Molecular cloning and expression analysis of a myosin light chain 1 (MLC‐1) gene from Indian meal moth Plodia interpunctella (Lepidoptera: Pyralidae)

Qiu‐Ning Liu,Dan-Dan Bian,Chun-Lin Zhou,Bo-Ping Tang 한국곤충학회 2015 Entomological Research Vol.45 No.6

Myosin light chain 1 (MLC‐1) protein acts in the organization, dynamics and transport processes associated with the cytoskeleton. In this work, an MLC‐1 gene was cloned and characterized from the Indian meal moth, Plodia interpunctella (Lepidoptera: Pyralidae). The isolated PiMLC‐1 cDNA is 913 bp, including a 5′‐untranslated region (UTR) of 79 bp, 3′‐UTR of 381 bp and an open reading frame (ORF) of 453 bp encoding a polypeptide of 150 amino acids, which contains two calcium binding domains (EF‐hands). The deduced PiMLC‐1 protein sequence has 39–94% comparison with other individuals. The qPCR analysis revealed that PiMLC‐1 was expressed in the four developmental stages (egg, larva, pupa and adult) and in all tissues tested, suggesting that it plays an important role in development of P. interpunctella. Based on the MLC‐1 amino acids, phylogenetic analysis showed a similar topology with the traditional classification, suggesting the potential value of the MLC‐1 protein in phylogenetic inference.

Combination of Tumor Volume and Epstein-Barr Virus DNA Improved Prognostic Stratification of Stage II Nasopharyngeal Carcinoma in the Intensity Modulated Radiotherapy Era: A Large-Scale Cohort Study

Qiu-Yan Chen,Shao-Yan Guo,Lin-Quan Tang,Tong-Yu Lu,Bo-Lin Chen,Qi-Yu Zhong,Meng-Sha Zou,Qing-Nan Tang,Wen-Hui Chen,Shan-Shan Guo,Li-Ting Liu,Yang Li,Ling Guo,Hao-Yuan Mo,Rui Sun,Dong-Hua Luo,Chong Zha 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3

Purpose Little is known about combination of the circulating Epstein-Barr viral (EBV) DNA and tumor volume in prognosis of stage II nasopharyngeal carcinoma (NPC) patients in the intensity modulated radiotherapy (IMRT) era. We conducted this cohort study to evaluate the prognostic values of combining these two factors. Materials and Methods By Kaplan-Meier, we compare the differences of survival curves between 385 patients with different EBV DNA or tumor volume levels, or with the combination of two biomarkers mentioned above. Results Gross tumor volume of cervical lymph nodes (GTVnd, p < 0.001) and total tumor volume (GTVtotal, p < 0.001) were both closely related to pretreatment EBV DNA, while gross tumor volume of nasopharynx (GTVnx, p=0.047) was weakly related to EBV DNA. EBV DNA was significantly correlated with progress-free survival (PFS, p=0.005), locoregional-free survival (LRFS, p=0.039), and distant metastasis-free survival (DMFS, p=0.017), while GTVtotal, regardless of GTVnx and GTVnd, had a significant correlation with PFS and LRFS. The p-values of GTVtotal for PFS and LRFS were 0.008 and 0.001, respectively. According to GTVtotal and pretreatment EBV DNA level, patients were divided into a low-risk group (EBV DNA 0 copy/mL, GTVtotal < 30 cm3; EBV DNA 0 copy/mL, GTVtotal  30 cm3; or EBV DNA > 0 copy/mL, GTVtotal < 30 cm3) and a high-risk group (EBV DNA > 0 copy/mL, GTVtotal  30 cm3). When patients in the low-risk group were compared with those in the high-risk group, 3-year PFS (p=0.003), LRFS (p=0.010), and DMFS (p=0.031) rates were statistically significant. Conclusion Pretreatment plasma EBV DNA and tumor volume were both closely correlated with prognosis of stage II NPC patients in the IMRT era. Combination of EBV DNA and tumor volume can refine prognosis and indicate for clinical therapy.

Preparation of W–V functionally gradient material by spark plasma sintering

Tang Yi,Qiu Wenbin,Chen Longqing,Yang Xiaoliang,Song Yangyipeng,Tang Jun 한국원자력학회 2020 Nuclear Engineering and Technology Vol.52 No.8

Functionally gradient material (FGM) is promisingly effective in mitigating the thermal stress between plasma facing materials (PFM) and structural materials. However, the corresponding research with respect to W/V FGM has not been reported yet. In this work, we firstly report the successful fabrication of W/V FGM by a combined technology of mechanical alloying (MA) and spark plasma sintering (SPS). The microhardness and microstructure of the consolidated sample were both investigated. W/V stacks show significantly enhanced microhardness (>100%) compared with pure W plate, which is beneficial to the integral strength of the hybrid structure. Furthermore, we clarify that the different ductility of W and V should be carefully considered, otherwise W/V powder might aggregate and lead to the formation of compositional segregation, and simultaneously unmask the impact of V proportion on the distribution of second phase in WeV binary alloy system. This w

Pretreatment Serum Amyloid A and C-reactive Protein Comparing with Epstein-Barr Virus DNA as Prognostic Indicators in Patients with Nasopharyngeal Carcinoma: A Prospective Study

Qiu-Yan Chen,Qing-Nan Tang,Lin-Quan Tang,Wen-Hui Chen,Shan-Shan Guo,Li-Ting Liu,Chao-Feng Li,Yang Li,Yu-Jing Liang,Xue-Song Sun,Ling Guo,Hao-Yuan Mo,Rui Sun,Dong-Hua Luo,Yu-Ying Fan,Yan He,Ming-Yuan C 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3

Purpose The measuring Epstein-Barr virus (EBV) DNA is an important predictor of nasopharyngeal carcinoma (NPC). This study evaluated the predictive value of pretreatment serum amyloid A (SAA) and C-reactive protein (CRP) comparing with EBV DNA in patients with NPC. Materials and Methods In an observational study of 419 non-metastatic NPC patients, we prospectively evaluated the prognostic effects of pretreatment SAA, CRP, and EBV DNA on survival. The primary endpoint was progress-free survival (PFS). Results The median level of SAA and CRP was 4.28 mg/L and 1.88 mg/L, respectively. For the high- SAA group (> 4.28 mg/L) versus the low-SAA ( 4.28 mg/L) group and the high-CRP group (> 1.88 mg/L) versus the low-CRP ( 1.88 mg/L) group, the 5-year PFS was 64.5% versus 73.1% (p=0.013) and 65.2% versus 73.3% (p=0.064), respectively. EBV DNA detection showed a superior predictive result, the 5-year PFS in the EBV DNA  1,500 copies/mL group was obviously different than the EBV DNA < 1,500 copies/mL group (62.2% versus 77.8%, p < 0.001). Multifactorial Cox regression analysis confirmed that in the PFS, the independent prognostic factors were including EBV DNA (hazard ratio [HR], 1.788; p=0.009), tumour stage (HR, 1.903; p=0.021), and node stage (HR, 1.498; p=0.049), but the SAA and CRP were not included in the independent prognostic factors. Conclusion The results of SAA and CRP had a certain relationship with the prognosis of NPC, and the prognosis of patients with high level of SAA and CRP were poor. However, the predictive ability of SAA and CRP was lower than that of EBV DNA.

Optimization of impulse water turbine based on GA-BP neural network arithmetic

Lingdi Tang,Shouqi Yuan,Yue Tang,Zhipeng Qiu 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.1

To develop an optimum design method for impulse water turbines with low specific speed, a representative impulse water turbine with low specific speed used in agricultural irrigation machinery was optimized with a combination of an orthogonal experimental design, a genetic algorithm, and a BP neural network in this study. Numerical calculation was applied to analyze interflow characteristics for optimized and original water turbines. Results showed that the internal flow characteristics of the optimized water turbine presented remarkable improvement compared with the original water turbine. Pressure distribution increased, the vortex strip in the draft tube was reduced remarkably, and impeller torque increased by 26 %. In addition, the optimized impeller was manufactured by 3D printing, and performance comparison was conducted between experiments of the optimized and original water turbines. The efficiency of the optimized water turbine reached 42.5 %, which exceeded the original water turbine’s of 8.5 %. With increasing rotating speed, maximum efficiency running point moved to a high flow rate, and highly efficient areas expanded. Internal characteristic analysis and a full-scale experiment for both water turbines showed that the performance of the optimized water turbine exhibited substantial improvement. The analysis and experiment also verified the theoretical correctness and feasibility of the proposed optimum design method.

Coffin-Siris syndrome in two chinese patients with novel pathogenic variants of ARID1A and SMARCA4

Liu Mingjie,Wan Linlin,Wang Chunrong,Yuan Hongyu,Peng Yun,Wan Na,Tang Zhichao,Yuan Xinrong,Chen Daji,Long Zhe,Shi Yuting,Qiu Rong,Tang Beisha,Tang Beisha,Chen Zhao 한국유전학회 2022 Genes & Genomics Vol.44 No.9

Background: Coffin-Siris syndrome (CSS) is a rare congenital syndrome characterized by developmental delay, intellectual disability, microcephaly, coarse face and hypoplastic nail of the fifth digits. Heterozygous variants of different BAF complex-related genes were reported to cause CSS, including ARID1A and SMARCA4. So far, no CSS patients with ARID1A and SMARCA4 variants have been reported in China. Objective: The aim of the current study was to identify the causes of two Chinese patients with congenital growth deficiency and intellectual disability. Methods: Genomic DNA was extracted from the peripheral venous blood of patients and their family members. Genetic analysis included whole-exome and Sanger sequencing. Pathogenicity assessments of variants were performed according to the guideline of the American College of Medical Genetics and Genomics. The phenotypic characteristics of all CSS subtypes were summarized through literature review. Results: We identified two Chinese CSS patients carrying novel variants of ARID1A and SMARCA4 respectively. The cases presented most core symptoms of CSS except for the digits involvement. Additionally, we performed a review of the phenotypic characteristics in CSS, highlighting phenotypic varieties and related potential causes. Conclusions: We reported the first Chinese CSS2 and CSS4 patients with novel variants of ARID1A and SMARCA4. Our study expanded the genetic and phenotypic spectrum of CSS, providing a comprehensive overview of genotype-phenotype correlations of CSS.

Research on the Influence of Inter-turn Short Circuit Fault on the Temperature Field of Permanent Magnet Synchronous Motor

Qiu, Hongbo,Yu, Wenfei,Tang, Bingxia,Yang, Cunxiang,Zhao, Haiyang The Korean Institute of Electrical Engineers 2017 Journal of Electrical Engineering & Technology Vol.8 No.1

When the inter-turn short circuit (ITSC) fault occurs, the distortion of the magnetic field is serious. The motor loss variations of each part are obvious, and the motor temperature field is also affected. In order to obtain the influence of the ITSC fault on the motor temperature distribution, firstly, the normal and the fault finite element models of the permanent magnet synchronous motor (PMSM) were established. The magnetic density distribution and the eddy current density distribution were analyzed, and the mechanism of loss change was revealed. The effects of different forms and degrees of the fault on the loss were obtained. Based on the loss analysis, the motor temperature field calculation model was established, and the motor temperature change considering the loop current was analyzed. The influence of the fault on the motor temperature distribution was revealed. The sensitivity factors that limit the motor continuous operation were obtained. Finally, the correctness of the simulation was verified by experiments. The conclusions obtained are of great significance for the fault and high temperature demagnetization of the permanent magnet analysis.

Role of stearyl-coenzyme A desaturase 1 in mediating the effects of palmitic acid on endoplasmic reticulum stress, inflammation, and apoptosis in goose primary hepatocytes

Tang, Bincheng,Qiu, Jiamin,Hu, Shenqiang,Li, Liang,Wang, Jiwen Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.7

Objective: Unlike mammals, goose fatty liver shows a strong tolerance to fatty acids without obvious injury. Stearyl-coenzyme A desaturase 1 (SCD1) serves crucial role in desaturation of saturated fatty acids (SAFs), but its role in the SAFs tolerance of goose hepatocytes has not been reported. This study was conducted to explore the role of SCD1 in regulating palmitic acid (PA) tolerance of goose primary hepatocytes. Methods: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide was examined to reflect the effect of PA on hepatocytes viability, and quantitative polymerase chain reaction was used to detect the mRNA levels of several genes related to endoplasmic reticulum (ER) stress, inflammation, and apoptosis, and the role of SCD1 in PA tolerance of goose hepatocytes was explored using RNA interfere. Results: Our results indicated that goose hepatocytes exhibited a higher tolerant capacity to PA than human hepatic cell line (LO2 cells). In goose primary hepatocytes, the mRNA levels of fatty acid desaturation-related genes (SCD1 and fatty acid desaturase 2) and fatty acid elongate enzyme-related gene (elongase of very long chain fatty acids 6) were significantly upregulated with 0.6 mM PA treatment. However, in LO2 cells, expression of ER stress-related genes (x box-binding protein, binding immunoglobulin protein, and activating transcription factor 6), inflammatory response-related genes (interleukin-6 [IL-6], interleukin-1β [IL-1β], and interferon-γ) and apoptosis-related genes (bcl-2-associated X protein, b-cell lymphoma 2, Caspase-3, and Caspase-9) was significantly enhanced with 0.6 mM PA treatment. Additionally, small interfering RNA (siRNA) mediated downregulation of SCD1 significantly reduced the PA tolerance of goose primary hepatocytes under the treatment of 0.6 mM PA; meanwhile, the mRNA levels of inflammatory-related genes (IL-6 and IL-1β) and several key genes involved in the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT), forkhead box O1 (FoxO1), mammalian target of rapamycin and AMPK pathways (AKT1, AKT2, FoxO1, and sirtuin 1), as well as the protein expression of cytochrome C and the apoptosis rate were upregulated. Conclusion: In conclusion, our data suggested that SCD1 was involved in enhancing the PA tolerance of goose primary hepatocytes by regulating inflammation- and apoptosis-related genes expression.

Influence of Frequency on Electromagnetic Field of Super High-Speed Permanent Magnet Generator

Qiu, Hongbo,Wei, Yanqi,Wang, Wei,Tang, Bingxia,Zhao, Xifang,Yang, Cunxiang The Korean Institute of Power Electronics 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.4

When compared with traditional power frequency generators, the frequency of a super high-speed permanent magnet generator (SHSPMG) is a lot higher. In order to study the influence of frequency on the electromagnetic field of SHSPMGs, a 60000rpm, 117kW SHSPMG was taken as a research object. The two-dimensional finite element model of the generator was established, and the two-dimensional transient field of the generator was simulated. In addition, a test platform of the generator was set up and tested. The reliability of the simulation was verified by comparing the experiment data with that of the simulation. Then the generator electromagnetic field under different frequencies was studied, and the influence mechanism of frequency on the generator electromagnetic field was revealed. The generator loss, voltage regulation rate, torque and torque ripple were analyzed under the rated active power load and different frequencies. The influences of frequency on the eddy current density, loss, voltage regulation rate and torque ripple of the generator were obtained. These conclusions can provide some reference for the design and optimization of SHSPMGs.

Tool life evaluation of CFRP drilling with three kinds of drill

Xinyi Qiu,Pengnan Li,Changping Li,Qiulin Niu,Shujian Li,Tae Jo Ko,Lingyan Tang 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.7

Serious tool wear in CFRP drilling is one of the key problems to be solved urgently. Firstly, a suitable indirect evaluation index of tool life is selected according to the literature. The critical delamination force was obtained by blind hole pushing experiment. Then, tool wear experiments were carried out with double point angle drill, stepped drill and reverse edge compound drill to analyze the variation rules of the thrust force, exit burr, exit delamination and tear with tool wear. Threshold values of exit delamination, tearing and critical thrust force were compared with each evaluation index one by one to study the causes of drills failure. The results indicated that the maximum tool wear position was the outer corner. Among the three types drills, the thrust force, delamination factor and burr angle of the reverse edge compound drill are smaller. The double point angle drill fails due to the excessive thrust force, while the stepped drill and the reverse edge compound drill fail due to the hole exit delamination exceeding the threshold. The number of drilled holes of the reverse edge compound drill is 100 % and 25 % higher than that of the double point angle drill and the stepped drill, respectively. Therefore, the reverse edge compound drill is suitable for drilling CFRP.