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Hee Soo Koo,Mi Ok Lee,Pyeong Tae Ku,Su Jeong Hwang,Dong Ju Park,Hyung Suk Baik 한국미생물학회 2016 The journal of microbiology Vol.54 No.10
The molecular epidemiology of norovirus infections was studied in food handlers without any symptoms from January to December 2015 in Busan city, Korea. A total of 2,174 fecal specimens from asymptomatic food handlers were analyzed, and 2.3% (49/2,174) were norovirus-positive. Fourteen of 335 samples (4.2%) were positive in January; fifteen of 299 samples (5.0%) in February, and seven of 189 samples (3.7%) in December. However, norovirus was rarely detected in other months. From sequencing analysis, 11 genotypes (five GI and six GII genotypes) were detected. Among the 42 capid gene sequences identified, 14 were from the GI genogroup, while 28 were from the GII genogroup. The most commonly detected genotype was GII.17, comprising 15 (35.7%) of positive samples. From January 2012 to December 2015, 5,138 samples were collected from gastroenteritis patients and outbreaks in Busan. The most detected genotype in 2012, 2013, and 2014 was GII.4 (121, 24, and 12 cases, respectively), but in 2015, GII.17 (25 cases) was the most common. The GII.4 genotype was the major cause of acute gastroenteritis from 2012 to 2014, but the GII.17 genotype became the most prevalent cause in 2015. Continued epidemiological surveillance of GII.17 is needed, together with assessment of the risk of norovirus infection.
부산지역 집단 식중독 발생에서 검출된 노로바이러스의 유행양상
구희수(Hee Soo Koo),구평태(Pyeong Tae Ku),이미옥(Mi Ok Lee),백형석(Hyung Suk Baik) 한국생명과학회 2016 생명과학회지 Vol.26 No.8
최근 4년 동안 부산지역에서 2인 이상 발생된 식중독 사례를 대상으로 급성 위장관염 증상을 보인 환자 472건와 조리종사자 109건을 대상으로 유전자 분석을 한 결과 총 581건 중 71건(12.2%)에서 노로바이러스를 확인하였다. 연도별 검출현황은 2012년 18.3%(30/164건), 2013년 5.6%(6/107건), 2014년 8.0%(6/75건), 2015년 12.3%(29/235)으로 2012년과 2015년이 Norovirus 식중독에 의한 유병률이 가장 높았다. 월별 발생양상은 2월에 20건(28.2%)으로 가장 많이 발생했으며, 11월 18건(25.4%), 12월 12건(16.9%) 순이었고, 5월~8월에는 노로바이러스 환자 발생이 없어, 비교적 추운 계절에 높은 검출율을 보이는 계절적인 특징을 나타내었다. 연령별 분포는 11세~20세의 검체가 269건으로 10대의 초, 중, 고등학생의 검체가 전체의 46.3%를 차지하였다. ANOVA Scheffe 검정 결과를 통해, Norovirus의 예방 및 확산 방지를 위해 10대를 위한 위생 교육 및 식중독 예방교육을 더욱 철저히 실시해야 할 시사점을 얻었다. Norovirus genotype 분석 결과, GI genogroup은 GI-1형, GI-2형, GI-3형, GI-5형, GII genogroup은 GII-1형, GII-4형, GII-5형, GII-6형, GII-17형으로 다양한 genotype이 분포되었다. 연도별 유행하는 유전형은 2012년 GII-6형(17건), 2013년 GII-6형(3건), 2014년 GII-4형(5건), 2015년 GII-6형(18건)이 가장 우세했다. 그러나 최근 국내 뿐 아니라 전 세계적으로 GII-4형이 매우 유행하고 있으나, 부산지역은 2014년을 제외하고는 GII-6형이 우세한 genotype이라는 매우 특징적 결과를 얻었다. Norovirus is the most common causative agent of acute gastroenteritis. This study was carried out to investigate molecular epidemiology of norovirus infections from outbreaks in Busan from 2012 to 2015. Total of 581 stool specimens were collected from diarrhea patients in outbreaks in Busan, 71 samples were resulted in positive to norovirus. The data were analyzed according to seasonality, patient, age and gender. Noroviruses were detected most frequently during the winter season from November (25.4%) to February (28.2%). The age group from teens was the most susceptible to norovirus infections. To obtain the molecular genetic information of norovirus, we performed sequencing analyses of the strains detected. Norovirus genotypes have been reported to show high genetic diversity. Four kinds of GI genotypes (GI-1, GI-2, GI-3, GI-5) and five kinds of GII genotypes (GII-1, GII-4, GII-5, GII-6, GII-17) were indentified in outbreaks in Busan. Other previous studies have shown that GII-4 is the most predominant circulating in Korea and worldwide. The most prevalent norovirus genotypes of each year were GII-6 in 2012, GII-6 in 2013, GII-4 in 2014 and GII-6 in 2015. Except for 2014, GII-6 genotype was the most prevalent and predominant in Busan. We described the epidemiological analysis of the noroviruses in outbreaks in Busan. The result of this study will contribute to update the epidemiological data and improve hygiene and public health via sustainable surveillance.
구희수,Ji-young Park,Gyung-hye Sung,Eun-hee Park,Pyeong-tae Ku,Mi-ok Lee 한국수산과학회 2021 Fisheries and Aquatic Sciences Vol.24 No.8
Kudoa septempunctata has been reported as a new parasite in aquacultured olive flounder Paralichthys olivaceus, and also as a causative agent of food poisoning in humans. This paper investigated the infection of K. septempunctata in 216 sashimi and 20 sushi made of olive flounders in Busan, Korea. Among 236 samples, K. septempunctata was detected in eleven sashimi with 6–7 polar capsules by the microscopy. Among eleven sashimi, five sashimi were positive in Polymerase Chain Reaction (PCR) assay with the targets of 18S rDNA and 28S rDNA. The genotype of all the five PCR results is identified as the genotype ST3 which is common in Korea. K. septempunctata was found in olive flounders sashimi from Samcheonpo and Wando outside of Jeju Island. These findings would contribute to establish the standard of K. septempunctata for preventing food-borne outbreaks in advance and providing further preventive management for the seafood safety.