Antioxidant and anti-inflammatory activity of Heritiera fomes (Buch.-Ham), a mangrove plant of the Sundarbans

Islam Md. Emdadul,Islam Kazi Mohammed Didarul,Billah Md. Morsaline,Biswas Rana,Sohrab Md. Hossain,Rahman S. M. Mahbubur 경희대학교 융합한의과학연구소 2020 Oriental Pharmacy and Experimental Medicine Vol.20 No.2

The antioxidant and anti-inflammatory potential of Heritiera fomes ethanolic extract was evaluated.Query Ethanolic extraction of dried and grinded plant leaves and bark was performed. Antioxidant activity was evaluated by 1, 1-diphenyl-2-picryl-hydrazil (DPPH) free radical scavenging assay, ferric reducing antioxidant power (FRAP) and β-carotene bleaching inhibition assay. Total phenolic and flavonoid content were also determined. Anti-inflammatory activity was evaluated by in vitro human RBC membrane stabilizing assay and in vivo mice paw edema test. Ethanolic bark and leaf extracts of H. fomes (S1 and S2, respectively) showed significant scavenging effect on DPPH scavenging 68.51 µg/ml for S1 and 108.64 µg/ml for S2. Potent reduction potential (FRAP) was observed in S1 (83.75 mM of ferrous equivalent) while the FRAP of standard ascorbic acid was found 86.66 μM of ferrous equivalent. S1 also exhibited 87.43% β-carotene bleaching inhibition as comparable to standard butylated hydroxytoluene with 98.85% inhibition. Substantial phenolic and flavonoid content was also observed in S1 (136.83 μg gallic acid equivalent/g of extract and 76.67 μg quercetin equivalent/g of extract, respectively). S1 could provide 61.73% and 53.27% protection of RBC membrane in hypotonicity and heat induced lysis of RBC, respectively while the standard diclofenac sodium provided 91.66% and 86.66% protection, respectively. Furthermore, 44.16% reduction in mice paw edema at 40 μg/kg bw concentration was observed in S1 as compared to standard indomethacine (53.54% in 10 μg/kg bw concentration). The results revealed that the bark extract (S1) of H. fomes possesses significant antioxidant and anti-inflammatory activity.

Molecular Characterization of Selected Local and Exotic Cattle Using RAPD Marker

Khatun, M. Mahfuza,Hossain, Khondoker Moazzem,Rahman, S.M. Mahbubur Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.6

In order to develop specific genetic markers and determine the genetic diversity of Bangladeshi native cattle (Pabna, Red Chittagong) and exotic breeds (Sahiwal), randomly amplified polymorphic DNA (RAPD) analysis was performed using 12 primers. Genomic DNA was extracted from 20 cattle (local and exotic) blood samples and extracted DNA was observed by gel electrophoresis. Among the random primers three were matched and found to be polymorphic. Genetic relations between cattle's were determined by RAPD polymorphisms from a total of 66.67%. Statistical analysis of the data, estimating the genetic distances between cattle and sketching the cluster trees were estimated by using MEGA 5.05 software. Comparatively highest genetic distance (0.834) was found between RCC-82 and SL-623. The lowest genetic distance (0.031) was observed between M-1222 and M-5730. The genetic diversity of Red Chittagong and Sahiwal cattle was relatively higher for a prescribed breed. Adequate diversity in performance and adaptability can be exploited from the study results for actual improvement accruing to conservation and development of indigenous cattle resources.

Antioxidant and antibacterial activity of three herbs belonging to Zingiber genus of Bangladesh

Afiya Aunjum,Rana Biswas,Tauhidur Rahman Nurunnabi,S.M. Mahbubur Rahman,Md. Morsaline Billah,Md. Emdadul Islam,Kazi Mohammed Didarul Islam 경희대학교 융합한의과학연구소 2020 Oriental Pharmacy and Experimental Medicine Vol.20 No.3

Apart from boosting flavor, herbs and spices are also known for their preservative, antioxidant, antimicrobial and other medicinal properties. Bioactive metabolites with aspiring prospect have guided the present study to screen for antibacterial and antioxidant activity of three Zingiber plants available in Bangladesh. For that purpose, rhizomes of Zingiber officinale, Z. montanum and Z. zerumbet were extracted with ethanol as solvent system for bioactivity analysis. Antioxidant activity was assessed by employing DPPH free radical scavenging assay, reducing power assay and ferric reducing antioxidant power (FRAP) assay. Z. officinale extract demonstrated the highest free radical scavenging activity ( IC50: 43.65 μg/ml), reducing power ( EC50: 189.026 μg/ml) and FRAP value (137.18 ± 0.116 μM Fe(II)/100 μg dry weight of extract). Moreover, total phenolic, flavonoid and tannin content were determined which significantly correlated (Pearson correlation) with the antioxidant activities. For the evaluation of antibacterial activity, zone of inhibition (ZOI) was measured by disc diffusion bioassay against four Gram positive bacteria Bacillus megaterium, Staphylococcus aureus, Micrococcus spp., and S. epidermidis as well as ten Gram negative bacteria Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, S. paratyphi, Vibrio cholera, Shigella dysenteriae, S. flexneri, Proteus spp., Mycobacterium spp., and Campylobacter spp. Z. montanum extract exhibited significant activity against E. coli (13.67 ± 0.577 mm ZOI) and Micrococcus spp. (14.33 ± 0.763 mm ZOI) if compared to standard erythromycin with ZOI 15.33 ± 0.577 mm and 15.67 ± 0.577 mm, respectively. Furthermore, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Z. montanum extract were determined against B. megaterium, Micrococcus spp., E. coli and S. paratyphi. Thus, Z. officinale and Z. montanum extracts could be utilized as source and template for the synthesis of potential drugs and food supplements as well.

Antimicrobial activity of endophytic fungi isolated from the mangrove plant Sonneratia apetala (Buch.-Ham) from the Sundarbans mangrove forest

Tauhidur R. Nurunnabi,Farah Sabrin,Dilara I. Sharif,Lutfun Nahar,Md. H. Sohrab,Satyajit D. Sarker,S.M. Mahbubur Rahman,Md. Morsaline Billah 경희대학교 융합한의과학연구소 2020 Oriental Pharmacy and Experimental Medicine Vol.20 No.3

Endophytic fungi reside in the intercellular space of plant nourished by the plant. In return, they provide bioactive molecules which can play critical roles on plant defense system. Fifty six endophytes were isolated from the leaves, root, bark and fruits of Sonneratia apetala, a pioneer mangrove plant in the Sundarbans, Bangladesh. A total of 56 isolates were obtained and 12 different species within 8 genera were identified using morphological and molecular characteristics. Antimicrobial activity of ethyl acetate (EtOAc) and methanolic (MeOH) extracts of these 12 different species were analyzed by resazurin assay and the minimum inhibitory concentrations (MICs) were determined. The fungal extracts showed antimicrobial activities against more than one tested bacterium or fungus among 5 human pathogenic microbes, i.e. Escherichia coli NCTC 12241, Staphylococcus aureus NCTC 12981, Micrococcus lutus NCTC 7508, Pseudomonas aeruginosa NCTC 7508 and Candida albicans ATCC 90028. Overall, methanolic extracts showed greater activity than that of ethyl acetate extracts. Of the isolates identified, Colletotrichum gloeosporioides, Aspergillus niger and Fusarium equiseti were the most active isolates and showed activity against microorganisms under investigation. Methanolic extracts of C. gloeosporioides and A. niger showed the lowest MIC (0.0024 mg/mL) against P. aeruginosa. The study indicates that endophytic fungi isolated from S. apetala species possess potential antimicrobial properties, which could be further investigated.

Assessing Students' Satisfaction in Public Universities in Bangladesh: An Empirical Study

RAHMAN, S.M. Mahbubur,MIA, Md. Shahin,AHMED, Ferdoushi,THONGRAK, Sutonya,KIATPATHOMCHAI, Sirirat Korea Distribution Science Association 2020 The Journal of Asian Finance, Economics and Busine Vol.7 No.8

This study aims to examine the level of students' satisfaction toward the services (i.e. accommodation facilities, transportation facilities, and recreation and sports facilities) provided by the public universities in Bangladesh. The study also aims to identify the major service factors that influence students' satisfaction in public universities in the country. A cross-sectional survey was conducted at six public universities to obtain primary data. A standardized questionnaire was distributed to a total of 500 randomly selected students to collect the data. Several statistical tools, namely, reliability analysis, descriptive statistics, correlation analysis and multiple regression analysis were employed to analyze the data. The findings revealed that recreation and sports facilities have the strongest impact on students' satisfaction in the public universities in Bangladesh. Transportation facilities also have positive and significant impact on student's satisfaction. However, the study found a negative correlation between accommodation facilities and students' satisfaction indicating that students are not satisfied with the accommodation facilities provided by the public universities. The findings of this study provide an insight about students' satisfaction that might be useful to authorities of the public universities and other higher educational institutions in designing policies for various services and facilities to be provided to their students.