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임신부 뇨로부터 정제된 인간 상피세포 증식 인자 유사체의 in vitro bioassay 및 특성
박세철,전재현,남정현,권태종,고인영,유광현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4
벤조산흡착, 음이온 교환수지, 단일클론항체을 이용한 immunoaffinity chromatography를 통하여 임신부 뇨로부터 천연의 hEGF를 정제하였다. 정제된 hEGF는 μ Bonda C_18 column을 사용한 HPLC 분석을 통하여 4개의 fraction으로 분리가 가능하였으며 western blot과 double immunodiffusion 실험 결과, 각각의 fraction이 hEGF의 특성을 가진 유사체인 것을 알 수 있었다. 또한 hEGF 표준 물질과의 spiking 및 아미노산 분석 등을 통하여 두 번째 fraction이 nhEGF와 동일한 것으로 확인하였다. nhEGF 및 그 유사체의 생물학적 활성 비교를 위하여 NIH 3T3 세포주에서 5'-Brdu incorporation 측정을 위한 labelling 시간, 혈청 농도의 최적 조건을 결정하였다. NIH 3T3 세포주의 DNA 합성능은 0.2% FCS가 포함된 저혈청 배지에서 hEGF가 0.1~10 ng/ml 농도로 첨가하였을 때 증가하는 경향을 나타냈다. HPLC를 통하여 분리된 두번째 hEGF 유사체가 다른 유사체보다도 생물학적인 활성이 우수하였으며, rhEGF 표준물질과의 spiking 및 아미노산 서열 분석등을 통하여 nhEGF로 밝혀졌다. 임신부의 뇨의 hEGF 유사체 함량중 natural hEGF는 46%이었다. Natural human epidermal growth facto (nhEGF) was purified from pregnant human urine by benzoic acid adsorption, DEAE-Sepharose ion exchange, and immunoaffinity chromatography. The purified nhEGF was further separated into four fractions using Bondapak C_18 HPLC system. Following characterization by Western blot and double immunodiffusion, we found that each fraction corresponds to four derivatives of the nhEGF. For biological analysis of nhEGF, we optimized the labeling time and serum concentration for the incorporation of 5-bromo-2'-deoxy-uridine (BrdU), a non-radioactive alternative for [^3H]-thymidine uptake, into NIH 3T3 cells. The DNA synthesis of NIH 3T3 cells was gradually increased at the nhEGF concentrations between 0.1~10 ng/ml in the Dulbecco's Modified Eagles Medium (DMEM) containing 0.2% Fetal calf serum (FCS). When we assayed the biological activity of four fractions, the activity of the second fraction was superior to that of the others.
바이오 디젤유를 차량 적용 시 입자 배출가스 특성에 관한 연구
김세준,정민원,김병준,유종식,차경옥 明知大學校 産業技術硏究所 2007 産業技術硏究所論文集 Vol.26 No.-
In this study, diesel passenger vehicle were measured on. chassis dynamometer test bench. this base engine was investigated by number measurement. so the particulate matter and gaseous emission were measured each test vehicle, fuel, mode condition on chassis dynamometer test bench that using the apparatus recommended by PMP(Particle Measurement Program) A condensation particle counter (CPC) system was applied to measure the particle number and size concentration of diesel exhaust particles at the end of dilution tunnel along the NEDC vehicle test mode. The effects of Bio diesel fuel on the Diesel nano particle number and size distribution was investigated in this study.
디젤승용 차량에서 배출되는 가스상 물질과 극미세 입자상 물질을 차량시험모드에 따른 실험적 분석
김세준,정민원,김병준,유종식,차경옥 明知大學校 産業技術硏究所 2007 産業技術硏究所論文集 Vol.26 No.-
Recently, the nano-PM's number concentration emitted by diesel internal combustion engine has drawn attentions because this particulate matters are suspected being hazardous of human health. In this study, The nano-PM mass and size of diesel passenger vehicles were measured on chassis dynamometer test bench. The particulate matters (PM) emissions of these vehicles were investigated for number concentration too. A condensation particle counter (CPC) system was applied to measure the particle number and size concentration of diesel exhaust particles at the end of dilution tunnel along the NEDC(ECE1 5+EUDC) and CVS-75 vehicle test mode. As the research result, the characteristic of vehicle test mode on the diesel nano-particle number and size distribution was investigated in this study.
반복가열냉각에 의한 수화시멘트 페이스트와 골재 분리 방법
김세훈,임승준,안재철,오상균,김정길 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)
Cement mortar and efficient disconnection of aggregate are indispensable for recycling of waste concrete, and the problem is with quality in the existing physical disconnection way, and the problem has been by application of a concrete recycling construction materials. Specially, in case of recycled aggregate, the quality is lowered because of the fine aggregate powder which has a bad influence on flowability, a compression strength by adhesion of mortar on the aggregate face, and the case of recycled cement was added to during recycle cement. Therefore, a mortar and interfacial separation of aggregate are large in proper quality for concrete recycling is expected that affect. This study based on the recycled cement manufacturing way which used an aggregate interfacial separation of waste mortar in this study so that the coefficient of thermal expansion of hardend cement paste and an the coefficient of thermal expansion of a fine aggregate are different in a recycled cement manufacturing way, but leaves a purpose.
임승준,김세훈,오상균,김정길,강병희 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)
Serious problems of the environment protection and resource exhaustion are exhibited, due to the increase of the construction materials and activation of the remodeling, recently. Especially, most of the advanced countries, recycling plan for the waste concrete is vigorously progressing. The recycle of domestic waste concrete is, however, still in an early stage, and it has been only partially being used for the road fillers. As a counter-plan of activating recycled concrete, we have confirmed the hydration possibility of the waste concrete powder from the experiment on recycling the aggregate powder since 2000. Also, we have known that the strength is increasing when the heating time is longer, and baking temperature maintain in 700℃. The purpose of this study is to determine the most suitable production method controlling the duration of baking time for recycled cement from the compressive strength, X -ray diffraction and ingredient analysis test.
발목관절 중립위치와 발바닥 굽힘 위치간 스쿼트 운동 시의 하지근육 및 척추기립근 근활성도 비교: 예비 실험 연구
성하림,오세정,차용준 대전대학교 기초과학연구소 2019 自然科學 Vol.30 No.-
The purpose of this study was to compare muscle activity of lower extremity muscle and erector spine muscle between ankle joint neutral position and plantar flexion position. The muscle activity of the vastus medialis oblique, vastus lateralis, biceps femoris, and erector spinae were measured according to the two positions of ankle joint neutral position and ankle joint plantar flexion during squat exercise in 20 normal adults. The muscle activity of the vastus medialis oblique, vastus lateralis, biceps femoris, and erector spainae were significantly different according to the position of the ankle joint during squat exercise (p <.05). In the vastus medialis oblique, the ankle plantar flexion showed higher muscle activity than the ankle joint neutral position (73.9% of MVIC vs. 78.8 of MVIC). Ankle joint neutrality showed higher muscle activity than ankle joint flexion in the vastus lateralis, biceps femoris, and erector spinae (75.1% of MVIC vs. 69.4% of MVIC; 7.7% of MVIC vs. 6.7% of MVIC; 25.9% of MVIC vs. 21.9% of MVIC, respectively). The plantar flexion of the ankle joint during squat exercise is a more effective way to strengthen the vastus medialis oblique, but it is less effective than the ankle joint neutral position for strengthening the vastus lateralis, biceps femoris, and erector spinae.
생체모사환경에서 DLC 필름의 부식마모 특성에 미치는 Si 함량의 영향에 관한 연구
김호건,김우중,김정구,박세준,이광렬 대한금속재료학회 2005 대한금속·재료학회지 Vol.43 No.3
In a method to improve resistance to wear, corrosion and mechanical properties of protective coating, DLC coatings have been extensively studied over the past several years due to their unique combination of properties. Especially, Si-DLC coatings have been attracting increasing interest of researchers since they have a great potential for solving some of the major disadvantages of pure DLC coatings. Si-incorporated diamond-like carbon (Si-DLC) coatings ranging from 0 to 2 at.% were deposited on Ti alloy substrate by means of r.f. plasma-assisted chemical vapor deposition (r.f. PACVD) technique, using mixtures of benzene (C_(6)H_(6)) and silane (SiH_(4)) as the reaction gases. Corrosion and wear behavior of DLC coatings was investigated by electrochemical techniques (potentiodynamic polarization test and electrochemical impedance spectroscopy), tribological techchnique(wear test) and surface analyses (atomic force microscopy, scanning electron microscopy and Raman spectroscopy). It was found that DLC coatings with increasing Si content could acquire improved corrosion and wear resistance in the simulated body fluid environment (0.89 wt.% NaCl of pH 7.4 at 37℃). (Received December 6, 2004)