백서의 심실세동성 심정지 모델에서 해마신경세포 손상에 대한 단백질 합성저해제의 효과

오동렬,채장성,박승현,김세경,최세민,박제영 대한응급의학회 2000 대한응급의학회지 Vol.11 No.4

Background: The goal of successful resuscitation is not only to stop the process of ischemia as soon as possible but also to overcome the secondary injury process after resuscitation, which involves a complex interplay of mechanisms. Brain damage accompanying cardiac arrest and resuscitation is frequent and devastating. Cells die by one of two mechanisms: necrosis or delayed neuronal death. Delayed neuronal death may require protein synthesis. Neurons in the CA1 subfield of the hippocampus are selectively vulnerable to death after injury by ischemia and reperfusion. Death of these neurons occurs after an interval of 1 or 2 days. We assessed the effects of a protein synthesis inhibitor, cycloheximide(CHX), on hippocampal neuronal death of rats by using the ventricular fibrillation cardiac arrest(VFCA) model. Methods: The effect of CHX(3 mg/kg, s.c.) on hippocampal neuronal death was studied in two groups of 18 rats each, one group being subjected to a 2-min VFCA and the other to a 3-min VFCA. Each group was divided into three subgroups: control(groupⅠ,Ⅱ) without subcutaneous injection of CHX, "esp-12" of group Ⅰ/Ⅱ treated with CHX 12 hours after return of spontaneous circulation (ROSC), and "exe-24" of group Ⅰ/Ⅱ treated with CHX 24 hours after ROSC. The coronal sections of the hippocampus levels were stained with hematoxylin-eosin after 72 hours of survival. The histologic damage score(HDS) was used to assign a score to the total number of damaged neurons counted in each of the hippocampal CA1 subfields. Results: 1. There were not significant differences in heart rates, blood pressures, blood sugar, and blood gas in group I & Ⅱ during the pre-arrest steady state or at 5 min and 30 min after ROSC. 2. In group I & Ⅱ, the HDS, were significantly reduced in rats(I exp-12, 1.1 ±0.6;Ⅰexp-24, 1.3 ±0.5;Ⅱ exp-12, 1.4±0.7; and Ⅱ exp-24, 1.8±0.8) treated with CHX 12 hours or 24 hours after ROSC than control rats(1,2.5 ±0.9;Ⅱ,2.9±0.8)(p<0.05). Conclusion: These results suggest that delayed hippocampal neuronal death from ischemic insult after ventricular fibrillation cardiac arrest followed by resuscitation can be prevented by a protein synthesis inhibitor, CHX. Further experimental studies of the action mechanism of protein synthesis inhibitors to delayed neuronal death and clinical applications are required.

Effects of Industrial By-products on Reducing Heavy Metal Leaching in Contaminated Paddy Soil

Oh, Se Jin,Oh, Seung Min,Kim, Sung Chul,Ok, Yong Sik,Ko, Tae Yol,Ji, Won Hyun,Yang, Jae E. 한국토양비료학회 2015 한국토양비료학회지 Vol.48 No.1

Soil contamination with arsenic and heavy metals is a worldwide problem. Main objective of this research was to evaluated effects of reducing heavy metal leaching under reduced soil condition amended with industrial by-products. The contaminated soil was amended with 3% (w/w) of limestone (Ls), steel slag (SS) and acid mine drainage sludge (AMDS). Synthetic acid rain ($H_2SO_4:HNO_3=6:4$, pH 5.5 fixed) was used for feeding solution with flow rate of $0.78{\sim}0.88mL\;min^{-1}$. Results showed that similar pH and EC of leachate was observed in all treatments regardless of applied industrial by-products. However, arsenic concentration of leachate increased when industrial by-products were mixed. Meanwhile, concentration of heavy metal in the leachate decreased from 11.3 to 4.59 mg for Cd, from 92.3 to 7.93 mg for Pb, and from 11,716 to 1,788 mg for Zn via immobilization in soil with AMDS amended, respectively. Overall, application of industrial by-products can be an environmentally-friendly way to remediate soil and(or) leachate contaminated with metal(loid)s in metal mine site.

Effects of Industrial By-products on Reducing Heavy Metal Leaching in Contaminated Paddy Soil

Se Jin Oh,Seung Min Oh,Sung Chul Kim,Yong Sik Ok,Tae Yol Ko,Won Hyun Ji,Jae E. Yang 한국토양비료학회 2015 한국토양비료학회지 Vol.48 No.1

Soil contamination with arsenic and heavy metals is a worldwide problem. Main objective of this research was to evaluated effects of reducing heavy metal leaching under reduced soil condition amended with industrial by-products. The contaminated soil was amended with 3% (w/w) of limestone (Ls), steel slag (SS) and acid mine drainage sludge (AMDS). Synthetic acid rain (H₂SO₄:HNO₃ = 6:4, pH 5.5 fixed) was used for feeding solution with flow rate of 0.78~0.88 mL min-1. Results showed that similar pH and EC of leachate was observed in all treatments regardless of applied industrial by-products. However, arsenic concentration of leachate increased when industrial by-products were mixed. Meanwhile, concentration of heavy metal in the leachate decreased from 11.3 to 4.59 mg for Cd, from 92.3 to 7.93 mg for Pb, and from 11,716 to 1,788 mg for Zn via immobilization in soil with AMDS amended, respectively. Overall, application of industrial by-products can be an environmentally-friendly way to remediate soil and(or) leachate contaminated with metal(loid)s in metal mine site.

The Korean Association of lnternal Medicine : Poster Session ; PS 1357 : Nephrology ; Age is the Strongest Effector for the Relationship between Estimated Glomerular filtration Rate and Coronary Artery Calcifi cation in Apparently Healthy Korean Adults

( Hyun Beom Chae ),( Eun Jung Rhee ),( Hae Jung Park ),( Min Kyung Lee ),( Won Seon Jeon ),( Se Eun Park ),( Cheol Young Park ),( Won Young Lee ),( Ki Won Oh ),( Sung Woo Park ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Cardiovascular disease (CVD) is a major clinical problem and the most common cause of morbidity and mortality in patients with chronic kidney disease (CKD). Coronary artery calcifi cation (CAC) is a potential mechanism that explains the association between renal function and cardiovascular mortality. We aimed to evaluate the association between renal function and CAC in apparently healthy Korean subjects. Methods: In 23,617 participants in the health screening program in Kangbuk Samsung Hospital health screening program from January 2010 to December 2011, estimated glomerular fi ltration rate (eGFR) was assessed by Cockcroft-Gault equation. Coronary artery calcium score (CACS) was measured by multi-detector computed tomography (MDCT). Subjects were divided into 3 groups according to the CKD Staging system with eGFR grade; stage 1: eGFR = 90 mL/min/1.73 m2, stage 2: eGFR 60-89 mL/ min/1.73 m2, stage 3: eGFR 30-59 mL/min/1.73 m2. Results: Mean age of the participants was 41.4 years, and mean eGFR was 103.6±21.7 mL/min/1.73 m2. Hypertension and diabetes were noted in 43.7% and 5.5% of the participants, respectively. eGFR showed weakly negative but signifi cant association with CACS in bivariate correlation analysis (r=-0.076, p<0.01). Mean CACS signifi cantly increased from CKD stage 1 to 3. The proportion of subjects who had CAC signifi cantly increased from CKD stage 1 to 3. Although odds ratio for CAC signifi cantly increased from stage 1 to 3 after adjustment for confounding factors, this signifi cance was inversed when age was included in the model. Age was the strongest factor that affects CAC. Conclusions: This study performed in young Korean subjects with relatively normal renal function, age was the mostly strongest effector on the association between eGFR and the degree of CAC.

턱끝절골술 : 189명에 대한 고찰

오갑성,백룡민,이태일,임재호,백세민 대한미용성형외과학회 1997 Archives of Aesthetic Plastic Surgery Vol.3 No.1

The chin plays a decisive role in the contour of the lower face. And harmonious jaw will correspond to good facial profile and positive image. Osseous genioplasty is a widely used method to correct the 3-dimensional(sagittal, vertical, transverse) deformity of the chin with minimal complications. During an 8 year period from 1988, the authors at the In-Je University Seoul Paik Hospital experienced 198 patients of osseous genioplasty. Results of clinical analysis are as follows: 1)Mail to female ratio was 1 : 3 with female being predoniment. The average age was 24.9 years. 2) Horizontal advancement genioplasty in 169 cases was the most common precedure with 7.0mm of average advancement. There was 4 cases of horizontal retrusion with average retrusion of 4.3mm. 52 cases had vertical augmentation with average of 7.5mm increase. 9 cases had vertical reduction with average redution of 4.9mm. 3) Although malocclusion is not necessarilly contraindication to genioplasty alone, genioplasty following surgical with or without orthodontical correction of malocclusion is the way to get better result with patient satisfaction. 4) 77% of the patients had adjunctive procedures simultaneously to acheive a better contour and profile 5) Complications such as hematoma, infection, fracture, nerve damage were minimal.

지도자 유형이 태권도 수련생의 사회성 발달에 미치는 영향

오세용,김민환,이창민 한국스포츠리서치 2005 한국 스포츠 리서치 Vol.16 No.2

This study aims at exploring the effect of leadership type on Taekwondo trainees' development in sociality. in order to help as a reference for a Taekwondo training. The measuring instrument(Leadership Scale for Sport) used in the survey was developed by Chellandurai and Saleh(1980) and revised by Kim(1990). Items related with development in sociality argued by Ahn(1997) and Yeon(2001) were also used. Data were processed through SPSS. Frequency analysis was done to figure out background variables of the objects. Reliability analysis was done to verify the questionnaire reliability. Multiple regression was used to explore the effect of leadership behaviors on the trainees' development in sociality. The results were as follows Firstly, social supporting highly affected sub-factors of sociality development such as trust, justice. and patriotism. Secondly, positive rewarding behaviors highly affected sub-factors of trainees' sociality such as language use habits, responsibility. Thirdly, training and instructing behaviors by the trainers highly affected trainees' teamwork. When synthetically looking, it was found that trainees' development in sociality had various sub-factors as did the trainers' types. Accordingly, it was thought that proper custom-made training in developing sociality was important to trainees.

안와골절의 외과적 접근에 대한 증례보고

민승기,이은택,오승환,이동근,고세욱,송종민,최성림 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.3

The Orbital fracture which is often combined with midface fracture can cause decreased visual acuity, limitation of eyeball movement, diplopia, enophthalmos, etc. Traumatic orbital fracture causes change of orbital volume, results in diplopia and enophthalmos thus, accurate repositioning of displaced bone and reconstruction of orbital defect with autogenous materials(cranial, rib, iliac bone, cartilage and fascia lata) or alloplastic materials(gelatin film, polyglactin mesh, methylmethacrylate, Teflon, silicone, Supermid, hydroxyapatite and metal). The key point of reconstruction of orbit is accurate repositioning of displaced orbital floor, lateral, medial orbital wall and sufficient bonegraft in anatomical defect. As this cases, we obtained good results through transconjunctival and coronal approach who were required orbital reconstruction. Also, we accurately diagnosed orbital fracture with C.T., 3D model and good result for orbital reconstruction with sufficient parietal block bone graft during average 16.8 months follow-up.

경기도 용인시(경희대학교) 주변지역의 지하수 오염현황

오종민,김홍석,진현오,윤세철 경희대학교 환경연구소 1998 環境硏究 論文集 Vol.8 No.-

Recently, the concern about groundwater pollution is increasing. And, groundwater was one of the major water resource used as drinking water by eighty percent of the world population, and its utility as industry water, agriculture water, fresh water was increasing. But, when pollution material was found in housing well, by purifying polluted water, the area of doing thorough conservation management was few. Thus, if water resource being regard as unlimited source, the right view of nature and scientific utility were essential because of the problem of its value. The purpose of this study was that investigated groundwater quality pollution condition in the part areas of Kyunggi-do Suwon Si, Yong-In Si, proposed to efficient management plan for pollution occurrence prevention and groundwater quality development by surveying groundwater pollution source. The result of the study was that the groundwater by use purpose could systematically manage water quality since development beginning, management system about groundwater development with use can established.

이종재료의 레이저용접에서 잔류응력 평가

오세헌,민택기 한국공작기계학회 2004 한국생산제조학회지 Vol.13 No.3

Generally, it is used the compensation spring to compensate the inaccuracy of screen image induced by thermal deformation in CRT monitor. Its mechanism is bi-metallic system made of heterogeneous metals and these is bonded by laser welding. But laser welding induces the non-uniform temperature distribution and locally residual stress is yielded by these temperature deviation. This paper studies residual stress of laser weldment using FEA and hole drilling method. The results are followed. In the case of heterogeneous materials weldment, higher residual stress induced in the weldment region of SUS 304 which have larger CTE than Xi 36 and residual stress on the middle of specimen is higher by 10.9% than that of its surface Measured residual stress of SUS 304 yield 481MPa and that of Ni 36 is 140.5MPa in the vicinity of the welding region. And the residual distribution is very similar in comparison with FEA result.

Calcium Carbide 處理時期가 Pineapple의 開花誘導, 生育 및 收量에 미치는 影響

吳世珉,姜榮吉 濟州大學校 亞熱帶農業硏究所 1987 亞熱帶農業硏究 Vol.4 No.-

無加溫施說栽培 Pineapple의 人爲開花誘導를 위한 CaC₂處理 適期를 究明하고자 1985年 5月 23日에 栽植한 Special Amarello 品種에 1986년 5月 21日부터 9月 31日까지 21일 間隔으로 6回에 걸쳐 3%의 水溶液을 生長點에 株當 30㎖식 灌主하여 開花率, 處理後 抽苔 ·成熟까지의 日數, 生育 및 果實 特性을 調査한 結果를 要約하면 다음과 같다. 1. 開花率은 無處理區에서 13%인데 비하여 處理區에서는 87∼100%였다. 2. 處理 後 抽苔까지의 日數는 7月 2日 處理區에서 65日로 가장 짧았고 8月 13日과 9月 3日 處理區에서는 越冬 後 抽苔가 되어 各各 219, 232日이었으며, 處理 後 抽苔까지의 日平均 平均氣溫, 日平均 最低氣溫, 日平均 有效溫度와 高度를 有意한 負의 相關이 있었다. 3. 處理 後 成熟까지의 日數는 5月 23日 處理區까지는 228∼236日이었으나 8月 13日 과 9月 3日 處理區에서는 各各 351, 359日로 크게 增加되었다. 4. 成熟期의 草長 및 葉數는 處理時期가 늦을 수록 增加되었다. 5. 株當 吸芽數 및 裔芽數, 冠芽長은 CaC₂ 處理에 의하여 크게 減少되었으나 處理時間 間에는 有意差가 없었다. 6. 果便은 處理時期가 늦어질 수록 짧아지는 傾向이였다. 7. 果長, 果徑, 果重, 小果數 모두 5月 21日∼ 7月 21日 까지의 處理區에서는 無處理區나 7月 23日∼ 9月 3日 處理區보다 컸었다. Calcium carbide solution (30g/1 liter water) was applied to the center of pineapple (Ananas comous; cv Special Amarello) planted on May 23. 1985 at 30 ml per plant on May 21, June 11 July 2, July 23, August 13, and September 3, 1986 to determine effects of calcium carbide application date on floral induction, the number of days from the treatment to maturity, fruit size and weight of pineapple grown in plastic film house in Cheju province. The results obtained are as follows; 1. Calcium carbide application induced 87 to 100% flowering compared with 13% flowering of untreated plants. However, application date did not significantly affect flowering percentage of treated plants. 2. The number of days from treatment to inflorescence emergence was 65-91 days for plants treted on May 21 to July 23 and 219 and 232 days, respectively, for plants treated on August 13 and September 3. The number of days from treatment to inflorescence emergence was highly, negatively correlated with average mean air temperture (unit GDD-1), average daily minimum air temperature (unit GDD-2) or unit growing degree days (unit GDD-3) from treatment to inflorescence emergence. 3. The number of days from treatment to maturity was 228 to 236 days for plants treated on May 21 to July 23, and 351 and 359 days, respectively, for plants treated on August 13 and September 3. 4. Plant height and the number of active leaves per plant at maturity increased with delaying application of calcium carbide. 5.The number of suckers and slips per plant and crown length at maturity was decreased by application of calcium carbide and was not significantly affected by appliaction date of calcium carbide. 6. Penduncle length tended to decrease with delaying application of calcium carbide. 7. Fruit length and diameter, fruit weight and the number of fruitlets per fruit were greater for plants treated on from May 21 to July 2 than for plants treated on from July 23 to September 3.