孫泰鎭,金基洙 충주대 2003 産業科學論文集 Vol.11 No.-

The purpose of this research is to analyze the correlation of the characteristics of dwellers and residential preference in apartment housing through the case study of 340 samples in 25 housing estates which were built in Chung-ju city The contents of this research consist of two main parts. The first part is to identify characteristics of dwellers, which might have an influence on residential preference in apartment housing. The second part is to identify the main physical elements of residential preference that can be influenced by the personal characteristics. Thus, this research will contribute to the establishment of the planning methods which increase residential preference in apartment housing, while promoting the quality of residential environment in Chung-ju city.

Quassinoids isolated from <i>Brucea javanica</i> inhibit pepper mottle virus in pepper

Ryu, Seung Mok,Kwon, Jaeyoung,Seo, Young Hye,Song, Eun Gyeong,Hong, Seong Su,Kim, Beom Seok,Hong, Jin Sung,Ryu, Ki Hyun,Lee, Dongho ELSEVIER 2017 VIRUS RESEARCH Vol.227 No.-

<P><B>Abstract</B></P> <P>A green fluorescent protein (GFP)-tagged pepper mottle virus (PepMoV) based leaf-disc method and systemic host method were developed to identify antiviral agents. Preliminary experiments using a PepMoV-GFP based leaf-disc method led to the isolation of five quassinoids, including brusatol (<B>1</B>), bruceantin (<B>2</B>), brucein A (<B>3</B>), bruceantinol (<B>4</B>), and brucein B (<B>5</B>), from the CH<SUB>3</SUB>OH extract of <I>Brucea javanica</I>. All isolated compounds exhibited inactivation effects in systemic host plants, and compounds <B>3</B> and <B>4</B> were potent, with a minimum inhibitory concentration of 10 <I>μ</I>M. Furthermore, compound <B>3</B> was found to have a protective effect at the tested concentration of 40 <I>μ</I>M.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A green fluorescent protein tagged pepper mottle virus based antiviral assay can simply evaluate antiviral potential against PepMoV. </LI> <LI> PepMoV-GFP based leaf-disc method led to the isolation of five quassinoids from <I>Brucea javanica</I> with antiviral activity. </LI> <LI> All isolated compounds exhibited effective anti-PepMoV activity in systemic host plants. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>