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Kim, Yong-Eun,Choi, Sunkyung,Kim, Jong ,Ok,Kim, Kee ,K. Portland Press Ltd. 2017 Bioscience reports Vol.37 No.1
<P>RBFOX3, a nuclear RNA-binding protein, is well known as a regulator of alternative pre-mRNA splicing during neuronal development. However, other functions of RBFOX3 are poorly understood. Here, we investigated the function of RBFOX3 in the cytoplasm with respect to regulation of Claudin-1 expression. In human lung tissue, Claudin-1 is higher in RBFOX3-positive cells than in RBFOX3-negative cells. Immunostaining and mRNA quantification revealed that protein levels, but not mRNA levels, of Claudin-1 are increased by RBFOX3. In addition, cycloheximide treatment of human lung cancer cells revealed that RBFOX3 increases the stability of Claudin-1 through attenuation of its ubiquitination. Our study provides insights into the molecular mechanisms by which RBFOX3 regulates Claudin-1 expression in human lung tissue.</P>
Kim Hyojin,Yoon Yune-Jung,Kim Hyunmi,Cha Eun-Young,Lee Hye Suk,Kim Jeong-Han,Yi Kyu Yang,Lee Sunkyung,Cheon Hyae Gyeong,Yoo Sung-Eun,Lee Sang-Seop,Shin Jae-Gook,Liu Kwang-Hyeon The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.11
KR-33028 (N-[4-cyano-benzo[b]thiophene-2-carbonyl]guanidine) is a new cardioprotective agent for preventing ischemia-reperfusion injury. This study was performed to identify the metabolic pathway of KR-33028 in human liver microsomes and to compare its metabolism with that of cryopreserved human hepatocytes. Human liver microsomal incubation of KR-33028 in the presence of NADPH and UDPGA resulted in the formation of four metabolites, M1, M2, M3, and M4. M1 and M2 were identified as 5-hydroxy-KR-33028 and 7-hydroxy-KR-33028, respectively, on the basis of LC/MS/MS analysis with the synthesized authentic standard. M3 and M4 were suggested to be dihydroxy-KR-33028 and hydroxy-KR-33028-glucuronide, respectively. Metabolism of KR-33028 in cryopreserved human hepatocytes resulted in the formation of M1, M2, and M4. These data show a good correlation between major metabolites formed in human liver microsomes and cryopreserved human hepatocytes. In addition, KR33028 was found to inhibit moderately the metabolism of CYP1A2 substrates. Based on the results obtained metabolic pathway of KR-33028 is proposed.
Tree Motifs in Seventh-century Silla Steles
KIM SUNKYUNG 계명대학교 한국학연구원 2018 Acta Koreana Vol.21 No.2
Stone steles served multiple purposes in different cultures: as a territorial marker, an edifying tablet, a political edict, a votive altar, a funerary monument, or a celebratory reminder of remarkable individuals or events. Chinese steles carved with images of Buddhist deities are monuments that testify to the process of adoption and adaptation across different cultural traditions. As products of the Buddhist appropriation of non-Buddhist Chinese steles, steles with Buddhist imagery are hybrids. The visual dialogue between two realms—the mortuary and the religious— underwent another twist when Buddhist steles first appeared on the Korean peninsula in the seventh century. The carvings on Korean steles displayed the usual prominent Buddhist deities and the formulaic language of a dedicatory inscription, but were made in the former territory of a defeated kingdom under a new administrative reign. Hence, they tell us about the fluctuating boundary between political entities, the social identity of the donors, and desired destinations of the devotees. Although “set in stone,” they never easily manifest a single fixed reading of the visual messages embedded in them. In order to better understand the paradoxically fluid character of unyielding stone, this article discusses some anomalous elements of these steles. Focusing on a few peculiar examples of steles from 6th century China and 7th century Korea, this article explores the roles of subsidiary motifs, such as trees and pavilions, found across geographic/cultural borders.
Kim, Sunkyung,Ko, Kyoung Chul,Lee, Jin Yong,Illas, Francesc The Royal Society of Chemistry 2016 Physical chemistry chemical physics Vol.18 No.34
<P>Titanium dioxide (TiO2), as a semiconductor metal oxide, has been one of the most popular materials studied in the field of photocatalysis. In the present study, the properties of single oxygen vacancies of (TiO2)(35), a prototype of an anatase nanoparticle, were investigated by DFT calculations. (TiO2)(35) is the minimum sized model (similar to 2 nm) for a bipyramidal nanoparticle with anatase phase and eight {101} facets. All the available oxygen vacancies at various sites according to position, coordination number, and distance from the center atom were examined. The geometric, energetic and electronic properties of the reduced TiO2 clusters were analyzed by hybrid DFT functionals with different Hartree-Fock exchange ratios (0, 12.5 and 25%). It was found that the structure of pristine (TiO2)(35) is somewhat different from the bulk lattice, with a relatively high surface to volume ratio. Moreover, the particular highly (three)-coordinated oxygen atom is energetically the most favorable for oxygen vacancy formation from the nanoparticle mainly due to its substantially high relaxation energy. TiO2 nanoparticles have low oxygen vacancy formation energy and narrow band gap because of their defect states, and can be utilized as an efficient photocatalyst material.</P>
Kim, Mi Jeong,Moon, Chang-Hyun,Kim, Mi-Young,Lee, Sunkyung,Yi, Kyu Yang,Yoo, Sung Eun,Lee, Soo Hwan,Baik, Eun Joo,Jung, Yi-Sook Elsevier 2005 european journal of pharmacology Vol.525 No.1
<P><B>Abstract</B></P><P>A novel Na<SUP>+</SUP>/H<SUP>+</SUP> exchanger-1 (NHE-1) inhibitor [5-(2-methoxy-5-chloro-5-phenyl)furan-2-ylcarbonyl]guanidine (KR-32570) has been previously demonstrated to elicit cardioprotective effect against ischemic injury in rat heart. In the present study, we examined the effects of KR-32570 on cell death induced by hypoxic insult in heart-derived H9c2 cells. Treatment with KR-32570 (1–10 μM) significantly reduced hypoxia-induced necrotic cell death (lactate dehydrogenase release) and apoptotic cell death (TUNEL-positivity, caspase-3 activity). KR-32570 also decreased the cytosolic and mitochondrial Ca<SUP>2+</SUP> overload induced by hypoxia. Inhibition of mitochondrial Ca<SUP>2+</SUP> overload by ruthenium red mimicked the anti-apoptotic effect of KR-32570. In addition, KR-32570 significantly recovered the large reduction in mitochondrial membrane potential (Δ<I>Ψ</I><SUB>m</SUB>) and cytochrome <I>c</I> release induced by hypoxia. Taken together, our results suggest that a new NHE-1 inhibitor KR-32570 elicits potent cardioprotective effects in H9c2 cells, and its effects may be mediated by inhibition of intracellular Ca<SUP>2+</SUP> overload and mitochondrial death pathway during hypoxia.</P>