치주염의 원인균 및 정상상주균간의 길항기전 규명 및 치주치료에의 응용

손성희,정종평,김각균,김강주 대한구강생물학회 1991 International Journal of Oral Biology Vol.15 No.1

It has been reported that microbial distribution and colonization in oral cavity have a relation to antagonism, synergism or symbiosis among microorganisms and bacterial antagonism have a relation to bacteriocin, hydrogen peroxide and bacterial metabolic end-products. The purpose of this study is to investigate on the in vivo and in vitro ecological interrelation and to detect the growth inhibition subatance and to clarify the antagonistic mechanism of the periodontopathic bacteria. In vivo ecological interrelation was studied by bacterial isolation & identification and in vitro ecological was studied by stab culture method. Bacteriocin-like substance was examined, purified, electrophoresed, and stained with silver stain. After the producer microorganism was cultured in various conditions, the pH & bacterial end-products of it was examined and the susceptibility of indicator bacteria by pH was measured. The presence of plasmid DNA was investigated by hot-boiling method. In in vivo ecologic interrelationship, Actinobacillus actinomycetemcomitans(Aa) was not isolated, black-pig-mented Bacteroides was less in the presence of Actinomyces species than in the absence of it in refractory periodontitis. In in vitro ecologic interlationship, S. mutans showed strong growth inhibitory activity against Porphyromonas gingivalis and Aa strains and more strong growth inhibitory activity against Aa Korean isolates than S. sanguis. After bacteriocin-like substance was examined, purified, electrophoresed, and stained with silver stain, a band was appeared whose molecular weight was 36 kd, pH and the production rate of lactic acid was varied according to the presence and absence of oxygen and glucose. These results suggested that there exists in vivo and in vitro growth inhibition among oral microorganisms and growth inhibition was affected by bacteriocin-like substance, pH and bacterial end-products of producer strains and sensitivity of indicator strains. Animal study is needed by using strains of growth inhibition and growth inhibitory substance.

한국인 국소유년형 치주염환자의 치료 전후 임상, 미생물 및 면역학적 연구

손성희,정종평 대한구강생물학회 1989 International Journal of Oral Biology Vol.13 No.1

To investigate on the factors associated with disease progression of 9 early juvenile periodontitis, clinical, microbiological and immunological studies were performed at the mesial pocket of a first molar on the day of first visit, 9 months after observation without treatment and 6 months after treatment. Clinical parameters are included in sulcus bleeding index (S.B.I.), plaque index, alveolar bone resorption, and attachment loss. Microbiological studies are included in the proportion of bacterial morphotype and anaerobic culture of predominant cultivable microflora. Immunological studies are also performed with the assay of PMN chemotaxis dysfunction, and IgG, and IgM antibody levels to A. actinomycetemcomitans. Through subdivision of 9 subjects with non-progressing and progressing based on the changes in value of alveolar bone resorption and attachment loss, 1. Progressing group showes higher values of S.B.I than non-progressing group. 2. In bacterial morphotype analysis, the distribution of spirochetes are higher in the progressing group than non-progressing group based on alveolar bone resorption. 3. The proportion of anaerobic microflora shows higher in the progressing group than non-progressing group based on alveolar bone resorption. 4. A. actinomycetemcomitans revealed significantly higher frequency in progressing group than non-progressing group, and no detection after treatment. 5. There is no significant differences of PMN chemotaxis dysfunction between progressing and non-progressing group, and suggested to be no correlation with disease progression. 6. IgG antibody levels show significantly higher in non-progressing group than progressing group. However, IgM antibody levels show significantly higher in progressing group than non-progressing group based on alveolar bone resorption, and significantly reduced antibody levels after surgery. These results suggested that infection of A. actinomycetemcomitans and their negligible antibody levels might be relevant factors for progression of early juvenile periodontitis.

생약 추출물이 세포성장 및 cytokine 생산에 미치는 영향

류인철,손성희,정종평,배기환 충남대학교 약학대학 의약품개발연구소 1993 藥學論文集 Vol.9 No.-

The native connective tissue attachment of the periodontium is known to be a complex consisting of gingival fibroblasts, periodontal ligament cells, gingival epithelial cells, cementum, alveolar bone and extensive extracellular matrix (collagen, glycoprotein and proteoglycans). The purpose of this study was to evaluate the effects of natural extracts on DNA, collagen and protein synthesis and inhibition of cytokine production in the gingival and periodontal ligament fibroblasts and gingival epithelial cells. Healthy gingibal tissue was obtained from orthodontic treatment patients, and gingival epithelial cells, gingival fibroblasts and periodontal ligament cells were isolated and cultured from the samples. After treated with Ginseng protein, Pluronic F-68, Scutellariae Radix, centella asiatica, PDGF, IGF, DNA synthesis, total protein and collagen synthesis, and cytokine production of gingival epithelial cell, gingival fibroblast and periodontal ligament cells were measured. MTT method for DNA synthesis, Peterkofsky and Dingerman method for total protein and collagen synthesis, and IL-1 ELISA kit for cytokine production were used. The proliferation of epithelial cells was enhanced in Centella asiatica, Ginseng protein, Pluronic F-68 and Scutellariae Radix. The activities of PDL cells were increased in PDGF, IGF, and Pluronic F-68. Higher collagen synthesis was observed in Scutellariae Radix and total protein synthesis was increased in Scutellariae Radix and PDGF. The inhibitory effects on IL-1, IL-6, TNF-α were observed in all exrracts.

Pilocarpine 및 Atropine이 토끼의 악하선 혈류랑에 미치는 영향에 관한 연구

이종율,손성희,이종흔 대한치과페리오학회 1975 大韓齒周學會誌 Vol.5 No.1

정상 및 염증치은의 혈류량변동에 관한 연구

장계순,손성희,이종흔 대한치과페리오학회 1975 大韓齒周學會誌 Vol.5 No.1

한국인 연령에 따른 Actinobacillus actinomycetemcomitans의 발현빈도 및 항체 역가에 관한 연구

정종평,김강주,이상철,손성희 대한구강생물학회 1988 International Journal of Oral Biology Vol.12 No.2

In order to investigate on the frequency and antibody levels to Actinobacillus actinomycetemcomitans from Korean population, normal group were divided into 5 category by the related age : 3-6 years, 7-12 years, 13-20 years, 21-35 years and over 36 years, and also disease group were divided into the diagnostic condition : Localized juvenile periodontitis, post-localized juvenile periodontitis, rapidly progressive periodontitis, adult periodontitis. Each group was composed of 10 to 20 patients and plque sampling was also performed on the mesial pocket of mandibular lst molar with 3 paper points on anaerobic condition. Isolation and identification were done by using a selective medium(tryptic soy agar supplemented with 10% serum, 75ug of Bacitracin and 5ug of Vancomycin per ml) in 10% CO_2 incubator for 3 days with routine Gram staining, biochemical test and colony morphology. Serum antibody levels were also performed with isolated A. actinomycetemcomitans SNUDC 10-1 by using modified ELISA technique. The frequency of A. actinomycetemcomitans in normal healthy group was within 10% and no detection was revealed in 7 years group. Otherwise, the frequency of localized juvenile, rapidly progressive, adult perioduntitis and adult periodontitis associated with diabetes mellitus were 75%, 68%, 42%, and 25%, respectively. Serum IgG antibody levels to A.a SNUDC 10-1 in umbrical cord blood was similar to 3-6 years or 13-20 years group, and 3-6 months group were shown only trace amount of antibody levels. The antibody levels of 1 year and 7-12 years group were revealed 2/4-1/3 levels of 3-6 years group and this levels were gradually increased from 7-12 years group until over 36 years group. However, the antibody levels of localized juvenile, post-localized juvenile and rapidly progressive periodontitis were significantly higher than that of healthy group and no significant differences of the level were recognized between each disease group.

치주질환 병인균간의 성장억제 활성도에 관한 연구

정해원,이상철,정종평,손성희 대한구강생물학회 1988 International Journal of Oral Biology Vol.12 No.1

In a complex ecosystem such as periodontal pocket, bacterial antagonism may be a determinant in oral colonization and compositional changes of periodontopathic microflora. This study was performed to examine the growth inhibitory activities among the periodontopathic and indigenous bacteria. The growth inhibitory activity was examined as both producers and indicators by stab culture method. Inhibitory activities of cell sonicate and culture supernatant of S. mutans OMZ 65, which showed broad and strong inhibition against periodontopathic bacteria, was examined by the diffusion method. The results were as follows. 1. B. gingivalis, B. intermedius and A. actinomycetemcomitans strains showed weak inhibition against homologous and heterologous strains. Two among three strains of B. intermedius showed moderate inhibition against one strain of B. gingivalis and all of A. actinomycetemcomitans strains showed inhibitory activity to B. gingivalis. 2. The strains of S. mutans showed broad and strong inhibition against B. gingivalis, A. actinomycetemcomitans strains and moderate inhibition against B. intermedius, but S. sanguis strains showed weaker growth inhibitory spectra than those of S. mutans strains. However, the pattern of inhibitory activity showed, to a certain extent, species-specific. 3. S. mutans strains showed broader inhibitory spectrum against A. a. Korean strains than that of S. sanguis strains. But A. actinomycetemcomitans Korean strains has rarely showed growth inhibition against S. sanguis. 4. A. actinomycetemcomitans Korean strains showed weak inhibitory activity to B. gingivalis and no inhibitory activity to B. intermedius. 5. S. mutans showed strong inhibitory activity to homologous strains and typically species-specific than these of S. sanguis. Further investigation should be needed to clarify a relationship between the presence of plasmids and bacteriocin activity.

열공형과 비열공형 피질하 혈관성 치매에서 위험인자의 차이에 관한 비교 연구

배희준,정지향,유경호,나덕렬,김상윤,최경규,양동원,손의주,이상도,김재우,박경원,김응규,이재홍,박미영,한일우,함동석,최문성,하충건,최성혜,이애영,이병철,한설희 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.2

Backgrounds and Objectives: Vascular dementia is a group of dementing disoders arising from various stroke syndrome. Among these. subcortical ischemic vascular dementia (SIVD) is regarded as a relatively distinct clinical entity. However, MRI patterns of SIVD are not homogenous. In some patients, lacunes are dominant, and in others, subcortical white matter changes are. This study was designed to compare risk factor profiles between SIVD with and without multiple lacunes. Methods: We divided 47 subjects (22 males, mean age. 68 years) recruited from VADAPET (Multicenter Trial For Evaluation Of The Changes In the PET Images Of Subcortical Vascular Dementia Patient) study into two groups one with more than 5 lacunes in deep gray matter (lacune group) and the other with 5 or less(non-lacune group) Clinical characteristics and laboratory findings of two groups were compared. Results: Nineteen of 47 patients (40%) belonged to the lacune group. The lacune and non-lacune groups d d not differ in the following variables: age, hypertension, diabetes mellitus, hyperlipidemia heart disease, history of stroke or TIA, history of trauma or major surgery, family history of hypertension stroke, or dementia, age at diagnosis of dementia, body mass index, white blood cell count, ESR, CRP, fibrinogen, hemoglobin A1C, total cholesterol. LDL cholesterol creatinine, proteinuria, glucosuria, and microhematuria. However, male sex, smoking alcohol. hemoglobin, and HDL cholesterol were possibly associated more with lacune group SIVD than with non-lacune group (p<0 1) Multivariate analyses revealed that smoking, hemoglobin, and HDL cholesterol were independent predictors of SIVD with multiple lacunes Conclusion: Our study suggests that SIVD with multiple lacunes may be significantly different in smoking habits hemoglobin, and HDL cholesterol from SIVD without multiple lacunes.

Clinical and histological evaluation on the accelerated reattachment procedure in periodontal surgery

Son, Seong Heui 대한치과페리오학회 1977 大韓齒周學會誌 Vol.7 No.1

FREE AUTOGENOUS GINGIVAL GRAFT

Son Seong Heui 대한치과페리오학회 1975 大韓齒周學會誌 Vol.5 No.1