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A Cross-Cultural Comparison of Fears in Turkish, South Korean, and American Students
Joy J. Burnham,Sunkyung Kim,Begum Serim Yildiz,Morgan Kiper Riechel,Ozgur Erdur-baker,Christina N. Kirby,Juliana K. Morgado 한국상담학회 2016 Journal of Asia Pacific counseling Vol.6 No.1
Fears of children from Turkey, South Korea, and the United States were compared for the first time using translated versions of the American Fear Survey Schedule (FSSC-AM; Burnham, 2005). Although several versions of the Fear Survey Schedule for Children (FSSC; Scherer & Nakamura, 1968) have been used since it was introduced, no published studies have reported the fears of South Korean children, and only a limited number of Turkish studies have used the FSSC (Serim, 2010; Serim-Yıldız & Erdur-Baker, 2013; Serim-Yıldız, Erdur-Baker, & Bugay, 2013). This exploratory study presents data from middle and high school students from two countries rarely studied in the fear literature. Fears of school-aged children in Asia and Eurasia are presented and compared to an American sample. The sample included 637 middle and high school students, and significant differences were found across age, gender, and country, and in most common fears. Implications for counseling and future research will be discussed.
Genetic diversity analysis of wild Codonopsis lanceolata in Korea using SSR makers
Serim Kim,Ji Hee Jeong,Jinsu Gil,Tae Dong Kim,Yurry Um,Ok Tae Kim,Ho Bang Kim,Yi Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
In this study, genetic diversity of wild Codonopsis lanceolata collected in Korea were analysed using SSR makers. Wild C. lanceolata roots were collected in Jeollanam-do Jangheung-gun Choentae Mountain as in roots. The wild C. lanceolata plants were cultivated in Chungbuk National University greenhouse and the leaves were sampled from 36 plants. The genomic DNA of C. lanceolata was extracted using CTAB. PCR was performed using a program of 35 cycles at 94℃ for 30 sec, 60℃ for 30 sec, and 72℃ for 30 sec with an pre-denaturation of 94℃ for 5 min and a final extension of 72℃ for 30 min. The PCR reaction mixture contains 5 pmole of primers and 20 ng of DNA template in a 20 μL reaction volume. The genotype of the analyzed samples were very different. Therefore, the wild C. lanceolata collected in Korea look genetically diverse.
Simple sequence repeat marker development from Codonopsis lanceolata and genetic relation analysis
Kim, Serim,Jeong, Ji Hee,Chung, Hee,Kim, Ji Hyeon,Gil, Jinsu,Yoo, Jemin,Um, Yurry,Kim, Ok Tae,Kim, Tae Dong,Kim, Yong-Yul,Lee, Dong Hoon,Kim, Ho Bang,Lee, Yi The Korean Society of Plant Biotechnology 2016 식물생명공학회지 Vol.43 No.2
In this study, we developed 15 novel polymorphic simple sequence repeat (SSR) markers by SSR-enriched genomic library construction from Codonopsis lanceolata. We obtained a total of 226 non-redundant contig sequences from the assembly process and designed primer sets. These markers were applied to 53 accessions representing the cultivated C. lanceolata in South Korea. Fifteen markers were sufficiently polymorphic, and were used to analyze the genetic relationships between the cultivated C. lanceolata. One hundred three alleles of the 15 SSR markers ranged from 3 to 19 alleles at each locus, with an average of 6.87. By cluster analysis, we detected clear genetic differences in most of the accessions, with genetic distance varying from 0.73 to 0.93. Phylogenic analysis indicated that the accessions that were collected from the same area were distributed evenly in the phylogenetic tree. These results indicate that there is no correlative genetic relationship between geographic areas. These markers will be useful in differentiating C. lanceolata genetic resources and in selecting suitable lines for a systemic breeding program.
Analysis of genetic diversity of Codonopsis lanceolata cultivated in Korea using SSR makers
Serim Kim,Ji Hee Jeong,Jinsu Gil,Tae Dong Kim,Yurry Um,Ok Tae Kim,Ho Bang Kim,Hee Chung,Yi Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Codonopsis lanceolata is a perennial climber. The roots are used as medicinal materials or vegetables. Recently, demand for C. lanceolata is increasing as a healthy food. C. lanceolata is distributed in India and East Asia such as China, Japan as well as Korea. In South Korea, this plant is widely cultivated in Gangwon-do province. No C. lanceolata varieties were developed in Korea. The objective of this study is to analyze genetic diversity of C. lanceolata cultivated in Korea using SSR makers. C. lanceolata roots were collected in each region were cultivated in Chungbuk National University greenhouse. Samples were obtained from fresh leaves of 5 plants from each collection region. The genomic DNA was extracted using CTAB. Genetic diversity was analysed using 4 sets of C. lanceolata SSR makers. PCR was performed in total 20 μL reaction volume containing 20 ng of DNA template, 5 pmole of primers. The genotypes of the analyzed samples were very similar. That means that the genetic diversity of C. lanceolata cultivated in Korea is very low.
Kim, Hyera,Cha, Seung-Ick,Shin, Kyung-Min,Lim, Jae-Kwang,Oh, Serim,Kim, Min Jung,Lee, Yong Dae,Kim, Miyoung,Lee, Jaehee,Kim, Chang-Ho The Korean Academy of Tuberculosis and Respiratory 2014 Tuberculosis and Respiratory Diseases Vol.77 No.3
Background: Bronchial anthracofibrosis (BAF), which is associated with exposure to biomass smoke in inefficiently ventilated indoor areas, can take the form of obstructive lung disease. Patients with BAF can mimic or present with an exacerbation of chronic obstructive pulmonary disease (COPD). The purpose of the current study was to investigate the prevalence of BAF in Korean patients with COPD exacerbation as well as to examine the clinical features of these patients in order to determine its clinical relevance. Methods: A total of 206 patients with COPD exacerbation were divided into BAF and non-BAF groups, according to computed tomography findings. We compared both clinical and radiologic variables between the two groups. Results: Patients with BAF (51 [25%]) were older, with a preponderance of nonsmoking women; moreover, they showed a more frequent association with exposure to wood smoke compared to those without BAF. However, no differences in the severity of illness and clinical course between the two groups were observed. Patients in the BAF group had less severe airflow obstruction, but more common and severe pulmonary hypertension signs than those in the non-BAF group. Conclusion: Compared with non-BAF COPD, BAF may be associated with milder airflow limitation and more frequent signs of pulmonary hypertension with a more severe grade in patients presenting with COPD exacerbation.