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RISS 인기검색어
- 검색키워드
- 저자 : Tauhidur Rahman Nurunnabi (검색결과 2 건)
- 무료
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Tauhidur R. Nurunnabi,Farah Sabrin,Dilara I. Sharif,Lutfun Nahar,Md. H. Sohrab,Satyajit D. Sarker,S.M. Mahbubur Rahman,Md. Morsaline Billah 경희대학교 융합한의과학연구소 2020 Oriental Pharmacy and Experimental Medicine Vol.20 No.3
Endophytic fungi reside in the intercellular space of plant nourished by the plant. In return, they provide bioactive molecules which can play critical roles on plant defense system. Fifty six endophytes were isolated from the leaves, root, bark and fruits of Sonneratia apetala, a pioneer mangrove plant in the Sundarbans, Bangladesh. A total of 56 isolates were obtained and 12 different species within 8 genera were identified using morphological and molecular characteristics. Antimicrobial activity of ethyl acetate (EtOAc) and methanolic (MeOH) extracts of these 12 different species were analyzed by resazurin assay and the minimum inhibitory concentrations (MICs) were determined. The fungal extracts showed antimicrobial activities against more than one tested bacterium or fungus among 5 human pathogenic microbes, i.e. Escherichia coli NCTC 12241, Staphylococcus aureus NCTC 12981, Micrococcus lutus NCTC 7508, Pseudomonas aeruginosa NCTC 7508 and Candida albicans ATCC 90028. Overall, methanolic extracts showed greater activity than that of ethyl acetate extracts. Of the isolates identified, Colletotrichum gloeosporioides, Aspergillus niger and Fusarium equiseti were the most active isolates and showed activity against microorganisms under investigation. Methanolic extracts of C. gloeosporioides and A. niger showed the lowest MIC (0.0024 mg/mL) against P. aeruginosa. The study indicates that endophytic fungi isolated from S. apetala species possess potential antimicrobial properties, which could be further investigated.
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Antioxidant and antibacterial activity of three herbs belonging to Zingiber genus of Bangladesh
Afiya Aunjum,Rana Biswas,Tauhidur Rahman Nurunnabi,S.M. Mahbubur Rahman,Md. Morsaline Billah,Md. Emdadul Islam,Kazi Mohammed Didarul Islam 경희대학교 융합한의과학연구소 2020 Oriental Pharmacy and Experimental Medicine Vol.20 No.3
Apart from boosting flavor, herbs and spices are also known for their preservative, antioxidant, antimicrobial and other medicinal properties. Bioactive metabolites with aspiring prospect have guided the present study to screen for antibacterial and antioxidant activity of three Zingiber plants available in Bangladesh. For that purpose, rhizomes of Zingiber officinale, Z. montanum and Z. zerumbet were extracted with ethanol as solvent system for bioactivity analysis. Antioxidant activity was assessed by employing DPPH free radical scavenging assay, reducing power assay and ferric reducing antioxidant power (FRAP) assay. Z. officinale extract demonstrated the highest free radical scavenging activity ( IC50: 43.65 μg/ml), reducing power ( EC50: 189.026 μg/ml) and FRAP value (137.18 ± 0.116 μM Fe(II)/100 μg dry weight of extract). Moreover, total phenolic, flavonoid and tannin content were determined which significantly correlated (Pearson correlation) with the antioxidant activities. For the evaluation of antibacterial activity, zone of inhibition (ZOI) was measured by disc diffusion bioassay against four Gram positive bacteria Bacillus megaterium, Staphylococcus aureus, Micrococcus spp., and S. epidermidis as well as ten Gram negative bacteria Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, S. paratyphi, Vibrio cholera, Shigella dysenteriae, S. flexneri, Proteus spp., Mycobacterium spp., and Campylobacter spp. Z. montanum extract exhibited significant activity against E. coli (13.67 ± 0.577 mm ZOI) and Micrococcus spp. (14.33 ± 0.763 mm ZOI) if compared to standard erythromycin with ZOI 15.33 ± 0.577 mm and 15.67 ± 0.577 mm, respectively. Furthermore, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Z. montanum extract were determined against B. megaterium, Micrococcus spp., E. coli and S. paratyphi. Thus, Z. officinale and Z. montanum extracts could be utilized as source and template for the synthesis of potential drugs and food supplements as well.
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