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Jia-Jia Lin,Young-Hyun Han,Jung-Woo Kwon,Yong-Nan Xu,Yi-Bo Luo,Yu-Jin Jo,Chang-Eun Park,Jung-Kyu Baang,Suk Namgoong,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2014 Reproductive & Developmental Biology(Supplement) Vol.38 No.2s
In meiosis, Emi2 plays important role as CSF (Cytostatic Factor) to make the oocyte arrested in mII stage by the inhibition of APC/C (anaphase promoting complex/cyclosome). Once the oocyte fertilized, Emi2 was destabilized and degraded. For the degradation of Emi2, calcium signaling activate calmodulin-dependent protein kinase (CaMK) and phosphorylate emi2. Phosphorylated emi2 is recognized by polo-box domain of polo-like kinase 1 (Plk1) and further degradated by ubiquitin-dependent proteolysis. But recognition of Plk1 and emi2 is unknown. In this works, we determined the high-resolution crystal structure of polo-box domain of Plk1 and phosphorylated emi2 peptide at 1.90Å. Determined structure revealed that several unique features, including binding of Phe169 in the tyrosin-rich hydrophobic pocket. This is the first report of crystallization that Plk1-emi2 complex. Based on the complex structure, we designed the peptide analogs which pontentially inhibits recognition of Emi2 by Plk1 and assessed its biological activity in oocyte maturation and pathernogenetic activation. Injection of AB103-8, the inhibitor of Plk1 Polo-box domain, in mouse oocytes, induced the maturation arrest in GV stage and the delay in mII parthenogenetic activation. Further investigations of the mechanism that Plk1 involved into the Emi2 mII arrest are underway.
An Enhanced Reversible Data Hiding Scheme based on Histogram Modification
Lin Jia(지아린),Gil-Je Lee(이길제),Kee-Young Yoo(유기영) 대한전자공학회 2010 대한전자공학회 학술대회 Vol.2010 No.6
In 2008, Lin et al. proposed the multilevel reversible data hiding scheme based on the histogram modification but many distortions are created due to the histogram shifting method which is used in the embedding phase. In this paper, the proposed scheme combines the peak point bin with the adjacent bin of the histogram to decrease the above distortions. The expermential results show that the proposed scheme achieves the better performance than Lin et al.’s scheme in both the stego image quality and the hiding capacity.
Yu-Jia Lin,Hsiao-Ting Chang,Ming-Hwai Lin,Ru-Yih Chen,Ping-Jen Chen,Wen-Yuan Lin,Jyh-Gang Hsieh,Ying-Wei Wang,Chung-Chieh Hu,Yi-Sheng Liou,Tai-Yuan Chiu,Chun-Yi Tu,Yi-Jen Wang,Bo-Ren Cheng,Tzeng-Ji Ch 한국한의학연구원 2021 Integrative Medicine Research Vol.10 No.2
Background: Medical staff may have difficulties in using conventional medicine to manage symptoms among terminally ill patients, including adverse effects of the treatment. Traditional Chinese medicine (TCM) is regarded as a complementary or alternative medicine, and has been increasingly used in the field of palliative medicine in recent years. This study aimed to investigate the experiences of and attitudes toward using TCM among palliative care professionals, and to provide preliminary information about its use in palliative care. Methods: This was a cross-sectional survey study conducted in eight inpatient hospice wards in Taiwan between December 2014 and February 2016. The questionnaire was self-administered, and was analyzed with descriptive statistics including Pearson’s Chi-square test and Fisher’s exact test. Results: A total of 251 palliative care professionals responded to the questionnaire, of whom 89.7% and 88.9% believed that the use of TCM could improve the physical symptoms and quality of life in terminally ill patients, respectively. Overall, 59.8%, of respondents suggested that TCM had rare side effects, and 58.2% were worried that TCM could affect the liver and kidney function of patients. In total, 89.7% and 88.0% of professionals agreed there were no suitable clinical practice guidelines and educational programs, respectively, for TCM use in palliative care. Conclusions: Most of the respondents agreed there was insufficient knowledge, skills-training, and continuing education on the use of TCM in terminally ill patients in Taiwan. These results show that to address patient safety considerations, guidelines about use of TCM in palliative care should be established.
Bioremediation of Coastal Saline-Alkali Marshy Soil by the Irrigation with Pulp & Paper Wastewater
LI Jia-liang,LU Zhao-hua,TIAN Jia-yi,WANG Lin,LI Peng-hui,XIAO Zhong-feng 대전대학교 환경문제연구소 2009 환경문제연구소 논문집 Vol.13 No.-
Based on the importance of coastal saline-alkali soil remediation in Yellow River Delta, China, the Cl-、Na+ transferring tendency in soil and the saline-alkali soil eco-remediation effects were researched by measuring soil Cl-、Na+ 、soluble salt、soil respire rate and dry biomass weight of reed, etc. periodically. The results shows: the concentration of irrigated soil Cl-、Na+ and soluble salt were decreased 57.7-70%、45.7-47.1% and 53.2-59.7%, respectively, and soil salt decreasing level were little influenced by the hydraulic load, inundation depth varies in some degree, but it was influenced by dry-wet alternative irrigating mode The concentration of soil total nitrogen、organic mass、microbial respire rate, and the reed biomass above ground were increased averagely to 2.17、1.20、1.46 and 1.34 multiple respectively afterirrigation with wastewater, which have complex remediation effects on the coastal saline-alkali wetland, but there are some differences among the different irrigating crafts.
Mei-Chen Lin,Ching-Wen Lou,Jan-Yi Lin,Ting An Lin,Shih-Peng Wen,Jia-Horng Lin 한국섬유공학회 2019 Fibers and polymers Vol.20 No.2
This study combines and twists 75D polyester (PET) multi-filaments and polylactic acid (PLA) multi-filaments with twist coefficients of 2, 3, 4, 5, and 6 to form 150D PET/PLA plied yarns. The 0.08-mm-diameter stainless steel (SS) fibers are made into SS braids with a 60-tooth braid gear and a take-up gear with 60, 70, 80, 90, or 100 teeth. PET/PLA plied yarn and SS braids are then combined and electrochemically treated with an electric current of 100, 200, 300, 400, or 500 mA at 60 ℃ for 24 hours, forming the PET/PLA/SS composite bone scaffolds. PET/PLA/SS composite bone scaffolds are observed by scanning electron microscope (SEM) and energy dispersive spectroscope (EDS), and tested for weight increase rate and biocompatibility. The experiment results show that the optimal twist coefficient for PET/PLA plied yarn is 4 and the optimal tooth number on the take-up gear for SS braids is 80. SEM observation result shows that hydroxyapatite (HA) deposits on the surface of PET/PLA/SS composite bone scaffolds and attaches to the PET/PLA plied yarns. Finally, regardless of electric currents, all PET/PLA/SS composite bone scaffolds possess good biocompatibility.
Fabrication of a Biodegradable Multi-layered Polyvinyl Alcohol Stent
Mei-Chen Lin,Ching-Wen Lou,Jan-Yi Lin,Ting An Lin,Yueh-Sheng Chen,Jia-Horng Lin 한국섬유공학회 2018 Fibers and polymers Vol.19 No.8
This study aims to develop biodegradable vascular stents that prevent permanent damage caused by rejection of the immune system of the human body. Polyvinyl alcohol (PVA) yarns are twisted to enhance their strength. The twisted yarns are braided and then coated with chitosan (CS). The CS-coated PVA vascular stents are chemically crosslinked with genipin (GP) to improve their flexibility and biodegradability. Their morphological characteristics are also observed using a stereoscopic microscope, and their properties are evaluated through scanning electron microscopy, Fourier transform infrared, bending test, biodegradability test, drug release measurement, and MTT assay. Results reveal that wet PVA-CS-GP vascular stents coated with multiple CS layers can maintain a tubular structure when they are bent. After crosslinking is performed, the compressive strength of the PVA-CS-GP stents is 17.04 times higher than that of pure PVA. The weight loss rate of the PVA-CS-GP vascular stents as <3 % after 30 days. The PVA-CS-GP vascular stents composed of 0.10 % heparin sodium show a good drug release effect. Biological activity test indicates that these stents exhibit good proliferation, and our structural model verifies that they are good vascular stents.
Ting Ru Lin,Yan-Yu Lin,Ting An Lin,Jia-Ci Jhang,Ching-Wen Lou,Jia-Horng Lin 한국섬유공학회 2020 Fibers and polymers Vol.21 No.9
In countries with severe gun regulations, soldiers and police officers are commonly attacked by sharp weapons. The current personal protective equipment is too heavy, and therefore, in this study, mechanically strong Kevlar® fibers,nylon fibers, and Low-melting-point polyester (LMPET) fibers are needle punched to make thin, light, and comfortablenonwoven fabrics. Hot-baking and hot-pressing heat treatments are used for thermal bonding fibers, which subsequentlygenerate a dense and rigid surface because nonwoven fabrics are subjected to damaged structure by needle punch or aslippery state due to the fibers’ properties. The results of the air permeability, tensile strength, and quasi-static stab resistancetestings verify that the dense surface is correlated with hot-pressing heat treatment.
Ting An Lin,Yu-Chun Chuang,Jan-Yi Lin,Mei-Chen Lin,Ching-Wen Lou,Keng Siang Sim,Jia-Horng Lin 한국섬유공학회 2020 Fibers and polymers Vol.21 No.10
People have increasingly rising health consciousness in recent years and researchers are thus devoted themselvesto develop multi-functional textile products. In this study, stainless steel (SS) filaments are used for electromagnetic shieldingeffectiveness (EMSE) while polypropylene (PP) filaments are used for ultraviolet resistance and good mechanical properties. Spinning and weaving continuous formation techniques are employed to produce wrapped yarns with SS and PP filaments,after which a weaving process is employed for the preparation of SS/PP woven fabrics. The woven fabrics are tested forEMSE and UV resistance, examining the effect of the lamination-layer numbers and lamination-layer angles. Test resultsshow that the optimal EMSE and UV resistance occur when SS/PP woven fabrics are laminated with two layers at 90 °. Notonly focus on the mechanical performance, the proposed woven fabrics with good EMSE, UV resistance, and a light weight,and are good candidate for a variety of application as required. The proposed UV resistance and EMSE woven fabricssignificantly increase the additional values of traditional textiles.
Jan-Yi Lin,Mei-Chen Lin,Yan-Yu Lin,Ting An Lin,Chen-Hung Huang,Ching-Wen Lou,Jia-Horng Lin 한국섬유공학회 2021 Fibers and polymers Vol.22 No.3
In this study, the coating process is employed on metal wire to help improve the friction resistant property ofcarbon fibers, thereby provides more application feasibility. The yarn coating technique for reinforcement and woven fabricprocess are used to produce carbon/stainless steel/polyester/polypropylene/acrylic (CSPPA) woven fabrics that arecharacterized with softness and a light weight. The constituent coated yarns exhibit good conductivity after being coated witha PP layer, and likewise strengthen the woven fabrics in terms of mechanical behavior of tensile strength, elongation, bendingtorsion, creep resistance, and wear-resistant properties. The test results indicate that in the woven process, samples retaingood morphology. Due to PP sheath, the tensile strength of woven fabrics increases from 23 MPa to 42 MPa. Although thelamination layer numbers does not improve the EMI SE of woven fabrics, the EMI SE still reaches over 40 dB. Themanufacturing design proposed in this study provides an innovative finishing for carbon fibers without affecting the intrinsicproperties, and provides a greater range of application for carbon fibers.