MPP^+와 6-OHDA에 대한 한약탕제의 보호효과 연구

강봉주,홍성길,조동욱 한국한의학연구원 1999 한국한의학연구원논문집 Vol.5 No.1

The effect of herbal medicine on 1-methyl-4-phenylpyridinium ion(MPP+) and 6-hydroxydopamine(6-OHDA) mediated neurotoxicity was studied in the rat phaeochromocytoma cell line PC12. The present study was designed to test the hypothesis that herbal medicine can protect cells from neurotoxiciy caused by MPP+ and 6-OHDA. Exposure of PC12 cells to 0.2mM MPP+ and 50 μM 6-OHDA for 24h resulted in a 50% cell death with respect to the control cells. MPP+ induced cell death was reduced by Yollyounggobondan(延齡固本丹), Sagunjatang(四君子湯), Palmihwan(八味丸), and Palmultang(八物湯) (p<0.05). However, herbal medicines did not protect cells trom degeneration caused by the 6-OHDA. Yollyounggobondan, Yungmijihwangwon(六味地黃元), Palmultang, and Samultnag(四物湯) were effective in protecting against MPP+ induced ATP loss in PC12 cells (p<0.05). Yollyounggobondan and Palmultang were effect in neurite protection against 6-OHDA treatment in differentiated PC12 cells with NGF.

활성형 Vitamin D₃ 전환능을 소유한 방선균에서 P-450 Hydroxylase유전자의 클로닝 전략

서주원,현창구,김정미,김승영,홍순광 명지대학교 자연과학연구소 1998 자연과학논문집 Vol.17 No.-

비타민 D₃는 포유동물이나 새의 간에서 25-hydroxyvitamin D₃[25(OH)D₃]로 전환되는데, 이 hydroxylation은 P-450 hydroxylase에 의해 촉매된다고 알려져있다. 본 연구에서는 활성형 비타민 D₃전환능을 소유한 방선균에서P-450 hydroxylase효소를 신속하게 분리가능한 PCR방법을 보고하고자 한다. Primer는 방선균에서 보고된 P-450 hydroxylase들 사이의 아미노산 서열의비교를 통해 산소결합부위와 heme-ligand pocket 주의에서 제작되었고,5종의 방선균에서 효과적으로 PCR증폭산물을 획득할 수 있었다. 증폭산물을 획득할 수 있었다.증폭 산물들의 아미노산서열을 분석한 결과 기존에 밝혀진 많은P-450 hydroxylase유전자들과 상동성을 보이고 있었으며, 특히 Streptomyces griseolus에서 분리되어 sulfonylurea 등의 제초제의 분해능력을 가진 SuaC, SubC 효소 등과 높은 아미노산 상동성을 지니고 있었다. 이러한 PCR전략은 방선균에서 유용한 물질의 대사에 관여하는 P-450 hydroxylase효소의 클로닝에 도움을 줄 것이라 사료된다 In mammals and birds, vitamin D₃is converted to 25-hydroxyvitamin D₃[25(OH)D₃] and then to 1α25-dihydroxyvitamin D₃[25(OH)₂D₃]in the liver and kideny, respectively these hydroxylating reactions are known to be catalyzed by P-450 hydroxylases. In this paper we report a PCR method which can be used for the repid amplification of DNA fragments for _450 hydroxylase from actinomycetes which can convert to active from of vitamin D₃. Primers were designed based on several regions sharing strong similarities in amino acid sequence of P-450 hydroxylases from a variety of actinomycetes, primarily in the regions of an oxygen binding site and a heme ligand pocket. These primers were used to amplify DNA fragments from five different actinomycetes. The deduced amino acid sequences of the isolated fragments revealed significant similarities to known P-450 hydroxylase including the product of the suaC or subC genes from Streptomyces griseolus that is capable of metabolizing a number of sulfonylurea herbicides and to the product of the P450sca2 from S. carbophilus that produces a specific HMG-CoA reductase inhibitor. This method will be helpful for the researchers in cloning the genes for P-450 hydroxylase involved in the biosynthesis of useful compounds.

렙토스피라병 초기의 산 염기 평형 변화와 흉부 X - 선 소견

김홍주(Hong Joo Kim),정자현(Ja Hun Jung),이재웅(Jae Ung Lee),이오영(Oh Young Lee),양석철(Suck Chul Yang),한동수(Dong Soo Han),손주현(Joo Hyun Sohn),김순길(Soon Kil Kim),김호중(Ho Jung Kim),박성광(Seoung Kwang Park),강성귀(Seoung Gwi 대한내과학회 1997 대한내과학회지 Vol.52 No.1

N/A Background: Clinical syndrome of leptospirosis in Korea which accompanied by hemoptysis, pulmonary hemorrhage, respiratory failure was quite different from that of typical Weil's disease. The death rate in the early stage of disease has relationship with chest X-ray findings and acid-base disturbances resulted from hypoxia and asphyxia. We've come to consider chest X-ray findings and arterial blood gas findings as factors influencing the prognosis of the disease. Methods: Forty one cases of leptospirosis diagnosed by acute febrille illness symptoms and serologic tests were graded for chest X-ray findings and grouped according to acid-base abnormalities. Retrospectively, we attempted to correlate the acid- base disturbances and chest X-ray findings with morbidity and mortality of the disease. Results: The result were as follows. 1) Abnormal chest X-ray findings were observed in 30 cases(73%). Mortalities according to the chest X-ray findings were 0(0%), 2(17%), 3(0%), 4(0%), 5(33%), 6(50%) and chest X-ray findings influenced the mortality with statistical significance(p<0.05). 2) Acid-base abnormalities were observed in 39 cases(95%) and the types were divided to six groups . Respiratory alkalosis was the most common acid-base disturbance(44%). Five cases were died. Two of five were respiratory alkalosis, two cases were mixed respiratory metabolic acidosis and another case was mixed respiratory-metabolic alkalosis. As shown, acid-base abnormality influenced the mortality and mixed acid-base disturbances were showed high mortality with statistical significance (p<0.05). 3) Total mortality was 12%(five of forty-one). The chest X-ray score, morbidity, pH, pCO2, pO2, and HCO3 were 5.0±0.8, 3.4±1,3, 7.16±0.17, 32.5±1.1, 43.3±13.4 and 17.6±3.1 in died group, 2.4±0.4, 12.7±1.0, 7.46±0.01, 48.8±13.4, 65.4±3.7 and 23.4±0.9 in recovery group, respectively. There was difference between two groups with statistical significance. Conclusion: In this study, we concluded that chest X-ray findings and acid-base abnormalities influenced the mortality of leptospirosis. The factors above mentioned make us consider chest X-ray and blood gas analysis are essential to the patient hospitalized for the suspicion of leptospirosis. Especially, these factors pointed out the patient having chest X-ray abnormalities and mixed acid-base disturbances needed intensive care in the early stage of leptospirosis.

Tensile properties and stress whitening of polypropylene/polyolefin elastomer/magnesium hydroxide flame retardant composites for cable insulating application

Hong, Chae Hwan,Lee, Yong Bum,Bae, Jin Woo,Jho, Jae Young,Uk Nam, Byeong,Chang, Do-Hoon,Yoon, Seoung-Hoon,Lee, Kun-Joo Wiley Subscription Services, Inc., A Wiley Company 2005 Journal of applied polymer science Vol.97 No.6

<P>Flame retardant polypropylene (PP) composites were prepared by combining random polypropylene with uncoated and surface-treated forms of magnesium hydroxide filler and elastomeric modifiers, with and without maleic anhydride functionalization. Four types of magnesium hydroxide (MDH) with different surface treatments were compounded at amounts up to 60% by weight to PP/polyolefin elastomer (POE) matrix resin to obtain a series of composites. The tensile strength and elongation at break were measured. MDH coated with polymeric material was found to give a high elongation at break value compared with the values obtained with uncoated and vinyl silane and amino silane coated MDH. Two types of POE, i.e., neat and maleic anhydride grafted POE (POEgMA), were used to investigate the stress whitening of composites in bending deformation. POEgMA used composites showed no stress whitening while neat POE used composites showed whitening when bended. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 97: 2311–2318, 2005</P>

Outcomes of Robotic Living Donor Right Hepatectomy from 52 Consecutive Cases: Comparison with Open and Laparoscopy-Assisted Donor Hepatectomy

( Seoung Yoon Rho ),( Dai Hoon Han ),( Jae Geun Lee ),( Dong Jin Joo ),( Myung Soo Kim ),( Soon Il Kim ),( Jin Sub Choi ),( Gi Hong Choi ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: To investigate the feasibility and safety of an alternative robotic living-donor right hepatectomy (RLDRH) technique.Data for minimally invasive living-donor right hepatectomy, especially RLDRH, in a relatively large donor cohort have not been reported yet. Methods: From March 2016 to March 2019, 52 liver donors underwent RLDRH. The clinical and perioperative outcomes of RLDRH were compared with those of conventional open donor right hepatectomy (CODRH; n=62) and laparoscopy-assisted donor right hepatectomy (LADRH; n=118). Donor satisfaction with cosmetic results was compared between RLDRH and LADRH using a body image questionnaire. Results: Although RLDRH had a longer operative time (RLDRH, 493.6 min; CODRH, 404.4 min; LADRH, 355.9 min, P<0.001), its mean estimated blood loss was significantly lower (RLDRH, 109.8 mL; CODRH, 287.1 mL; LADRH, 265.5 mL; P<0.001). The postoperative complication rates were similar among the three groups (RLDRH, 23.1%; CODRH, 35.5%; LADRH, 28.0%; P=0.420). Regarding donor satisfaction, the body image and cosmetic appearance scores were significantly higher in RLDRH than in LADRH. There was no significant difference in hospital stay among the three groups (P=0.105). After propensity score matching, RLDRH showed a shorter hospital stay and similar complication rate than CODRH. Conclusions: RLDRH resulted in a similar postoperative complication rate and shorter length of hospital stay compared with those of CODRH and provided better body image and cosmetic results compared with those of LADRH. RLDRH is feasible and can be safely performed by expert surgeons in both robotic systems and open hepatectomy.

Vista Hall : HER-2/neu in Human Extrahepatic Cholangiocarcinoma

( Hong Joo Kim ),( Tae Woo Yoo ),( Dong Il Park ),( Jung Ho Park ),( Yong Kyun Cho ),( Chong Il Sohn ),( Woo Kyu Jeon ),( Byung Ik Kim ),( Min Kyung Kim ),( Seoung Wan Chae ),( Jin Hee Sohn ) 대한소화기학회 2006 대한소화기학회 추계학술대회 Vol.2006 No.-

Plasma markers of oxidative stress, inflammation and endothelial cell injury in diabetic patients with overt nephropathy administered alpha-lipoic acid and angiotensin II receptor blocker

Hong, Seong-Bin,Uhm, Wook-Hyun,Joo, Woo-Chul,Nam, Moon-Suk,Lee, Seoung-Woo,Song, Joon-Ho The Korean Society of Toxicogenomics and Toxicopro 2010 Molecular & cellular toxicology Vol.6 No.2

Alpha-lipoic acid (ALA) is a natural antioxidant that scavenges reactive oxygen species (ROS) and regenerates endogenous antioxidants. In the present study, plasma molecular markers of oxidative stress, inflammatory state, and endothelial cell injury was measured to investigate the effect of simultaneous blocking of both angiotensin (AT) II-and oxidative stress-mediated pathophysiologic pathways during the course of type II diabetic nephropathy in the clinical settings. Sixty-three diabetic subjects have carried out either of 12-month treatment course of AT-II receptor blocker (ARB) alone, ALA alone, or combination of both. Plasma malondialdehyde (MDA) level, a marker for oxidative stress, increased in ARB alone group whereas reduced to significantly lower level in ALA+ARB combination group. Plasma hs-CRP level, an inflammatory marker, decreased significantly in ARB+ALA group. Plasma thrombomodulin (TM) level, a marker of endothelial cell injury, increased in both ARB and ALA group. Twenty-four hour urinary protein excretion, as a marker of renal injury, decreased in ARB and ARB+ALA group. Finally, renal function was reserved well in ARB+ALA group in compared to others. The combined administration of ALA and ARB additively attenuated or reduced plasma markers of oxidative stress, inflammation and endothelial cell injury for one year. It suggests that the benefits in attenuating atherogenic process and vascular injury retard the progression of renal dysfunction.

현역 군인에게 발생한 동양안충 감염 1예

박주현(Joo Hyun Park),문찬식(Chan Shik Moon),홍승우(Seung Woo Hong),임성규(Seoung Kyu Im),이상협(Sang Hyup Lee),강신희(Shin Hee Kang) 대한검안학회 2009 Annals of optometry and contact lens Vol.8 No.1

목적: 군복무 중인 젊은 성인남자에서 동양안충의 감염을 경험하여 보고하고자 한다. 대상과 방법: 약 1주 전 시작된 좌안의 충혈과 자극증상을 보이던 21세 남자환자가 눈을 비비던 중 흰색의 선충을 발견하여 국군수도병원 안과외래에 내원하여 안과적 검사를 시행하였다. 결과: 검사 결과 결막충혈과 유두비대증상을 보였으며 총 2개체의 기생충이 발견되었고 혈액검사 및 안와, 두부자기공명영상 촬영 결과 이상소견은 없었으며 충체를 동정한 결과 동양안충 감염으로 확진 되었다. 결론: 동양안충의 인체 감염은 국내에서도 약 30예가 보고되어 있으나 임상적으로 드문 질환으로서 현역군인에서의 감염사례를 경험하여 보고하는 바이다. Purpose: To report the case with Thelazia callipaeda infection in young Korean male. Methods: A 21-year-old male patient visited Korean Armed Forces Capital hospital with chief complaints of conjunctival irritating symptoms and white colored tape warms in his left conjunctival sac. Results: The image study, hematologic and serologic test was within normal limit. The parasite was identified as Thelazia callipaeda. Conclusions: The Thelazia callipaeda infection of human eye is not common disease. We experience one case to report.

Alloxan 당뇨병에 대한 인삼의 효과

홍성표(Seoung-Pyo Hong),임무현(Moo-Hyun Yim),주현규(Hyun-Kyu Joo) 한국생약학회 1976 생약학회지 Vol.7 No.2

This study was aimed to investigate the effects of Ginseng ethanol extract on Diabetes induced by alloxan in comparison with that of insulin. The blood sugar, blood urea nitrogen, glutamic oxaloacetic transaminase and cholesterol were measured by spectrophotometry. The results were obtained as follows. 1. Ethanol extract of Ginseng increased the blood sugar level in normal rats. 2. Ethanol extract of Ginseng restrained the increase of blood sugar in diabetes induced by alloxan. 3. Ethanol extract of Ginseng acted to oppose a rapid decrease of blood sugar by insulin. 4. Blood urea nitrogen, glutamic oxaloacetic transaminase and cholesterol level in blood were not affected by Ginseng.

인삼이 흰쥐의 체중에 미치는 영향

홍성표(Seoung-Pyo Hong),임무현(Moo-Hyun Yim),주현규(Hyun-Kyu Joo) 한국생약학회 1976 생약학회지 Vol.7 No.2

The effect of alcohol extract of Ginseng orally administered on the changes of the body weight of rats was investigated. The body weight of rats during feeding with 0.1% Ginseng extract added in the diet was measured for 2 months and compared with that of normal rats. The result obtained in the experiment indicated that the change of body weight of rats (140∼170g) was not affected by Ginseng.