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( Ze Ping Luo ),( Hai Yan Lin ),( Wen Bing Ding ),( Hua Liang He ),( You Zhi Li ) 한국균학회 2015 Mycobiology Vol.43 No.4
Sixty-one endophytic fungus strains with different colony morphologies were isolated from the leaves, stems and roots of Tephrosia purpurea with colonization rates of 66.95%, 37.50%, and 26.92%, respectively. Based on internal transcribed spacer sequence analysis, 61 isolates were classified into 16 genera belonging to 3 classes under the phylum Ascomycota. Of the 61 isolates, 6 (9.84%) exhibited antifungal activity against one or more indicator plant pathogenic fungi according to the dual culture test. Isolate TPL25 had the broadest antifungal spectrum of activity, and isolate TPL35 was active against 5 plant pathogenic fungi. Furthermore, culture filtrates of TPL25 and TPL35 exhibited greater than 80% growth inhibition against Sclerotinia sclerotiorum. We conclude that the endophytic fungal strains TPL25 and TPL35 are promising sources of bioactive compounds.
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Ping Wang,Xiangyuan Peng,Cheng Ze 전력전자학회 2022 JOURNAL OF POWER ELECTRONICS Vol.22 No.7
The state of health (SOH) of lithium-ion batteries is a key factor to ensure the safe and reliable operation of electric vehicles (EVs). Differential thermal voltammetry (DTV) has been shown to be an effective in situ diagnosis method. In this paper, a SOH estimation method for lithium-ion batteries by a fusion of DTV and Gaussian process regression (GPR) is proposed. First, a wavelet transform (WT) is used to smooth DTV curves. Then the health features (HFs) are extracted, and their correlation with capacity loss is quantitatively evaluated through a Pearson correlation coefficient. To compress the data, canonical correlation analysis (CCA) is applied to obtain the canonical health feature (CHF). Subsequently, the GPR algorithm is used to establish the nonlinear mapping relationship between CHF and SOH. Experiments are performed on two datasets at different temperatures. Results show that the proposed method has high estimation accuracy and robustness.
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Diversity, distribution, and antagonistic activities of rhizobacteria of Panax notoginseng
Ze-Yan Fan,Cui-Ping Miao,Xin-Guo Qiao,You-Kun Zheng,Hua-Hong Chen,You-Wei Chen,Li-Hua Xu,Li-Xing Zhao,Hui-Lin Guan 고려인삼학회 2016 Journal of Ginseng Research Vol.40 No.2
Background: Rhizobacteria play an important role in plant defense and could be promising sources of biocontrol agents. This study aimed to screen antagonistic bacteria and develop a biocontrol system for root rot complex of Panax notoginseng. Methods: Pure-culture methods were used to isolate bacteria from the rhizosphere soil of notoginseng plants. The identification of isolates was based on the analysis of 16S ribosomal RNA (rRNA) sequences. Results: A total of 279 bacteria were obtained from rhizosphere soils of healthy and root-rot notoginseng plants, and uncultivated soil. Among all the isolates, 88 showed antagonistic activity to at least one of three phytopathogenic fungi, Fusarium oxysporum, Fusarium solani, and Phoma herbarum mainly causing root rot disease of P. notoginseng. Based on the 16S rRNA sequencing, the antagonistic bacteria were characterized into four clusters, Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetesi. The genus Bacillus was the most frequently isolated, and Bacillus siamensis (Hs02), Bacillus atrophaeus (Hs09) showed strong antagonistic activity to the three pathogens. The distribution pattern differed in soil types, genera Achromobacter, Acidovorax, Brevibacterium, Brevundimonas, Flavimonas, and Streptomyces were only found in rhizosphere of healthy plants, while Delftia, Leclercia, Brevibacillus, Microbacterium, Pantoea, Rhizobium, and Stenotrophomonas only exist in soil of diseased plant, and Acinetobacter only exist in uncultivated soil. Conclusion: The results suggest that diverse bacteria exist in the P. notoginseng rhizosphere soil, with differences in community in the same field, and antagonistic isolates may be good potential biological control agent for the notoginseng root-rot diseases caused by F. oxysporum, Fusarium solani, and Panax herbarum.
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Ze-ying Liu,Xu-ping Gao,Sui Zhu,Yan-hua Liu,Li-jun Wang,Chun-xia Jing,Fang-fang Zeng 대한부인종양학회 2019 Journal of Gynecologic Oncology Vol.30 No.3
Objective: There has been growing body of literatures showing that chronic inflammation might play an important role in cancer development. This meta-analysis aimed to assess the association between the dietary inflammation index (DII) score and gynecological cancers. Methods: A systematic search of PubMed, EMBASE and Web of Science up until October 20, 2018 was carried out to retrieve all related cohort and case-control studies. The summary risk assessments were pooled using random-effects models. The dose-response relationship was estimated by linear relationship model. Results: Twelve case-control studies (10,774 cases/15,958 controls) and six prospective cohort studies (330,363 participants/23,133 incident cases) were included in this meta-analysis. The pooled adjusted relative risk (RR) of gynecological cancers for the highest DII category compared to the lowest category was 1.38, (95% confidence intervals [CIs], 1.21–1.56, p<0.001]. A positive dose-response relationship was also noticed. Stratified by study design indicated that, the pooled RRs was significantly higher for case-control studies than cohort studies (p for interaction<0.001), for studies conducted among participants with body mass index (BMI) ≥25 kg/m2 than participants with BMI <25 kg/m2 (p for interaction=0.026), among participants with ovarian cancer and endometrial cancer than participants with breast cancer (p for interaction = 0.038). Meta-regression analysis further confirmed that study design significantly contributed to inter-study heterogeneity (p<0.001). Conclusion: This meta-analysis suggests that elevated DII is independently associated with a higher risk of gynecological cancers, especially patients with ovarian cancer and endometrial cancer and among obese participants.
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Ze-Jian Wang,Xingzi Zhang,Ping Wang,Zhiwei Sui,Meijin Guo,Siliang Zhang,Yingping Zhuang 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.3
The effects of different oxygen uptake rates (OUR) on the physiological metabolism of Rhodobacter sphaeroides were investigated systematically in 50 L fermenters due to the significant influence on industrial coenzyme Q10 production under oxygen supply limitation. Meanwhile, the seriously decreased oxygen transfer rate caused by the increased broth viscosity was successfully prevented with OUR-directed continuous ammonium sulfate feeding in the late fermentation phase. The statistical analysis results showed that controlling OUR constantly at 45 ± 2.2 mmol/L/h by the oxygen supply level adjustment and the continuous ammonium sulfate feeding could greatly enhance Q10 production. This OUR-Stat controlling strategy successfully achieved the maximal coenzyme Q10 production (2584 ± 82 mg/L), which was 15.4% higher than that of the control. The highest specific CoQ10 content was 25.9 mg/(g DCW)), and the yield of CoQ10 to glucose consumption was up to 19.37 mg/g. These results demonstrated that the optimal OUR-Stat controlling strategy would be effective and economical for improving the industrial CoQ10 production.
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Qin Ping Yu,Ding Yuan Feng,Juan Xiao,Fan Wu,Xiao Jun He,Min Hao Xia,Tao Dong,Yi Hua Liu,Hui Ze Tan,Shi Geng Zou,Tao Zheng,Xian Hua Ou,Jian Jun Zuo 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.12
Objective: This experiment investigated meat color, myoglobin content, enzyme activities, and expression of genes associated with oxidative potential of pigs slaughtered at different growth stages. Methods: Sixty 4-week-old Duroc×Landrace×Yorkshire pigs were assigned to 6 replicate groups, each containing 10 pigs. One pig from each group was sacrificed at day 35, 63, 98, and 161 to isolate longissimus dorsi and triceps muscles. Results: Meat color scores were higher in pigs at 35 d than those at 63 d and 98 d (p<0.05), and those at 98 d were lower than those at 161 d (p<0.05). The total myoglobin was higher on 161 d compared with those at 63 d and 98 d (p<0.05). Increase in the proportions of metmyoglobin and deoxymyoglobin and a decrease in oxymyoglobin were observed between days 35 and 161 (p<0.05). Meat color scores were correlated to the proportion of oxymyoglobin (r = 0.59, p<0.01), and negatively correlated with deoxymyoglobin and metmyoglobin content (r = –0.48 and –0.62, p<0.05). Malate dehydrogenase (MDH) activity at 35 d and 98 d was higher than that at 161 d (p<0.05). The highest lactate dehydrogenase/MDH ratio was achieved at 161 d (p<0.05). Calcineurin mRNA expression decreased at 35 d compared to that at 63 d and 98 d (p<0.05). Myocyte enhancer factor 2 mRNA results indicated a higher expression at 161 d than that at 63 d and 98 d (p<0.05). Conclusion: Porcine meat color, myoglobin content, enzyme activities, and genes associated with oxidative potential varied at different stages.
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Diversity, distribution, and antagonistic activities of rhizobacteria of Panax notoginseng
Fan, Ze-Yan,Miao, Cui-Ping,Qiao, Xin-Guo,Zheng, You-Kun,Chen, Hua-Hong,Chen, You-Wei,Xu, Li-Hua,Zhao, Li-Xing,Guan, Hui-Lin The Korean Society of Ginseng 2016 Journal of Ginseng Research Vol.40 No.2
Background: Rhizobacteria play an important role in plant defense and could be promising sources of biocontrol agents. This study aimed to screen antagonistic bacteria and develop a biocontrol system for root rot complex of Panax notoginseng. Methods: Pure-culture methods were used to isolate bacteria from the rhizosphere soil of notoginseng plants. The identification of isolates was based on the analysis of 16S ribosomal RNA (rRNA) sequences. Results: A total of 279 bacteria were obtained from rhizosphere soils of healthy and root-rot notoginseng plants, and uncultivated soil. Among all the isolates, 88 showed antagonistic activity to at least one of three phytopathogenic fungi, Fusarium oxysporum, Fusarium solani, and Phoma herbarum mainly causing root rot disease of P. notoginseng. Based on the 16S rRNA sequencing, the antagonistic bacteria were characterized into four clusters, Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetesi. The genus Bacillus was the most frequently isolated, and Bacillus siamensis (Hs02), Bacillus atrophaeus (Hs09) showed strong antagonistic activity to the three pathogens. The distribution pattern differed in soil types, genera Achromobacter, Acidovorax, Brevibacterium, Brevundimonas, Flavimonas, and Streptomyces were only found in rhizosphere of healthy plants, while Delftia, Leclercia, Brevibacillus, Microbacterium, Pantoea, Rhizobium, and Stenotrophomonas only exist in soil of diseased plant, and Acinetobacter only exist in uncultivated soil. Conclusion: The results suggest that diverse bacteria exist in the P. notoginseng rhizosphere soil, with differences in community in the same field, and antagonistic isolates may be good potential biological control agent for the notoginseng root-rot diseases caused by F. oxysporum, Fusarium solani, and Panax herbarum.
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Dynamics of excitons and carriers at the NPB/C60 interface by transient photocurrents
Li Ping,De Yang Xiu,Wu Bo,Zhang Yu,Jiang Ze Zhuan,Bao Xi,Huang Hai Shen 한국물리학회 2020 Current Applied Physics Vol.20 No.5
The transient photocurrent (TPC) technique was performed to explore the dynamics of excitons and carriers at organic active layer/buffer layer interfaces. A special device with ITO/PEIE/NPB/C60/Al structure was designed to study the interfacial processes at the NPB/C60 interface. An external electrical field was provided to neutralize the built-in electrical field of the device. Interestingly, a new phenomenon was observed, wherein the polarity of the TPC changed from negative to positive under an external electrical field. The initial negative signal was ascribed to exciton separation by the built-in field in C60, and the subsequent positive signal can be attributed to the diffusion of electrons that accumulate at the NPB/C60 interface. TPC measurements shown that further increasing the external electrical field causes polarity to change twice. Analyzing the two changes in polarity revealed that the NPB did not only extract holes from C60 but also provided an effective interface for exciton dissociation.
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