Estimated incidence of juvenile-onset recurrent respiratory papillomatosis in Korea

Jin-Kyoung Oh,Hwa Young Choi,Minji Han1,Yuh-Seog Jung,Sang Joon Lee,Moran Ki 한국역학회 2021 Epidemiology and Health Vol.43 No.-

OBJECTIVES: Recurrent respiratory papillomatosis (RRP) is caused by human papillomavirus (HPV) types 6 and 11 and is potentially preventable through vaccination. This study estimated the incidence of juvenile-onset RRP before the implementation of the national HPV vaccination program in Korea. METHODS: We conducted a cohort study using claims data provided by a mandatory insurance program to estimate the incidence of RRP and associated healthcare use. Patients with juvenile RRP were defined as those aged ≤12 years with ≥2 admissions or ≥2 outpatient visits during which they received the International Classification of Diseases, 10th revision code for benign neoplasms of the larynx (D14.1). RESULTS: During 2002-2014, 123 children (74 boys and 49 girls) were diagnosed with RRP. The patients had a mean of 6.5 person-years of follow-up. The incidence was estimated at 0.30/100,000 person-years. The median age at diagnosis was 4.0 years (mean, 4.3). Thirty-six (29.3%) patients underwent surgery, including 23 patients (18.7%) who underwent 2 or more surgical procedures. Severe disease, measured by more frequent surgical procedures and shorter time intervals between consecutive operations, was associated with a younger age at diagnosis. CONCLUSIONS: The estimated incidence of juvenile-onset RRP in Korea was similar to that reported in other countries. The RRP burden should continue to be monitored using National Health Insurance Service claims data.

Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

일학습병행에서 학습기업의 재참여 의사에 미치는 영향 요인 탐색

조한진(Jo, Han-Jin),이선화(Lee, Seon-Hwa),박종성(Park, Jong-Sung) 한국경영교육학회 2020 경영교육연구 Vol.35 No.2

[연구목적] 기존 일학습병행에 참여한 학습기업의 재참여 활성화 방안에 앞서 학습기업의 재참여에 미치는 영향 요인을 구명하여, 학습기업 재참여 활성화 방안에 정책적 기초자료를 제공하는 것이다. [연구방법] 독립변수는 총 11개로 범주형 4문항과 연속형 7문항으로 구성되어 있고, 종속변수는 범주형 1문항으로 구성되어 이분형 로지스틱 회귀분석을 적용하였다. [연구결과] 전체적으로 학습기업의 재참여 의사에 영향을 미치는 요인은 참여 동기, 일학습병행 전반적인 만족, 현장훈련 시간 단축 필요성, 기업현장교사 양성교육 기간 적절성, 일학습병행 훈련과정 종료 후 유지 계획 유무, 참여유형의 6가지 변수로 나타났다. 참여유형을 재직자단계와 재학생단계로 나누어 분석한 결과는 다음과 같다. 첫째, 재직자단계에서 학습기업의 재참여 의사에 영향을 미치는 요인은 참여 동기, 일학습병행 전반적인 만족, 현장훈련 시간 단축 필요성, 기업현장교사 양성교육 기간 적절성, 일학습병행 훈련과정 종료 후 유지 계획 유무의 5가지로 나타났다. 둘째, 재직자단계에서 학습기업의 재참여 의사에 영향을 미치는 요인은 참여 동기, 학습기업 선정 절차 적절성, 학습기업 선정기준 적절성, 일학습병행 전반적인 만족, 일학습병행 훈련과정 종료 후 유지계획 유무의 5가지로 나타났다. [연구의 시사점] 일학습병행의 내실화를 위하여 학습기업의 재참여 활성화 방안을 위한 정책적 시사점을 제공하였다. [Purpose] The purpose of this study is to examine the factors affecting re- participation of companies involved in apprenticeship before the revitalization of re-participation and to provide basic policy data to revitalize re-participation of companies involved in apprenticeship. [Methodology] There were 11 independent variables comprising four questions for categorical variables and seven questions for continuous variables. One dependent variable comprised one question for a categorical variable. The binomial logistic regression analysis was applied. [Findings] The factors affecting re-participation intention of companies involved in apprenticeship were six variables, including the motivation to participate, overall satisfaction about apprenticeship, need for the shortened time of on-the- job training, appropriate training period of in-company trainers, plan to maintain apprenticeship after the end of the training process, and participation type. The results of analyzing participation type First, there were five factors affecting re-participation intention of companies involved in the apprenticeship for students, including the motivation to participate, overall satisfaction about apprenticeship, need for the shortened time of on-the-job training, appropriate training period of in-company trainers, and plan to maintain apprenticeship after the end of the training process. Second, there were five factors affecting re-participation intention of companies involved in the apprenticeship for new workers, including the motivation to participate, appropriate procedure for selecting companies involved in apprenticeship, appropriate criteria for selecting companies involved in apprenticeship, overall satisfaction about apprenticeship, and plan to maintain apprenticeship after the end of the training process. [Implications] Policy implications were presented to substantialize apprenticeship and to revitalize re-participation of companies involved in apprenticeship.

Assessment of In Vitro Assay System for Thyroid HormoneDisruptors Using Rat Pituitary GH3 Cells

Hee Jin Kim1,Hae Young Park1,Jeonga Kim1,Il Hyun Kang2,Tae Sung Kim2,Soon Young Han2,Tae Seok Kang2,Kui Lea Park2,Hyung Sik Kim1 한국독성학회 2006 Toxicological Research Vol.22 No.4

The development of in vitro assays has been recommended to screening and test-ing the potential endocrine disruptors (EDs). These assay systems focus only on identifying thethe thyroid hormone (TH) disruptors. The aim of this study was to evaluate a test system to detectTH disruptors using rat pituitary tumor GH3 cells. The test system is based on the TH-dependentincrease in growth rate. As expected, L-3,5,3-triiodothyronine (T3) markedly induced a morphologicalchange in GH 3 cells from flattened fibroblastic types to rounded or spindle-shaped types. T3 stimu-lated GH3 cell growth in a dose-dependent manner with the maximum growth-stimulating effect9 M. In addition, T3 increased the release of growth hor-mone and prolactin into the medium of the GH3 cells culture. Using this assay system, the TH-dis-rupting activities of bisphenol A (BPA) and its related compounds were examined. BPA,dimethylbisphenol A (DMBPA), and TCI-EP significantly enhanced the growth of GH3 cells in therange of 1 × 10-5M to 1 × 10-6M concentrations. In conclusion, this in vitro assay system might bestandardization before it can be used as a broad-based screening tool.

병자호란 전후(1636-1638) 소의 역병(牛疫) 발생과 확산의 국제성

김동진(KIM Dong Jin),유한상(YOO Han Sang) 대한의사학회 2013 醫史學 Vol.22 No.1

This study reviewed the outbreak and prevalence of cattle plague around Byeongjahoran from the perspective of international war in East Asia. First of all, the epidemiological characteristics of cattle plague in Manchuria where the outbreak of cattle plague was first reported around Byeongjahoran were analyzed. From the study, it was found the military activities that Sarhu (Qing) had made during the growth into Empire promoted the exchanges of various germs which became naturalized in the regions in Northeast Asia, and that such extreme situation as war made various diseases taken place and dispersed. In particular, because of military activities of Sarhu (Qing), various contagious diseases including smallpox which was prevalent in Inner-Mongolia and Shanxi became prevalent in Manchuria. During the contacts with Chosun after Jeongmyohoran, pathogen occurring Rinderpest was introduced into Manchuria. Favorable conditions for the interactions with various pathogens were provided by frequent contacts with wild animals through hunting and various cultivation groups composed of Manchurians, Mongolians, Han-Chinese and Chosun people. Rinderpest breaking in Chosun around Byeongjahoran was originated in Shenyang in 1636. It was transmitted to cattle in the Korean Peninsula and expanded to Kansai Region. At that time Rinderpest occurred and rapidly expanded in a specific area due to the interactions of pathogens, hosts and environments, and suddenly disappeared because of the extinction and the separation of hosts. It is consistent with the symptoms of modern times ‘Rinderpest.’ In Chosun it occurred in Pyeongan-do 4 months before the outbreak of Byeongjahoran and gave great damage on the capital area and northern Gyeonggi region. Because of the large scale migration of militaries after Byeongjahoran, Rindpest was expanded to Hasamdo and was terminated in February to April leaving big damages. The damages of Byeongjahoran were very severe. From the statistical records, it was found that the mortality rate in Gyenggi-do was around 2/3, around 50% in Jeju area. The mortality rate of infected cattle was around 75%. In some records based on individual cases, 80-100% of mortality rate was addressed. It is comparable to 25% of mortality in 1627, and is near or less than the mortality rate of Rinderpest in the 19th and 20the century. When analyzing the expansion of Rinderpest from the perspective of place, the most damaged places were areas near the busy roads or the places with dense population. Therefore, the remote places far from busy roads or separated from the affected places right after the outbreak did not have much damage. Additionally, rich stock-feeders (豪民) had relatively small damages and poor households (小民) with 1 or 2 stokes were badly affected. The prevention and supply of medication by government made considerably positive effects on the prevention and treatment of Finderpest.

Hypoallergenic and Physicochemical Properties of the A2 β-Casein Fraction of Goat Milk

Tae-Hwan Jung,Hyo-Jeong Hwang,Sung-Seob Yun,Won-Jae Lee,Jin-Wook Kim,Ji-Yun Ahn,Woo-Min Jeon,Kyoung-Sik Han2.6* 한국축산식품학회 2017 한국축산식품학회지 Vol.37 No.6

Goat milk has a protein composition similar to that of breast milk and contains abundant nutrients, but its use in functional foods is rather limited in comparison to milk from other sources. The aim of this study was to prepare a goat A2 β-casein fraction with improved digestibility and hypoallergenic properties. We investigated the optimal conditions for the separation of A2 β-casein fraction from goat milk by pH adjustment to pH 4.4 and treating the casein suspension with calcium chloride (0.05 M for 1 h at 25°C). Selective reduction of β- lactoglobulin and αs-casein was confirmed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis and reverse-phase high-performance liquid chromatography. The hypoallergenic property of A2 β-casein fraction was examined by measuring the release of histamine and tumor necrosis factor alpha from HMC-1 human mast cells exposed to different proteins, including A2 β-casein fraction. There was no significant difference in levels of both indicators between A2 β-casein treatment and the control (no protein treatment). The A2 β-casein fraction is abundant in essential amino acids, especially, branched-chain amino acids (leucine, valine, and isoleucine). The physicochemical properties of A2 β-casein fraction, including protein solubility and viscosity, are similar to those of bovine whole casein which is widely used as a protein source in various foods. Therefore, the goat A2 β-casein fraction may be useful as a food material with good digestibility and hypoallergenic properties for infants, the elderly, and people with metabolic disorders.

Characteristics of Prolonged Anode - type Metal Ion Source

Sung Han,Sung-Chang Choi,Ki-Hwan Kim,Ki-Hyun Yoon,Won-Kook Choi,Hyung- Jin Jung,Seok-Keun Koh 한국진공학회 1998 한국진공학회 학술발표회초록집 Vol.- No.-

Surface Compositional Changes of Polyethylene, Polyvinylidenefluoride, and Polytetrafluoroethylene modified by a keV irradiation

Sung Han,Ki-Hyun Yoon,Won-Kook Choi,Hyung-Jin Jung,Seok-Keun Koh 한국진공학회 1998 한국진공학회 학술발표회초록집 Vol.- No.-

The Analysis of the Muscle Activity in Individual Work Sections of the Sowing Process

Sang Mi Lee,Gyung Mee Gim,Seon Hee Jeong,Sun Jin Jeong,Yoonah Jang,Kyung Sook Han,Hyun Jin Jang 한국인간·식물·환경학회 2017 인간식물환경학회지 Vol.20 No.4

This study was conducted to analyze the muscle activity in the right forearm and shoulderwhile doing the sowing activity by muscle type and work section, and to find the possibility of the utilization of sowing as a muscle exercise and rehabilitative activity based on the results of the analysis. The results showed that the activity of 7 muscles including the upper trapezius near the neck, the upper trapezius near the shoulder, the middle deltoid, the biceps brachii, the triceps brachii, the brachioradialis, and the flexor carpi ulnaris were significantly higher than the stable state. In addition, the muscle activity in the sections of mixing the soil, filling the tray with the soil, sowing, writing the label, and watering was significantly higher than the stable state. In particular, the muscle activity of the biceps brachii and the triceps brachii was statistically significantly high in the section of filling the tray with the soil, which indicates that the task of filling the tray with the soil can be utilized as an exercise to activate the biceps brachii and the triceps brachii. In addition, the muscle activity of the brachioradialis was significantly high in all the work sections, which indicates that the sowing process can be utilized to exercise the brachioradialis in particular. These results can be used as the basic data about the activated muscle types and degrees throughout the sowing process. These results can be also used to induce the activation of specific muscles, and the overall exercise and rehabilitation of the forearms.

A High-Lateral Resolution MALDI Microprobe Imaging Mass Spectrometer Utilizing an Aspherical Singlet Lens

Han, Sang Yun,Kim, Hwan Jin,Ha, Tae Kyung Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.1

We report the construction of a MALDI imaging mass spectrometer equipped with a specially designed laser focusing lens, a compact aspherical singlet lens, that obtains a high-lateral imaging resolution in the microprobe mode. The lens is specially designed to focus the ionization laser (${\lambda}$ = 355 nm) down to a $1{\mu}m$ diameter with a long working distance of 34.5 mm. With the lens being perpendicular to the sample surface and sharing the optical axis with the ion path, the imaging mass spectrometer achieved an imaging resolution of as good as $5{\mu}m$ along with a high detection sensitivity of 100 fmol for peptides. The mass resolution was about 900 (m/${\Delta}m$) in the linear TOF mode. The high-resolution capability of this instrument will provide a new research opportunity for label-free imaging studies of various samples including tissues and biochips, even for the study at a single cell level in the future.