cAMP induction by ouabain promotes endothelin-1 secretion via MAPK/ERK signaling in beating rabbit atria

Li-qun Peng,Ping Li,Qiu-li Zhang,Lan Hong,Li-ping Liu,Xun Cui,Bai-ri Cui 대한생리학회-대한약리학회 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.1

Adenosine 3 ,5 -cyclic monophosphate (cAMP) participates in the regulation of numerous cellular functions, including the Na⁺-K⁺-ATPase (sodium pump). Ouabain, used in the treatment of several heart diseases, is known to increase cAMP levels but its effects on the atrium are not understood. The aim of the present study was to examine the effect of ouabain on the regulation of atrial cAMP production and its roles in atrial endothelin-1 (ET-1) secretion in isolated perfused beating rabbit atria. Our results showed that ouabain (3.0 μmol/L) significantly increased atrial dynamics and cAMP levels during recovery period. The ouabainincreased atrial dynamics was blocked by KB-R7943 (3.0 μmol/L), an inhibitor for reverse mode of Na⁺-Ca²⁺ exchangers (NCX), but did not by L-type Ca²⁺ channel blocker nifedipine (1.0 μmol/L) or protein kinase A (PKA) selective inhibitor H-89 (3.0 μmol/L). Ouabain also enhanced atrial intracellular cAMP production in response to forskolin and theophyline (100.0 μmol/L), an inhibitor of phosphodiesterase, potentiated the ouabain-induced increase in cAMP. Ouabain and 8-Bromo-cAMP (0.5 μmol/L) markedly increased atrial ET-1 secretion, which was blocked by H-89 and by PD98059 (30 μmol/L), an inhibitor of extracellular-signal-regulated kinase (ERK) without changing ouabain-induced atrial dynamics. Our results demonstrated that ouabain increases atrial cAMP levels and promotes atrial ET-1 secretion via the mitogen-activated protein kinase (MAPK)/ERK signaling pathway. These findings may explain the development of cardiac hypertrophy in response to digitalis-like compounds.

β-elemene Induces Caspase-dependent Apoptosis in Human Glioma Cells in vitro through the Upregulation of Bax and Fas/FasL and Downregulation of Bcl-2

Li, Chen-Long,Chang, Liang,Guo, Lin,Zhao, Dan,Liu, Hui-Bin,Wang, Qiu-Shi,Zhang, Ping,Du, Wen-Zhong,Liu, Xing,Zhang, Hai-Tao,Liu, Yang,Zhang, Yao,Xie, Jing-Hong,Ming, Jian-Guang,Cui, Yu-Qiong,Sun, Ying Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Background: ${\beta}$-elemene, extracted from herb medicine Curcuma wenyujin has potent anti-tumor effects in various cancer cell lines. However, the activity of ${\beta}$-elemene against glioma cells remains unclear. In the present study, we assessed effects of ${\beta}$-elemene on human glioma cells and explored the underlying mechanism. Materials and Methods: Human glioma U87 cells were used. Cell proliferation was determined with MTT assay and colony formation assay to detect the effect of ${\beta}$-elemene at different doses and times. Fluorescence microscopy was used to observe cell apoptosis with Hoechst 33258 staining and change of glioma apoptosis and cell cycling were analyzed by flow cytometry. Real-time quantitative PCR and Western-blotting assay were performed to investigated the influence of ${\beta}$-elemene on expression levels of Fas/FasL, caspase-3, Bcl-2 and Bax. The experiment was divided into two groups: the blank control group and ${\beta}$-elemne treatment group. Results: With increase in the concentration of ${\beta}$-elemene, cytotoxic effects were enhanced in the glioma cell line and the concentration of inhibited cell viability ($IC_{50}$) was $48.5{\mu}g/mL$ for 24h. ${\beta}$-elemene could induce cell cycle arrest in the G0/G1 phase. With Hoechst 33258 staining, apoptotic nuclear morphological changes were observed. Activation of caspase-3,-8 and -9 was increased and the pro-apoptotic factors Fas/FasL and Bax were upregulated, while the anti-apoptotic Bcl-2 was downregulated after treatment with ${\beta}$-elemene at both mRNA and protein levels. Furthermore, proliferation and colony formation by U87 cells were inhibited by ${\beta}$-elemene in a time and does-dependent manner. Conclusions: Our results indicate that ${\beta}$-elemene inhibits growth and induces apoptosis of human glioma cells in vitro. The induction of apoptosis appears to be related with the upregulation of Fas/FasL and Bax, activation of caspase-3,-8 and -9 and downregulation of Bcl-2, which then trigger major apoptotic cascades.

Superdense coding using a non-symmetric multipartite quantum channel

Qiu-Bo Fan,Shou Zhang,엄정인,Feng-Yue LI,연규황,Li-li Sun,Zhe JIN 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.46 No.4

We investigate superdense coding in the case of non-symmetric Hilbert spaces of the sender’s and the receiver’s particles sharing the multipartite maximally quantum channel. We also consider that a symmetric quantum channel is transformed into a non-symmetric one. As a result, the efficiency of information transmission is successfully increased.

Electrochemical Polymerization of Methoxy Polyethylene Glycol Acrylate on Carbon Fiber for Improved Interfacial Properties

Qiu Li,Ai-ling Zhang,Meng Zhang,Fang-fang Li,Song Wang,San-xi Li,Yong-jiang Li,Ze-fei Sun 한국섬유공학회 2019 Fibers and polymers Vol.20 No.12

Methoxy polyethylene glycol acrylate (MPGA) was electrochemical polymerized on carbon fiber (CF) by usingcyclic voltammetry (CV) to enhance the surface properties of CF. Fourier transform infrared spectroscopy (FTIR), scanningelectron microscopy (SEM) and thermogravimetry analysis (TGA) investigations confirmed that MPGA electropolymerizedon CF (MPGA-CF) was obtained. The low frequency capacitance of MPGA-CF (CLF=5.85 mF·cm-2) was higher thanCF(CLF=4.75 mF·cm-2) based on the result of electrochemical impedance spectroscopy(EIS) and the data obtained fitted withan R(Q(R(Q(R(CR))))) equivalent circuit model. Contact angle experiment showed that the contact angle between water andMPGA-CF is significantly lower than that between water and CF. Mechanical experimental results indicated that theinterlaminar shear strength (ILSS) of MPGA-CF/epoxy resin composites (32.24 MPa) has improved by 134.81 % comparewith the CF/epoxy resin composites (13.73 MPa), which significantly enhanced interfacial properties of epoxy resincomposites.

Electro-optic Coefficient Measurement of the Double Electro-Optic Molecules Polymer based on Terahertz Application

Zhang Ying,Qiu Chengjun,Li You,Zhang Wenlong,Wang Xuan 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.10

In the terahertz (THz) science research field, electro-optic (EO) polymer possesses many superior characters compared with mineral crystal. Two kinds of the side group EO polymer, polyphosphazene with plmaol grained by 2, 4-dinitroaniline and polyphenylacrylate containing p-nitro bisazo group, are synthesized and characterized. For higher EO coefficient, the double EO molecules polymer thin films are prepared by mixing a small nonlinear optical (NLO) molecule into the side group EO polymer. The principles of preparation are given and the EO coefficients are tested. The effect on the EO coefficients by different mixing proportion and different poling temperature are analyzed in details. Experiments results show that the double EO molecules polymer has fine stability, higher EO coefficient and can be easily processed. This kind of material has the potential to be widely applied in the emission and detection of THz radiation.

New Insights Into Refractory Chronic Cough and Unexplained Chronic Cough: A 6-Year Ambispective Cohort Study

Zhang Mengru,Morice Alyn H.,Si Fengli,Zhang Li,Chen Qiang,Wang Shengyuan,Zhu Yiqing,Xu Xianghuai,Yu Li,Qiu Zhongmin 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.6

Purpose: Only limited studies have depicted the unique features and management of refractory chronic cough (RCC) and unexplained chronic cough (UCC). These led to the initiation of this study, which reported the demographic characteristics, manifestations, and long-term outcomes on a large series of consecutive RCC/UCC patients, providing a guideline-led real-world clinical experience. Methods: Retrospective baseline information was obtained from Clinical Research Database (January 2016 to May 2021). At least 6 months after the last clinic visit, included subjects were prospectively followed up. Results: Three hundred and sixty-nine RCC and UCC patients (199 females, 53.9%) were analyzed. The median cough duration was 24.0 (12.0–72.0) months. Laryngeal symptoms were reported in 95.9% of the patients. The common triggers for coughing were talking (74.9%), pungent odors (47.3%), eating (45.5%), and cold air (42.8%). RCC was considered in 38.2%, and the remainder of 228 patients had UCC, with an equal sex distribution (P = 0.66). Among the 141 RCCs, 90.8% (128) had refractory reflux cough, which was more responsive to current treatments (P < 0.01). Although most features and test results between RCC and UCC were similar, UCC was more commonly inappropriately treated (P < 0.01). Nineteen (7.7–41.1) months after the final clinic visit, 31.2% still coughed persistently, while 68.8% reported cough improvement or remission. RCC reported more favorable treatment outcomes (including cough improvement, control, and spontaneous remission) than UCC (P < 0.01). Coughs with long duration before the initial cough clinic visit (P < 0.01), frequent urinary incontinence (P < 0.01), and being sensitive to “talking” (P < 0.01) or “cold air” (P < 0.01) were less likely to be solved. Conclusions: The current treatments only improve cough symptoms in two-thirds of patients. Clinical indicators for treatment failure were those coughing for long duration and being sensitive to “talking” or “cold air.”

Dirac semimetal-enabled multi-bit coding metasurface for dynamic manipulation of terahertz beams

Zhang Yonggang,Yin Kehao,Liang Lanju,Yao Haiyun,Yan Xin,Hu Xiaofei,Huang Chengcheng,Qiu Fu,Zhang Rui,Li Yuanping,Wang Yaru,Li Zhenhua,Wang Ziqun 한국물리학회 2024 Current Applied Physics Vol.58 No.-

In this study, a switchable multi-bit coding metasurface that is applied under a terahertz (THz) frequency by adjusting the Fermi level (EF) of Dirac semimetals (DSMs) is proposed. At a EF of 0.2 eV, a 1-bit coding metasurface can be applied in the 2.58–2.62 THz. At 0.3 eV, a 3-bit coding metasurface is realized at 1.88 THz, and at 0.05 eV, the phase of the coding units coincides in the 1.5–3 THz. So, different functions of the metasurface can be realized. The proposed coding metasurfaces has promising applications in terahertz communication.

SUBCULTURAL DIFFERENCES OF SERVICE FAILURE RESPONSE: GRID-GROUP ANALYSIS

Yi Zhang,Mimi Li,Shangzhi Qiu 글로벌지식마케팅경영학회 2016 Global Marketing Conference Vol.2016 No.7

High brightness and precise adjustment of multicolor-tunable luminescence of Lu2GeO5:Tb3+, Eu3+ phosphors for white LEDs

Shaoan Zhang,Yuanxing Li,Wenfeng Li,Zhenzhang Li,Minfan Qiu,Zhongfei Mu,Yang Li,Yihua Hu 한국물리학회 2019 Current Applied Physics Vol.19 No.9

High brightness and precise adjustment of luminescence colour of phosphors are two main targets in the research of phosphor-converted white LEDs. However, few feasible strategy can be employed to achieve the multicolortunable luminescence under the premise of maintaining high quantum efficiency. Here, we demonstrate a highefficiency energy-transfer process from Tb3+ to Eu3+ ions with a higher luminescent quantum efficiency (64.5% and 53.4%, respectively), and green-red multicolor emission in Lu2GeO5 host via varying the doping content of Tb3+ and Eu3+ ions. Besides, Lu2GeO5:Tb3+, Lu2GeO5: Eu3+ and Lu2GeO5: Tb3+, Eu3+ all exhibit weak thermal quenching which ensures the stable use of white LED device in the high temperature environment. This paper provides a novel multicolor-tunable phosphor with high brightness, efficient energy transfer and weak thermal quenching, which presents a potential application for UV-converted white LEDs.

cAMP induction by ouabain promotes endothelin-1 secretion via MAPK/ERK signaling in beating rabbit atria

Peng, Li-qun,Li, Ping,Zhang, Qiu-li,Hong, Lan,Liu, Li-ping,Cui, Xun,Cui, Bai-ri The Korean Society of Pharmacology 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.1

Adenosine 3',5'-cyclic monophosphate (cAMP) participates in the regulation of numerous cellular functions, including the $Na^+-K^+$-ATPase (sodium pump). Ouabain, used in the treatment of several heart diseases, is known to increase cAMP levels but its effects on the atrium are not understood. The aim of the present study was to examine the effect of ouabain on the regulation of atrial cAMP production and its roles in atrial endothelin-1 (ET-1) secretion in isolated perfused beating rabbit atria. Our results showed that ouabain ($3.0{\mu}mol/L$) significantly increased atrial dynamics and cAMP levels during recovery period. The ouabain-increased atrial dynamics was blocked by KB-R7943 ($3.0{\mu}mol/L$), an inhibitor for reverse mode of $Na^+-Ca^{2+}$ exchangers (NCX), but did not by L-type $Ca^{2+}$ channel blocker nifedipine ($1.0{\mu}mol/L$) or protein kinase A (PKA) selective inhibitor H-89 ($3.0{\mu}mol/L$). Ouabain also enhanced atrial intracellular cAMP production in response to forskolin and theophyline ($100.0{\mu}mol/L$), an inhibitor of phosphodiesterase, potentiated the ouabain-induced increase in cAMP. Ouabain and 8-Bromo-cAMP ($0.5{\mu}mol/L$) markedly increased atrial ET-1 secretion, which was blocked by H-89 and by PD98059 ($30{\mu}mol/L$), an inhibitor of extracellular-signal-regulated kinase (ERK) without changing ouabain-induced atrial dynamics. Our results demonstrated that ouabain increases atrial cAMP levels and promotes atrial ET-1 secretion via the mitogen-activated protein kinase (MAPK)/ERK signaling pathway. These findings may explain the development of cardiac hypertrophy in response to digitalis-like compounds.