Augmenter of Liver Regeneration Alleviates Renal Hypoxia-Reoxygenation Injury by Regulating Mitochondrial Dynamics in Renal Tubular Epithelial Cells

Long, Rui-ting,Peng, Jun-bo,Huang, Li-li,Jiang, Gui-ping,Liao, Yue-juan,Sun, Hang,Hu, Yu-dong,Liao, Xiao-hui Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.12

Mitochondria are highly dynamic organelles that constantly undergo fission and fusion processes that closely related to their function. Disruption of mitochondrial dynamics has been demonstrated in acute kidney injury (AKI), which could eventually result in cell injury and death. Previously, we reported that augmenter of liver regeneration (ALR) alleviates renal tubular epithelial cell injury. Here, we gained further insights into whether the renoprotective roles of ALR are associated with mitochondrial dynamics. Changes in mitochondrial dynamics were examined in experimental models of renal ischemia-reperfusion (IR). In a model of hypoxia-reoxygenation (HR) injury in vitro, dynamin-related protein 1 (Drp1) and mitochondrial fission process protein 1 (MTFP1), two key proteins of mitochondrial fission, were downregulated in the Lv-ALR + HR group. ALR overexpression additionally had an impact on phosphorylation of Drp1 Ser637 during AKI. The inner membrane fusion protein, Optic Atrophy 1 (OPA1), was significantly increased whereas levels of outer membrane fusion proteins Mitofusin-1 and -2 (Mfn1, Mfn2) were not affected in the Lv-ALR + HR group, compared with the control group. Furthermore, the mTOR/4E-BP1 signaling pathway was highly activated in the Lv-ALR + HR group. ALR overexpression led to suppression of HR-induced apoptosis. Our collective findings indicate that ALR gene transfection alleviates mitochondrial injury, possibly through inhibiting fission and promoting fusion of the mitochondrial inner membrane, both of which contribute to reduction of HK-2 cell apoptosis. Additionally, fission processes are potentially mediated by promoting tubular cell survival through activating the mTOR/4E-BP1 signaling pathway.

Normal Estimation for Mass Point Clouds of Irregular Model in the 3D Reconstruction based on Fuzzy Inference

Liu Yan-ju,Jiang Jin-gang,Miao Feng-juan,Tao Bai-rui,Zhang Hong-lie 보안공학연구지원센터 2014 International Journal of Grid and Distributed Comp Vol.7 No.5

This paper presents a fuzzy normal estimate for mass point clouds of irregular models in reconstruction. The irregular model is complex object that some part is smooth and some parts are irregular including sharp features. Therefore, we put kNN and curvature of mass point clouds to fuzzy inference system to divide the kind of point clouds and the output of FIS can determine which part of tooth point clouds belong to. For different kinds point clouds, corresponding algorithm is given. Point clouds in the smooth area are estimated normal by PCA directly and ones in other regions of thin or sharp area are estimated by checker and attach points. This method is simpler than those complex methods used on the whole point clouds directly. The experiment results show that much time is saved and surface reconstruction is very fine than PCA and WLOP.

Augmenter of Liver Regeneration Alleviates Renal Hypoxia-Reoxygenation Injury by Regulating Mitochondrial Dynamics in Renal Tubular Epithelial Cells

Xiao-hui Liao,Rui-ting Long,Jun-bo Peng,Li-li Huang,Gui-ping Jiang,Yue-juan Liao,Hang Sun,Yu-dong Hu 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.12

Mitochondria are highly dynamic organelles that constantly undergo fission and fusion processes that closely related to their function. Disruption of mitochondrial dynamics has been demonstrated in acute kidney injury (AKI), which could eventually result in cell injury and death. Previously, we reported that augmenter of liver regeneration (ALR) alleviates renal tubular epithelial cell injury. Here, we gained further insights into whether the renoprotective roles of ALR are associated with mitochondrial dynamics. Changes in mitochondrial dynamics were examined in experimental models of renal ischemia-reperfusion (IR). In a model of hypoxia-reoxygenation (HR) injury in vitro, dynamin-related protein 1 (Drp1) and mitochondrial fission process protein 1 (MTFP1), two key proteins of mitochondrial fission, were downregulated in the Lv-ALR + HR group. ALR overexpression additionally had an impact on phosphorylation of Drp1 Ser637 during AKI. The inner membrane fusion protein, Optic Atrophy 1 (OPA1), was significantly increased whereas levels of outer membrane fusion proteins Mitofusin-1 and -2 (Mfn1, Mfn2) were not affected in the Lv-ALR + HR group, compared with the control group. Furthermore, the mTOR/4E-BP1 signaling pathway was highly activated in the Lv-ALR + HR group. ALR overexpression led to suppression of HR-induced apoptosis. Our collective findings indicate that ALR gene transfection alleviates mitochondrial injury, possibly through inhibiting fission and promoting fusion of the mitochondrial inner membrane, both of which contribute to reduction of HK-2 cell apoptosis. Additionally, fission processes are potentially mediated by promoting tubular cell survival through activating the mTOR/4E-BP1 signaling pathway.

Controlled Synthesis of Hydroxyapatite Using a Novel Natural Rosin-Based Surfactant

Shu-Hui Zhan,Xue-He Jiang,Juan Li,Zhi Meng,Lin-Lin Chen,Chun-Rui Han 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.8

Hydroxyapatite (HAP) with multiform morphologies, such as hollow dandelion-like bundles and nanoparticles with a diameter of 50 nm, was prepared using natural rosin-based surfactant dehydroabietyl phosphate diester (DDPD) as phosphorus source, crystal growth control agent, and template simultaneously by a facile hydrothermal method. Samples were obtained and characterized by X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). Results showed that DDPD and pH value of solution were the key factors for the morphology of HAP. Hollow dandelion-like bundles HAP, containing the Ca-monodehydroabietyl phosphate (PM-Ca) organic metal compounds, were formed at pH=3 without acid-base regulation, and nanoparticles were obtained at pH=12. SEM exhibited that the hollow dandelion-like bundles HAP are of 10 μm (outer) and 1 μm (inner) diameter, respectively. Cell viabilities are above 95% when the cells are co-cultured with all HAP samples at concentrations in the range of 250–1000 μg/ml. It indicated that the prepared HAP with PM-Ca has a good cytocompatibility without apparent toxicity. Finally, the possible formation mechanism of the HAP microstructures was discussed in detail.

Toxic Epidermal Necrolysis Induced by Sintilimab: A Case Report

Ya-lei Lye,Bin Shan,Chen-hong Jia,Jiang Liu,Juan Hou,Wen-li Du,Rui Feng,Ping Liang 대한피부과학회 2023 Annals of Dermatology Vol.35 No.-

Sintilimab is an anti-programmed cell death receptor-1 antibody. The phase III clinical trial ORIENT-12 confirmed the safety of sintilimab combined with pemetrexed/platinum in the treatment of advanced squamous non-small cell lung cancer. Skin reactions are the most commonly reported adverse events of immune checkpoint inhibitors and are rarely severe. We describe a case of toxic epidermal necrolysis related to sintilimab in an elderly oncologic patient. 3 weeks after immunotherapy, the patient developed an extensive rash and diffuse itching, rapidly evolving into macules, blisters, bullae and erosions. Causal evaluation was performed based on the algorithm of drug causality for epidermal necrolysis and national Food and Drug Administration qualitative analysis. The patient responded to high-dose glucocorticosteroid and supportive therapy, alongside with local wound care. If immune checkpoint inhibitors need to be extrapolated clinically, strictly following evidence-based research, promptly detecting and treating adverse reactions is crucial.

Prestressed concrete bridges with corrugated steel webs: Nonlinear analysis and experimental investigation

Xia-chun Chen,Zhi-zhou Bai,Yu Zeng,Rui-juan Jiang,Francis T.K. Au 국제구조공학회 2016 Steel and Composite Structures, An International J Vol.21 No.5

Concrete bridges with corrugated steel webs and prestressed by both internal and external tendons have emerged as one of the promising bridge forms. In view of the different behaviour of components and the large shear deformation of webs with negligible flexural stiffness, the assumption that plane sections remain plane may no longer be valid, and therefore the classical Euler-Bernoulli and Timoshenko beam models may not be applicable. In the design of this type of bridges, both the ultimate load and ductility should be examined, which requires the estimation of full-range behaviour. An analytical sandwich beam model and its corresponding beam finite element model for geometric and material nonlinear analysis are developed for this type of bridges considering the diaphragm effects. Different rotations are assigned to the flanges and corrugated steel webs to describe the displacements. The model accounts for the interaction between the axial and flexural deformations of the beam, and uses the actual stress-strain curves of materials considering their stress path-dependence. With a nonlinear kinematical theory, complete description of the nonlinear interaction between the external tendons and the beam is obtained. The numerical model proposed is verified by experiments.