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Qiang He,Byung-Kook Yun,Min-Young Yoon,Xiao-Qiang Wang,Feng-Peng Li,Aye-Aye Khaing,Won-Hee Ra,Win Htet Oo,Cheol-Soon Park,Sun-Kyung Min,Wei Tong,Jie Yu,Bu-Woong Choi,Eun-Beom Heo,Khandakar Md.Rayhanul 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
As one of the most important crop, rice is not only a staple food of half world’s population but a wonderful model plant, which has been leading the evolution and functional genomics study. The next-generation sequencing technology are expediting rice genomic study, by providing a simple but powerful way. In this study, we re-sequenced a core collection of 137 rice accessions from all over the world along with 158 Korean breeding varieties. Finally, 6.3G uniquely mapped reads were obtained, and about 10 million SNPs and ~1.2 million InDels were identified with average sequencing depth of 7.5X. These will help us to maximize our germplasm utilization and assists all the deep research in population dynamics and functional studies. Here, we’d like to show the approaches applied to resequencing data mining and on-going activities.
MicroRNA-214-mediated UBC9 expression in glioma
( Zhi Qiang Zhao ),( Xiao Chao Tan ),( Ani Zhao ),( Li Yuan Zhu ),( Bin Yin ),( Jiang Ang Yuan ),( Bo Qin Qiang ),( Xiao Zhong Peng ) 생화학분자생물학회(구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.11
It has been reported that ubiquitin-conjugating enzyme 9 (Ubc9), the unique enzyme2 in the sumoylation pathway, is up-regulated in many cancers. However, the expression and regulation of UBC9 in glioma remains unknown. In this study, we found that Ubc9 was up-regulated in glioma tissues and cell lines compared to a normal control. UBC9 knockdown by small interfering RNA (siRNA) affected cell proliferation and apoptosis in T98G cells. Further experiments revealed that microRNA (miR)-214 directly targeted the 3` untranslated region (UTR) of UBC9 and that there was an inverse relationship between the expression levels of miR-214 and UBC9 protein in glioma tissues and cells. MiR-214 overexpression suppressed the endogenous UBC9 protein and affected T98G cell proliferation. These findings suggest that miR-214 reduction facilitates UBC9 expression and is involved in the regulation of glioma cell proliferation.
Liu Peng,Liu Wen,Li Qiang,Yang Li,Duan Min,Dai Yue 보안공학연구지원센터 2014 International Journal of u- and e- Service, Scienc Vol.7 No.6
For the problem that the use effect of food processing information system is poor due to absence of knowledge acquisition measures and knowledge self-updating function, a knowledge acquisition approach based on rough set is put forward. First, the approach establishes a set of predicted samples for the relationship between food processing parameters and product quality; then uses the discretization of continuous attribute, attribute reduction and rule extraction algorithm of rough set to acquire automatically predicted knowledge from a large number of predicted sample sets, and then saves the predicted knowledge in the knowledge base of expert system; finally, realizes the extraction of knowledge of food processing process based on the inference engine, which greatly enhances the effectiveness and practicality of the acquired knowledge in online aided decision system of the food processing quality and safety.
Wei Tong,Qiang He,Xiao-Qiang Wang,Min-Young Yoon,Won-Hee Ra,Feng Peng Li,Jie Yu,Win Htet Oo,Sun-Kyung Min,Buung Choi,Eun-Beom Heo,Byoung-Kook Yun,Kyu-Won Kim,Tae-Sung Kim,Chang-Yong Lee,Yong-Jin Park 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Although the overall structure of the chloroplast genome is generally conserved, a number of sequence variations have been identified, which are valuable for plant population and evolutionary studies. Here, we constructed a chloroplast variation map of 30 landrace rice strains of Korean origin, using the Oryza rufipogon chloroplast genome (Genbank: NC_017835) as a reference. Differential distribution of single nucleotide polymorphisms (SNPs) and indels across the rice chloroplast genome is suggestive of a region-specific variation. Population structure clustering revealed the existence of two clear subgroups (indica and japonica) and an admixture group (aus). Phylogenetic analysis of the 30 landrace rice strains and six rice chloroplast references suggested and supported independent evolution of O. sativa indica and japonica. Interestingly, two “aus” type accessions, which were thought to be indica type, shared a closer relationship with the japonica type. One hypothesis is that “Korean aus” was intentionally introduced and may have obtained japonica chloroplasts during cultivation. We also calculated the nucleotide diversity of 30 accessions and compared to six rice chloroplast references, which shown a higher diversity in the indica and aus groups than in the japonica group in lower level substitution diversity.
Peng Han,Jia Lin,Wen Wang,Zhihao Liu,Yating Xiang,Ting Zhang,Qiang Liu,Xiaohu Guan,Ke Qiao,Yingchun Xie,Kuaishe Wang 대한금속ᆞ재료학회 2023 METALS AND MATERIALS International Vol.29 No.2
The high-entropy alloy particles reinforced 6061Al composite was prepared by cold spray (CS) and then modified by frictionstir processing (FSP). The microstructure evolutions, corrosion, and wear behaviors of the composites were investigated. Results showed that numerous micro-pores and cracks were distributed in the Al matrix due to adiabatic shear instability andinsufficient deformation of the deposited particles for CSed samples. The average size of uniformly distributed HEA particleswas ~ 24 μm, and the geometric necessary dislocation (GND) density reached 17.1 × 1015m−2 due to large plastic deformationduring CS. Comparatively, the micro-pores and cracks were eliminated, and many fragmented HEA particles dispersed inthe Al matrix with an average size of ~ 4 μm for FSPed sample. Note that the GND density reduced to 9.8 × 1015m−2 due todynamic recrystallization during FSP. The formation rate of the oxide film was gradually greater than that of the dissolutionrate with the samples immersion in 3.5 wt% NaCl solution from 12 to 36 h, and the main corrosion mechanism was particledissolution around micro-pores and pitting for CSed and FSPed samples, respectively. In the polarization process, the FSPedsamples exhibited low corrosion tendency and high corrosion rate due to the dense and uniform microstructure, low GNDdensity, and dispersed HEA particles. The FSPed samples had better wear resistance than the CSed samples at 25 °C and200 °C. The dominant wear mechanisms of CSed and FSPed samples were abrasion at 25 °C, while the wear mechanismsof these samples were adhesion accompanied by abrasion at 200 °C.
A Study on Sybil Attacks in Internet-of-Vehicles
Qiang Zhao,Zhiming He,Haoran Mei,Limei Peng 한국통신학회 2019 한국통신학회 학술대회논문집 Vol.2019 No.1
In this paper, we conduct a study on Sybil attacks under the environment of Internet of Vehicles (IoV). We study various types of Sybil attacks and their solutions in IoV, so as to solicit a novel algorithm to address the Sybil attack.
Qiang, Deng,Peng, Wong Wai Korean Institute of Industrial Engineers 2014 Industrial Engineeering & Management Systems Vol.13 No.2
This article describes a 2-in-1 methodology utilizing simulation optimization technique and Data Envelopment Analysis in measuring an accurate efficiency score. Given the high level of stochastic data in real environment, a novel methodology known as Data Collection Budget Allocation-Data Envelopment Analysis (DCBA-DEA) is developed. An example of the method application is shown in banking institutions. In addition to the novel approach presented, this article provides a new insight to the application domain of efficiency measurement as well as the way one conducts efficiency study.
Detection of Methanol in Power Transformer Oil Using Spectroscopy
Qiang Fu,Lei Peng,Li Li,Musong Lin,Yaohong Zhao,Shengli Li,Chen Chen 대한전기학회 2019 Journal of Electrical Engineering & Technology Vol.14 No.2
Oil-paper insulation in transformers plays an important role in the operation of power grid. At present, the aging (life assessment) of transformers are mainly conducted based upon the degree of polymerization, concentration of furfural, and the gas analysis of CO and CO 2 in the transformer oil. However, these techniques lack sensitive response, especially in the early aging of oil-paper insulation. Methanol as a new indicator to evaluate the aging of insulating paper, is getting more and more attention. An evaluation method based on the measurement of methanol concentration using ultraviolet–visible spectrometer was proposed in this work. After the extraction from transformer oils, the methanol was oxidized using potassium permanganate. Then, chromotropic acid was used to react with the oxidation products (formaldehyde) to form a mauve clathrate, fi nally, the methanol content was measured using the spectroscopy. Diff erent from traditional spectroscopy which calculate only by absorbance at the characteristic wavelength, this paper use the partial least squares method, obtain the relationship between the absorbance at selected regions and the concentration of methanol. The minimum detection limit for the proposed method was 10 ppb, whereas the correlation coeffi cient for the relationship between the absorbance and the concentration of methanol was as high as 0.99.
Peng Wang,You-min Guo,Min Liu,Yong-qian Qiang,Xiao-juan Guo,Yi-li Zhang,Xiao-Yi Duan,Qiu-Juan Zhang,Weifeng Liang 대한영상의학회 2008 Korean Journal of Radiology Vol.9 No.5
Objective: We aimed to do a meta-analysis of the existing literature to assess the accuracy of prostate cancer studies which use magnetic resonance spectroscopy (MRS) as a diagnostic tool. Materials and Methods: Prospectively, independent, blind studies were selected from the Cochrane library, Pubmed, and other network databases. The criteria for inclusion and exclusion in this study referenced the criteria of diagnostic research published by the Cochrane center. The statistical analysis was adopted by using Meta-Test version 6.0. Using the homogeneity test, a statistical effect model was chosen to calculate different pooled weighted values of sensitivity, specificity, and the corresponding 95% confidence intervals (95% CI). The summary receiver operating characteristic (SROC) curves method was used to assess the results. Results: We chose two cut-off values (0.75 and 0.86) as the diagnostic criteria for discriminating between benign and malignant. In the first diagnostic criterion, the pooled weighted sensitivity, specificity, and corresponding 95% CI (expressed as area under curve [AUC]) were 0.82 (0.73, 0.89), 0.68 (0.58, 0.76), and 83.4% (74.97, 91.83). In the second criterion, the pooled weighted sensitivity, specificity, and corresponding 95% CI were 0.64 (0.55, 0.72), 0.86 (0.79, 0.91) and 82.7% (68.73, 96.68). Conclusion: As a new method in the diagnostic of prostate cancer, MRS has a better applied value compared to other common modalities. Ultimately, large scale RCT randomized controlled trial studies are necessary to assess its clinical value. Objective: We aimed to do a meta-analysis of the existing literature to assess the accuracy of prostate cancer studies which use magnetic resonance spectroscopy (MRS) as a diagnostic tool. Materials and Methods: Prospectively, independent, blind studies were selected from the Cochrane library, Pubmed, and other network databases. The criteria for inclusion and exclusion in this study referenced the criteria of diagnostic research published by the Cochrane center. The statistical analysis was adopted by using Meta-Test version 6.0. Using the homogeneity test, a statistical effect model was chosen to calculate different pooled weighted values of sensitivity, specificity, and the corresponding 95% confidence intervals (95% CI). The summary receiver operating characteristic (SROC) curves method was used to assess the results. Results: We chose two cut-off values (0.75 and 0.86) as the diagnostic criteria for discriminating between benign and malignant. In the first diagnostic criterion, the pooled weighted sensitivity, specificity, and corresponding 95% CI (expressed as area under curve [AUC]) were 0.82 (0.73, 0.89), 0.68 (0.58, 0.76), and 83.4% (74.97, 91.83). In the second criterion, the pooled weighted sensitivity, specificity, and corresponding 95% CI were 0.64 (0.55, 0.72), 0.86 (0.79, 0.91) and 82.7% (68.73, 96.68). Conclusion: As a new method in the diagnostic of prostate cancer, MRS has a better applied value compared to other common modalities. Ultimately, large scale RCT randomized controlled trial studies are necessary to assess its clinical value.