Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

구리 지역에서 조사한 고혈압의 인지도, 치료률 및 관리률

홍철호,김순길,홍택원,이제,신진호,이재웅,김경수,김정현,임헌길,이방헌 대한간질학회 2003 대한간질학회지 Vol.9 No.1

Multiple Markers of Contrast Induced Nephropathy after the Percutaneous Coronary Intervention

Byoung-won Park,Seong Soon Kwon,Min Ho Lee,Do Hoi Kim,Min Su Hyon,Duk Won Bang 순천향대학교 순천향의학연구소 2018 Journal of Soonchunhyang Medical Science Vol.24 No.1

Objective: Contrast-induced nephropathy (CIN) frequently occurs after percutaneous intervention. Objective of this study was to investigate the usefulness of serum cystatin C, neutrophil gelatinase-associated lipocalcin (NGAL), urinary kidney injury molecule-1 (KIM-1), and interleukin-18 (IL-18) as early predictors for CIN after percutaneous coronary intervention (PCI). Methods: In 53 patients who underwent PCI were enrolled. Serum creatinine and cystatin C level were measured immediately before, and 24 hours and 48 hours after catheterization. Serum NGAL, urinary KIM-1, and IL-18 were measured immediately before, and 4 hours, 24 hours, and 48 hours after catheterization. CIN was defined as a rise in creatinine 0.5 mg/dL or 25% above baseline. Results: CIN occurred in four patients (7.5%). Serum cystatin C levels were higher at 24 hours and 48 hours in CIN patients than in those without CIN (P<0.05). Serum NGAL levels were higher at 48 hours in CIN patients than in those without CIN. Urinary KIM-1 levels were higher at 48 hours in CIN patients than in those without CIN. There were no significant markers of CIN on multi-variate analysis. Conclusion: In this study, the occurrence of CIN after PCI was 7.5%. Although there were some time-course changes in serum cystatin C and urinary KIM-1 after PCI, there was no significant predictor for CIN after PCI.

Retinoic Acid가 흰쥐 태자 혀조직의 분화와 Laminin 합성에 미치는 영향에 대한 연구

김혜주,정호삼,서윤경,백두진,김원규,윤지희,이방헌 대한췌담도연구회 2000 대한췌담도연구회지 Vol.5 No.4

상부요로의 이행상피세포암

김봉주,김호성,류수방 全南大醫大 1987 전남의대학술지 Vol.24 No.3

인터넷 마케팅의 성과지표에 관한 통합적 평가모형

방호열,김민숙 국제무역학회 2003 국제무역연구 Vol.9 No.2

본 논문은 인터넷 마케팅의 성과를 측정할 수 있는 통합적 평가모형을 제시하는데 그 목적이 있다. 이를 위해 인터넷 마케팅의 효율성을 고려한 전환효율성 모형과 기존의 마케팅 문헌에서 중요시하고 있는 재무적 성과와 고객차원의 효과성을 통합함으로써 다차원적인 성과평가모형을 개발하였다. 이러한 연구의 필요성을 뒷받침하는 근거로는 다음의 세 가지를 들 수 있다. 첫째, 인터넷 마케팅의 성과지표로서 웹사이트의 효율성을 분석하는 모형은 웹사이트에 접속하여 구매하는 고객 수만을 고려한 양적인 측정이라는 한계가 있다. 둘째, 마케팅의 효율성에 중점을 두는 재무적 차원의 성과지표는 단기적 관점을 중시하는 한계가 있어, 고객차원의 효과성과 같은 장기적 목표가 그 대가로 희생될 가능성이 있다. 셋째, 기존의 마케팅 문헌에서 제시하는 다차원적인 성과지표는 이들 지표가 가지는 암묵성과 복잡성으로 실무경영자가 실제로 적용하는데 있어서 현실적 어려움이 있다. 이상의 한계를 극복하기 위해 본 논문에서 제시한 통합적 성과모형은 다음과 같은 세 가지 차원의 상호관련성을 체계적으로 통합한 모형이다. 첫째, 시간차원을 도입하여 인터넷 마케팅의 효율성을 평가하는 단기적 지표, 재무적성과를 측정하는 단기 및 중기적 지표, 고객차원의 효과성을 평가하는 중기 및 장기적 목표들간의 시간상의 우선 순위를 체계화하였다. 둘째, 같은 시간차원에 있는 성과지표들간에 있어서도 성과의 발생과정에 따라 우선순위를 파악하고, 이들간의 상호관계를 분석할 수 있게 하였다. 셋째, 세 가지의 성과지표들을 인과적으로 연결짓기 위해 선순환적인 강화사이클(reenforcing cycle)로 통합시키고 있다. 이러한 성과강화사이클은 단기적 성과지표, 중기적 성과지표, 장기적 성과지표들 내부에서 독립적으로 이루어지는 미시적인 강화사이클과 이들 전체를 통합하는 거시적인 강화사이클로 구분된다. This studies' purpose is mainly concerned about integrating internet marketing performance measures. The efficiency of Internet marketing has been perceived in the only number of customers in the present academics. Therefore, we must consider the quality-approach regarding the financial performance measure in the short run, and simultaneously, non-financial performance measure based on the customer-based approach also must be needed in the long run. First of all, we explore the main characteristics of internet marketing and the differences between marketing communications in the traditional media and new digital medias. The characteristics of conversion marketing plays an important role in internet marketing and then world wide web as a marketing communication tool is the most strong marketing medium to move the customer front being a passive internet surfer to an interactive user of a medium, and purchaser of products or services, a process of conversion efficiency. Second, we review the history and interrelationships of marketing performance measures and examine important measures in the mutidimensional measures of performance. Figuring out which of many measures is necessary for conscientious manager and we are able to reduce these measure to a more manageable set. Finally, we integrate the measures of internet marketing as channel operational dimension, the measures from financial dimension, and the measures of customer-based dimension. Accordingly, strong internet brand delivers success to the company in the long run.

주행차량에 의한 강교량의 충격계수에 관한 연구

김상효,이상호,김방래 연세대학교 산업기술연구소 1996 논문집 Vol.28 No.1

In this study, a finite element program which can perform the dynamic analyses of bridges by considering the interaction forces between the bridge and moving vehicle is developed. The impact factors and dynamic behaviors of bridges according to the various parameters, such as speed and weight of vehicles, span length, damping ratio, girder spacing and transverse stiffnesses of bridges, are studied. The impact factor, in general, increases as the traveling speed increases, whereas the weight of vehicle does not affect the impact factor. The impact factor is larger in exterior girders than in interior girders. As the spacing between two girders increases, the impact factor decreases, and it is also affected by the lateral rigidities of the bridges. In addition, the impact factor is smaller in continuous bridges than in simply supported bridges.

혈부축어탕의 고지혈증 유발 생쥐 신장내 지방 축적 감소효과

김호현,강윤호,방혜정,김진택,박인식,안상현,이해풍 世明대학교 한의학연구소 1998 韓醫學硏究所 論文集 Vol.1 No.-

Renal tissues of ICR mouse were intraperitoneally injected with Hyulboochucketang(HCE;1.28g/kg/day) after Triton WR-1339(TX;600mg/kg) injection were observed to investigate the lipid accumulation suppressive effect of HCE. The renal tissues were obtained at hour 24,48 and 72 after TX injection that were fixed in fromol-calcium solution and were cryocut. The tissue stained by H&E for general morphology, sudan black B for lipid and perchloric acid-naphthoquinone method for cholesterol. The parietal layer of Bowman's capsule is swelled as cuboidal type at hour 48 after TX injection and the lipid blot and cholesterol particle were noticeably increased in glomerulus than control group. But the parietal layer of Bowman's capsule in HCE treated group was shaped as squamous type that was looked like as normal morphology and the lipid blot and cholesterol particle w3ere considerably decreased in glomerulus than TX group. As results indicated that the accumulation of lipid including cholesterol caused by TX injection were mitigated in renal tissues by the antihyperlipidermic effect of HCE.

Bacillus subtilis의 cdd유전자의 Escherichia-Saccharomyces Shuttle Vector YRp7에의 클로닝

金榮基,宋邦鎬 경북대학교 과학교육연구소 1988 科學敎育硏究誌 Vol.12 No.-

The cdd gene enconding cytidine deaminase from Bacillus subtilis was cloned and expressed in the Saccharomyces cerevisiae D13-1A. By insertion of the 1.45 Kb of EcoRl/EcoRl fragment which containing genes fo for replication origin and trpl selective marker from Escherichia-Sac-charomyces shuttle vector YR_p7 into the EcoRl site of pS062, the Bacillus cdd gene was expressed in the yeast cells. By selection of the trp+ colonies in the SD medium. One of the transformed Saccharomyces cells were isolated as Saccharomyces cerevisiae YKl. The transformed yeast cells which carring chimeric plasmid, pYKl, was expressed the cdd activity with two folds elevated levels. For further confirming the pYKl expression in yeast cell, the pYKl which isolated from the transformed cells was recloned into the Escherichia coli KM268. The plasmid isolated from strain KM268 was identified as the same one with the original chimeric plasmid, pYKI.

E-business가 지식경영활동에 미치는 영향

방호열,김성호 국제무역학회 2002 국제무역연구 Vol.8 No.1

E-business가 지식경영에 미치는 영향을 파악하고 하는 것이 본 연구의 목적이다. 이를 위해서는 E-business를 세 가지의 차원(E-business 발전단계, E-business 전략, E-business 정보시스템)으로 분류를 하였고, 지식경영활동은 지식이전·지식활용의 차원으로 분류를 하여 분석을 실시하였다. 연구의 모형은 E-business가 지식이전에 영향을 미치고 지식 이전은 지식활용에 영향을 미친다라고 설정을 하였다. 제시된 가설을 분석한 결과는 다음과 같다. E-business의 발전단계와 정보시스템은 지식이전에 부분적으로 영향을 미치고 지식이전은 지식활용에 있어서 부분적으로 영향을 미치는 것으로 나타났다. The purpose of this study is that E-business affects knowledge management. To achieve E-business suggests three dimensions(E-business development stages, E-business strategy, E-business information system) and knowledge management activities do knowledge transfer application. The research model of this paper is that E-business affects knowledge transfer, knowledge transfer affects knowledge application. To test model survey research is conducted. Data gathered from Korea Manufacturing firms that adopted E-business. The empirical results show that E-business development stages and information system partially affect knowledge transfer, knowledge transfer also partially affects knowledge application.