원발성 피부 CD30 양성 대형세포 림프종

양성규 ( Seong Gyu Yang ),조광현 ( Kwang Hyun Cho ),김방순 ( Bang Soon Kim ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.2

Primary cutaneous CD30(Ki- 1) positive large cell lymphoma occurs generally in adults and in contrast to primary noncutaneous CD30 positive large cell lymphoma, rarely in children and adolescences. Generally it does not express epithelial membrane antigen(EMA). Recently we experienced a sixteen-year old boy who developed CD30 positive large cell lymphoma. He presented with single ulcerative nodule on his right upper vermilion border and there was no evidence of lymph nodal or other visceral involvement. In paraffin section, stainings for CD30, CD45RO, CD45 and EMA were positive but staining for CD20 was negative. His young age of onset and positive immunoreactivity to EMA of tumor cells in this case are unusual features for primary cutaneous CD30 positive large cell lymphoma. (Kor J Dermatol 1996;34(2): 331-335)

Assessment of the efficacy of an attenuated live marker classical swine fever vaccine (Flc-LOM-BE^rns) in pregnant sows

Lim, Seong-in,Choe, SeEun,Kim, Ki-Sun,Jeoung, Hye-Young,Cha, Ra Mi,Park, Gil-Soon,Shin, Jihye,Park, Gyu-Nam,Cho, In-Soo,Song, Jae-Young,Hyun, Bang-Hun,Park, Bong-Kyun,An, Dong-Jun Elsevier Ltd. 2019 Vaccine Vol.37 No.27

<P><B>Abstract</B></P> <P>Here, we constructed an attenuated live marker classical swine fever (CSF) vaccine (Flc-LOM-BE<SUP>rns</SUP>) to eradicate CSF. This was done by taking infectious clone Flc-LOM, which is based on an attenuated live CSF vaccine virus (LOM strain), and removing the full-length classical swine fever virus (CSFV) E<SUP>rns</SUP> sequences and the 3′ end (52 base pairs) of the CSFV capsid. These regions were substituted with the full-length bovine viral diarrhoea virus (BVDV) E<SUP>rns</SUP> gene sequence and the 3′ end (52 base pairs) of the BVDV capsid gene. Sows were vaccinated with the Flc-LOM-BE<SUP>rns</SUP> vaccine 3 weeks before insemination and then challenged with virulent CSFV at the early, mid- or late stages of pregnancy. We then examined transplacental transmission to the foetuses. Piglets born to sows vaccinated with Flc-LOM-BE<SUP>rns</SUP> did not show vertical infection, regardless of challenge time. In addition, CSFV challenge did not affect the delivery date, weight or length of the foetus. Pregnant sows inoculated with the Flc-LOM-BE<SUP>rns</SUP> vaccine were anti-CSF E<SUP>rns</SUP> antibody-negative and anti-BVDV E<SUP>rns</SUP> antibody-positive. Challenge of pregnant sows with virulent CSFV resulted in anti-CSF E<SUP>rns</SUP> antibody positivity. These results strongly indicate that differential diagnosis can be conducted between the Flc-LOM-BE<SUP>rns</SUP> vaccinated animal and virulent CSFV affected animal by detecting antibody against BVDV E<SUP>rns</SUP> or CSF E<SUP>rns</SUP> gene. Therefore, the Flc-LOM-BE<SUP>rns</SUP> vaccine may fulfil the function of differential diagnosis which required for DIVA vaccine.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The Flc-LOM-BE<SUP>rns</SUP> is live attenuated DIVA vaccine for CSFV. </LI> <LI> The Flc-LOM-BE<SUP>rns</SUP> vaccine protected foetuses from vertical transmission. </LI> <LI> The Flc-LOM-BE<SUP>rns</SUP> vaccine enables differential identification of serum antibodies. </LI> </UL> </P>

T_H2 and T_H1 lung inflammation induced by airway allergen sensitization with low and high doses of double-stranded RNA

Jeon, Seong Gyu,Oh, Sun-Young,Park, Hye-Kyung,Kim, You-Sun,Shim, Eun-Jin,Lee, Hyun-Seung,Oh, Min-Hee,Bang, Boram,Chun, Eun-Young,Kim, Sang-Heon,Gho, Yong Song,Zhu, Zhou,Kim, You-Young,Kim, Yoon-Keun Elsevier 2007 The Journal of allergy and clinical immunology Vol.120 No.4

<P><B>Background</B></P><P>Although respiratory viral infections in early childhood can enhance the development of airway allergen sensitization, the exact mechanisms of the effects of viral infections on the adaptive immune response to inhaled allergens are controversial.</P><P><B>Objective</B></P><P>We sought to evaluate the effects of double-stranded RNA (dsRNA) on airway sensitization to inhaled allergens.</P><P><B>Methods</B></P><P>Novel mouse models were created through simultaneous airway sensitization to an allergen and low or high doses of dsRNA. The mouse models were applied to Toll-like receptor 3–, IL-13–, IL-4–, signal transducer and activator of transcription (STAT) 6–, IFN-γ–, and T-box expressed in T cells (T-bet)–deficient mice to evaluate underlying pathophysiologic mechanisms in the development of allergic lung inflammation.</P><P><B>Results</B></P><P>We found that airway allergen sensitization with dsRNA induced lung inflammation that was not present in Toll-like receptor 3–deficient mice. Moreover, lung inflammation enhanced by low-dose dsRNA was impaired in IL-13–deficient mice, whereas lung inflammation by high-dose dsRNA was impaired in IFN-γ–deficient mice. The models also demonstrated that low-dose dsRNA enhanced IL-4 expression during allergen sensitization and that inflammation enhanced by low-dose dsRNA was not present in IL-4– or STAT6-deficient mice. In contrast, the present study showed that high-dose dsRNA enhanced IFN-γ expression during allergen sensitization and that the development of lung inflammation enhanced by high-dose dsRNA was impaired in T-bet–deficient mice.</P><P><B>Conclusion</B></P><P>These findings suggest that airway allergen exposure during respiratory viral infections might induce asthma induced by both T<SUB>H</SUB>1 and T<SUB>H</SUB>2 immune responses to inhaled allergens.</P><P><B>Clinical implications</B></P><P>Targeting both T<SUB>H</SUB>1 and T<SUB>H</SUB>2 lung inflammation might be important in the treatment of virus-associated asthma.</P>

Intense Pulsed Light의 유래, 작용 원리와 임상 진료에서의 올바른 사용

조성문 ( Seong Moon Jo ),김방순 ( Bang Soon Kim ),김혜성 ( Hei Sung Kim ),고주연 ( Joo Yeon Ko ),노미령 ( Mi Ryung Roh ),양성규 ( Seong Gyu Yang ),유화정 ( Hwa Jung Ryu ),최재우 ( Jae Woo Choi ),황지환 ( Ji Hwan Hwang ),박현선 ( H 대한피부과학회 2013 大韓皮膚科學會誌 Vol.51 No.11

Intense pulsed light (IPL) is a high-intensity polychromatic incoherent light of a determined wavelength spectrum, fluence, and pulse duration. Clinicians can combine these variable factors of IPL for the purpose of selective destruction of target chromophores. Due to these properties, IPL has been widely used for numerous indications including hair removal and treatment of vascular or pigmented lesions. For skilled and experienced clinicians, IPL is a great treatment modality; however it can be a source of serious adverse effects when performed by untrained clinicians. Thus, to promote the proper and safe use of IPL, we summarized the origin, history, basic principles, and clinical application of IPL and discussed qualification factors needed for clinicians who use IPL. (Korean J Dermatol 2013;51(11):845∼850)

시프로플록사신에 의해 발생한 지연형 아나필락시스

강민규 ( Min Gyu Kang ),김주영 ( Ju Young Kim ),방보람 ( Bo Ram Bang ),정재우 ( Jae Woo Jung ),손성욱 ( Seong Wook Sohn ),강혜련 ( Hye Ryun Kang ) 대한천식알레르기학회 2012 천식 및 알레르기 Vol.32 No.3

Background: Ciprofloxacin can induce immediate hypersensitivity reactions such as urticaria, angioedema, and anaphylaxis. These reactions are usually immunoglobulin E -mediated but non-immunoglobulin E mediated reactions were also reported. Case History: A 47-year-old man visited our emergency department with generalized erythematous rasheses. Skin rash, febrile sensations and conjunctival injection had developed 1 hour after he took ciprofloxacin and tamsulosin 5 hours ago. Initially, his blood pressure was normal but abruptly dropped to 75/36 mmHg 5 hours after arrival. His blood pressure was normalized with repeated epinephrine injection followed by hydration and norepinephrine continuous infusion. Results: Oral provocation tests were performed by using tamsulosin and ciprofloxacin. While tamsulosin did not evoke any symptoms, the patients had itching, erythematous rashes on the whole body 10 minutes after taking ciprofloxacin 62.5 mg and delayed hypotension (87/49 mmHg) occurred 5 hours later. He was diagnosed with delayed hypersensitivity to ciprofloxacin. Peripheral blood mononuclear cells isolated from the patient and normal controls were incubated with ciprofloxacin. The patient showed an increase in interleukin-5 and interleukin-13, while normal controls did not. Conclusion: This is the first case of ciprofloxacin-induced delayed anaphylaxis which was confirmed with oral provocation tests and in vitro cytokine analysis.

Postoperative outcomes of purely laparoscopic donor hepatectomy compared to open living donor hepatectomy: a preliminary observational study

Yu Jeong Bang,Joo Hyun Jun,Mi Sook Gwak,Justin Sangwook Ko,Jong Man Kim,Gyu Seong Choi,Jae Won Joh,Gaab Soo Kim 대한외과학회 2021 Annals of Surgical Treatment and Research(ASRT) Vol.100 No.4

Purpose: To lessen the physical, cosmetic, and psychological burden of donors, purely laparoscopic donor hepatectomy (PLDH) has been proposed as an ideal method for living donors. Our study aimed to prospectively compare the effect of PLDH and 2 other types of open living donor hepatectomy (OLDH) on postoperative pain and recovery. Methods: Sixty donors scheduled to undergo donor hepatectomy between March 2015 and November 2017 were included. Donors were divided into 3 groups by surgical technique: OLDH with a subcostal incision (n = 20), group S; OLDH with an upper midline incision (n = 20), group M; and PLDH (n = 20), group L. The primary outcomes were postoperative pain and analgesic requirement during postoperative day (POD) 3. Other variables regarding postoperative recovery were also analyzed. Results: Although pain relief during POD 3, assessed by visual analog scale (VAS) score and analgesic requirement, was similar among the 3 groups, group L showed lower VAS scores and opioid requirements than group M. Moreover, group L was associated with a rapid postoperative recovery evidenced by the shorter hospital length of stay and more frequent return to normal activity on POD 30. Conclusion: This pilot study failed to verify the hypothesis that PLDH reduces postoperative pain. PLDH did not reduce postoperative pain but showed faster recovery than OLDH.

국내산 산약의 품질특성 연구

Mi Hui Bang,Mun Gyu Lee,Min Woo Han,Yi Seong Kwak,Chae Kyu Park,Young Jong Lee,Jong Seong Kang 한국약용작물학회 2017 한국약용작물학술대회 발표집 Vol.2017 No.05

Background : This study of investigate the quality characteristics of Korean Dioscoreae Rhizoma by species and various processing methods. We used two types of species D. Rhizoma, which were the long type shape of D. batatas and short type shape of D. japoninca, respectively. Methods and Results : The reaction of D. Rhizoma with acetic acid and iodine showed light blue to blue colours, respectively. When compared with loss of drying between two species, the long type species was about 2% more higher than that of short type of species. When compared with processing methods, the both steamed and dried samples was more higher 2.0 - 3.0% than that of non-steamed samples. The contents of acid-insoluble ash samples with peel was relatively higher than that of samples containing no-peel. The results of TLC analysis also showed the same Rf and colours when compared with the standard of D. Rhizoma. However, both dried sample with peel (DWP) showed more clear spot than those of dried sample except peel (DEP) and both steamed and dried sample except peel (SDEP). Conclusion : We sincerely hope that this study will be contributed to the standardization and quality control of korean herbal medicines.

계속적 외래복막투석에서 여러가지 도관에 따른 도관관련합병증의 비교

김성호 ( Kim Seong Ho ),방종효 ( Bang Jong Hyo ),김동희 ( Kim Dong Hui ),임종우 ( Im Jong U ),조동규 ( Jo Dong Gyu ) 대한내과학회 1993 대한내과학회지 Vol.44 No.4

연구배경 : 계속적외래복막투석에서 도관출구감염, 누출, 전위 및 폐쇄 등의 고관관련합병증은 복막염과 같은 내과적 합병증과 함께 고관생존에 중대한 영향을 미친다. 1985년 Twardowski 등^(2)) 이 기존 straight Tenckhoff도관의 약점이었던 cuff돌출과 전위를 줄이기 위해 소위 swan neck 도관의 사용을 제시한 이래 swan neck도관은 현재 계속적외래복막투석에서 널리 사용되고 있다. 한편 도관의 피부 출구부분은 swan neck도관과 같이하되 도관의 복강내 끝부분을 또라이모양(curl) 으로 만들면 도관전위를 줄일 것으로 생각하여 curl bent neck도관이 고안되었으나 아직 그 효과에 대해서는 정설이 없다. 방법 : 103명의 환자를 대상으로 115예의 도관(straight Tenckhoff도관 56예, swan neck도관 30예, curl bent neck도관 19예)에서 도관생존율과 합병증의 발생율을 비교하였다. 결과 : 각 도관의 1개월, 3개월, 6개월, 12개월의 누적생존율은 straight Tenckhoff도관이 0.93, 0.91, 0.87, 0.83, swan neck도관이 0.87, 0.80, 0.73, 0.73, curl bent neck도관이 0.90, 0.90, 0.86, 0.79로 각 도관간의 누적생존율의 차이는 통계학적으로 유의하지 않았다. 합병증으로 인한 도관 제거는 모두 33예(straight Tenckhoff도관 20예, swan neck도관 8예, curl bent neck도관 5예)였으며 원인별로 보면 straight Tenckhoff도관에서는 복막염으로 12예, 전위 4예, 누출 및 폐쇄 각 1예, 기타 2예 모두 20예가 제거되었고, swan neck도관에서는 복막염 4예, 전위 2예, 출구감염 2예 모두 8예가 제거되었고, curl bent neck 도관에서는 복막염 2예, 누출, 전위 및 폐쇄 각각 1예 모두 5예가 제거되었다. 각 도관에서 도관관련합병증들의 발생율을 비교해보면 출구감염의 발생율은 straight Tenckhoff 도관, swan neck 도관, curl bent neck 도관에서 patient-month당 각각 0.044회, 0.051회, 0.036회, 도관전위는 0.024회, 0.032회, 0.046회, 누출은 0.008회, 0.009회, 0.021회, 도관 폐쇄 0.003회, 0회, 0.015회, 터널감염 0.002회, 0.003회, 0회, 탈장은 0.002회, 0.032회, 0회로 각각의 합병증의 발생율은 세가지 도관사이에 유의한 차이가 없었다. 복막염의 발생율은 patient-month 당 straight Tenckhoff도관 0.201회, swan neck도관 0.136회, curl bent neck도관 0.143회로 각 군간에 역시 유의한 차이가 없었다. 결론 : 도관생존율이나 도관관련합병증발생율에 있어서 어떤 도관이 더 유리하다고 보기는 어려울 것 같다. Background: The key to succeful chronic peritoneal dialysis is a permament and safe access to the peritoneal cavity. Despite of improvement in catheter suuvival over the last several years with reduction in the incidence of peritonitis, catheter-related complications still occur, causing significant morbidity and often forcing removal of the catheter. Methods: This study analysed the experience of 115 peritoneal dialysis catheters (56 straight Tenckhoff(ST), 30 swan neck(SN), and 29 curl bent neck (CBN) cateters) retrospectively, and compared 3 catheters with respect to catheter survival and catheter-related complication rate. Results: By life table analysis, the cumulative survival rate of each catheters was as follows: ST catheter were 0.93, 0.91, 0.87, and 0.83 at 1, 3, 6, and 12 month respectively, those of SN catheter 0.87, 0.80, 0,73, and 0,72, and CBN catheter 0.90, 0.90, 0.86, and 0,79. There was no significant difference among 3 types of catheter with respect to catheter survival. There were 33 catheter failuters (22 ST, 8 SN, and 5 CBN) due to peritonitis and catheter-related complications. The causes of catheter removal among 3 types of catheter are not different statistically. The exit site infection rate was 0.044case/patient-month in ST, 0.051 in SN, and 0.035 in CBN catheters. Dislocation rate was 0.024 in ST, 0.032 in SN, and 0.046 in CBN catheters. Leakage rate was 0.008 in ST, 0.009 in SN, and 0.021 in CBN catheter. Obstruction rate was 0.003 in ST, none in SN, and 0.015 in CBN catheters Tunnel infection rate was 0.002 in ST, 0.032 in SN, and 0.143 in CBN catheters. The peritonitis rate was not significant among 3 types of catheter. Conclusion: These results indicate that there is no difference among 3 types of peritoneal catheter with respect to catheter survival and catheter-related complications.

Changes of the Quality Characteristics in Korean Domestic Dioscoreae Rhizoma

Mi Hui Bang(방미희),Mun Gyu Lee(이문규),Min Woo Han(한민우),Yi Seong Kwak(곽이성),Chae Kyu Park(박채규),Young Jong Lee(이영종),Jong Seong Kang(강종성) 한국약용작물학회 2017 한국약용작물학회 학술대회논문집 Vol.2017 No.1

실험계획법을 이용한 최적 신경망 모델의 결정

이윤규(Lee Yun-Gyu),방윤경(Bang Yoon-Kyung),이성민(Lee Seong-Min) 대한토목학회 2001 대한토목학회논문집 C Vol.21 No.3